{"id":44207,"date":"2023-07-20T12:32:32","date_gmt":"2023-07-20T04:32:32","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=44207"},"modified":"2023-07-24T14:55:09","modified_gmt":"2023-07-24T06:55:09","slug":"label-it-%e6%a0%b8%e9%85%b8%e6%a8%99%e8%a8%98%e8%a9%a6%e5%8a%91-label-it-nucleic-acid-labeling-reagents-mirus","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=44207","title":{"rendered":"Label IT\u00ae \u6838\u9178\u6a19\u8a18\u8a66\u5291 | Label IT\u00ae Nucleic Acid Labeling Reagents-Mirus"},"content":{"rendered":"<h1><span style=\"color: #993366; font-size: 14pt;\"><strong>Label IT\u00ae \u6838\u9178\u6a19\u8a18\u8a66\u5291 &#8211; <span style=\"color: #ff9900;\"><em>Label<\/em> IT\u00ae Nucleic Acid Labeling Reagents-Mirus<\/span><\/strong><\/span><\/h1>\n<p><span style=\"color: #33cccc; font-size: 12pt;\"><a style=\"color: #33cccc;\" href=\"https:\/\/biopioneer.com.tw\/?p=44207\"><em>Label<\/em>\u00a0IT<sup>\u00ae<\/sup>\u00a0Nucleic Acid Labeling Kit, Cy<sup>\u00ae<\/sup>5\u8ca8\u865fMR-MIR3725 (25ug)\u00a0<\/a><\/span><\/p>\n<p>Efficient, direct, non-enzymatic labeling of DNA and RNA<\/p>\n<p>Label IT\u00ae \u6838\u9178\u6a19\u5b9a\u6280\u8853(Label IT\u00ae Nucleic Acid Labeling Technology )\u901a\u904e\u55ae\u4e00\u6b65\u9a5f<strong><span style=\"color: #000000;\">(One-step Chemical Method)<\/span><\/strong>\uff0c\u6a19\u8a18\u87a2\u5149\u6216\u9175\u7d20\u5206\u5b50\u81f3\u6838\u9178\u6a23\u54c1( DNA \u6216 RNA \u5167\u7684\u9e7c\u57fa)\u3002\u8207\u5176\u4ed6\u975e\u653e\u5c04\u6027\u6a19\u5b9a\u6280\u8853\u4f8b\u5982\u96a8\u6a5f\u5f15\u767c( random priming)\u3001\u5207\u53e3\u5e73\u79fb(nick translation\uff09\u4e0d\u540c\u3002Label IT\u00ae Nucleic Acid\u6a19\u8a18\u8a66\u5291\u70ba\u5171\u50f9\u9375\u9023\u63a5 (<strong>Covalent Mechanism)<\/strong>\uff0c\u7121\u9700\u986f\u8457\u6539\u8b8a\u8d77\u59cb\u6838\u9178(starting nucleic acid)\u6216\u963b\u7919\u4e0b\u6e38\u96dc\u4ea4\u6027\u80fd(hybridization performance)\u3002Label IT\u00ae \u53cd\u61c9\u4e0d\u6703\u7834\u58de\u6838\u9178\u8d77\u59cb\u6750\u6599\uff0c\u56e0\u6b64 Label IT\u00ae\u6280\u8853\u53ef\u5ee3\u6cdb\u7528\u65bc\u9ad4\u5167\u3001\u9ad4\u5916\u548c\u5206\u5b50\u751f\u7269\u5b78\u61c9\u7528\u3002\u6a19\u5b9a\u7684\u67d3\u5291\u5e38\u898b\u7684\u5982CX-\u7f85\u4e39\u660e(CX-rhodamine)\u3001\u87a2\u5149\u7d20(fluorescein)\u3001MFP488\u3001\u5730\u9ad8\u8f9b(digoxin)\u3001\u751f\u7269\u7d20( biotin)\u3001Cy\u00ae3\u3001Cy\u00ae5\u3001\u4e8c\u785d\u57fa\u82ef\u915a (dinitrophenol , DNP) \u6216 TM-\u7f85\u4e39\u660e(TM-rhodamine)\uff0c\u7531\u65bc Label IT\u00ae \u53cd\u61c9\u4e0d\u6703\u7834\u58de\u6838\u9178\u8d77\u59cb\u6750\u6599\uff0c\u56e0\u6b64 Label