{"id":42591,"date":"2023-05-23T11:09:29","date_gmt":"2023-05-23T03:09:29","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=42591"},"modified":"2023-07-27T16:22:48","modified_gmt":"2023-07-27T08:22:48","slug":"panprobes-universal-qpcr-mastermix-bio-helix-%e8%b2%a8%e8%99%9fqpd01-0100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=42591","title":{"rendered":"PanProbes\u2122 Universal qPCR \u9810\u6df7\u6db2 MasterMix | Bio-Helix \u8ca8\u865fQPD01-0100"},"content":{"rendered":"

PanProbes\u2122 Universal qPCR\u9810\u6df7\u6db2MasterMix| Bio-Helix \u8ca8\u865fQPD01-0100<\/strong><\/a><\/span><\/h4>\n

PanProbes\u2122 Universal qPCR MasterMix<\/p>\n

\u7522\u54c1\u63cf\u8ff0:<\/strong><\/span><\/p>\n

PanProbesTM<\/sup> \u901a\u7528 qPCR \u9810\u6df7\u6db2(Universal qPCR Master Mix)\u662f\u4e00\u7a2e 2 \u500d\u6fc3\u7e2e( 2x concentrated)\u7684\u5373\u7528\u578b\u9810\u6df7\u6db2(ready-to-use master mix)\uff0c\u91dd\u5c0d\u57fa\u65bcprobe- PCR (probe-based real-time PCR )\u9032\u884c\u4e86\u512a\u5316\uff0c\u4e26\u8207real-time PCR systems\uff08\u4e0d\u4f9d\u8cf4 ROX \u548c\u4f9d\u8cf4 ROX\uff09\u517c\u5bb9\u3002\u5b83\u5305\u542b\u6297\u9ad4\u4ecb\u5c0e\u7684\u71b1\u555f\u52d5 Taq DNA\u805a\u5408\u9176( antibody-mediated hot-start Taq DNA polymerase)\u3001dNTP\u3001MgCl2\u3001\u589e\u5f37\u5291(enhancers)\u3001\u7a69\u5b9a\u5291(stabilizers )\u548c\u6210\u529f\u9032\u884c PCR \u53cd\u61c9\u7684\u5fc5\u9700\u54c1\u3002<\/p>\n

* \u589e\u5f37\u7684\u6297\u9ad4\u4ecb\u5c0e\u7684\u71b1\u555f\u52d5 DNA \u805a\u5408\u9176\u3002
\n* \u91dd\u5c0d\u57fa\u65bc\u63a2\u91dd\u7684\u5be6\u6642 PCR \u9032\u884c\u4e86\u512a\u5316\u3002
\n* \u8207\u5927\u591a\u6578 QPCR \u7cfb\u7d71\u517c\u5bb9\u3002<\/p>\n

Reaction Setup -Universal qPCR Master Mix\u00a0<\/span><\/strong><\/span><\/p>\n

1. Thaw PanProbes\u2122 Universal qPCR Master Mix and the rest of frozen reaction components to a temperature of 4\u00b0C. In order to entirely collect solutions, combine thoroughly and centrifuge briefly, then store at 4\u00b0C and avoid from light.<\/p>\n

2. Prepare (on ice or at room temperature) enough assay Master Mix for all reactions by adding all necessary components, except the DNA template, according to the recommendations in Table 1 (below).<\/p>\n

<\/p>\n

3. Combine the assay Master Mix thoroughly to ensure consistency and equally dispense the solution into each qPCR tube or into the wells of a qPCR plate. Employ good pipetting practice to ensure assay precision and accuracy.<\/p>\n

4. Add DNA samples (and DNase-free H2O if needed) to the PCR tubes or wells containing assay Master Mix (Table 1), seal the tubes or wells with flat caps or optically transparent film. Note: to ensure thorough mixing of reaction components, vortex for approximately 30 seconds (or more).<\/p>\n

