{"id":44437,"date":"2023-07-27T11:24:54","date_gmt":"2023-07-27T03:24:54","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=44437"},"modified":"2025-08-13T14:22:56","modified_gmt":"2025-08-13T06:22:56","slug":"%e9%81%a9%e7%94%a8%e6%96%bc%e5%93%ba%e4%b9%b3%e5%8b%95%e7%89%a9%e7%b4%b0%e8%83%9e-%e7%b5%84%e7%b9%94%e7%9a%84-minute-%e7%b8%bd%e8%84%82%e7%ad%8f%e5%88%86%e9%9b%a2%e8%a9%a6%e5%8a%91%e7%9b%92","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=44437","title":{"rendered":"\u9069\u7528\u65bc\u54fa\u4e73\u52d5\u7269\u7d30\u80de\/\u7d44\u7e54\u7684 Minute\u2122 \u7e3d\u8102\u7b4f\u5206\u96e2\u8a66\u5291\u76d2 |Total Lipid Raft Isolation Kit for Mammalian Cells\/Tissues Invent\u8ca8\u865fLR-039"},"content":{"rendered":"<p><strong><span style=\"font-size: 14pt; color: #800000;\">\u5feb\u901f\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u7cfb\u5217-<span style=\"color: #ff9900;\">Rapid Protein Extraction Kits<\/span><\/span><\/strong><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/Invent-minute_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1.jpg\" \/><\/p>\n<p><strong>Minute\u2122<\/strong><strong>\u86cb\u767d\u8403\u53d6\u5957\u7d44\uff0c\u4f7f\u7528\u5275\u65b0\u300eColumn Filter\u300f\u6280\u8853\uff0c\u6548\u679c\u66f4\u4f73\uff0c\u5b8c\u6210\u53ea\u9700<\/strong><strong>\u00a0<\/strong><strong>\u4e00 \u5206\u9418<\/strong><strong>\u86cb\u767d\u8403\u53d6\u53ea\u9700\u8981<em>vortex\u3001transfer\u3001spin \u3002<\/em><\/strong>\u907f\u514d RIPA \u4e0d\u6eb6\u6027\u86cb\u767d\u8cea\u7684\u554f\u984c\u57fa\u65bc\u65cb\u8f49\u67f1\u7684\u65b9\u6cd5\u7372\u5f97\u66f4\u5b8c\u6574\u7684\u86cb\u767d\u8cea\u8b5c\u3002Minute \u2122\u4e00\u7cfb\u5217\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\uff1b\u9069\u7528\u65bc\u591a\u7a2e\u7269\u7a2e\uff08\u5305\u62ec\u54fa\u4e73\u52d5\u7269\u3001\u6d77\u6d0b\u52d5\u7269\u3001\u6606\u87f2\u3001\u690d\u7269\u548c\u7d30\u83cc\uff09\u5feb\u901f\u548c\u5bb9\u6613\u5730\u63d0\u53d6\/\u5206\u96e2\u86cb\u767d\u8cea\u7684\u512a\u7570\u5de5\u5177 \u3002\u8207\u5e02\u5834\u4e0a\u7684\u4efb\u4f55\u5176\u4ed6\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u4e0d\u540c\uff0c\u512a\u5316\u7684\u7de9\u885d\u6db2\u7cfb\u7d71\uff0c\u7d50\u5408\u6700\u65b0\u7684\u5c08\u6709\u6ffe\u7b52\u6280\u8853\uff08spin column\uff09\uff1b\u5feb\u901f\u63d0\u9ad8\u86cb\u767d\u8403\u53d6\u7684\u6548\u80fd\u53ca\u65b9\u4fbf\u6027\uff1b\u7528\u65bc\u7e3d\u86cb\u767d\u63d0\u53d6\uff0c\u7d30\u80de\u5206\u96e2\u548c\u819c\u86cb\u767d\u5206\u96e2\uff0c\u53ea\u9700\u7c21\u55ae\u56db\u6b65\u9a5f\u5373\u53ef\u5b8c\u6210\u86cb\u767d\u8cea\u8403\u53d6\u3002\u7121\u9808\u9577\u6642\u9593\u7b49\u5f85\u3001\u514d\u9664\u91cd\u8907\u6027\u7684wash\u6b65\u9a5f\u3002<\/p>\n<p><iframe loading=\"lazy\" title=\"Minute Total Protein Extraction Kit - Invent Biotechnologies\" src=\"\/\/www.youtube.com\/embed\/n0RpoqGC2IY\" width=\"562\" height=\"316\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\" data-mce-fragment=\"1\"><\/iframe><\/p>\n<p><a href=\"https:\/\/biopioneer.com.tw\/?p=44437&amp;preview=true\"><strong><span style=\"color: #33cccc; font-size: 12pt;\">\u9069\u7528\u65bc\u54fa\u4e73\u52d5\u7269\u7d30\u80de\/\u7d44\u7e54\u7684 Minute\u2122 \u7e3d\u8102\u7b4f\u5206\u96e2\u8a66\u5291\u76d2 | Invent\u8ca8\u865fLR-039<\/span><\/strong><\/a><\/p>\n<p><span style=\"color: #999999; font-size: 12pt;\">Minute\u2122 Total Lipid Raft Isolation Kit for Mammalian Cells\/Tissues<\/span><\/p>\n<p><strong><a href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/39-LR-039_v8.pdf?v=1645819787\" target=\"_blank\" rel=\"noopener\">\u624b\u518a\u548c\u5354\u8b70<\/a>\u00a0|\u00a0<a href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-6_3de71b67-275f-4902-beb7-9b90b40902af.pdf?7628830165208314739\" target=\"_blank\" rel=\"noopener\">\u6750\u6599\u5b89\u5168\u6578\u64da\u8868 (MSDS)<\/a><\/strong><\/p>\n<p>\u8102\u7b4f\u662f\u542b\u6709\u9ad8\u6c34\u5e73\u81bd\u56fa\u9187\u548c\u9798\u8102\u7684\u5c0f\u819c\u57df\u3002\u8102\u7b4f\u5b58\u5728\u65bc\u8cea\u819c\uff08PM\uff09\u548c\u5167\u90e8\u7d30\u80de\u5668\u819c\u4e2d\uff0c\u4f8b\u5982\u7dda\u7c92\u9ad4\u76f8\u95dc\u819c\uff08MAM\uff09\u548c\u5167\u8cea\u7db2\u3002\u5b83\u5011\u6d89\u53ca\u8a31\u591a\u7d30\u80de\u904e\u7a0b\uff0c\u5305\u62ec\u4f46\u4e0d\u9650\u65bc\u4fe1\u865f\u8f49\u5c0e\u3001\u819c\u904b\u8f38\u548c\u86cb\u767d\u8cea\u5206\u9078\u3002\u8102\u8cea\u4fee\u98fe\u86cb\u767d\u548c\u4e00\u4e9b\u8de8\u819c\u86cb\u767d\u96c6\u4e2d\u5728\u7b4f\u4e2d\uff0c\u800c\u5176\u4ed6\u86cb\u767d\u5247\u88ab\u6392\u9664\u5728\u5916\u3002<sup>\u8102\u7b4f\u4e5f\u88ab\u767c\u73fe\u8207 Na +<\/sup>\u00a0\/K\u00a0<sup>+<\/sup>\u6709\u95dc\u8cea\u819c\u4e0a\u7684 ATP \u9176\u3002\u50b3\u7d71\u4e0a\u4f7f\u7528\u9700\u8981\u5927\u91cf\u8d77\u59cb\u6750\u6599\u7684\u8d85\u901f\u96e2\u5fc3\u901a\u904e\u8517\u7cd6\u68af\u5ea6\u6216 OptiPrep \u68af\u5ea6\u5206\u96e2\u8102\u7b4f\u3002\u8a72\u5354\u8b70\u7e41\u7463\u4e14\u8017\u6642\u3002\u8a72\u8a66\u5291\u76d2\u662f\u4f7f\u7528\u6211\u5011\u5c08\u6709\u7684\u57fa\u65bc\u65cb\u8f49\u67f1\u7684\u6280\u8853\u958b\u767c\u7684\uff0c\u63d0\u4f9b\u4e86\u4e00\u7a2e\u5feb\u901f\u3001\u7c21\u55ae\u7684\u65b9\u6cd5\u4f86\u5206\u96e2\u8102\u7b4f\u3002\u9996\u5148\u5206\u96e2\u7e3d\u819c\u90e8\u5206\uff08\u5305\u62ec PM \u548c\u7d30\u80de\u5668\u819c\uff09\uff0c\u4e26\u7528\u542b\u6709\u975e\u96e2\u5b50\u53bb\u6c61\u5291\u7684\u7de9\u885d\u6db2\u8655\u7406\uff0c\u7136\u5f8c\u50c5\u4f7f\u7528\u53f0\u5f0f\u5fae\u91cf\u96e2\u5fc3\u6a5f\u901a\u904e\u6d6e\u9078\u96e2\u5fc3\u5206\u96e2\u8010\u53bb\u6c61\u5291\u7684\u90e8\u5206\u3002\u7121\u9700\u4f7f\u7528\u5bc6\u5ea6\u68af\u5ea6\u548c\u8d85\u901f\u96e2\u5fc3\uff0c\u5373\u53ef\u5728 90 \u5206\u9418\u5167\u5f9e\u57f9\u990a\u7684\u7d30\u80de\/\u7d44\u7e54\u4e2d\u7372\u5f97\u9ad8\u5ea6\u5bcc\u96c6\u7684\u7e3d\u8102\u7b4f\u3002<\/p>\n<p><em><strong>*\u6709\u95dc\u8cea\u819c\u884d\u751f\u8102\u7b4f\u7684\u5206\u96e2\uff0c\u8acb\u53c3\u95b1 Minute\u00a0<sup>TM<\/sup>\u00a0\u00a0Plasma Membrane-Derived Lipid Raft Isolation Kit\uff0c\u76ee\u9304<\/strong><\/em><strong><em><a href=\"https:\/\/inventbiotech.com\/products\/minute%E2%84%A2-plasma-membrane-derived-lipid-raft-isolation-kit-20-preps?_pos=1&amp;_sid=9cbfd6e42&amp;_ss=r\" target=\"_blank\" rel=\"noopener\">\u865f\uff1aLR-042<\/a><\/em><\/strong><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><a href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Comparison_of_Major_Lipid_Raft_Isolation_Kits.pdf?824\" target=\"_blank\" rel=\"noopener\"><strong>\u8207\u5176\u4ed6\u8102\u7b4f\u5206\u96e2\u8a66\u5291\u76d2\u6bd4\u8f03<\/strong><\/a><\/p>\n<p>Lipid rafts are small membrane domains containing a high level of cholesterol and sphingolipids.\u00a0 Lipid rafts are found in the plasma membrane (PM) and internal organellar membranes such as mitochondria-associated membrane (MAMs) and endoplasmic reticulum. They have been implicated in numerous cellular processes including but not limited to signal transduction, membrane trafficking, and protein sorting. Lipid-modified proteins and some transmembrane proteins are concentrated in the rafts while other proteins are excluded. Lipid rafts are also found to be associated with Na<sup>+<\/sup>\/K<sup>+<\/sup>\u00a0ATPase on the plasma membrane. Lipid rafts are isolated by sucrose-gradient or OptiPrep gradient traditionally using ultracentrifugation that requires a large amount of starting material. The protocol is tedious and time-consuming. This kit was developed using our proprietary spin-column-based technologies, offering a rapid and easy way to isolate lipid rafts. Total membrane fraction (include PM and organelle membranes) is first isolated and treated with a non-ionic detergent containing buffer, followed by isolation of detergent-resistant fraction by flotation centrifugation using just a tabletop microcentrifuge. Highly enriched total lipid rafts can be obtained from cultured cells\/tissues in less than 90 min without using density gradient and ultracentrifugation.<\/p>\n<p><em><strong>*For isolation of plasma membrane-derived lipid rafts, please refer to Minute<sup>TM<\/sup>\u00a0Plasma Membrane-Derived Lipid Raft Isolation Kit under\u00a0<\/strong><\/em><strong><em>\u00a0<a href=\"https:\/\/inventbiotech.com\/products\/minute%E2%84%A2-plasma-membrane-derived-lipid-raft-isolation-kit-20-preps?