{"id":42925,"date":"2023-06-08T17:06:28","date_gmt":"2023-06-08T09:06:28","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=42925"},"modified":"2023-06-09T17:12:45","modified_gmt":"2023-06-09T09:12:45","slug":"%e8%b0%b7%e6%b0%a8%e9%86%af%e8%83%ba%e5%90%88%e6%88%90%e9%85%b6glutamine-synthetase-activity-gs%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-bioworld%e8%b2%a8%e8%99%9fask1021","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=42925","title":{"rendered":"\u8c37\u6c28\u91af\u80fa\u5408\u6210\u9176(Glutamine Synthetase Activity, GS)\u6aa2\u6e2c\u8a66\u5291\u76d2 | BioWorld\u8ca8\u865fASK1021\u00a0"},"content":{"rendered":"

\"\u6a19\u7c6405\"\u8c37\u6c28\u91af\u80fa\u5408\u6210\u9176(Glutamine Synthetase Activity, GS)\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a>\u00a0| Glutamine Synthetase Microplate Assay Kit \u00a0BioWorld\u8ca8\u865fASK1021<\/a>\u00a0<\/span><\/strong><\/p>\n

Glutamine Synthetase Microplate Assay Kit<\/p>\n

\u8c37\u6c28\u91af\u80fa\u5408\u6210\u9176\u6d3b\u6027(Glutamine Synthetase Activity)\u7684\u6aa2\u6e2c\u548c\u5b9a\u91cf\u5957\u7d44<\/p>\n

\u8c37\u6c28\u91af\u80fa\u5408\u6210\u9176(Glutamine Synthetase Activity, GS)\u4e3b\u8981\u5b58\u5728\u65bc\u690d\u7269\u4e2d\uff0c\u662f\u9ad4\u5167\u540c\u5316\u6c28(assimilation of ammonia)\u7684\u95dc\u9375\u9176\u4e4b\u4e00\u3002(Glutamine Synthetase Activity, GS)\u80fd\u50ac\u5316\u92a8\u96e2\u5b50\u548c\u8c37\u6c28\u9178\u5408\u6210\u8c37\u6c28\u91af\u80faGln( glutamine Gln)\uff0c\u4e0d\u50c5\u53ef\u4ee5\u9632\u6b62\u904e\u91cf\u7684\u751f\u7269\u6bd2\u6027\u92a8\u96e2\u5b50(biological toxic ammonium ions)\uff0c\u800c\u4e14\u8c37\u6c28\u91af\u80faGln( glutamine Gln)\u4e3b\u8981\u4ee5\u6c28\u7684\u5f62\u5f0f\u5132\u5b58\u548c\u904b\u8f38\u3002\u5728ATP\u548cMg2+\u5b58\u5728\u4e0b\uff0c(Glutamine Synthetase Activity, GS)\u80fd\u50ac\u5316\u92a8\u96e2\u5b50\u548c\u8c37\u6c28\u9178\u5408\u6210\u8c37\u6c28\u91af\u80faGln( glutamine Gln)\uff1b\u8c37\u6c28\u91af\u80faGln( glutamine Gln)\u9032\u4e00\u6b65\u8f49\u5316\u70ba\u03b3-\u8c37\u6c28\u91af\u7570\u7fa5\u809f\u9178(gamma-glutamyl hydroxamic acid)\uff0c\u5728\u9178\u6027\u689d\u4ef6\u4e0b\u5f62\u6210\u7d05\u8272\u9435\u7d61\u5408\u7269\uff1b\u8a72\u7d61\u5408\u7269\u5728 540nm \u8655\u6709\u6700\u5927\u5438\u6536\u5cf0\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Product Name<\/td>\nGlutamine Synthetase Microplate Assay Kit<\/td>\n<\/tr>\n
Catalog No.<\/td>\nASK1021<\/td>\n<\/tr>\n
Reactivity<\/td>\nAll<\/td>\n<\/tr>\n
Applications<\/td>\nFunctional Assay<\/td>\n<\/tr>\n
Description<\/td>\nDetection and Quantification of Glutamine Synthetase Activity.<\/td>\n<\/tr>\n
Alternative Names<\/td>\nEC 6.3.1.2; Glutamate-ammonia ligase; Glutamine synthetase; GS<\/td>\n<\/tr>\n
CAS Number<\/td>\n9023-70-5<\/td>\n<\/tr>\n
Detection Method<\/td>\nColorimetric<\/td>\n<\/tr>\n
Sample Types<\/td>\nTissue extracts, Cell lysate<\/td>\n<\/tr>\n
Storage\/Stability<\/td>\nShipped and store at 4\u00b0C for 6 months.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

<\/p>\n

1. For cell and bacteria samples<\/p>\n

Collect cell or bacteria into centrifuge tube, discard the supernatant after centrifugation, add 1 ml Assay buffer for 5 \u00d7 106<\/sup> cell or bacteria, sonicate (with power 20%, sonication 3s, intervation 10s, repeat 30 times); centrifuged at 8000g 4 \u00b0C for 10 minutes, take the supernatant into a new centrifuge tube and keep it on ice for detection.<\/p>\n

2. For tissue samples Weigh out 0.1 g tissue, homogenize with 1 ml Assay buffer on ice, centrifuged at 8000g 4 \u00b0C for 10 minutes, take the supernatant into a new centrifuge tube and keep it on ice for detection.<\/p>\n

3. For liquid samples Detect directly.<\/p>\n

\u5373\u7528\u578bIHC Detection System kit\u00a0<\/a> | \u725b\u8840\u6e05\u767d\u86cb\u767d\u7c89 (BSA)\u00a0<\/a> |<\/p>\n

<\/p>\n

Bioworld\u53f0\u7063\u4ee3\u7406\u5546-BRAND\/AGENCY\/\u4ee3\u7406\u54c1\u724c<\/span>\/ Bioworld\u53f0\u7063\u4ee3\u7406\u5546<\/span><\/strong><\/span><\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u8c37\u6c28\u91af\u80fa\u5408\u6210\u9176(Glutamine Synthetase Activity, GS)\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0| Gluta […]<\/p>\n","protected":false},"author":3,"featured_media":31670,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5799],"tags":[5814,5813],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42925"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=42925"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42925\/revisions"}],"predecessor-version":[{"id":42933,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42925\/revisions\/42933"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/31670"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=42925"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=42925"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=42925"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}