5. Spin the tubes or plate to remove any air bubbles and collect the reaction mixture in the vessel bottom.<\/p>\n

6. Setup the thermal cycling protocol on a real-time PCR instrument according to Table 2. Note: optimization may be needed for better performance.<\/p>\n

7. Load the PCR tubes or plate into the real-time PCR instrument and commence the run.<\/p>\n

8. Perform data analysis according to the instrument-specific instructions.<\/p>\n

\u26ab Process in the thermal cycler for 35~45 cycles as follows:<\/p>\n

<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

<\/p>\n

Figure 1. Performance of PanProbes\u2122 Universal qPCR MasterMix<\/strong><\/p>\n

The SARS-CoV-2 nucleocapsid (N1) gene with serial diluted DNA template concentrations are prepared and conducted by qPCR. Based on the detected N1 probe fluorescence values, the result shows the Ct values corresponding to the different concentration.<\/p>\n

<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n
RK001-0050
\ncDNA\u5408\u6210KIT<\/td>\n		RScript\u2122 cDNA Synthesis Kit
\n(\u65b0\u4e00\u4ee3\u5de5\u7a0b\u91cd\u7d44 M-MLV \u9006\u8f49\u9304\u9176(M-MLV reverse transcriptase))
\nRScript cDNA \u5408\u6210\u8a66\u5291\u76d2(200 U\/\u03bcl)\u00a0<\/span><\/a><\/strong><\/td>\n		50 rxns<\/td>\n<\/tr>\n
RT001-0050
\nRT\u9006\u8f49\u9304\u9176<\/td>\n		RScript\u2122 Reverse Transcriptase 10,000U
\n(\u65b0\u4e00\u4ee3\u5de5\u7a0b\u91cd\u7d44 M-MLV \u9006\u8f49\u9304\u9176(M-MLV reverse transcriptase))
\n\u9006\u8f49\u9304\u9176 RScript Reverse Transcriptase<\/span><\/strong><\/a><\/td>\n		50 rxns
\n10,000U\/50\u03bcl<\/td>\n<\/tr>\n
RT001-0250
\nRT\u9006\u8f49\u9304\u9176<\/td>\n		RScript\u2122 Reverse Transcriptase 50,000U
\n(\u65b0\u4e00\u4ee3\u5de5\u7a0b\u91cd\u7d44 M-MLV \u9006\u8f49\u9304\u9176(M-MLV reverse transcriptase))
\n\u9006\u8f49\u9304\u9176RScript Reverse Transcriptase<\/span><\/strong><\/a><\/td>\n		250 rxns
\n50,000U\/250\u03bcl<\/td>\n<\/tr>\n
RI001-0125
\nRNase Inhibitor<\/td>\n		RIBOAssure\u2122 RNase Inhibitor 5,000U
\n(\u6291\u5236\u771f\u6838\u9176\u5982 RNase A\u3001RNase B \u548c RNase C \u6240\u8868\u73fe\u51fa\u7684\u6838\u7cd6\u6838\u9178\u9176 (RNase) \u6d3b\u6027)
\nRNase \u00a0\u6291\u5236\u5291<\/span><\/a><\/td>\n		125 rxns
\n5,000U\/125\u03bcl<\/td>\n<\/tr>\n
QPR01-0100
\n(Probe)<\/td>\n		PanProbes\u2122 One-Step RT-qPCR Kit (Probe)
\n(\u65b0\u4e00\u4ee3\u5de5\u7a0b\u91cd\u7d44 M-MLV \u9006\u8f49\u9304\u9176(M-MLV reverse transcriptase))
\n\u5f37\u5927\u7684 RNase \u6291\u5236\u5291\u6df7\u5408\u7269(RNase inhibitors mix)
