{"id":42966,"date":"2023-06-09T15:55:07","date_gmt":"2023-06-09T07:55:07","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=42966"},"modified":"2023-06-09T17:14:25","modified_gmt":"2023-06-09T09:14:25","slug":"cd109-recombinant-protein-%e9%87%8d%e7%b5%84%e8%9b%8b%e7%99%bd-t-b-cell-surface-proteins-bioworld%e8%b2%a8%e8%99%9fncp0172","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=42966","title":{"rendered":"CD109 Recombinant Protein\u00a0 \u91cd\u7d44\u86cb\u767d | T\/B cell surface proteins BioWorld\u8ca8\u865fNCP0172"},"content":{"rendered":"

CD109\u00a0 \u91cd\u7d44\u86cb\u767d | T\/B cell surface proteins BioWorld\u8ca8\u865fNCP0172<\/span><\/strong><\/span><\/a><\/span><\/p>\n

CD109 \u662f\u7cd6\u57fa\u78f7\u8102\u91af\u808c\u9187 (glycosylphosphatidylinositol,GPI) \u9023\u63a5\u7684\u7cd6\u86cb\u767d(glycoprotein )\uff0c\u5c6c\u65bc\u542b\u786b\u916f\u86cb\u767d\u7684 alpha2-\u5de8\u7403\u86cb\u767d\u5bb6\u65cf(alpha2-macroglobulin family)\u3002 CD109 \u8207 TGF-\u03b2 \u53d7\u9ad4 I (TGF-beta receptor I, TbRI) \u76f8\u95dc\u4e26\u6291\u5236 TGF-\u03b2 \u4fe1\u865f\u50b3\u5c0e\u3002 CD109 \u5728\u5176\u5f17\u6797\u86cb\u767d\u9176\u5207\u5272\u4f4d\u9ede(Furin cleavage site )\u7684\u5207\u5272\u5c0e\u81f4\u91cb\u653e\u5176\u5927\u7684\u6c28\u57fa\u672b\u7aef\u7d50\u69cb\u57df(amino-terminal domain)\uff0c\u7136\u5f8c\u8a72\u7d50\u69cb\u57df\u8207 TGF-\u03b2 \u53d7\u9ad4 I (TGF-beta receptor I)\u7d50\u5408\u4ee5\u6291\u5236 TGF-\u03b2 \u4fe1\u865f\u50b3\u5c0e(TGF-beta signaling)\u3002 CD109 \u5728 CD34+ \u9aa8\u9ad3\u7d30\u80de(bone marrow cells)\u548c\u9593\u5145\u8cea\u5e79\u7d30\u80de(mesenchymal stem cells)\u3001\u6d3b\u5316\u7684\u8840\u5c0f\u677f( activated platelets)\u3001\u6d3b\u5316\u7684 T \u7d30\u80de(activated T cells)\u3001\u5167\u76ae\u7d30\u80de\u548c\u591a\u7a2e\u816b\u7624\u7684\u5b50\u96c6\u4e0a\u8868\u9054\u3002\u5347\u9ad8\u7684 CD109(Elevated CD109 expression) \u8868\u9054\u88ab\u8a8d\u70ba\u662f\u5e7e\u7a2e\u764c\u75c7\u7684\u8a3a\u65b7\/\u9810\u5f8c\u6a19\u8a8c\u7269\u3002<\/p>\n

https:\/\/bioworlde.com\/<\/a><\/p>\n

<\/p>\n

 <\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Product Name<\/td>\nCD109 Recombinant Protein<\/td>\n<\/tr>\n
Catalog No.<\/td>\nNCP0172<\/td>\n<\/tr>\n
Swiss-Prot<\/td>\nQ6YHK3<\/a><\/td>\n<\/tr>\n
Host<\/td>\nE.coli<\/td>\n<\/tr>\n
Tag<\/td>\nHis-tag in C-terminal<\/td>\n<\/tr>\n
Restriction sites<\/td>\nNdeI-XhoI<\/td>\n<\/tr>\n
Background<\/td>\n<\/td>\n<\/tr>\n
Soluble<\/td>\nPBS, 4M Urea, PH7.4<\/td>\n<\/tr>\n
Purification&Purity<\/td>\nTransferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).<\/td>\n<\/tr>\n
Concentration<\/td>\n\u22650.5mg\/ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

<\/p>\n

Restriction sites :<\/b><\/span>
\nNdeI-XhoI<\/p>\n

Background :<\/b><\/span>
\nCD109 is a glycosylphosphatidylinositol (GPI)-linked glycoprotein that belongs to the alpha2-macroglobulin family of thioester containing proteins. CD109 is associated with TGF-beta receptor I (TbRI) and inhibits TGF-beta signaling. Cleavage of CD109 at its Furin cleavage site results in the release of its large amino-terminal domain, which then binds to the TGF-beta receptor I to inhibit TGF-beta signaling. CD109 is expressed on a subset of CD34+ bone marrow cells and mesenchymal stem cells, activated platelets, activated T cells, endothelial cells, and a wide variety of tumors. Elevated CD109 expression has been considered a diagnostic\/prognostic marker for several types of cancers.<\/p>\n

Soluble :<\/b><\/span>
\nPBS, 4M Urea, PH7.4<\/p>\n

Purification&Purity :<\/span><\/strong><\/span>
\nTransferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).<\/p>\n

Storage&Stability :<\/span><\/strong><\/span>
\nStore at 4\u00b0C short term. Aliquot and store at -20\u00b0C long term. Avoid freeze-thaw cycles.<\/p>\n

Expression vector :<\/span><\/strong>
\npet-22b(+)<\/p>\n

BiowMW :<\/span><\/strong><\/span>
\n~36kDa<\/p>\n

Note :<\/b><\/span>
\nFor research use only, not for use in diagnostic procedure.<\/p>\n

concentration :<\/span><\/strong><\/span>
\n\u22650.5mg\/ml<\/p>\n","protected":false},"excerpt":{"rendered":"

CD109\u00a0 \u91cd\u7d44\u86cb\u767d | T\/B cell surface proteins BioWorld\u8ca8\u865fNCP01 […]<\/p>\n","protected":false},"author":3,"featured_media":31670,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5799],"tags":[5823],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42966"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=42966"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42966\/revisions"}],"predecessor-version":[{"id":42976,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42966\/revisions\/42976"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/31670"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=42966"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=42966"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=42966"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}