IT\u00ae\u6280\u8853\u53ef\u5ee3\u6cdb\u7528\u65bc\u9ad4\u5167\u3001\u9ad4\u5916\u548c\u5206\u5b50\u751f\u7269\u5b78\u61c9\u7528\u3002\u4f9d\u6a19\u6e96\u64cd\u4f5c\u6d41\u7a0b\uff0c\u6a19\u5b9a\u5bc6\u5ea6\u6bcf 20-60 \u500b\u9e7c\u57fa\u5c0d\u6709\u4e00\u500b Label IT\u00ae \u6a19\u5b9a\u3002\u8a72\u6a19\u5b9a\u5bc6\u5ea6\u53ef\u61c9\u7528\u65bc\u5927\u591a\u6578\u5be6\u9a57; \u57fa\u56e0\u8868\u9054\u6216 RNAi \u7814\u7a76\u7684\u61c9\u7528\u3002\u5982\u679c\u9700\u8981\u66ff\u4ee3\u7684\u6a19\u5b9a\u5bc6\u5ea6(labeling density)\uff0c\u53ea\u9700\u589e\u52a0\u6216\u6e1b\u5c11\u6a19\u5b9a\u91cf\u53cd\u61c9\u4e2d\u52a0\u5165\u8a66\u5291\u6216\u8abf\u6574\u53cd\u61c9\u5b75\u80b2\u6642\u9593\u3002<span style=\"color: #ff9900;\"><a style=\"color: #ff9900;\" href=\"https:\/\/www.mirusbio.com\/tech-resources\/faqs\/label-it-faqs\">\u6e96\u78ba\u7684\u6a19\u5b9a\u5bc6\u5ea6\u8acb\u53c3\u95b1<\/a><\/span><\/p>\n<ul>\n<li>\u4efb\u4f55 DNA\u3001RNA \u6a21\u677f\u7686\u53ef\u9069\u7528<\/li>\n<li>\u591a\u7a2e\u87a2\u5149\u3001\u975e\u87a2\u5149\u6a19\u5b9a\u5206\u5b50\u53ef\u9078\u3002<\/li>\n<li>\u5316\u5b78\u6a19\u5b9a\u65b9\u5f0f\uff0c\u6d41\u7a0b\u7c21\u55ae\uff0c\u4e00\u5c0f\u6642\u5373\u53ef\u5b8c\u6210\u6a19\u5b9a\u53cd\u61c9\u3002<\/li>\n<li>\u4e0d\u6703\u7834\u58de\u6a19\u5b9a\u5c0d\u8c61 (\u6838\u9178\u5206\u5b50) \u7684\u7d50\u69cb\u5b8c\u6574\u6027\u3002<\/li>\n<li>\u6a19\u5b9a\u53cd\u61c9\u4e0d\u6703\u5f71\u97ff\u96dc\u4ea4 (hybridization) \u6548\u7387\u3002<\/li>\n<\/ul>\n<p><a href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/\u64cd\u4f5c\u624b\u518aLabel-IT\u00ae-Nucleic-Acid-Labeling-Kits.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/01\/%E7%B6%B2%E7%AB%99%E8%A6%8F%E6%A0%BC%E5%B0%8F%E5%9C%96_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-11-300x97.jpg\" alt=\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df 1_\u5de5\u4f5c\u5340\u57df 1\" width=\"135\" height=\"55\" \/><\/a><\/p>\n<p>Each Full Size Kit contains sufficient reagents to label 100 \u00b5g nucleic acid.<br \/>\nEach Trial Size Kit contains sufficient reagents to label 25 \u00b5g nucleic acid.<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/Label-IT%C2%AE-%E6%A0%B8%E9%85%B8%E6%A8%99%E8%A8%98%E8%A9%A6%E5%8A%91-Label-IT%C2%AE-Nucleic-Acid-Labeling-Reagents-Mirus.png\" \/><\/p>\n<p>Label IT\u00ae \u6838\u9178\u6a19\u5b9a\u8a66\u5291\u76d2\u3002 Label IT\u00ae \u5316\u5b78\u6a19\u5b9a\u8a66\u5291\u7531\u4e09\u500b\u5340\u57df\u7d44\u6210\uff1a\u6a19\u8a18\uff08\u87a2\u5149\u5718\u6216\u534a\u6297\u539f\uff09\uff08\u7da0\u8272\uff09\u3001\u4fc3\u9032\u8207\u6838\u9178\u975c\u96fb\u76f8\u4e92\u4f5c\u7528\u7684\u9023\u63a5\u57fa\u5718\uff08\u9ec3\u8272\uff09\u4ee5\u53ca\u5171\u50f9\u9023\u63a5 Label IT \u7684\u53cd\u61c9\u6027\u70f7\u57fa\u5316\u57fa\u5718\uff08\u85cd\u8272\uff09 \u00ae \u8a66\u5291\u53ef\u8207\u6838\u9178\u5167\u7684\u4efb\u4f55\u53cd\u61c9\u6027\u96dc\u539f\u5b50\u7d50\u5408\u3002 