\n\u589e\u5f37\u7684\u6297\u9ad4\u4ecb\u5c0e\u7684\u71b1\u555f\u52d5 DNA \u805a\u5408\u9176
\n(\u91dd\u5c0d\u57fa\u65bc\u63a2\u91dd\u7684real-time PCR \u9032\u884c\u4e86\u512a\u5316)
\n\u4e00\u6b65\u9a5f RT-qPCR <\/span><\/strong>\u8a66\u5291\u76d2<\/strong><\/span><\/a><\/td>\n		100 reactions
\n(20 \u03bcl vol)<\/td>\n<\/tr>\n
QPD01-0100
\n(Probe)<\/td>\n		PanProbes\u2122 Universal qPCR MasterMix
\n(\u91dd\u5c0d\u57fa\u65bcprobe- PCR (probe-based real-time PCR )\u9032\u884c\u4e86\u512a\u5316)
\n\u589e\u5f37\u7684\u6297\u9ad4\u4ecb\u5c0e\u7684\u71b1\u555f\u52d5 DNA \u805a\u5408\u9176
\nPanProbes<\/span><\/strong>\u2122 Universal qPCR<\/strong>\u9810\u6df7\u6db2<\/strong><\/span><\/a><\/td>\n		100 reactions
\n(20 \u03bcl vol)<\/td>\n<\/tr>\n
QSR01-0100
\n(SYBR\u00ae Green)<\/td>\n		PanGreen\u2122 One-Step RT-qPCR Kit (SYBR\u00ae Green)
\n(\u65b0\u4e00\u4ee3\u5de5\u7a0b\u91cd\u7d44 M-MLV \u9006\u8f49\u9304\u9176(M-MLV reverse transcriptase))
\n\u5f37\u5927\u7684 RNase \u6291\u5236\u5291\u6df7\u5408\u7269(RNase inhibitors mix)
\n\u589e\u5f37\u7684\u6297\u9ad4\u4ecb\u5c0e\u7684\u71b1\u555f\u52d5 DNA \u805a\u5408\u9176
\nUniversal SYBR\u00ae Green Master Mix\u00a0 2 \u500d\u6fc3\u7e2e\u7684\u5373\u7528\u578b\u6df7\u5408\u6db2 Master Mix
\n\u4e00\u500b\u6b65\u9a5fRT-qPCR <\/span><\/strong>\u8a66\u5291\u76d2PanGreen<\/strong><\/span><\/a><\/td>\n		100 reactions
\n(20 \u03bcl vol)<\/td>\n<\/tr>\n
QSD01-0100
\nSYBR\u00ae Green)<\/td>\n		PanGreen\u2122 Universal SYBR\u00ae Green Mastermix (SYBR\u00ae Green)
\n(2 \u500d\u6fc3\u7e2e\u7684\u5373\u7528\u578b Master Mix \u6df7\u5408\u6db2)
\n\u589e\u5f37\u7684\u6297\u9ad4\u4ecb\u5c0e\u7684\u71b1\u555f\u52d5 DNA \u805a\u5408\u9176
\n\u5373\u7528\u578b qPCR <\/span><\/strong>\u6df7\u5408\u6db2PanGreen<\/strong><\/span><\/a><\/td>\n		100 reactions
\n(20 \u03bcl vol)<\/td>\n<\/tr>\n
PDR03-0100<\/td>\nTotal RNA Isolation Reagent
\n(Reagent Based) for the tissue, cultured animal and bacterial cells, blood, and serum.
\nTotal RNA <\/span><\/strong>\u5206\u96e2\u8a66\u5291 (Reagent Based<\/strong>\uff09<\/strong><\/span><\/a><\/td>\n		100 rxns<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\"\"<\/p>\n

PCR\u6df7\u5408\u6db2<\/a>\u00a0|\u00a0<\/span>100bp-10kb DNA Ladder\u00a0<\/a>\u00a0|<\/span>\u00a0\u74ca\u8102\u81a0\u9ad4\u7c89Agarose<\/a>\u00a0|<\/span>\u00a0\u00a0DNA\u6838\u9178\u67d3\u5291<\/a>\u00a0|<\/span>\u00a0\u85cd\u5149\u89c0\u5bdf\u7bb1(\u542b\u767d\u5149)<\/a>\u00a0|<\/span>\u00a0Mupid-2\u00a0\u6c34\u5e73\u96fb\u6cf3\u69fd<\/a>\u00a0|<\/span><\/span><\/strong><\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716<\/p>\n