_pos=1&amp;_sid=9cbfd6e42&amp;_ss=r\" target=\"_blank\" rel=\"noopener\">Cat #: LR-042<\/a><\/em><\/strong><\/p>\n<p><a href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Comparison_of_Major_Lipid_Raft_Isolation_Kits.pdf?824\" target=\"_blank\" rel=\"noopener\"><strong>Compare with Other Lipid Raft Isolation kits<\/strong><\/a><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/%E9%81%A9%E7%94%A8%E6%96%BC%E5%93%BA%E4%B9%B3%E5%8B%95%E7%89%A9%E7%B4%B0%E8%83%9E%E7%B5%84%E7%B9%94%E7%9A%84-Minute%E2%84%A2-%E7%B8%BD%E8%84%82%E7%AD%8F%E5%88%86%E9%9B%A2%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FLR-039-5.webp\" \/><\/p>\n<p><strong>Evaluation of Cross-Contamination of Isolated Lipid Rafts<\/strong><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/%E9%81%A9%E7%94%A8%E6%96%BC%E5%93%BA%E4%B9%B3%E5%8B%95%E7%89%A9%E7%B4%B0%E8%83%9E%E7%B5%84%E7%B9%94%E7%9A%84-Minute%E2%84%A2-%E7%B8%BD%E8%84%82%E7%AD%8F%E5%88%86%E9%9B%A2%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FLR-039-4.webp\" \/><\/p>\n<p>\u5f9e\u5c0f\u9f20\uff08TR\uff0c\u5de6\uff09\u548c\u4eba\uff08\u53f3\uff09\u76ae\u8cea\u4e2d\u5206\u96e2\u51fa\u8102\u7b4f\uff0c\u4e26\u7528 H3 \u548c GAPDH \u9032\u884c\u63a2\u6e2c\u3002\u8102\u7b4f\u90e8\u5206\u4e2d\u4e0d\u5b58\u5728\u4efb\u4f55\u4e00\u7a2e\u3002\u4eba\u7c97\u6838\u63d0\u53d6\u7269\u542b\u6709\u4e00\u4e9b GAPDH\uff0c\u53ef\u80fd\u4f86\u81ea\u7d05\u7d30\u80de\u3002\u6578\u64da\uff1a\u7531\u5357\u52a0\u5dde\u5927\u5b78 Max Thorwald \u535a\u58eb\u63d0\u4f9b\u3002<\/p>\n<p><strong>\u5206\u96e2\u8102\u7b4f\u4e2d\u8102\u7b4f\u6a19\u8a18\u7269\u7684\u6aa2\u6e2c<\/strong><\/p>\n<p>Lipid rafts were isolated from mouse (TR, left) and human (right) cortex that were probed with H3 and GAPDH. No presence of either in the lipid raft fraction. Human crude nuclear extract has some GAPDH, likely from RBCs. Data: Courtesy of Dr. Max Thorwald, University of Southern California.<\/p>\n<p><strong>Detection of Lipid Raft Marker in Isolated Lipid Rafts<\/strong><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/%E9%81%A9%E7%94%A8%E6%96%BC%E5%93%BA%E4%B9%B3%E5%8B%95%E7%89%A9%E7%B4%B0%E8%83%9E%E7%B5%84%E7%B9%94%E7%9A%84-Minute%E2%84%A2-%E7%B8%BD%E8%84%82%E7%AD%8F%E5%88%86%E9%9B%A2%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FLR-039-3.webp\" \/><\/p>\n<p>\u4f7f\u7528 Flotillin-1 \u63a2\u6e2c\u5f9e\u5c0f\u9f20\uff08TR\uff0c\u5de6\uff09\u548c\u4eba\u76ae\u5c64\uff08\u53f3\uff09\u5206\u96e2\u8a66\u5291\u76d2\u4e2d\u7372\u5f97\u7684\u8102\u7b4f\u7d44\u5206\u3002\u8102\u7b4f\u7d44\u5206\u4e2d Flotillin-1 \u5448\u967d\u6027\u3002\u6578\u64da\uff1a\u7531\u5357\u52a0\u5dde\u5927\u5b78 Max Thorwald \u535a\u58eb\u63d0\u4f9b\u3002<\/p>\n<p><strong>\u8102\u7b4f\u63d0\u53d6\u8a66\u5291\u76d2\u8207\u8d85\u901f\u96e2\u5fc3\u7684\u6bd4\u8f03<\/strong><\/p>\n<p>Lipid raft fractions obtained from isolation kit for mouse (TR, left) and human cortex (right) were probed with Flotillin-1. Lipid raft fractions are positive for Flotillin-1.\u00a0 Data: Courtesy of Dr. Max Thorwald, University of Southern California.<\/p>\n<p><strong>Comparison of Lipid Raft Extractions kit with Ultracentrifugation<\/strong><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/%E9%81%A9%E7%94%A8%E6%96%BC%E5%93%BA%E4%B9%B3%E5%8B%95%E7%89%A9%E7%B4%B0%E8%83%9E%E7%B5%84%E7%B9%94%E7%9A%84-Minute%E2%84%A2-%E7%B8%BD%E8%84%82%E7%AD%8F%E5%88%86%E9%9B%A2%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FLR-039-2.webp\" \/><\/p>\n<p>\u4f7f\u7528\u5de6\u5074\u7684 Flotillin-1 \u63a2\u6e2c\u6a23\u54c1\u3002\u6240\u6709\u8102\u7b4f\u6a23\u54c1\u5747\u5728\u53f3\u5074\u63a2\u6e2c H3 \u548c GAPDH \u6a23\u54c1\u3002\u8a72\u5206\u96e2\u8a66\u5291\u76d2\u7522\u751f\u5bcc\u96c6\u7684\u8102\u7b4f\u7d44\u5206\uff0c\u4e26\u4e14\u6c92\u6709\u53ef\u898b\u7684 H3 \u6216 GAPDH \u5b58\u5728\u3002\u6578\u64da\uff1a\u7531\u5357\u52a0\u5dde\u5927\u5b78 Max Thorwald \u535a\u58eb\u63d0\u4f9b\u3002<\/p>\n<p>*TR=\u8f49\u57fa\u56e0\u5c0f\u9f20\u76ae\u8cea\uff1b\u4eba\u985e=\u4eba\u985e\u76ae\u8cea<\/p>\n<p>Samples were probed with Flotillin-1 on the left. All lipid raft samples probed for H3 and GAPDH samples on the right. The isolation kit yields enriched lipid raft fractions and no visible presence of H3 or GAPDH. Data: Courtesy of Dr. Max Thorwald, University of Southern California.