Label IT\u00ae \u8a66\u5291\u8207\u6838\u9178\u7684\u9023\u63a5\u4e0d\u6703\u6539\u8b8a\u6838\u9178\u7684\u7d50\u69cb\u6216\u5f71\u97ff\u4e0b\u6e38\u96dc\u4ea4\u6027\u80fd\uff0c\u56e0\u6b64\uff0c\u4f7f\u7528 Label IT\u00ae \u8a66\u5291\u6a19\u8a18\u7684\u6838\u9178\u53ef\u7528\u65bc\u7814\u7a76\u4eba\u54e1\u5b9a\u7fa9\u7684\u591a\u7a2e\u61c9\u7528\u4e2d\u3002<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/2-Label-IT%C2%AE-%E6%A0%B8%E9%85%B8%E6%A8%99%E8%A8%98%E8%A9%A6%E5%8A%91-Label-IT%C2%AE-Nucleic-Acid-Labeling-Reagents-Mirus.gif\" \/><\/p>\n<p><strong>Non-destructive Direct Labeling of Nucleic Acid.<\/strong> \u5c0f\u9f20\u7e3d RNA \u7528 Label IT\u00ae Cy\u00ae3 (B) \u6a19\u8a18\uff0c\u4e26\u5728 Agilent RNA 6000 Nano \u82af\u7247\u4e0a\u8207\u672a\u6a19\u8a18\u7684\u7e3d RNA (A) \u4e00\u8d77\u9032\u884c\u96fb\u6cf3\u548c\u5206\u6790\u3002\u5c0d\u65bc\u6a19\u5b9a\u548c\u672a\u6a19\u5b9a\u7684\u6a23\u54c1\uff0cRNA \u5b8c\u6574\u6027\u503c (RIN) \u7d04\u70ba 9.0\u3002<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/3-Label-IT%C2%AE-%E6%A0%B8%E9%85%B8%E6%A8%99%E8%A8%98%E8%A9%A6%E5%8A%91-Label-IT%C2%AE-Nucleic-Acid-Labeling-Reagents-Mirus.png\" \/><br \/>\n<strong>Labeling Density can be Easily Controlled with the\u00a0<em>Label<\/em>\u00a0IT\u00ae Reagents.<\/strong>\u00a0Plasmid DNA was labeled with increasing ratios (w:w) of\u00a0<em>Label<\/em>\u00a0IT\u00ae CX-Rhodamine to DNA. The extent of labeling was estimated, and normalized to the amount of recovered DNA (# labels\/100 base pairs), using two different criteria: fluorescence intensity of the attached rhodamine (excitation at 585 nm, emission at 610 nm), and visible absorbance at 585 nm. The significance of quenching, at the higher labeling ratios, becomes apparent in the fluorimetric estimations of labeling density.<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/4-Label-IT%C2%AE-%E6%A0%B8%E9%85%B8%E6%A8%99%E8%A8%98%E8%A9%A6%E5%8A%91-Label-IT%C2%AE-Nucleic-Acid-Labeling-Reagents-Mirus.png\" \/><\/p>\n<p><strong><em>Label<\/em>\u00a0IT\u00ae MFP488 Labeled Plasmid DNA is Less Sensitive to Photobleaching as Compared to\u00a0<em>Label<\/em>\u00a0IT\u00ae Fluorescein Labeled Plasmid DNA.<\/strong>\u00a0<strong>(A)<\/strong>\u00a0Plasmid DNA was \u00a0labeled with\u00a0<em>Label<\/em>\u00a0IT\u00ae MFP488 or\u00a0<em>Label<\/em>\u00a0IT\u00ae Fluorescein. \u00a0Purified DNA was transfected into HeLa cells using the\u00a0<em>Trans<\/em>IT\u00ae-LT1 Transfection Reagent at a 3:1 reagent-to-DNA ratio (vol:wt). Two hours post transfection labeled DNA was visualized using a 63X oil immersion objective on a Zeiss Axiovert Inverted Fluorescence microscope. Images were captured before and after a 2 minute photobleaching period from a FITC light source.