\"BIO-HIDEX\"<\/p>\n

bio-helix.com<\/p>\n

Bio-Helix\u6210\u7acb\u65bc2007\u5e74\uff0c\u5c08\u6ce8\u65bc\u70ba\u5168\u7403\u751f\u547d\u79d1\u5b78\u7814\u7a76\u4eba\u54e1\u63d0\u4f9b\u6700\u597d\u7684\u751f\u7269\u8a66\u5291\u3002\u5305\u62ec DNA \u6a19\u8a18\u7269\u3001\u86cb\u767d\u8cea\u6a19\u8a18\u7269\u3001\u9810\u88fd\u86cb\u767d\u8cea\u51dd\u81a0\u3001\u6838\u9178\u7d14\u5316\u3001PCR\u8a66\u5291\u7b49\u3002\u8207\u4e16\u754c\u5404\u5730\u5be6\u9a57\u5ba4\u7684\u79d1\u5b78\u5bb6\u5011\u4e00\u8d77\u52aa\u529b\uff0c\u4e0d\u65b7\u63d0\u9ad8\u8cea\u91cf\uff0c\u958b\u59cb\u8d0f\u5f97\u5ba2\u6236\u7684\u4fe1\u4efb\u548c\u8a55\u50f9\u3002\u54c1\u724c\u904d\u5e03\u56db\u5341\u591a\u500b\u570b\u5bb6\uff0c\u5df2\u6210\u70ba\u751f\u547d\u79d1\u5b78\u5be6\u9a57\u5ba4\u503c\u5f97\u4fe1\u8cf4\u548c\u6b61\u8fce\u7684\u8a66\u5291\u54c1\u724c\u3002Bio-Helix\u500b\u9858\u666f\uff0c\u5c31\u662f\u6709\u4e00\u5929\u6211\u5011\u7684\u58fd\u547d\u53ef\u4ee5\u5ef6\u9577\uff1b\u6240\u6709\u75be\u75c5\u7684\u65e9\u671f\u767c\u73fe\u548c\u96a8\u5f8c\u7684\u9069\u7576\u6cbb\u7642\u90fd\u53ef\u4ee5\u63d0\u9ad8\u751f\u6d3b\u8cea\u91cf\u3002\u4e2d\u570b\u53e4\u4eba\u8a8d\u70ba\uff0c\u826f\u91ab\u6cbb\u672a\u75c5\uff0c\u6cbb\u672a\u75c5\u3002\u5728 Bio-Helix \u4e2d\uff0c\u5275\u5efa\u4e86\u4e00\u500b\u65b0\u54c1\u724c LifeDirex \u4f86\u5be6\u73fe\u9019\u4e00\u9858\u666f\u3002 LifeDirex \u5c07\u5c08\u6ce8\u65bc\u70ba\u8a3a\u65b7\u5de5\u5177\u63d0\u4f9b\u6700\u597d\u548c\u6700\u5177\u5275\u65b0\u6027\u7684\u8a66\u5291\uff0c\u4ee5\u4fc3\u9032\u76e1\u53ef\u80fd\u65e9\u5730\u767c\u73fe\u75be\u75c5\u3002Bio-Helix\u4f7f\u547d\u662f\u63d0\u4f9b\u6700\u512a\u8cea\u7684\u7522\u54c1\u4e26\u958b\u767c\u65b0\u6280\u8853\uff0c\u5e6b\u52a9\u7814\u7a76\u4eba\u54e1\u627e\u5230\u5c0b\u6c42\u79d1\u5b78\u6210\u5c31\u7684\u89e3\u6c7a\u65b9\u6848\u3002\u6b64\u5916\uff0cBio-Helix\u8a8d\u70ba\u73fe\u5728\u662f\u9032\u5165\u9ad4\u5916\u8a3a\u65b7\u8a2d\u5099\uff08IVDD\uff09\u5e02\u5834\u7684\u6700\u4f73\u6642\u6a5f\u3002\u6211\u5011\u7684\u76ee\u6a19\u662f\u6210\u70ba\u7814\u7a76\u5be6\u9a57\u5ba4\u548cIVDD\u7684\u6700\u4f73\u89e3\u6c7a\u65b9\u6848\u63d0\u4f9b\u5546\u3002<\/p>\n