<\/p>\n<p>*TR=transgenic mouse cortex; Human=Human Cortex<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/%E9%81%A9%E7%94%A8%E6%96%BC%E5%93%BA%E4%B9%B3%E5%8B%95%E7%89%A9%E7%B4%B0%E8%83%9E%E7%B5%84%E7%B9%94%E7%9A%84-Minute%E2%84%A2-%E7%B8%BD%E8%84%82%E7%AD%8F%E5%88%86%E9%9B%A2%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FLR-039.webp\" \/><\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>\u9069\u7528\u65bc\u54fa\u4e73\u52d5\u7269\u7d30\u80de\/\u7d44\u7e54\u7684 Minute\u2122 \u7e3d\u8102\u7b4f\u5206\u96e2\u8a66\u5291 Kit includes:<\/strong><\/span><\/p>\n<table>\n<tbody>\n<tr>\n<td><strong>Items<\/strong><\/td>\n<td><strong>Quantity<\/strong><\/td>\n<\/tr>\n<tr>\n<td>Buffer A<\/td>\n<td>15 ml<\/td>\n<\/tr>\n<tr>\n<td>Buffer B<\/td>\n<td>10 ml<\/td>\n<\/tr>\n<tr>\n<td>Buffer C<\/td>\n<td>10 ml<\/td>\n<\/tr>\n<tr>\n<td>Filter Cartridges<\/td>\n<td>20 units<\/td>\n<\/tr>\n<tr>\n<td>Collection Tubes (2 ml)<\/td>\n<td>20 units<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/%E9%81%A9%E7%94%A8%E6%96%BC%E5%93%BA%E4%B9%B3%E5%8B%95%E7%89%A9%E7%B4%B0%E8%83%9E%E7%B5%84%E7%B9%94%E7%9A%84-Minute%E2%84%A2-%E7%B8%BD%E8%84%82%E7%AD%8F%E5%88%86%E9%9B%A2%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FLR-039-6.webp\" width=\"408\" height=\"407\" \/><\/p>\n<p><strong><a href=\"https:\/\/inventbiotech.com\/\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/03\/%E5%8E%9F%E5%BB%A0%E9%80%A3%E7%B5%9001-300x79.png\" alt=\"\u539f\u5ee0\u9023\u7d5001\" width=\"138\" height=\"36\" \/><\/a><\/strong><strong><a href=\"https:\/\/www.abmgood.com\/\"><img decoding=\"async\" src=\"https:\/\/www.abmgood.com\/pub\/media\/logo\/websites\/1\/abm-logo-high-res.png\" alt=\"\" \/><\/a><img decoding=\"async\" src=\"https:\/\/www.abmgood.com\/pub\/media\/logo\/websites\/1\/abm-logo-high-res.png\" alt=\"\" \/><img decoding=\"async\" src=\"https:\/\/www.abmgood.com\/pub\/media\/logo\/websites\/1\/abm-logo-high-res.png\" alt=\"\" \/><\/strong><\/p>\n<p><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\">\u57fa\u65bc\u65cb\u8f49\u67f1(SPIN-COLUMN-BASED )\u7684\u86cb\u767d\u8cea\u63d0\u53d6\/\u7d30\u80de\u5206\u7d1a(CELL FRACTIONATION)<\/span><\/strong><\/span><\/p>\n<p><strong><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-4356\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2015\/02\/Compined-F.gif\" alt=\"Compined-F\" width=\"446\" height=\"167\" \/><\/strong><\/p>\n<p>Invent Biotechnologies \u6210\u7acb\u65bc 2010 \u5e74\uff0c\u56e0\u9769\u65b0\u86cb\u767d\u8cea\u63d0\u53d6(protein extraction)\u548c\u7d30\u80de\u5206\u96e2(cell fractionation)\u800c\u4eab\u8b7d\u5168\u7403\u3002\u6211\u5011\u958b\u767c\u4e86\u57fa\u65bc\u65cb\u8f49\u67f1(spin column-based)\u7684\u5275\u65b0\u6280\u8853\uff0c\u7528\u65bc\u86cb\u767d\u8cea\u7814\u7a76( protein research)\uff0c\u53ef\u7c21\u5316\u548c\u589e\u5f37\u6a23\u54c1\u88fd\u5099\u3002\u9019\u6703\u5e36\u4f86\u66f4\u9806\u66a2\u7684\u5de5\u4f5c\u6d41\u7a0b\u548c\u59cb\u7d42\u53ef\u9760\u7684\u7d50\u679c\uff0c\u5f9e\u800c\u5e36\u4f86\u5353\u8d8a\u7684\u6578\u64da\u8cea\u91cf\u3002\u9078\u64c7 Minute\u2122 \u8a66\u5291\u76d2\u9032\u884c\u86cb\u767d\u8cea\u63d0\u53d6(protein extraction)\u548c\u7d30\u80de\u5206\u7d1a\u5206\u96e2(cell fractionation)\u7684\u7814\u7a76\u4eba\u54e1\u53ef\u4ee5\u76f8\u4fe1\uff0c\u4ed6\u5011\u7684\u6a23\u54c1\u6e96\u78ba\u5730\u4ee3\u8868\u4e86\u6240\u7814\u7a76\u7684\u7d30\u80de\u6216\u7d44\u7e54\u7684\u81ea\u7136\u7279\u5fb5\u3002\u9019\u4e9b\u5957\u4ef6\u53ea\u9700\u8981\u4e00\u53f0\u53f0\u5f0f\u96e2\u5fc3\u6a5f\uff0c\u6700\u5927\u9650\u5ea6\u5730\u6e1b\u5c11\u5be6\u9a57\u8b8a\u91cf\u4e26\u78ba\u4fdd\u9ad8\u8cea\u91cf\u7684\u6750\u6599\u4ee5\u7372\u5f97\u66f4\u53ef\u9760\u7684\u6578\u64da\u3002Minute\u2122 \u65b9\u6cd5\u7684\u6709\u6548\u6027\u5f97\u5230\u4e86\u8d8a\u4f86\u8d8a\u591a journals\u7684\u652f\u6301 (Nature, Science, and Cell.)