{"id":43434,"date":"2023-06-27T15:14:06","date_gmt":"2023-06-27T07:14:06","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=43434"},"modified":"2023-07-04T16:03:21","modified_gmt":"2023-07-04T08:03:21","slug":"%e6%aa%b8%e6%aa%ac%e9%85%b8%e9%b9%bd-citrate%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-bioassay-systems-citrate-assay-kit-%e8%b2%a8%e8%99%9fecit-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=43434","title":{"rendered":"\u6ab8\u6aac\u9178\u9e7d( Citrate)\u6aa2\u6e2c\u8a66\u5291\u76d2 | BioAssay Systems&#8217; Citrate Assay Kit \u8ca8\u865fECIT-100"},"content":{"rendered":"<p><strong><span style=\"color: #33cccc; font-size: 12pt;\"><a style=\"color: #33cccc;\" href=\"https:\/\/biopioneer.com.tw\/?p=43434\">\u6ab8\u6aac\u9178\u9e7d( Citrate)\u6aa2\u6e2c\u8a66\u5291\u76d2(BioAssay Systems&#8217; Citrate Assay Kit) BioAssay Systems \u8ca8\u865fECIT-100<\/a><\/span><\/strong><\/p>\n<p><span style=\"color: #999999;\">\u53ef\u6aa2\u6e2c\u7dda\u6027\u6aa2\u6e2c\u7bc4\u570d\uff1a\u6ab8\u6aac\u9178\u9e7d\u70ba 4 \u81f3 400 \u00b5M\u542b\u91cf<\/span><\/p>\n<p>CITRATE \u662f\u6ab8\u6aac\u9178\u5faa\u74b0(citric acid cycle )\u7684\u4e2d\u9593\u9ad4\uff0c\u53c3\u8207\u8102\u80aa\u9178\u5408\u6210(fatty acid synthesis)\u3002 BioAssay Systems \u7684\u6ab8\u6aac\u9178\u9e7d\u6aa2\u6e2c\u8a66\u5291\u76d2(BioAssay Systems&#8217; Citrate Assay Kit)\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u4e14\u81ea\u52d5\u5316\u7684\u7a0b\u5e8f\u4f86\u6e2c\u91cf\u6ab8\u6aac\u9178\u9e7d\u6fc3\u5ea6(citrate concentration)\u3002\u6ab8\u6aac\u9178\u9e7d( Citrate)\u8f49\u5316\u70ba\u4e19\u916e\u9178\u9e7d( pyruvate)\uff0c\u7136\u5f8c\u4e19\u916e\u9178\u9e7d\u88ab\u6c27\u5316\uff0c\u67d3\u6599\u8f49\u5316\u70ba\u6709\u8272\u548c\u87a2\u5149\u5f62\u5f0f( fluorescent form)\u3002 570 nm \u8655\u7684\u984f\u8272\u5f37\u5ea6\u6216 \u03bbex\/em = 530\/585 nm \u8655\u7684\u87a2\u5149\u5f37\u5ea6\u8207\u6a23\u54c1\u4e2d\u6ab8\u6aac\u9178\u9e7d\u7684\u6fc3\u5ea6\u6210\u6b63\u6bd4\u3002<\/p>\n<p><strong><span style=\"color: #800000; font-size: 12pt;\">\u4e3b\u8981\u7279\u9ede (KEY FEATURES)<\/span><\/strong><\/p>\n<p>\u5feb\u901f\u3001\u9748\u654f\u3002\u7dda\u6027\u6aa2\u6e2c\u7bc4\u570d\uff1a\u7528\u65bc\u6bd4\u8272\u6e2c\u5b9a\u7684\u6ab8\u6aac\u9178\u9e7d\u70ba 4 \u81f3 400 \u00b5M\uff0c\u7528\u65bc\u87a2\u5149\u6e2c\u5b9a\u7684\u6ab8\u6aac\u9178\u9e7d\u70ba 0.5 \u81f3 40 \u00b5M\u3002\u65b9\u4fbf\u3001\u9ad8\u901a\u91cf\u3002\u5747\u8cea(Homogeneous)\u201c\u6df7\u5408-\u5b75\u80b2\u6e2c\u91cf(mix-incubatemeasure)\u201d\u578b\u6e2c\u5b9a\u3002\u53ef\u5728 HTS \u6db2\u9ad4\u8655\u7406\u7cfb\u7d71(HTS liquid handling systems)\u4e0a\u8f15\u9b06\u5be6\u73fe\u81ea\u52d5\u5316\uff0c\u6bcf\u5929\u8655\u7406\u6578\u5343\u500b\u6a23\u54c1\u3002\u61c9\u7528 \u751f\u7269\u6a23\u54c1\uff08\u4f8b\u5982\u8840\u6f3f\u3001\u8840\u6e05\u3001\u5c3f\u6db2\u3001\u7d44\u7e54\u548c\u57f9\u990a\u57fa\uff09\u4e2d\u7684\u6ab8\u6aac\u9178\u9e7d(Citrate)\u6e2c\u5b9a\u3002<\/p>\n<p><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\">\u6ce8\u610f\u4e8b\u9805<\/span><\/strong><\/span><span style=\"font-size: 12pt; color: #800000;\">Sample Preparation<strong>:<\/strong><\/span><\/p>\n<p>Tissue or cell samples (2 x10<sup>6<\/sup>) can be homogenized in 100 \u03bcL PBS. Centrifuge at 14,000 rpm for 5 min. Use clear supernatant for assay. If planning to measure citrate in culture media, avoid media with high pyruvate concentrations (e.g. DMEM, L-15, F12, etc.).