\u5728\u6539\u5584\u751f\u6d3b\u8cea\u91cf\u548c\u5be6\u73fe\u79d1\u5b78\u5353\u8d8a\u7684\u9858\u671b\u7684\u9a45\u4f7f\u4e0b\uff0cBio-Helix\u4ee5\u6700\u9ad8\u7684\u8aa0\u4fe1\u6a19\u6e96\u904b\u71df\u3002\u9084\u5c07\u9019\u7a2e\u7cbe\u795e\u5ef6\u4f38\u5230\u6211\u5011\u7684\u670d\u52d9\u548c\u5168\u7403\u50f9\u503c\u4e2d\uff0c\u4ee5\u63d0\u4f9b\u7d66\u4e16\u754c\u5404\u5730\u7684\u5ba2\u6236\u3002\u591a\u6a23\u5316\u7684\u54e1\u5de5\u968a\u4f0d\u548c\u5354\u4f5c\u7db2\u7d61\u5f62\u6210\u4e86\u516c\u53f8\u6587\u5316\u7684\u5b8c\u7f8e\u7d50\u5408\u3002 Bio-Helix \u4e0d\u50c5\u662f\u4e00\u5bb6\u6a5f\u6703\u5747\u7b49\u7684\u516c\u53f8\uff0c\u800c\u4e14\u9084\u52aa\u529b\u63d0\u4f9b\u53cb\u597d\u548c\u6210\u9577\u7684\u74b0\u5883\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
CAT#<\/td>\nNAME<\/td>\nSize<\/td>\n<\/tr>\n
BP001CU<\/td>\nBluPAD, LED Transilluminator, with mini Darkroom
\n(CE\/ETL, Patent No: M543442)<\/td>\n		Set<\/td>\n<\/tr>\n
PMI09-0500<\/td>\nIRIS9 Plus Prestained Protein Ladder
\n(9 pre-stained bands, 15-170 kDa)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
PMI11-0500<\/td>\nIRIS11 Prestained Protein Ladder
\n(11 pre-stained bands, 3-260 kDa)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
PMB01-0500<\/td>\nBlu10 Plus Prestained Protein Ladder
\n(10 pre-stained bands, 6.5-270 kDa)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
PMB10-0500<\/td>\nBlu10 Prestained Protein Ladder
\n(10 pre-stained bands, 11-180 kDa)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
PMB11-0500<\/td>\nBlu11 Prestained Protein Ladder
\n(11 pre-stained bands, 10-180 kDa)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
PMB12-0500<\/td>\nBlu12 Prestained Protein Ladder
\n(12 pre-stained bands, 11-245 kDa)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
PMB13-0500<\/td>\nBlu13 Prestained Protein Ladder
\n(13 pre-stained bands, 5-245 kDa)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
PMU12-0500<\/td>\nUNveil Unstained Protein Ladder, Broad Range
\n(12 unstained bands, 10-200 kDa)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
CCH321-B100ML<\/td>\nUltraScence Pico Plus Western Substrate
\n(Patent No: US10,711,185)<\/td>\n		100 ml
\n(50ml+50ml)<\/td>\n<\/tr>\n
CCH345-B100ML<\/td>\nUltraScence Pico Ultra Western Substrate
\n(Patent No: US10,711,185)<\/td>\n		100 ml
\n(50ml+50ml)<\/td>\n<\/tr>\n
CCH365-B100ML<\/td>\nUltraScence Femto Western Substrate
\n(Patent No: US10,711,185)<\/td>\n		100 ml
\n(50ml+50ml)<\/td>\n<\/tr>\n
CCH375-B100ML<\/td>\nUltraScence Femto Plus Western Substrate
\n(Patent No: US10,711,185)<\/td>\n		100 ml
\n(50ml+50ml)<\/td>\n<\/tr>\n
PS002-B500ML<\/td>\nCOOMASSIEnano Protein Staining Solution, 500ml<\/td>\n500 ml<\/td>\n<\/tr>\n
PS003-B500ML<\/td>\nPonceau S Protein Staining Solution<\/td>\n500 ml<\/td>\n<\/tr>\n
BS001-B500ML<\/td>\nOneStep Blocker