\u3002<\/p>\n<p><span style=\"font-size: 12pt; color: #ff0000;\"><b>The Minute\u2122 \u7684\u512a\u9ede\uff1a<\/b><\/span><br \/>\n\u2022 \u8d85\u5feb\uff08\u5feb\u81f3 1 \u5206\u9418\uff09<br \/>\n\u2022 \u6a23\u672c\u91cf\u5c0f<br \/>\n\u2022 \u6700\u5c0f\u5316\u4ea4\u53c9\u6c61\u67d3<br \/>\n\u2022 \u5167\u6e90\u6027\u57fa\u7dda\u4e0d\u8b8a<br \/>\n\u2022 \u6240\u6709\u6d3b\u9ad4\u548c\u7d44\u7e54\u985e\u578b<\/p>\n<p><span style=\"color: #ff0000;\"><strong><span style=\"font-size: 12pt;\">The Minute\u2122 \u61c9\u7528\uff1a<\/span><\/strong><\/span><br \/>\n\u2022 \u86cb\u767d\u8cea\u904b\u8f38\/\u6613\u4f4d<br \/>\n\u2022 \u86cb\u767d\u8cea-\u86cb\u767d\u8cea\u76f8\u4e92\u4f5c\u7528<br \/>\n\u2022 \u86cb\u767d\u8cea\u4fee\u98fe<br \/>\n\u2022 \u4fe1\u865f\u8f49\u5c0e<br \/>\n\u2022 sc\/snRNA-seq<\/p>\n<p><strong><span style=\"font-size: 12pt; color: #800000;\">Invent Minute\u2122 \u5fb9\u5e95\u6539\u8b8a\u86cb\u767d\u8cea\u6a23\u54c1\u88fd\u5099\u548c\u7d30\u80de\u5206\u7d1a\u5206\u96e2<\/span><\/strong><\/p>\n<div class=\"rte\">\n<p>\u81ea 2010 \u5e74\u6210\u7acb\u4ee5\u4f86\uff0c\u6211\u5011\u7684\u9996\u8981\u76ee\u6a19\u662f\u5229\u7528\u6211\u5011\u57fa\u65bc\u65cb\u8f49\u67f1\u7684\u5275\u65b0\u6280\u8853\u5fb9\u5e95\u6539\u8b8a\u5168\u7403\u86cb\u767d\u8cea\u63d0\u53d6(protein extraction)\u548c\u7d30\u80de\u5206\u96e2(cell fractionation)\u5e02\u5834\u3002\u6211\u5011\u7684\u76ee\u6a19\u662f\u8b93 Minute\u2122 \u8a66\u5291\u76d2\u6210\u70ba\u6a19\u6e96\u5be6\u9a57\u5ba4\u7a0b\u5e8f\uff0c\u53d6\u4ee3\u6240\u6709\u57fa\u65bc\u6eb6\u6db2\u7684\u6a23\u54c1\u88fd\u5099\u65b9\u6cd5\u3002\u901a\u904e\u5229\u7528\u9019\u4e9b\u5957\u4ef6\uff0c\u7814\u7a76\u4eba\u54e1\u53ea\u9700\u4e00\u53f0\u53f0\u5f0f\u96e2\u5fc3\u6a5f\u5373\u53ef\u7372\u5f97\u4e00\u81f4\u7684\u7d50\u679c\u4e26\u7372\u5f97\u5353\u8d8a\u7684\u6578\u64da\u8cea\u91cf\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/06\/5-Minute%E2%84%A2-%E5%9B%BA%E5%AE%9A%E5%92%8C%E5%8C%85%E5%9F%8B%E7%B5%84%E7%B9%94%E8%9B%8B%E7%99%BD%E8%B3%AA%E6%8F%90%E5%8F%96%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FFE-025.jpg\" width=\"491\" height=\"239\" \/><\/p>\n<p><span style=\"font-size: 12pt;\"><strong><span style=\"color: #800000;\">\u6539\u8b8a\u86cb\u767d\u8cea\u63d0\u53d6(Transforming protein extraction)<\/span><\/strong><\/span><\/p>\n<p>Minute\u2122 \u7e3d\u86cb\u767d\u63d0\u53d6\u8a66\u5291\u76d2\u63a1\u7528\u7c21\u55ae\u7684\u65cb\u8f49\u67f1( simple spin column )\uff0c\u53ef\u6709\u6548\u6d88\u9664\u7d30\u80de\u6216\u7d44\u7e54\u88c2\u89e3\u904e\u7a0b(lysis)\u4e2d\u7684\u975e\u7cfb\u7d71\u6027\u86cb\u767d\u8cea\u640d\u5931(non-systematic protein loss)\u3002\u9019\u4ee3\u8868\u8457\u76f8\u5c0d\u65bc\u50b3\u7d71\u7684\u57fa\u65bc\u89e3\u6c7a\u65b9\u6848\u7684\u65b9\u6cd5\uff0c\u4f8b\u5982 RIPA \u7de9\u885d\u6db2(RIPA buffer)\u548c\u8d85\u8072\u8655\u7406(sonication)\u7684\u986f\u8457\u9032\u6b65\uff0c\u9019\u4e9b\u65b9\u6cd5\u4ee5\u5176\u53ef\u8b8a\u6027\u548c\u7121\u6cd5\u63d0\u4f9b\u5168\u9762\u7684\u86cb\u767d\u8cea\u8b5c\u800c\u805e\u540d\u3002\u7528\u65bc\u7e3d\u86cb\u767d\u63d0\u53d6\u7684 Minute\u2122 \u8a66\u5291\u76d2\u8207\u4f86\u81ea\u5404\u7a2e\u751f\u7269\u9ad4\u548c\u7d44\u7e54\u985e\u578b\u7684\u5c0f\u6a23\u672c\u91cf\u517c\u5bb9\uff0c\u70ba\u7814\u7a76\u4eba\u54e1\u63d0\u4f9b\u4e86\u672a\u6539\u8b8a\u7684\u5167\u6e90\u86cb\u767d\u57fa\u7dda( endogenous proteins)\uff0c\u5f9e\u800c\u5be6\u73fe\u66f4\u7cbe\u78ba\u7684\u5b9a\u91cf\u548c\u5206\u6790\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/Invent-Biotechs-Minute-Kit-shows-a-more-complete-cell-lysis-compared-to-RIPA-buffer-in-which-a-pellet-remains-leading-to-an-alteration-of-the-protein-profile.jpg\" width=\"374\" height=\"481\" \/><\/p>\n<p>Invent Biotech&#8217;s Minute Kit shows a more complete cell lysis compared to RIPA buffer, in which a pellet remains, leading to an alteration of the protein profile.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/06\/3-Minute%E2%84%A2-%E5%9B%BA%E5%AE%9A%E5%92%8C%E5%8C%85%E5%9F%8B%E7%B5%84%E7%B9%94%E8%9B%8B%E7%99%BD%E8%B3%AA%E6%8F%90%E5%8F%96%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FFE-025.jpg\" width=\"488\" height=\"243\" \/><\/p>\n<p><strong><span style=\"font-size: 12pt; color: #800000;\">\u6539\u9032\u7d30\u80de\u5206\u7d1a\u5206\u96e2(Revamping cell fractionation)<\/span><\/strong><\/p>\n<p>\u7531\u65bc\u7f3a\u4e4f\u7c21\u55ae\u7684\u5be6\u9a57\u65b9\u6848\uff0c\u50b3\u7d71\u7684\u7d30\u80de\u5206\u7d1a\u5206\u96e2\u65b9\u6cd5\u9577\u671f\u4ee5\u4f86\u4e00\u76f4\u53d7\u5230\u7528\u6236\u504f\u5dee\u548c\u7d50\u679c\u4e0d\u4e00\u81f4\u7684\u5f71\u97ff\u3002 Minute\u2122 \u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2\u5fb9\u5e95\u6539\u8b8a\u4e86\u9019\u4e00\u904e\u7a0b\uff0c\u4f7f\u7814\u7a76\u4eba\u54e1\u80fd\u5920\u5728 90 \u5206\u9418\u5167\u7372\u5f97\u9ad8\u7d14\u5ea6\u7684\u7d30\u80de\u5668\u7fa4\uff0c\u4e26\u4e14\u4f7f\u7528\u6700\u5c11\u7684\u6d17\u6ecc\u5291\u3002\u901a\u904e\u63a1\u7528\u7c21\u55ae\u7684\u65cb\u8f49\u67f1\uff0c\u9019\u4e9b\u8a66\u5291\u76d2\u7121\u9700\u5747\u8cea\u5316(homogenization)\u3001\u5bc6\u5ea6\u68af\u5ea6\u5206\u96e2(density gradient separation)\u548c\u8d85\u901f\u96e2\u5fc3\u7b49\u50b3\u7d71\u6280\u8853( ultra-centrifugation)\uff0c\u5f9e\u800c\u6e1b\u5c11\u4e86\u5be6\u9a57\u8b8a\u91cf\u7684\u6578\u91cf\u3002\u9019\u53ef\u4ee5\u9632\u6b62\u7d30\u80de\u5668\u640d\u50b7( organelle damage)\u4e26\u7522\u751f\u66f4\u4e00\u81f4\u548c\u751f\u7406\u76f8\u95dc\u7684\u7d50\u679c\u3002\u6191\u85c9\u591a\u7a2e Minute\u2122 \u8a66\u5291\u76d2\u53ef\u6eff\u8db3\u4e0d\u540c\u7684\u7d30\u80de\u5206\u7d1a(cell fractionation)\u5206\u96e2\u8981\u6c42\uff0c\u6211\u5011\u6b63\u5728\u63a8\u52d5\u86cb\u767d\u8cea\u7d44\u7814\u7a76\u7684\u8f49\u578b\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/06\/2-Minute%E2%84%A2-%E5%9B%BA%E5%AE%9A%E5%92%8C%E5%8C%85%E5%9F%8B%E7%B5%84%E7%B9%94%E8%9B%8B%E7%99%BD%E8%B3%AA%E6%8F%90%E5%8F%96%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FFE-025.jpg\" width=\"480\" height=\"238\" \/><\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>\u652f\u6301\u6240\u6709\u7814\u7a76\u5b78\u79d1(Supporting all research disciplines)<\/strong><\/span><\/p>\n<p>Minute\u2122 \u8a66\u5291\u76d2\u65e8\u5728\u7121\u7e2b\u96c6\u6210\u5230\u73fe\u6709\u7684\u86cb\u767d\u8cea\u63d0\u53d6\u548c\u7d30\u80de\u5206\u7d1a\u5206\u96e2\u5de5\u4f5c\u6d41\u7a0b\u4e2d\uff0c\u63d0\u4f9b\u5feb\u901f\u4e14\u4e00\u81f4\u7684\u6027\u80fd\uff0c\u78ba\u4fdd\u53ef\u9760\u7684\u7d50\u679c\u3002\u7136\u800c\uff0c\u4e0d\u8981\u53ea\u76f8\u4fe1\u6211\u5011\u7684\u8a71\u2014\u2014\u6211\u5011\u7684\u7522\u54c1\u5df2\u5728\u79d1\u5b78\u6587\u737b\u4e2d\u88ab\u5ee3\u6cdb\u5f15\u7528\uff0c\u9019\u8868\u660e\u7814\u7a76\u754c\u5c0d\u7c21\u5316\u4e14\u53ef\u9760\u7684\u6a23\u54c1\u88fd\u5099\u65b9\u6cd5\u7684\u9700\u6c42\u4e0d\u65b7\u589e\u9577\u3002\u7acb\u5373\u5207\u63db\u5230 Minute\u2122\uff0c\u9ad4\u9a57\u6211\u5011\u6280\u8853\u7684\u512a\u52e2\uff0c\u7121\u8ad6\u60a8\u7684\u7814\u7a76\u9818\u57df\u5982\u4f55\u3002<\/p>\n<table style=\"height: 2673px;\" width=\"995\">\n<tbody>\n<tr>\n<td width=\"64\">SD-001\/SN-002<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Total Protein Extraction Kit\u00a0 for Animal Cultured Cells\/Tissues (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SC-003<\/td>\n<td>Minute <sup>TM<\/sup> Cytoplasmic and Nuclear Fractionation kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SN-006<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Detergent-Free Total Protein Extraction Kit for Animal Cells\/Tissues (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SM-005<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Plasma Membrane Protein Isolation Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SM-005-p<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Plasma Membrane Protein Isolation Kit for Plants (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">MP-007<\/td>\n<td width=\"667\">Minute<sup> TM <\/sup>Mitochondria Isolation Kit for Mammalian Cells and Tissues(50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">MM-038<\/td>\n<td>Minute<sup> TM <\/sup>Mitochondria Isolation Kit for Muscle Tissues\/Cultured Muscle Cells (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SD-008\/SN-009<\/td>\n<td width=\"667\">Minute <sup>TM <\/sup>Total Protein Extraction Kit for Plant Tissues (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SN-010<\/td>\n<td width=\"667\">Minute <sup>TM <\/sup>Detergent-Free Plant Protein Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">CP-011<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Chloroplast Isolation Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SB-004<\/td>\n<td width=\"667\">Minute<sup> TM <\/sup>Bacterial Total Protein Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SC-012<\/td>\n<td>Minute <sup>TM<\/sup> Single Cell Isolation Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">NE-013<\/td>\n<td width=\"667\">Minute<sup> TM<\/sup> Nuclear Envelope Protein Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">HP-014<\/td>\n<td width=\"667\">Minute<sup> TM<\/sup> Histon\/DNA Binding\u00a0 Protein Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">YT-015<\/td>\n<td width=\"667\">Minute\u2122 Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">YD-016<\/td>\n<td width=\"667\">Minute\u2122 Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">YM-017<\/td>\n<td>Minute\u2122 Yeast Mitochondria Enrichment Kit (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">MM-018<\/td>\n<td width=\"667\">Minute\u2122 Plant Microsomal Membrane Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PN-019<\/td>\n<td width=\"667\">Minute\u2122 Protein\/Nucleic Acid Extraction Kit from Gels (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">HD-021<\/td>\n<td width=\"667\">MinuteTM Protein Extraction Kit for Hair and Nails (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">AT-022<\/td>\n<td width=\"667\">MinuteTM Total Protein Extraction Kit for Adipose Tissues\/Cultured Adipocytes (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">AF-023<\/td>\n<td>MinuteTM Adipose Tissue Fractionation Kit (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">NI-024<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Detergent-Free Nuclei Isolation Kit (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">BN-020<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Single Nucleus Isolation Kit for Neuronal Tissues\/Cells (20 preps)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SN-047<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Single Nucleus Isolation Kit for Tissues\/Cells (20 preps)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">AN-029<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Nuclei and Cytosol Isolation Kit for Adipose Tissues (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">FE-025<\/td>\n<td>Minute<sup>TM<\/sup> Protein Extraction Kit for Fixed and Embedded Tissues (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">MS-026<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Total Protein Extraction Kit for Mass Spectrometry (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">EI-027<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Hi-Efficiency Exosome Isolation Reagent (20 ml) (Non-PEG)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">ED-028<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Endosome Isolation and Cell Fractionation Kit (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SE-030<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Hi-Efficiency Saliva Exosome Isolation Kit (50 Tests) (Non-PEG)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">CS-031<\/td>\n<td>Minute<sup>TM<\/sup> Cell Suspension Isolation from Fresh\/Frozen Tissues (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">NT-032<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Cytosolic and Nuclear Extraction Kit for Frozen\/Fresh Tissues (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">NM-033<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Detergent-Free Nuclear Matrix Protein Extraction Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">LY-034<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Lysosome Isolation Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">ER-035<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> High Fidelity ER Isolation Kit for Liver Tissue (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">ER-036<\/td>\n<td>Minute<sup>TM<\/sup> ER Enrichment Kit\u00a0 (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">GO-037<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Golgi Apparatus Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">LR-039<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Lipid Raft Isolation Kit for Mammalian Cells\/Tissues (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">LR-042<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Lipid Raft Isolation Kit for Mammalian Cells\/Tissues (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PT-040<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Cytosolic Proteasome Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PN-041<\/td>\n<td>Minute<sup>TM<\/sup> Nuclear Proteasome Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PF-045<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Plant Cytosolic and Nuclear Protein Isolation Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SV-043<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Native Protein Precipitation Kit from Saliva (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">ML-044<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Organic Solvent-Free Milk Lipid Depletion Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">RD-046<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Rubisco Depletion Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PR-048<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Plant ER Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PG-049<\/td>\n<td>Minute<sup>TM<\/sup> Plant Golgi Apparatus Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PE-050<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Plant Endosome Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PL-051<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Plant Lipid Raft Isolation Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SY-052<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Synaptosome Isolation Kit (50 preps) NEW!<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SA-01-SK<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Total Protein Extraction Kit for Skin Tissue (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SA-02-BT<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Total Protein Extraction Kit for Bone Tissue (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SA-03-BV<\/td>\n<td>Minute<sup>TM <\/sup>Total Protein Extraction Kit for Blood Vessels (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SA-04-TD<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Total Protein Extraction Kit for Tendons (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SA-05-IM<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Total Protein Extraction Kit for Intestines and 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ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-008<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> WB Blocking Solution (250 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-009<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Denaturing Protein Solubilization Reagent (10 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-010<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Non-Denatured Protein Solubilization Reagent (10 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-011<\/td>\n<td>Minute<sup>TM<\/sup> Protein Solubilization Reagent for MS (2 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-012<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> SDS-Remover (10 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-013<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Albumin Depletion Reagent for Plasma and Serum (20 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-014<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Anti-Clumping Nuclei Storage Buffer NEW!<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u5feb\u901f\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u7cfb\u5217-Rapid Protein Extraction Kits Minute\u2122\u86cb\u767d\u8403\u53d6\u5957 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":4646,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5889],"tags":[6233],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44437"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=44437"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44437\/revisions"}],"predecessor-version":[{"id":52349,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44437\/revisions\/52349"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/4646"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=44437"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=44437"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=44437"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}