{"id":42852,"date":"2023-06-02T16:47:34","date_gmt":"2023-06-02T08:47:34","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=42852"},"modified":"2023-06-05T18:44:06","modified_gmt":"2023-06-05T10:44:06","slug":"uch-l1-%e6%8a%91%e5%88%b6%e5%8a%91%e7%af%a9%e9%81%b8%e8%a9%a6%e5%8a%91%e7%9b%92-uch-l1-inhibitor-screening-kit-%e8%b2%a8%e8%99%9fab283392-k484-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=42852","title":{"rendered":"UCH-L1 \u6291\u5236\u5291\u7be9\u9078\u8a66\u5291\u76d2 | UCH-L1 Inhibitor Screening Kit \u8ca8\u865fab283392 (K484-100)"},"content":{"rendered":"

UCH-L1 \u6291\u5236\u5291\u7be9\u9078\u8a66\u5291\u76d2<\/strong><\/span>| Abcam \u8ca8\u865fab283392<\/strong><\/span><\/a><\/span><\/p>\n

PGP9.5 Deubiquitinase Inhibitor Screening Kit (ab283392)<\/span><\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"<\/strong><\/p>\n

\u6cdb\u7d20 C \u672b\u7aef\u6c34\u89e3\u9176 1 (Ubiquitin C-terminal Hydrolase 1 , UCH-L1) \u662f\u4e00\u7a2e\u53bb\u6cdb\u7d20\u5316\u9176 (deubiquitinating enzyme, DUB)\uff0c\u4e3b\u8981\u5728\u795e\u7d93\u5143\u7d44\u7e54( neuronal tissue)\u4e2d\u8868\u9054\uff0c\u5728\u67d0\u4e9b\u8166\u7d44\u7e54(brain tissues)\u4e2d\u4f54\u7e3d\u86cb\u767d\u8cea\u7684 1-2%\u3002 UCH-L1 \u662f\u4e00\u7a2e\u534a\u80f1\u6c28\u9178\u86cb\u767d\u9176(cysteine protease)\uff0c\u80fd\u5920\u5207\u5272\u6cdb\u7d20\u5206\u5b50(ubiquitin molecule)\u7684\u7fa7\u57fa\u7aef( carboxyl end )\u8207\u6cdb\u7d20\u4fee\u98fe\u86cb\u767d\u4e0a\u7684\u8cf4\u6c28\u9178\u6b98\u57fa\u4e4b\u9593\u7684\u7570\u80bd\u9375(isopeptide bond )\u3002\u7531\u65bc\u6cdb\u7d20\u5316(ubiquitination)\u7528\u65bc\u9019\u4e9b\u4fee\u98fe\u86cb\u767d\u7684\u5b9a\u4f4d\u548c\u964d\u89e3\uff0c\u56e0\u6b64\u53bb\u6cdb\u7d20\u5316\u9176\u7684\u6b63\u5e38\u529f\u80fd\u5c0d\u65bc\u7d30\u80de\u6d3b\u529b\u548c\u5b8c\u6574\u6027\u81f3\u95dc\u91cd\u8981\u3002 UCH-L1 \u7684\u5931\u8abf\u8207\u5e15\u91d1\u68ee\u75c5(Parkinson\u2019s )\u548c\u963f\u723e\u8328\u6d77\u9ed8\u75c5(Alzheimer\u2019s diseases)\u7b49\u795e\u7d93\u7cfb\u7d71\u75be\u75c5\u4ee5\u53ca\u764c\u75c7\u4fb5\u8972(cancer invasion)\u7684\u75c5\u7406\u751f\u7406\u5b78\u6709\u95dc\u3002\u901a\u904e\u9451\u5b9a\u5177\u6709\u9ad8\u9078\u64c7\u6027\u7684\u6291\u5236\u5291\u53ef\u4ee5\u4fc3\u9032\u5c0d\u9019\u4e9b\u904e\u7a0b\u7684\u9032\u4e00\u6b65\u7406\u89e3\u3002\u5df2\u77e5\u6709 400 \u591a\u7a2e DUB \u9176\uff0c\u8a72\u9776\u6a19\u63d0\u4f9b\u4e86\u6bd4\u86cb\u767d\u9176\u9ad4\u7cfb\u7d71\u9ad8\u5f97\u591a\u7684\u9078\u64c7\u6027\u3002 BioVision\/ABCAM \u7684 UCH-L1 \u6291\u5236\u5291\u7be9\u9078\u8a66\u5291\u76d2(BioVision\u2019s UCH-L1 Inhibitor Screening Kit) \u5229\u7528\u6d3b\u6027 UCH-L1 \u53bb\u6cdb\u7d20\u5316\u9176\uff0c\u5207\u5272\u5408\u6210\u86cb\u767d\u5e95\u7269\u4ee5\u91cb\u653e\u6e38\u96e2\u87a2\u5149\u5718\u7684\u80fd\u529b\uff0c\u53ef\u4ee5\u4f7f\u7528\u87a2\u5149\u9176\u6a19\u5100\u8f15\u9b06\u91cf\u5316\uff08Ex\/Em = 350\/440 nm\uff09\u3002\u5c0f\u5206\u5b50\u6291\u5236\u5291\u53ef\u4ee5\u6e1b\u5c11\u6216\u6d88\u9664\u9019\u7a2e\u6d3b\u6027\u3002\u56e0\u6b64\uff0c\u8a72\u6291\u5236\u5291\u7be9\u9078\u8a66\u5291\u76d2\u53ef\u4ee5\u5feb\u901f\u53ef\u9760\u5730\u78ba\u5b9a\u5404\u7a2e\u5316\u5408\u7269\u5c0d UCH-L1 \u53bb\u6cdb\u7d20\u5316\u9176\u7684\u6291\u5236\u4f5c\u7528\uff0c\u4e26\u61c9\u7528\u65bc\u7be9\u9078\u65b0\u578b\u6291\u5236\u5291\u3002<\/p>\n

