{"id":43590,"date":"2023-07-04T14:10:53","date_gmt":"2023-07-04T06:10:53","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=43590"},"modified":"2023-08-30T14:06:09","modified_gmt":"2023-08-30T06:06:09","slug":"%e5%b0%8f%e9%bc%a0%e7%9d%be%e9%85%ae-t-testosterone-%e6%aa%a2%e6%b8%acelisa%e8%a9%a6%e5%8a%91%e7%9b%92%ef%bc%88%e7%ab%b6%e7%88%ad%e6%b3%95%ef%bc%89-%e8%b2%a8%e8%99%9fck-bio-24579","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=43590","title":{"rendered":"\u5c0f\u9f20\u777e\u916e (T) (Testosterone) \u6aa2\u6e2cELISA\u8a66\u5291\u76d2\uff08\u7af6\u722d\u6cd5\uff09 \u8ca8\u865fCK-bio-24579"},"content":{"rendered":"

\u5c0f\u9f20\u777e\u916e (T) <\/span>(Testosterone) \u6aa2\u6e2cELISA\u8a66\u5291\u76d2\uff08\u7af6\u722d\u6cd5\uff09 \u8ca8\u865fCK-bio-24579<\/strong><\/span><\/a><\/span><\/p>\n

Mouse Testosterone(T) ELISA Kit (competitive method)<\/span><\/p>\n

\u5075\u6e2c\u7bc4\u570d:1nmol\/L -32nmol\/L , \u6700\u4f4e\u9748\u654f\u5ea60.1nmol\/L<\/span><\/p>\n

\u777e\u916e(Testosterone)\u662f\u5206\u6ccc\u5230\u8840\u6db2\u4e2d\u6700\u91cd\u8981\u7684\u96c4\u6fc0\u7d20\u3002\u5728\u7537\u6027\u4e2d\uff0c\u777e\u916e\u4e3b\u8981\u7531\u777e\u4e38\u7684\u9593\u8cea\u7d30\u80de\u5206\u6ccc\u3002\u5728\u5973\u6027\u7d04 50% \u7684\u5faa\u74b0\u777e\u916e\u4f86\u81ea\u96c4\u70ef\u4e8c\u916e\u7684\u5916\u5468\u8f49\u5316\uff0c\u7d0425% \u4f86\u81ea\u5375\u5de2\u548c\u7d045%\u4f86\u81ea\u814e\u4e0a\u817a\u3002\u5728\u5973\u6027\u4e2d\uff0c\u9ad8\u6c34\u5e73\u7684\u777e\u916e\u4e00\u822c\u898b\u65bc\u591a\u6bdb\u75c7\u548c\u7537\u6027\u5316\u3001\u591a\u56ca\u5375\u5de2(polycystic ovaries)\u3001\u5375\u5de2\u816b\u7624( ovarian tumors)\u3001\u814e\u4e0a\u817a\u816b\u7624( adrenal tumors )\u548c\u814e\u4e0a\u817a\u589e\u751f(adrenal hyperplasia)\u3002\u5728\u7537\u6027\u4e2d\uff0c\u9ad8\u6c34\u5e73\u7684\u777e\u916e(testosterone)\u8207\u4e0b\u4e18\u8166\u5782\u9ad4\u75be\u75c5(hypothalamic pituitary )\u3001\u777e\u4e38\u816b\u7624( testicular tumors)\u3001\u5148\u5929\u6027\u814e\u4e0a\u817a\u589e\u751f(congenital adrenal hyperplasia)\u548c\u524d\u5217\u817a\u764c(prostate cancer)\u6709\u95dc\u3002\u60a3\u6709\u4ee5\u4e0b\u75be\u75c5\u7684\u60a3\u8005\u53ef\u80fd\u6703\u51fa\u73fe\u777e\u916e\u6c34\u5e73\u4f4e\u4e0b\u7684\u60c5\u6cc1\uff1a\u5782\u9ad4\u529f\u80fd\u6e1b\u9000\u75c7\u3001\u514b\u6c0f\u7d9c\u5408\u5fb5\u3001\u777e\u4e38\u5973\u6027\u5316\u3001\u6216\u4e73\u623f\u5207\u9664\u8853\u548c\u96b1\u777e\u3001\u9176\u7f3a\u9677\u548c\u4e00\u4e9b\u81ea\u8eab\u514d\u75ab\u6027\u75be\u75c5\u3002<\/p>\n

