{"id":42990,"date":"2023-06-09T17:47:15","date_gmt":"2023-06-09T09:47:15","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=42990"},"modified":"2023-06-13T17:51:25","modified_gmt":"2023-06-13T09:51:25","slug":"cd45%e9%87%8d%e7%b5%84%e8%9b%8b%e7%99%bd-t-b-cell-surface-proteins-bioworld%e8%b2%a8%e8%99%9fncp0211","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=42990","title":{"rendered":"CD45\u91cd\u7d44\u86cb\u767d | T\/B cell surface proteins BioWorld\u8ca8\u865fNCP0211"},"content":{"rendered":"

CD45\u91cd\u7d44\u86cb\u767d | T\/B cell surface proteins BioWorld\u8ca8\u865fNCP0211<\/span><\/strong><\/a><\/span><\/p>\n

CD45 Recombinant Protein<\/p>\n

\u86cb\u767d\u78f7\u9178\u9176 (The protein phosphatase , PTP) \u53d7\u9ad4 CD45 \u662f\u4e00\u7a2e I \u578b\u8de8\u819c\u86cb\u767d( type I transmembrane protein )\uff0c\u7531\u4e00\u5c0d\u7d30\u80de\u5167\u916a\u6c28\u9178\u78f7\u9178\u9176\u7d50\u69cb\u57df(intracellular tyrosine phosphatase domains )\u548c\u53ef\u8b8a\u526a\u63a5(alternative splicing)\u7522\u751f\u7684\u7d30\u80de\u5916\u7d50\u69cb\u57df\u7d44\u6210\u3002 CD45 \u7684\u50ac\u5316\u6d3b\u6027\u662f\u7b2c\u4e00\u500b\u78f7\u9178\u9176\u7d50\u69cb\u57df ( first\u00a0 phosphatase domain, D1) \u7684\u51fd\u6578\uff0c\u800c\u7b2c\u4e8c\u500b\u78f7\u9178\u9176\u7d50\u69cb\u57df ( second phosphatase domain, D2) \u53ef\u4ee5\u8207\u7b2c\u4e00\u500b\u7d50\u69cb\u57df\u76f8\u4e92\u4f5c\u7528\u4e26\u4f7f\u5176\u7a69\u5b9a\uff0c\u6216\u52df\u96c6\/\u7d50\u5408\u5e95\u7269\u3002 CD45 \u76f4\u63a5\u8207\u6297\u539f\u53d7\u9ad4\u8907\u5408\u86cb\u767d(antigen receptor complex proteins)\u76f8\u4e92\u4f5c\u7528\u6216\u6fc0\u6d3b\u53c3\u8207\u8abf\u7bc0 T \u7d30\u80de\u548c B \u7d30\u80de\u6297\u539f\u53d7\u9ad4\u4fe1\u865f\u50b3\u5c0e\u7684 Src \u5bb6\u65cf\u6fc0\u9176(Src family kinases)\u3002\u5177\u9ad4\u800c\u8a00\uff0cCD45 \u4f7f Src \u5bb6\u65cf\u6fc0\u9176 Lck ( Src-family kinases Lck )\u548c Fyn \u5728\u5176\u4fdd\u5b88\u7684\u8ca0\u8abf\u7bc0( conserved negative regulatory)\u7fa7\u57fa\u672b\u7aef\u916a\u6c28\u9178\u6b98\u57fa( carboxy-terminal tyrosine residues)\u8655\u53bb\u78f7\u9178\u5316\u4e26\u4e0a\u8abf\u6fc0\u9176\u6d3b\u6027\u3002\u76f8\u53cd\uff0c\u7814\u7a76\u8868\u660e CD45 \u9084\u53ef\u4ee5\u901a\u904e\u4f7f\u6b63\u8abf\u7bc0\u81ea\u78f7\u9178\u5316\u4f4d\u9ede\u53bb\u78f7\u9178\u5316\u4f86\u6291\u5236 Lck \u548c Fyn\u3002 CD45 \u4f3c\u4e4e\u65e2\u662f\u6b63\u8abf\u7bc0\u56e0\u5b50\u53c8\u662f\u8ca0\u8abf\u7bc0\u56e0\u5b50(negative regulator)\uff0c\u6839\u64da\u7279\u5b9a\u523a\u6fc0\u548c\u7d30\u80de\u985e\u578b\u50b3\u5c0e\u4fe1\u865f\u3002\u4eba\u985e\u767d\u7d30\u80de(Human leukocytes )\u5305\u62ec\u6dcb\u5df4\u7d30\u80de( lymphocytes)\u3001\u55dc\u9178\u6027\u7c92\u7d30\u80de(eosinophils)\u3001\u55ae\u6838\u7d30\u80de(monocytes)\u3001\u55dc\u9e7c\u6027\u7c92\u7d30\u80de(basophils)\u548c\u4e2d\u6027\u7c92\u7d30\u80de(neutrophils )\u8868\u9054 CD45\uff0c\u800c\u7d05\u7d30\u80de( erythrocytes )\u548c\u8840\u5c0f\u677f(platelets)\u4e0d\u8868\u9054 CD45\u3002<\/p>\n

https:\/\/bioworlde.com\/<\/a><\/p>\n

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\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Product Name<\/td>\nCD45 Recombinant Protein<\/td>\n<\/tr>\n
Catalog No.<\/td>\nNCP0211<\/td>\n<\/tr>\n
Swiss-Prot<\/td>\nP08575<\/a><\/td>\n<\/tr>\n
Host<\/td>\nE.coli<\/td>\n<\/tr>\n
Tag<\/td>\nHis-tag in C-terminal<\/td>\n<\/tr>\n
Restriction sites<\/td>\nNdeI-XhoI<\/td>\n<\/tr>\n
Background<\/td>\n<\/td>\n<\/tr>\n
Soluble<\/td>\nPBS, 4M Urea, PH7.4<\/td>\n<\/tr>\n
Purification&Purity<\/td>\nTransferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).<\/td>\n<\/tr>\n
Concentration<\/td>\n\u22650.5mg\/ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Product Name :<\/b>
\nCD45 Recombinant Protein<\/p>\n

Swiss-Prot :<\/b>
\nP08575<\/a><\/p>\n

Host :<\/b>
\nE.coli<\/p>\n

Tag :<\/b>
\n\u22650.5mg\/ml<\/p>\n

Restriction sites :<\/b>
\nNdeI-XhoI<\/p>\n

Soluble :<\/b>
\nPBS, 4M Urea, PH7.4<\/p>\n

Purification&Purity :<\/b>
\nTransferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).<\/p>\n

Storage&Stability :<\/b>
\nStore at 4\u00b0C short term. Aliquot and store at -20\u00b0C long term. Avoid freeze-thaw cycles.<\/p>\n

Expression vector :<\/b>
\npet-22b(+)<\/p>\n

BiowMW :<\/b>
\n~61kDa<\/p>\n","protected":false},"excerpt":{"rendered":"

CD45\u91cd\u7d44\u86cb\u767d | T\/B cell surface proteins BioWorld\u8ca8\u865fNCP0211 […]<\/p>\n","protected":false},"author":3,"featured_media":31670,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5799],"tags":[5830],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42990"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=42990"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42990\/revisions"}],"predecessor-version":[{"id":42991,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42990\/revisions\/42991"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/31670"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=42990"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=42990"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=42990"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}