\u00a0<strong>(B)<\/strong>\u00a0The ratio (X-axis) of the brightness of individual puncta was calculated by dividing the photobleached value for fluorescence brightness by the value obtained from non-photobleached values for four separate images using Image J software (NIH), the average and standard deviation are shown. Photobleaching was also measured after 5 minutes for\u00a0<em>Label<\/em>\u00a0IT-MFP488 labeled plasmid DNA and the signal was still 4-fold higher than\u00a0<em>Label<\/em>\u00a0IT\u00ae Fluorescein labeled plasmid DNA at 2 minutes (data not shown).<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/5-Label-IT%C2%AE-%E6%A0%B8%E9%85%B8%E6%A8%99%E8%A8%98%E8%A9%A6%E5%8A%91-Label-IT%C2%AE-Nucleic-Acid-Labeling-Reagents-Mirus.png\" \/><\/p>\n<p><strong><em>Label<\/em>\u00a0IT\u00ae MFP488 Labeled Plasmid DNA is More Resistant to the Effects of Low pH as Compared to\u00a0<em>Label<\/em>\u00a0IT\u00ae Fluorescein Labeled Plasmid DNA.<\/strong>\u00a0Plasmid DNA was labeled at a low density, 1 label per 65 bp, using\u00a0<em>Label<\/em>\u00a0IT\u00ae MFP488 or\u00a0<em>Label<\/em>\u00a0IT\u00ae Fluorescein Nucleic Acid Labeling Kits. Purified DNA was subsequently diluted in various pH buffers (50 mM HEPES, pH 8, 100 mM MES, pH 5.4, and 100 mM MES, pH 3). Fluorescence was measured using a Tecan Genios Fluorometer and normalized to the respective control measured at 50 mM HEPES, pH 8.<\/p>\n<table class=\"tb-order\" style=\"height: 703px;\" width=\"1027\">\n<thead>\n<tr>\n<th>Product Name<\/th>\n<th nowrap=\"nowrap\">Pack Size<\/th>\n<th>Cat. No.<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td><em>Label<\/em>\u00a0IT<sup>\u00ae<\/sup>\u00a0Nucleic Acid Labeling Kit, Biotin<\/td>\n<td style=\"text-align: left;\" align=\"right\" nowrap=\"nowrap\">\n<p>labels 25 \u00b5g nucleic acid<\/p>\n<hr \/>\n<p>labels 100 \u00b5g nucleic acid<\/td>\n<td nowrap=\"nowrap\">\n<p>MR-MIR3425<\/p>\n<hr \/>\n<p>MR-MIR3400<\/td>\n<\/tr>\n<tr>\n<td><em>Label<\/em>\u00a0IT<sup>\u00ae<\/sup>\u00a0Nucleic Acid Labeling Kit, CX-Rhodamine<\/td>\n<td style=\"text-align: left;\" align=\"right\" nowrap=\"nowrap\">\n<p>labels 25 \u00b5g nucleic acid<\/p>\n<hr \/>\n<p>labels 100 \u00b5g nucleic acid<\/td>\n<td nowrap=\"nowrap\">\n<p>MR-MIR3125<\/p>\n<hr \/>\n<p>MR-MIR3100<\/td>\n<\/tr>\n<tr>\n<td><em>Label<\/em>\u00a0IT<sup>\u00ae<\/sup>\u00a0Nucleic Acid Labeling Kit, Cy<sup>\u00ae<\/sup>3<\/td>\n<td style=\"text-align: left;\" align=\"right\" nowrap=\"nowrap\">\n<p>labels 25 \u00b5g nucleic acid<\/p>\n<hr \/>\n<p>labels 100 \u00b5g nucleic acid<\/td>\n<td nowrap=\"nowrap\">\n<p>MR-MIR3625<\/p>\n<hr \/>\n<p>MR-MIR3600<\/td>\n<\/tr>\n<tr>\n<td><em>Label<\/em>\u00a0IT<sup>\u00ae<\/sup>\u00a0Nucleic Acid Labeling Kit, Cy<sup>\u00ae<\/sup>5<\/td>\n<td