<\/p>\n<p>Serum and plasma samples should be deproteinated using a 10 kDa spin filter (e.g. Amicon Ultra-0.5). Alternatively, untreated serum and plasma can measured directly if an internal standard is used.<\/p>\n<p>Urine samples should be diluted at least 5-fold and an internal standard should be used.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/03\/%E7%94%A2%E5%93%81%E7%89%B9%E9%BB%9E_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1-300x120.jpg\" alt=\"\u7522\u54c1\u7279\u9ede_\u5de5\u4f5c\u5340\u57df 1\" width=\"145\" height=\"59\" \/><\/p>\n<h4><span style=\"font-size: 12pt; color: #800000;\">Application<\/span><\/h4>\n<ul>\n<li>For quantitative determination of citrate or citric acid and evaluation of drug effects on citrate metabolism.<\/li>\n<\/ul>\n<h4><span style=\"font-size: 12pt; color: #800000;\">Key Features<\/span><\/h4>\n<ul>\n<li>Fast and sensitive. Linear detection range: 4 to 400 \u00b5M citrate for colorimetric assays and 0.5 to 40 \u00b5M for fluorimetric assays.<\/li>\n<li>Convenient and high-throughput. Homogeneous \u201cmix-incubate-measure\u201d type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.<\/li>\n<\/ul>\n<h4><span style=\"font-size: 12pt; color: #800000;\">Method<\/span><\/h4>\n<ul>\n<li>OD570nm, or FL530\/585nm<\/li>\n<\/ul>\n<h4><span style=\"font-size: 12pt; color: #800000;\">Samples<\/span><\/h4>\n<ul>\n<li>Serum, plasma, agriculture etc<\/li>\n<\/ul>\n<h4><span style=\"font-size: 12pt; color: #800000;\">Species<\/span><\/h4>\n<ul>\n<li>All<\/li>\n<\/ul>\n<h4><span style=\"font-size: 12pt; color: #800000;\">Procedure<\/span><\/h4>\n<ul>\n<li>20 min<\/li>\n<\/ul>\n<h4><span style=\"font-size: 12pt; color: #800000;\">Size<\/span><\/h4>\n<ul>\n<li>100 tests<\/li>\n<\/ul>\n<h4><span style=\"font-size: 12pt; color: #800000;\">Detection Limit<\/span><\/h4>\n<ul>\n<li>OD, FL: 4, 0.5 \u00b5M<\/li>\n<\/ul>\n<h4><span style=\"font-size: 12pt; color: #800000;\">Shelf Life<\/span><\/h4>\n<ul>\n<li>6 months<\/li>\n<\/ul>\n<h4><span style=\"font-size: 12pt; color: #800000;\">More Details<\/span><\/h4>\n<ul>\n<li><i>CITRATE\u00a0<\/i>is an intermediate in the citric acid cycle and is involved in fatty acid synthesis. BioAssay Systems&#8217; Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at \u03bbex\/em = 530\/585 nm is directly proportional to the citrate concentration in the sample.<\/li>\n<\/ul>\n<h2><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\"><a style=\"color: #800000;\" href=\"http:\/\/www.bioassaysys.com\/\">BioAssay Systems \u54c1\u724c\u4ecb\u7d39<\/a><\/span><\/strong><\/span><\/h2>\n<p>BioAssay Systems \u516c\u53f8\u7814\u88fd\u958b\u767c\u548c\u92b7\u552e\u5275\u65b0(innovative)\u9ad8\u901a\u91cf\u7684\u6aa2\u6e2c\u8a66\u5291\u7d44(high-throughput assay solutions)\uff0c\u4ee5\u6eff\u8db3\u4e0d\u65b7\u589e\u52a0\u7684\u9700\u6c42\u7684\u751f\u547d\u79d1\u5b78\u884c\u696d(life sciences industry)\u3002BioAssay Systems \u5c08\u6ce8\u65bc\u5b89\u5168\uff0c\u7121\u653e\u5c04\u6027\u7b49\u683c\u5f0f\u7684\u5438\u6536\u5149\uff0c\u87a2\u5149\u548c\u767c\u5149\u6aa2\u6e2c\u6280\u8853\u3002BioAssay Systems \u5728\u73fe\u4ee3\u85e5\u7269\u767c\u73fe\u65b9\u9762\u64c1\u6709 50 \u591a\u5e74\u7684\u8c50\u5bcc\u7d93\u9a57\u3002\u5728\u904e\u53bb\u7684 18 \u5e74\u4e2d\uff0c\u958b\u767c 200 \u591a\u7a2e\u7528\u65bc\u85e5\u7269\u767c\u73fe\u548c\u751f\u547d\u79d1\u5b78\u7814\u7a76\u7684\u6aa2\u6e2c\u8a66\u5291\u76d2\u3002\u63d0\u4f9b\u7684\u670d\u52d9\u548c\u7c21\u55ae\u3001\u4f7f\u7528\u65b9\u4fbf\u4e14\u6027\u80fd\u5353\u8d8a\u7684\u7522\u54c1\u800c\u81ea\u8c6a\u3002<\/p>\n<p><img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/02\/BIOASSAY_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1-300x300.jpg\" alt=\"BIOASSAY_\u5de5\u4f5c\u5340\u57df 1\" \/><\/p>\n<h3><span style=\"color: #800000; font-size: 12pt;\"><strong>BioAssay Systems\u6aa2\u6e2c\u8a66\u5291\u76d2\u53ca\u8a66\u5291<\/strong><\/span><\/h3>\n<ul>\n<li><a href=\"https:\/\/www.bioassaysys.com\/products?caturl=Agriculture-Environment\">Agriculture &amp; Environment<\/a><\/li>\n<li><a href=\"https:\/\/www.bioassaysys.com\/products?caturl=Blood-Urine-Chemistry\">Blood &amp; Urine Chemistry<\/a><\/li>\n<li><a href=\"https:\/\/www.bioassaysys.com\/products?caturl=Cations-Anions\">Cations &amp; Anions<\/a><\/li>\n<li><a href=\"https:\/\/www.bioassaysys.com\/products?caturl=Diabetes-Obesity\">Diabetes &amp; Obesity<\/a><\/li>\n<li><a href=\"https:\/\/www.bioassaysys.com\/products?caturl=Enzyme-Activity\">Enzyme Activity<\/a><\/li>\n<li><a href=\"https:\/\/www.bioassaysys.com\/products?caturl=Food-Beverage-Analysis\">Food &amp; Beverage Analysis<\/a><\/li>\n<li><a href=\"https:\/\/www.bioassaysys.com\/products?caturl=HTS-Reagents-Kits\">HTS Reagents &amp; Kits<\/a><\/li>\n<li><a href=\"https:\/\/www.bioassaysys.com\/products?caturl=Inhibitor-HTS-Kits\">Inhibitor HTS Kits<\/a><\/li>\n<li><a href=\"https:\/\/www.bioassaysys.com\/products?caturl=Metabolism\">Metabolism<\/a><\/li>\n<li><a href=\"https:\/\/www.bioassaysys.com\/products?caturl=Oncology\">Oncology<\/a><\/li>\n<li><a href=\"https:\/\/www.bioassaysys.com\/products?caturl=Other-Reagents\">Other Reagents<\/a><\/li>\n<li><a href=\"https:\/\/www.bioassaysys.com\/products?caturl=Oxidative-Stress\">Oxidative Stress<\/a><\/li>\n<li><a href=\"https:\/\/www.bioassaysys.com\/products?caturl=Quick-Test-Strips\">Quick Test Strips<\/a><\/li>\n<li><a href=\"https:\/\/www.bioassaysys.com\/products?caturl=Signal-Transduction\">Signal Transduction<\/a><\/li>\n<\/ul>\n<p><a href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/06\/1-\u6ab8\u6aac\u9178\u9e7d-Citrate\u6aa2\u6e2c\u8a66\u5291\u76d2-BioAssay-Systems-Citrate-Assay-Kit-\u8ca8\u865fECIT-100.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/11\/%E6%93%8D%E4%BD%9C%E6%96%B9%E6%B3%95-300x122.jpg\" alt=\"\u64cd\u4f5c\u65b9\u6cd5\" width=\"148\" height=\"67\" \/><\/a><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/01\/%E7%B6%B2%E7%AB%99%E8%A6%8F%E6%A0%BC%E5%B0%8F%E5%9C%96-%E7%9B%B8%E9%97%9C%E8%A9%A6%E5%8A%91_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1-300x120.jpg\" alt=\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716 \u76f8\u95dc\u8a66\u5291_\u5de5\u4f5c\u5340\u57df 1\" width=\"158\" height=\"69\" \/><\/p>\n<table width=\"56%\">\n<thead>\n<tr>\n<td width=\"217\"><strong>Name<\/strong><\/td>\n<td width=\"109\"><strong>SKU<\/strong><\/td>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td><a href=\"https:\/\/bioassaysys.com\/albumin-quick-test-strips\/\" data-product_id=\"9129\">QuantiQuik\u2122 Albumin Quick Test Strips<\/a><\/td>\n<td><a href=\"https:\/\/bioassaysys.com\/albumin-quick-test-strips\/\" data-product_id=\"9129\">QQALBM10<\/a><\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/bioassaysys.com\/d-lactic-acid-quick-test-strips\/\" data-product_id=\"9130\">QuantiQuik\u2122 