{"id":42911,"date":"2023-06-08T15:21:26","date_gmt":"2023-06-08T07:21:26","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=42911"},"modified":"2023-06-09T16:30:48","modified_gmt":"2023-06-09T08:30:48","slug":"%e7%8b%97%e8%82%8c%e9%88%a3%e8%9b%8b%e7%99%bd-i%ef%bc%8c%e5%bf%83%e8%82%8c-tnni3-elisa-%e8%a9%a6%e5%8a%91%e7%9b%92-dog-troponin-i-cardiac-muscle-tnni3-elisa-kit-abbexa%e8%b2%a8%e8%99%9fabx257866","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=42911","title":{"rendered":"\u72ac\u808c\u9223\u86cb\u767d I\uff0c\u5fc3\u808c (TNNI3) ELISA \u8a66\u5291\u76d2| Dog Troponin I, Cardiac Muscle (TNNI3) ELISA Kit Abbexa\u8ca8\u865fabx257866"},"content":{"rendered":"

\u72ac\u808c\u9223\u86cb\u767d I\uff0c\u5fc3\u808c (TNNI3) ELISA \u8a66\u5291\u76d2| Dog Troponin I, Cardiac Muscle (TNNI3) ELISA Kit Abbexa\u8ca8\u865fabx257866<\/a><\/span><\/h4>\n

Dog Troponin I, Cardiac Muscle (TNNI3) ELISA Kit<\/p>\n

Dog Troponin I, Cardiac Muscle (TNNI3) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Dog Troponin I, Cardiac Muscle (TNNI3) concentrations in serum, plasma and other biological fluids.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Target<\/td>\nTroponin I, Cardiac Muscle (TNNI3)<\/td>\n<\/tr>\n
Reactivity<\/td>\nDog<\/td>\n<\/tr>\n
Tested Applications<\/td>\nELISA<\/td>\n<\/tr>\n
Recommended dilutions<\/td>\nOptimal dilutions\/concentrations should be determined by the end user.<\/td>\n<\/tr>\n
Storage<\/td>\nShipped at 4 \u00b0C. Upon receipt, store the kit according to the storage instruction in the kit’s manual.<\/td>\n<\/tr>\n
Validity<\/td>\nThe validity for this kit is 6 months.<\/td>\n<\/tr>\n
Stability<\/td>\nThe stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.<\/td>\n<\/tr>\n
UniProt Primary AC<\/td>\nQ8MKD5 (UniProt<\/strong><\/a>,\u00a0ExPASy<\/strong><\/a>)<\/td>\n<\/tr>\n
UniProt Entry Name<\/td>\nTNNI3_CANLF<\/td>\n<\/tr>\n
Gene Symbol<\/td>\nTNNI3<\/td>\n<\/tr>\n
KEGG<\/td>\ncfa:403566<\/a><\/td>\n<\/tr>\n
Test Range<\/td>\n31.2 pg\/ml – 2000 pg\/ml<\/td>\n<\/tr>\n
Sensitivity<\/td>\n18.8 pg\/ml<\/td>\n<\/tr>\n
Standard Form<\/td>\nLyophilized<\/td>\n<\/tr>\n
Detection Method<\/td>\nColorimetric<\/td>\n<\/tr>\n
Assay Type<\/td>\nSandwich<\/td>\n<\/tr>\n
Assay Data<\/td>\nQuantitative<\/td>\n<\/tr>\n
Sample Type<\/td>\nSerum, plasma and other biological fluids.<\/td>\n<\/tr>\n
Kit Components<\/td>\nThe kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.<\/p>\n
    \n
  • Pre-coated 96-Well Microplate<\/li>\n
  • Standard<\/li>\n
  • Standard Diluent Buffer<\/li>\n
  • Wash Buffer<\/li>\n
  • Detection Reagent A<\/li>\n
  • Detection Reagent B<\/li>\n
  • Diluent A<\/li>\n
  • Diluent B<\/li>\n
  • TMB Substrate<\/li>\n
  • Stop Solution<\/li>\n
  • Plate Sealer<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
Material Required But Not Provided<\/td>\n\n
    \n
  • 37\u00b0C incubator<\/li>\n
  • Multi and single channel pipettes and sterile pipette tips<\/li>\n
  • Squirt bottle or automated microplate washer<\/li>\n
  • 1.5 ml tubes<\/li>\n
  • Distilled water<\/li>\n
  • Absorbent filter papers<\/li>\n
  • 100 ml and 1 liter graduated cylinders<\/li>\n
  • Microplate reader (wavelength: 450 nm)<\/li>\n
  • ELISA Shaker<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
Assay Procedure<\/td>\nThis procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.<\/p>\n
    \n
  • 1) Set standard, test samples and control wells.<\/li>\n<\/ul>\n
      \n
    • 2) Aliquot 100 \u00b5l of diluted standard into the standard wells.<\/li>\n<\/ul>\n
        \n
      • 3) Aliquot 100 \u00b5l of Standard Diluent buffer into control (zero) well.<\/li>\n<\/ul>\n
          \n
        • 4) Aliquot 100 \u00b5l of diluted samples into the sample wells. Incubate for 1 hr at 37 \u00b0C.<\/li>\n<\/ul>\n
            \n
          • 5) Aliquot 100 \u00b5l of Detection Reagent A to each well. Incubate for 1 hr at 37 \u00b0C.<\/li>\n<\/ul>\n
              \n
            • 6) Wash 3 times.<\/li>\n<\/ul>\n
                \n
              • 7) Aliquot 100 \u00b5l of Detection Reagent B to each well. Incubate for 90 mins at 37 \u00b0C.<\/li>\n<\/ul>\n
                  \n
                • 8) Wash 5 times.<\/li>\n<\/ul>\n
                    \n
                  • 9) Aliquot 90 \u00b5l of TMB Substrate to each well. Incubate for 10-20 mins at 37 \u00b0C.<\/li>\n<\/ul>\n
                      \n
                    • 10) Aliquot 50 \u00b5l of Stop Solution.<\/li>\n<\/ul>\n
                        \n
                      • 11) Measure the OD at 450 nm.<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
Assay Precision<\/td>\n\n

Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Troponin I, Cardiac Muscle (TNNI3) were tested 20 times on one plate, respectively.<\/p>\n

Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Troponin I, Cardiac Muscle (TNNI3) were tested on 3 different plates, 8 replicates in each plate.<\/p>\n

CV (%) = (Standard Deviation \/ mean) \u00d7 100<\/p>\n

Intra-Assay: CV<10%<\/p>\n

Inter-Assay: CV<10%<\/td>\n<\/tr>\n

Availability<\/td>\nShipped within 5-12 working days.<\/td>\n<\/tr>\n
Note<\/td>\n\n

This product is for research use only.<\/p>\n

The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments.<\/p>\n

Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u8840\u5c3f\u7d20\u6c2e\uff08Blood Urea Nitrogen , BUN\uff09\u6aa2\u6e2c<\/span><\/a>\u00a0|\u00a0\u808c\u9150\u6aa2\u6e2c\u8a66\u5291\u76d2(Creatinine Assay Kit)\u00a0<\/a>\u00a0|\u00a0UA\u5c3f\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0Calcium\u9223\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u9382\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

\u72ac\u808c\u9223\u86cb\u767d I\uff0c\u5fc3\u808c (TNNI3) ELISA \u8a66\u5291\u76d2| Dog Troponin I, Cardiac M […]<\/p>\n","protected":false},"author":3,"featured_media":33274,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5340],"tags":[5810,5809],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42911"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=42911"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42911\/revisions"}],"predecessor-version":[{"id":42940,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42911\/revisions\/42940"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/33274"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=42911"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=42911"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=42911"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}