\nWestern Blocking Solution and Signal Enhancer<\/td>\n		500 ml<\/td>\n<\/tr>\n
TM001-B200ML<\/td>\nTMB ELISA Substrate<\/td>\n100 ml
\n(50ml+50ml)<\/td>\n<\/tr>\n
DM001-R500<\/td>\nBH 100bp DNA Ladder RTU
\n(11 bands, 100-1,500bps)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
DM003-R500<\/td>\nBH 100bp DNA Ladder H3 RTU
\n(12 bands, 100-3,000bps)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
DM012-R500<\/td>\nBH 50bp DNA Ladder RTU
\n(17 bands, 50-1,500bps)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
DM015-R500<\/td>\nBH 1Kb Plus DNA Ladder RTU
\n(13 bands, 100-10,000bps)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
DMF12-0100<\/td>\nOmniMARK 100 DNA Ladder RTU
\n(12 bands, 100-3,000bps, fluorescent dye)<\/td>\n		600 \u03bcl<\/td>\n<\/tr>\n
DMF13-0100<\/td>\nOmniMARK 1K DNA Ladder RTU
\n(13 bands, 100-10,000bps, fluorescent dye)<\/td>\n		600 \u03bcl<\/td>\n<\/tr>\n
LD011-1000<\/td>\nPrime Juice Preloading Fluorescent Stain<\/td>\n1 ml<\/td>\n<\/tr>\n
LD003-0500<\/td>\nNovel Green Plus (20,000X) DNA Staining Reagent<\/td>\n500 \u03bcl<\/td>\n<\/tr>\n
AGT002-0500<\/td>\nAgarose Tablets, 110 pcs<\/td>\n0.5g x 110<\/td>\n<\/tr>\n
MB755-0100<\/td>\nAgarose Powder 100g -Molecular Biology Grade<\/td>\n100 g<\/td>\n<\/tr>\n
MB755-0500<\/td>\nAgarose Powder 500g -Molecular Biology Grade<\/td>\n500 g<\/td>\n<\/tr>\n
MB101-0500<\/td>\nTaq DNA Polymerase<\/td>\n500U\/100\u03bcl<\/td>\n<\/tr>\n
DP001-0100<\/td>\nnanoTaq\u2122 Hot-Start DNA Polymerase<\/td>\n100 rxns
\n500U\/100\u03bcl<\/td>\n<\/tr>\n
DN001-0250<\/td>\n100 mM dNTP Set<\/td>\n4 x 250\u03bcl<\/td>\n<\/tr>\n
DN0010<\/td>\n10 mM dNTP Mix<\/td>\n1 ml<\/td>\n<\/tr>\n
MB200-P100<\/td>\n2X PCR SuperMix<\/td>\n100 rxns
\n2 x 1.25mL<\/td>\n<\/tr>\n
MBA01-0100<\/td>\nOmniPCR Supermix w Fluorescent dye<\/td>\n100 rxns
\n(2.5ml)<\/td>\n<\/tr>\n
RK001-0050<\/td>\nRScript\u2122 cDNA Synthesis Kit<\/td>\n50 rxns<\/td>\n<\/tr>\n
RT001-0050<\/td>\nRScript\u2122 Reverse Transcriptase 10,000U<\/td>\n50 rxns
\n10,000U\/50\u03bcl<\/td>\n<\/tr>\n
RT001-0250<\/td>\nRScript\u2122 Reverse Transcriptase 50,000U<\/td>\n250 rxns
\n50,000U\/250\u03bcl<\/td>\n<\/tr>\n
RI001-0125<\/td>\nRIBOAssure\u2122 RNase Inhibitor 5,000U<\/td>\n125 rxns
\n5,000U\/125\u03bcl<\/td>\n<\/tr>\n
QPR01-0100<\/td>\nPanProbes\u2122 One-Step RT-qPCR Kit<\/td>\n100 reactions
\n(20 \u03bcl vol)<\/td>\n<\/tr>\n
QPD01-0100<\/td>\nPanProbes\u2122 Universal qPCR MasterMix<\/td>\n100 reactions
\n(20 \u03bcl vol)<\/td>\n<\/tr>\n
QSR01-0100<\/td>\nPanGreen\u2122 One-Step RT-qPCR Kit<\/td>\n100 reactions
\n(20 \u03bcl vol)<\/td>\n<\/tr>\n
QSD01-0100<\/td>\nPanGreen\u2122 Universal SYBR\u00ae Green Mastermix<\/td>\n100 reactions
\n(20 \u03bcl vol)<\/td>\n<\/tr>\n
PDP01-0100<\/td>\nPlasmid miniPREP Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDP02-0020<\/td>\nPlasmid midiPREP Kit<\/td>\n20 rxns<\/td>\n<\/tr>\n