D-Lactic Acid Quick Test Strips<\/a><\/td>\n<td><a href=\"https:\/\/bioassaysys.com\/d-lactic-acid-quick-test-strips\/\" data-product_id=\"9130\">QQDLAC10<\/a><\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/bioassaysys.com\/glucose-quick-test-strips\/\" data-product_id=\"9133\">QuantiQuik\u2122 Glucose Quick Test Strips<\/a><\/td>\n<td><a href=\"https:\/\/bioassaysys.com\/glucose-quick-test-strips\/\" data-product_id=\"9133\">QQGLUC10<\/a><\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/bioassaysys.com\/histamine-quick-test-strips\/\" data-product_id=\"9174\">QuantiQuik\u2122 Histamine Quick Test Strips<\/a><\/td>\n<td><a href=\"https:\/\/bioassaysys.com\/histamine-quick-test-strips\/\" data-product_id=\"9174\">QQHIST10<\/a><\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/bioassaysys.com\/l-glutamate-quick-test-strips\/\" data-product_id=\"9134\">QuantiQuik\u2122 L-Glutamate Quick Test Strips<\/a><\/td>\n<td><a href=\"https:\/\/bioassaysys.com\/l-glutamate-quick-test-strips\/\" data-product_id=\"9134\">QQGLUT10<\/a><\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/bioassaysys.com\/l-lactic-acid-quick-test-strips\/\" data-product_id=\"9175\">QuantiQuik\u2122 L-Lactic Acid Quick Test Strips<\/a><\/td>\n<td><a href=\"https:\/\/bioassaysys.com\/l-lactic-acid-quick-test-strips\/\" data-product_id=\"9175\">QQLLAC10<\/a><\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/bioassaysys.com\/malic-acid-quick-test-strips\/\" data-product_id=\"9176\">QuantiQuik\u2122 Malic Acid Quick Test Strips<\/a><\/td>\n<td><a href=\"https:\/\/bioassaysys.com\/malic-acid-quick-test-strips\/\" data-product_id=\"9176\">QQMALA10<\/a><\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/bioassaysys.com\/oxalate-quick-test-strips\/\" data-product_id=\"9177\">QuantiQuik\u2122 Oxalate Quick Test Strips<\/a><\/td>\n<td><a href=\"https:\/\/bioassaysys.com\/oxalate-quick-test-strips\/\" data-product_id=\"9177\">QQOXLT10<\/a><\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/bioassaysys.com\/pyruvate-quick-test-strips\/\" data-product_id=\"9178\">QuantiQuik\u2122 Pyruvate Quick Test Strips<\/a><\/td>\n<td><a href=\"https:\/\/bioassaysys.com\/pyruvate-quick-test-strips\/\" data-product_id=\"9178\">QQPYR10<\/a><\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/bioassaysys.com\/urea-bun-quick-test-strips\/\" data-product_id=\"9179\">QuantiQuik\u2122 Urea (BUN) Quick Test Strips<\/a><\/td>\n<td><a href=\"https:\/\/bioassaysys.com\/urea-bun-quick-test-strips\/\" data-product_id=\"9179\">QQUREA10<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u6ab8\u6aac\u9178\u9e7d( Citrate)\u6aa2\u6e2c\u8a66\u5291\u76d2(BioAssay Systems&#8217; Citrate Ass [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":37735,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5339],"tags":[5892],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/43434"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=43434"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/43434\/revisions"}],"predecessor-version":[{"id":43437,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/43434\/revisions\/43437"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/37735"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=43434"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=43434"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=43434"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}