\u5c0f\u9f20\u777e\u916e (Testosterone) \u6aa2\u6e2c\u9176\u806f\u514d\u75ab\u5438\u9644\u6e2c\u5b9a\uff08ELISA\uff09\u8a66\u5291\u76d2\u662f\u4e00\u7a2e\u9ad4\u5916\u6e2c\u5b9a\u5c0f\u9f20\u777e\u916e (T) (Testosterone) \u7684\u5b9a\u91cf\u6e2c\u5b9a\u3002 CoonKoon\u7684\u5c0f\u9f20\u777e\u916e (T) (Testosterone) ELISA\u8a66\u5291\u76d2\u662f\u4e00\u7a2e\u7af6\u722d\u6027\u9176\u514d\u75ab\u6e2c\u5b9a\u8a66\u5291\u76d2(competitive method)\u3002\u5c07 Standard \u548c Sample \u6dfb\u52a0\u5230\u9810\u5148\u5305\u88ab\u76ee\u7684\u6297\u9ad4\u7684\u5b54\u4e2d\uff0c\u7136\u5f8c\u52a0\u5165\u93c8\u9ef4\u89aa\u548c\u7d20 HRP \u5f62\u6210\u514d\u75ab\u8907\u5408\u7269\uff0c\u5b75\u80b2\uff0c\u901a\u904e\u5b75\u80b2\u548c\u6d17\u6ecc\uff0c\u53bb\u9664\u672a\u7d50\u5408\u7684\u9176\uff0c\u7136\u5f8c\u52a0\u5165\u5e95\u7269A\u548cB\uff0c\u5247\u6eb6\u6db2\u5728\u9178\u7684\u4f5c\u7528\u4e0b\u6703\u8b8a\u6210\u85cd\u8272\uff0c\u6700\u5f8c\u8b8a\u6210\u9ec3\u8272\u3002\u984f\u8272\u6df1\u6dfa\u8207\u6aa2\u6e2c\u5c0f\u9f20\u777e\u916e (T) (Testosterone) \u7684\u6fc3\u5ea6\u5448\u8ca0\u76f8\u95dc\u30021nmol\/L -32nmol\/L , \u6700\u4f4e\u9748\u654f\u5ea60.1nmol\/L\u3002\u6ce8\u610f\u4e8b\u9805\uff1a 5\u5929\u5167\u4f7f\u7528\u7684\u6a23\u54c1\u53ef\u57282-8\u2103\u4fdd\u5b58\uff0c\u5426\u5247\u6a23\u54c1\u5fc5\u9808\u5728-20\u2103\uff08\u22641\u500b\u6708\uff09\u6216-80\u2103\uff08\u22642\u500b\u6708\uff09\u4fdd\u5b58\uff0c\u4ee5\u514d\u5931\u53bb\u751f\u7269\u6d3b\u6027\uff0c\u907f\u514d\u6c61\u67d3\u53ca\u53cd\u8986\u51b7\u51cd\u3002<\/p>\n

\u5373\u7528\u578b\u6a19\u6e96\u54c1\u542b\u67096\u74f6\u6fc3\u5ea6\uff0c\u4e0d\u9700\u81ea\u884c\u914d\u88fd\u3002\u6bcf\u74f6 0.5ml (\u6fc3\u5ea6\u4f9d\u4e0d\u540c\u7522\u54c1\u6709\u6240\u4e0d\u540c)\u00a0<\/span><\/strong><\/p>\n

Double speed Elisa \u8a66\u5291\u76d2\u5728 90 -120\u5206\u9418\u5167\u5373\u53ef\u5feb\u901f\u7372\u5f97\u5be6\u9a57\u7d50\u679c\uff1b\u00a0\u7af6\u722d\u6cd5(competitive method)2-3\u5c0f\u6642\u3002\u4fdd\u6301\u8457\u5353\u8d8a\u7684\u5075\u6e2c\u5c08\u4e00\u6027\u8207\u9748\u654f\u5ea6\u3002<\/span><\/strong><\/p>\n