<\/p>\n

\u529f\u80fd\u7814\u7a76 – PGP9.5 \u53bb\u6cdb\u7d20\u5316\u9176\u6291\u5236\u5291\u7be9\u9078\u8a66\u5291\u76d2 (ab283392)<\/strong><\/span><\/div>\n
\n

\u63d0\u4f9b\u7684 PGP9.5 \u6291\u5236\u5291\u5c0d PGP9.5 \u9176\u6d3b\u6027\u7684\u6291\u5236\u6a23\u54c1\u3002<\/p>\n

<\/p>\n

\u529f\u80fd\u7814\u7a76 – PGP9.5 \u53bb\u6cdb\u7d20\u5316\u9176\u6291\u5236\u5291\u7be9\u9078\u8a66\u5291\u76d2 (ab283392)<\/span><\/strong><\/div>\n
\n

\u63d0\u4f9b\u7684 PGP9.5 \u6291\u88fd\u5291\u5c0d PGP9.5 \u9176\u6d3b\u6027\u7684\u6291\u5236\u6a23\u54c1\u3002<\/p>\n<\/div>\n<\/div>\n\n\n\n\n\n\n\n\n\n\n\n\n
abID<\/th>\nab283392 (K484-100)<\/td>\n<\/tr>\n
Cat # +Size<\/th>\nK484-100<\/td>\n<\/tr>\n
Size<\/th>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/th>\nFluorescence (Ex\/Em = 350\/440 nm)<\/td>\n<\/tr>\n
Applications<\/th>\nScreening potential inhibitors of UCH-L1
\nCharacterize\/study UCH-L1 inhibitors<\/td>\n<\/tr>\n
Features & Benefits<\/th>\n\u2022 Rapid, simple & reliable test for screening potential inhibitors of UCH-L1
\n\u2022 Includes UCH-L1 Inhibitor [LDN-57444]<\/td>\n<\/tr>\n
Kit Components<\/th>\n\u2022 UCH-L1 Assay Buffer
\n\u2022 UCH-L1 Substrate (in DMSO)
\n\u2022 UCH-L1 Enzyme
\n\u2022 UCH-L1 Inhibitor (LDN-57444)
\n\u2022 96-well Half-Area White Plate<\/td>\n<\/tr>\n
Storage Conditions<\/th>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/th>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/th>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

\"CUBEE\u684c\u4e0a\u578b\u5c0f\u578b\u96e2\u5fc3\u6a5f\"<\/a>\"WPACO8000\"<\/a>\"MUPID-2<\/a>\"BIODROP-\u5be6\u9a57\u8a2d\u50993\"\"ELISA<\/a><\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

UCH-L1 \u6291\u5236\u5291\u7be9\u9078\u8a66\u5291\u76d2| Abcam \u8ca8\u865fab283392 PGP9.5 Deubiquitinase […]<\/p>\n","protected":false},"author":3,"featured_media":37625,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261],"tags":[5789],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42852"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=42852"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42852\/revisions"}],"predecessor-version":[{"id":42854,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42852\/revisions\/42854"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/37625"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=42852"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=42852"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=42852"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}