style=\"text-align: left;\" align=\"right\" nowrap=\"nowrap\">\n<p>labels 25 \u00b5g nucleic acid<\/p>\n<hr \/>\n<p>labels 100 \u00b5g nucleic acid<\/td>\n<td nowrap=\"nowrap\">\n<p>MR-MIR3725<\/p>\n<hr \/>\n<p>MR-MIR3700<\/td>\n<\/tr>\n<tr>\n<td><em>Label<\/em>\u00a0IT<sup>\u00ae<\/sup>\u00a0Nucleic Acid Labeling Kit, Digoxin<\/td>\n<td style=\"text-align: left;\" align=\"right\" nowrap=\"nowrap\">\n<p>labels 25 \u00b5g nucleic acid<\/p>\n<hr \/>\n<p>labels 100 \u00b5g nucleic acid<\/td>\n<td nowrap=\"nowrap\">\n<p>MR-MIR3325<\/p>\n<hr \/>\n<p>MR-MIR3300<\/td>\n<\/tr>\n<tr>\n<td><em>Label<\/em>\u00a0IT<sup>\u00ae<\/sup>\u00a0Nucleic Acid Labeling Kit, DNP<\/td>\n<td style=\"text-align: left;\" align=\"right\" nowrap=\"nowrap\">\n<p>labels 25 \u00b5g nucleic acid<\/p>\n<hr \/>\n<p>labels 100 \u00b5g nucleic acid<\/td>\n<td nowrap=\"nowrap\">\n<p>MR-MIR3825<\/p>\n<hr \/>\n<p>MR-MIR3800<\/td>\n<\/tr>\n<tr>\n<td><em>Label<\/em>\u00a0IT<sup>\u00ae<\/sup>\u00a0Nucleic Acid Labeling Kit, Fluorescein<\/td>\n<td style=\"text-align: left;\" align=\"right\" nowrap=\"nowrap\">\n<p>labels 25 \u00b5g nucleic acid<\/p>\n<hr \/>\n<p>labels 100 \u00b5g nucleic acid<\/td>\n<td nowrap=\"nowrap\">\n<p>MR-MIR3225<\/p>\n<hr \/>\n<p>MR-MIR3200<\/td>\n<\/tr>\n<tr>\n<td><em>Label<\/em>\u00a0IT<sup>\u00ae<\/sup>\u00a0Nucleic Acid Labeling Kit, MFP488<\/td>\n<td style=\"text-align: left;\" align=\"right\" nowrap=\"nowrap\">\n<p>labels 25 \u00b5g nucleic acid<\/p>\n<hr \/>\n<p>labels 100 \u00b5g nucleic acid<\/td>\n<td nowrap=\"nowrap\">\n<p>MR-MIR7125<\/p>\n<hr \/>\n<p>MR-MIR7100<\/td>\n<\/tr>\n<tr>\n<td><em>Label<\/em>\u00a0IT<sup>\u00ae<\/sup>\u00a0Nucleic Acid Labeling Kit, TM-Rhodamine<\/td>\n<td style=\"text-align: left;\" align=\"right\" nowrap=\"nowrap\">\n<p>labels 25 \u00b5g nucleic acid<\/p>\n<hr \/>\n<p>labels 100 \u00b5g nucleic acid<\/td>\n<td nowrap=\"nowrap\">\n<p>MR-MIR4125<\/p>\n<hr \/>\n<p>MR-MIR4100<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Label IT\u00ae \u6838\u9178\u6a19\u8a18\u8a66\u5291 &#8211; Label IT\u00ae Nucleic Acid Labelin [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":44212,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5971],"tags":[5984],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44207"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=44207"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44207\/revisions"}],"predecessor-version":[{"id":44220,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44207\/revisions\/44220"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/44212"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=44207"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=44207"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=44207"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}