For the quantitative determination of Mouse Testosterone(T)\u00a0concentrations in serum, plasma, saliva, urine, tissue homogenate, cell culture supernates,body fluid and other biological fluids.<\/p>\n

\"\"<\/p>\n

\u7522\u54c1<\/strong>\u63cf\u8ff0\uff1a<\/strong><\/span>\u672c\u8a66\u5291\u76d2\u63a1\u7528\u7af6\u722d\u6cd5(competitive method)<\/span>\u6aa2\u6e2c\u6a23\u54c1\u4e2d\u6aa2\u6e2c\u5c0f\u9f20\u777e\u916e (T) (Testosterone)\u7684\u542b\u91cf\u3002\u5c07 Standard \u548c Sample \u6dfb\u52a0\u5230\u9810\u5148\u5305\u88ab\u76ee\u7684\u6297\u9ad4\u7684\u5b54\u4e2d\uff0c\u7136\u5f8c\u52a0\u5165\u93c8\u9ef4\u89aa\u548c\u7d20 HRP \u5f62\u6210\u514d\u75ab\u8907\u5408\u7269\uff0c\u5b75\u80b2\uff0c\u901a\u904e\u5b75\u80b2\u548c\u6d17\u6ecc\uff0c\u53bb\u9664\u672a\u7d50\u5408\u7684\u9176\uff0c\u7136\u5f8c\u52a0\u5165\u5e95\u7269A\u548cB\uff0c\u5247\u6eb6\u6db2\u5728\u9178\u7684\u4f5c\u7528\u4e0b\u6703\u8b8a\u6210\u85cd\u8272\uff0c\u6700\u5f8c\u8b8a\u6210\u9ec3\u8272\u3002\u984f\u8272\u6df1\u6dfa\u8207\u6aa2\u6e2c\u5c0f\u9f20\u777e\u916e (T) (Testosterone) \u7684\u6fc3\u5ea6\u5448\u8ca0\u76f8\u95dc\u3002\u9748\u654f\u5ea6\u7bc4\u570d1nmol\/L -32nmol\/L, \u6700\u4f4e\u9748\u654f\u5ea60.1nmol\/L\u3002\u53ef\u61c9\u7528\u65bc\u4ee5\u4e0b\u6a23\u54c1\u4f86\u6e90:\u8840\u6e05(Serum)\u3001\u8840\u6f3f(plasma)\u3001\u5c3f\u6db2(urine)\u3001\u553e\u6db2(saliva)\u3001\u7d44\u7e54\u52fb\u6f3f(tissue homogenates)\u3001\u7d30\u80de\u88c2\u89e3\u7269(cell lysates)\u3001\u7d30\u80de\u57f9\u990a\u7269\u4e0a\u6e05\u6db2(cell culture supernates)\u548c\u5176\u4ed6\u751f\u7269\u9ad4(biological fluids)\u6db2\u3002<\/p>\n

\u5c0f\u9f20\u777e\u916e (T) (Testosterone)ELISA \u8a66\u5291\u76d2<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Name<\/td>\nMouse Testosterone(T) ELISA Kit (competitive method)<\/span><\/td>\n<\/tr>\n
Cat No.<\/td>\nCK-bio-24579<\/td>\n<\/tr>\n
Specification<\/td>\n48T\/96T\/5*96T\/10*96T<\/td>\n<\/tr>\n
Detection range<\/td>\n1nmol\/L -32nmol\/L<\/td>\n<\/tr>\n
Detection limit<\/td>\n0.1nmol\/L<\/td>\n<\/tr>\n
Test principle<\/td>\nQuantitative<\/td>\n<\/tr>\n
Test method<\/td>\ncompetitive assay \u7af6\u722d\u6cd5(competitive method)2-3\u5c0f\u6642<\/span><\/td>\n<\/tr>\n
Storage<\/td>\nThe unopened kit should be stored at 2 – 8\u00b0C. After opening, please store refer to the manual.<\/td>\n<\/tr>\n
Sample type<\/td>\nSerum, plasma, cell culture supernatants, body fluid and tissue homogenate.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\"\"<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

