{"id":42607,"date":"2023-05-23T11:43:53","date_gmt":"2023-05-23T03:43:53","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=42607"},"modified":"2023-06-19T18:15:17","modified_gmt":"2023-06-19T10:15:17","slug":"rscript-reverse-transcriptase-50-reactions-bio-helix-%e8%b2%a8%e8%99%9frt001-0050","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=42607","title":{"rendered":"\u9006\u8f49\u9304\u9176 RScript Reverse Transcriptase (50 Reactions) | Bio-Helix \u8ca8\u865fRT001-0050"},"content":{"rendered":"

\u9006\u8f49\u9304\u9176 RScript Reverse Transcriptase \/ 50 Reactions| Bio-Helix \u8ca8\u865fRT001-0050<\/strong><\/a><\/span><\/h4>\n

RScript Reverse Transcriptase \/ 50 Reactions<\/p>\n

\u7522\u54c1\u63cf\u8ff0:<\/strong><\/span><\/p>\n

Overcome the most challenging RNA structures over a wide temperature range.<\/strong><\/p>\n

Bio-Helix RScript \u9006\u8f49\u9304\u9176(Bio-Helix RScript Reverse Transcriptase) – \u5c08\u70ba\u7814\u7a76\u548c\u8a3a\u65b7\u61c9\u7528\u800c\u5275\u65b0\u8a2d\u8a08\uff0c\u6eff\u8db3\u6240\u6709\u7684 cDNA \u5408\u6210\u9700\u6c42\uff0c\u4e26\u514b\u670d\u5bec\u6eab\u5ea6\u7bc4\u570d\u5167\u6700\u5177\u6311\u6230\u6027\u7684\u4e8c\u7d1a RNA \u7d50\u69cb( secondary RNA structures)\u3002\u65b0\u4e00\u4ee3\u5de5\u7a0b\u91cd\u7d44 M-MLV \u9006\u8f49\u9304\u9176( M-MLV reverse transcriptase)\u5177\u6709\u66f4\u9ad8\u7684\u71b1\u7a69\u5b9a\u6027(thermostability)\u3001\u6301\u7e8c\u5408\u6210\u80fd\u529b(processivity)\u3001\u7a69\u5065\u6027(robustness)\u3001\u6700\u4f73 cDNA \u7522\u91cf\u3001\u7528\u65bc\u964d\u4f4e RNase H \u6d3b\u6027\u7684\u5c08\u6709\u4f4d\u9ede\u7a81\u8b8a\u4ee5\u53ca\u5ef6\u9577\u7684\u534a\u8870\u671f\uff0c\u662f\u4e16\u754c\u4e0a\u6700\u901a\u7528\u7684\u9006\u8f49\u9304\u9176( versatile reverse transcriptase )\u4e0d\u50c5\u7c21\u55ae\u5730\u6eff\u8db3\u5e38\u898f cDNA \u5408\u6210\u8981\u6c42\uff0c\u800c\u4e14\u5373\u4f7f\u662f\u624b\u982d\u6700\u5177\u6311\u6230\u6027\u7684 RNA \u6a23\u54c1\u4e5f\u80fd\u5be6\u73fe\u5353\u8d8a\u7684\u6027\u80fd\u3002<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

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Figure 1. Stability of RScript reverse transcriptase:<\/strong>
\nThe stability RT-qPCR data shows the performance of RScript reverse transcriptase is maintained without any significant alteration in performance at 37 \u00b0C after a period of 3 weeks (21 days), demonstrating its high resistance to temperature and time. The percentage of RScript reverse transcriptase activity was calculated by dividing values at each reaction temperature.<\/p>\n

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\nA: Bio-Helix\u00a0RScript reverse transcriptase #RT001-0050\/ RT001-0250<\/strong>
\nB: Promega Improm II #A3802
\nC: Thermo SuperScript III #18080093
\nD: SuperScript IV #18090010<\/p>\n

Figure 2. Enhancement of the thermostability:<\/strong>
\nReverse transcription of a 1 kb fragment ARHGAP29 RNA using\u00a0RScript<\/strong>\u00a0reverse transcriptase or competitor reverse transcriptases were used to carry this experiment. Regardless of the variations in temperatures (42 -60 \u00b0C) RScript reverse transcriptase shows a stable and superior performance at higher temperatures of up to 60 \u2103 compared to other brands. The molecular weight marker used was DNA Ladder 1kb #SDL-1000R.<\/p>\n

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Figure 3. Elongation of RNA templates up to 11 kb in length :<\/strong>
\nRScript\u00a0reverse transcriptase was used in a reaction with a range of human bladder cancer cell lines – 5637 (HTB-9) RNA. The resulting synthesized cDNA (8 kb ,11kb) was followed by PCR and visualized on a 2% agarose gel. The molecular weight marker used was DNA Ladder 1kb #SDL-1000R.<\/p>\n

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RScript Reverse Transcriptase 50 Reactions Bio-Helix \u8ca8\u865fRT001-0050<\/p>\n

Figure 4. Performance of One-Step RT-qPCR with RScript reverse transcriptase :<\/strong><\/p>\n

To evaluate the RT performance for the COVID-19 fragments, the N1 gene with 100 copies per reaction was tested in the one-step RT-qPCR. Experiments proved to be feasible and effective (mean Ct : 34.88).<\/p>\n

* COVID-19 fragments were originally obtained from Twist Synthetic SARS-CoV-2 RNA Controls (# EPI_ISL_1662307).<\/p>\n

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Red: Bio-HeliX RScript reverse transcriptase #RT001-0050
\nGreen: Improm-II #A3802
\nBlue: Superscript IV #18090010
\nFigure 5. Performing RT-qPCR with RScript reverse transcriptase :<\/strong>
\nAt a temperature of 55 \u00b0C, RScript reverse transcriptase exceeds performance demonstrating better efficiency and higher cDNA yields. RScript reverse transcriptase shows a stable and superior performance at 55 \u2103 compared to other brands. Therefore RScript reverse transcriptase does not encounter significant higher Ct values compared to Improm II and SuperScript IV\u00a0due to their low amounts of input cDNA.<\/p>\n

*Promega Improm-II #A3802 is a registered trademark of Promega, Thermo Superscript-III #18080093 and Superscript-IV #18090010 are registered trademarks of Thermo Scientific. The trademark holders are not affiliated with Bio-Helix Co., Ltd. and do not endorse these products.<\/p>\n

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\u7522\u54c1\u7279\u5fb5:<\/strong><\/span><\/p>\n