Sample collection and storages<\/strong><\/span><\/p>\n

1.Serum:<\/strong> Allow the serum to clot for 10-20 minutes at room temperature. Centrifuge (at 2000-3000 RPM) for 20 minutes. Collect the supernatants carefully. When sediments occurred during storage, centrifugation should be performed again.<\/p>\n

2.Blood plasma<\/strong>: In accordance with the requirements of sample collection, EDTA or sodium citrate should be used as anti coagulation. Add EDTA or sodium citrate and mix them for 10-20 minutes. Centrifuge (at 2000-3000 RPM) for approximately 20 minutes. Collect the supernatants carefully. When sediments occurred during storage, centrifugation should be performed again.<\/p>\n

3.Urine<\/strong>: Collect by sterile tube. Centrifuge (at 2000-3000 RPM) for approximately 20 minutes. Collect the supernatants carefully. When sediments occurred during storage, centrifugation should be performed again. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.<\/p>\n

4.Cell culture supernatant<\/strong>: Collect by sterile tubes when examining secrete components. Centrifuge (at 2000-3000 RPM) for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (PH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million\/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge (at 2000-3000 RPM) for approximately 20 minutes. Collect the supernatants carefully. When sediments occurred during storage, centrifugation should be performed again.<\/p>\n

5.Tissue sample<\/strong>: Incise sample and weigh up. Add a certain amount of PBS (PH 7.4). Freeze with liquid nitrogen immediately for later use. Thaw the sample and keep it at 2-8\u2103. Add a certain amount of PBS (PH 7.4) and then homogenize the sample thoroughly by hand or homogenizer. Centrifuge (at 2000-3000 RPM) for approximately 20 minutes. Collect the supernatants carefully. Aliquot and keep one for examination and freeze the others for later use.<\/p>\n

6.saliva\u00a0sample:\u00a0<\/strong>Centrifuge samples for 20 minutes at 1000\u00d7g at 2-8\u00b0C. Collect supernatant and carry out the assay immediately.<\/p>\n

Note: <\/em><\/strong>1.Samples to be used within 5 days may be stored at 2-8\u2103, otherwise samples must be stored at -20\u2103 (\u22641month) or -80\u2103 (\u22642 months) to avoid loss of bioactivity and avoid contamination.<\/p>\n

2.Sample hemolysis will influence the result, so the samples should be centrifuged adequately and no hemolysis or granule was allowed.<\/p>\n

3.When performing the assay, bring samples to room temperature.<\/p>\n

Samples containing NaN3 can\u2019t be tested as it inhibits the activity of Horse Radish Peroxidase (HRP).<\/p>\n

4.After collecting the sample, extraction should be immediately carried out in accordance with related documents. After extraction, experiment should be conducted immediately as well. Otherwise, keep the sample at -20\u2103. Avoid repeated freeze-thaw cycles.<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716<\/p>\n

Coon-koon\u53f0\u7063\u4ee3\u7406\u5546-BRAND\/AGENCY\/\u4ee3\u7406\u54c1\u724c-Coon-koon<\/span><\/p>\n

http:\/\/www.coonkoonbio.com\/<\/a><\/p>\n

Shanghai Coon Koon Biotech Co.,Ltd\u662f\u4e00\u5bb6\u5c08\u696d\u5f9e\u4e8b\u751f\u7269\u88fd\u54c1\u7814\u767c\u3001\u751f\u7522\u548c\u7d93\u71df\u7684\u7d9c\u5408\u6027\u751f\u7269\u516c\u53f8\uff01 \u7522\u54c1\u9060\u92b7\u591a\u500b\u570b\u5bb6Coon Koon Bio\u4e3b\u8981\u505aELISA\u8a66\u5291\u76d2\u3001\u751f\u5316\u8a66\u5291\u3001\u6297\u9ad4\u7b49\u4e0a\u842c\u7a2e\u79d1\u7814\u7522\u54c1\uff01\u6d89\u53ca\u5206\u5b50\u751f\u7269\u5b78\u3001\u7d30\u80de\u751f\u7269\u5b78\u3001\u514d\u75ab\u5b78\u7b49\u5404\u500b\u9818\u57df\uff01\u5805\u6301\u201c\u4ee5\u8cea\u91cf\u70ba\u524d\u63d0\uff0c\u4ee5\u5ba2\u6236\u6eff\u610f\u70ba\u5b97\u65e8\u201d\uff1b\u78ba\u7acb\u201c\u4ee5\u5ba2\u6236\u9700\u6c42\u70ba\u5c0e\u5411\uff0c\u4ee5\u5e02\u5834\u9700\u6c42\u70ba\u5c0e\u5411\uff0c\u4e0d\u65b7\u958b\u767c\u512a\u8cea\u65b0\u7522\u54c1\uff0c\u63d0\u4f9b\u5ba2\u6236\u6eff\u610f\u3002<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Products Name<\/td>\nItem No.<\/td>\n<\/tr>\n
General Low Density Lipoprotein (LDL ) ELISA Kit<\/a><\/td>\nCK-bio-20392<\/td>\n<\/tr>\n
General growth hormone (GH) ELISA Kit<\/a><\/td>\nCK-bio-24744<\/td>\n<\/tr>\n
General High Density Lipoprotein (HDL ) ELISA Kit<\/a><\/td>\nCK-bio-26360<\/td>\n<\/tr>\n
Vitamin K(VK) ELISA Kit<\/a><\/td>\nCK-bio-13972<\/td>\n<\/tr>\n
Vitamin E(VE) ELISA Kit<\/a><\/td>\nCK-bio-19015<\/td>\n<\/tr>\n
Vitamin C(VC) ELISA Kit<\/a><\/td>\nCK-bio-18526<\/td>\n<\/tr>\n
Vitamin A(VA) ELISA Kit<\/a><\/td>\nCK-bio-15517<\/td>\n<\/tr>\n
Vesicular Glutamate Transporter 2 (VGLUT2) ELISA Kit<\/a><\/td>\nCK-bio-20679<\/td>\n<\/tr>\n
Vesicular Glutamate Transporter 1 (VGLUT1) ELISA Kit<\/a><\/td>\nCK-bio-20678<\/td>\n<\/tr>\n
vanillylmandelic acid(VMA) ELISA Kit<\/a><\/td>\nCK-bio-20812<\/td>\n<\/tr>\n
Tetracyclines(TCs) ELISA Kit<\/a><\/td>\nCK-bio-20775<\/td>\n<\/tr>\n
Glutamic Acid(Glu) ELISA Kit<\/a><\/td>\nCK-bio-20610<\/td>\n<\/tr>\n
Glucagon(GC) ELISA Kit<\/a><\/td>\nCK-bio-20678<\/td>\n<\/tr>\n
Aflatoxin M1(Aflatoxin M1) ELISA Kit<\/a><\/td>\nCK-bio-20774<\/td>\n<\/tr>\n
Thioguanine Nucleotide(6 TGN) ELISA Kit<\/a><\/td>\nCK-bio-20775<\/td>\n<\/tr>\n
6 Methyl Mercaptopurine(6 MMP) ELISA Kit<\/a><\/td>\nCK-bio-20776<\/td>\n<\/tr>\n
5-Aminolevulinate Synthase, Nonspecific, Mitochondrial(ALAS1) ELISA Kit<\/a><\/td>\nCK-bio-20813<\/td>\n<\/tr>\n
General \u03b1-glutathione S-transferases (\u03b1-GST) ELISA Kit<\/a><\/td>\nCK-bio-14048<\/td>\n<\/tr>\n
General Total Antioxidant Capacity (T-AOC) ELISA Kit<\/a><\/td>\nCK-bio-23742<\/td>\n<\/tr>\n
General Thiobarbituric Acid Reactive Substance (TBARS) ELISA Kit<\/a><\/td>\nCK-bio-23660<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u5c0f\u9f20\u777e\u916e (T) (Testosterone) \u6aa2\u6e2cELISA\u8a66\u5291\u76d2\uff08\u7af6\u722d\u6cd5\uff09 \u8ca8\u865fCK-bio-24579 […]<\/p>\n","protected":false},"author":3,"featured_media":38576,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5263],"tags":[5891],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/43590"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=43590"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/43590\/revisions"}],"predecessor-version":[{"id":45481,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/43590\/revisions\/45481"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/38576"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=43590"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=43590"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=43590"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}