PDP03-0010<\/td>\nPlasmid maxiPREP Kit<\/td>\n10 rxns<\/td>\n<\/tr>\n
PDC01-0100<\/td>\nPCR Clean-Up & Gel Extraction Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC02-0100<\/td>\nGenomic DNA isolation dual kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC05-0100<\/td>\nDual Genomic DNA Isolation Kit (Plant)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC06-0100<\/td>\nDual Genomic DNA Isolation Kit(Tissue)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC09-0100<\/td>\nGenomic DNA Isolation Kit (Blood\/Cultured Cell\/Fungus)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC10-0100<\/td>\nGenomic DNA Isolation Kit (Plant)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC11-0100<\/td>\nGenomic DNA Isolation Kit (Tissue)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC12-0100<\/td>\nGenomic DNA Isolation Kit (Paraffin-embedded tissue)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDM01-0100<\/td>\nMbead Buffy Coat Genomic DNA Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDM02-0100<\/td>\nMbead Tissue Genomic DNA Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDM03-0100<\/td>\nMbead Bacteria Genomic DNA Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDM05-0100<\/td>\nMbead Plant Genomic DNA Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDM06-0100<\/td>\nMbead Blood\/Cell Genomic DNA Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDR01-0100<\/td>\nGenomic DNA Isolation Reagent<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDR02-0100<\/td>\nPlant Genomic DNA Isolation Reagent<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDR05-0100<\/td>\nGenomic DNA Isolation Reagent Kit(Blood\/Cultured Cell\/Tissue)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC04-0100<\/td>\nTotal RNA Isolation Kit (Blood\/Cultured Cell\/Fungus)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC07-0100<\/td>\nTotal RNA Isolation Kit (Plant)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC08-0100<\/td>\nTotal RNA Isolation Kit(Tissue)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDR03-0100<\/td>\nTotal RNA Isolation Reagent<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDR04-0100<\/td>\nPlant Total RNA Isolation Reagent<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC03-0100<\/td>\nVirus Nucleic Acid Isolation Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDM04-0100<\/td>\nMbead Virus Genomic Nucleic Acid Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
QP019M-0050<\/td>\nCOVID-19 Mutation RT-qPCR Detection Kit (Alpha, Delta, Kappa)<\/td>\n50 rxns<\/td>\n<\/tr>\n
QP019T-0100<\/td>\nCOVID-19 RT-qPCR Detection Kit Plus (TFDA)<\/td>\n100 rxns<\/td>\n<\/tr>\n
QP019-0100<\/td>\nSARS-CoV-2\/COVID-19 RT-qPCR Detection Kit (RUO)<\/td>\n100 rxns<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

PanProbes\u2122 Universal qPCR\u9810\u6df7\u6db2MasterMix| Bio-Helix \u8ca8\u865fQPD0 […]<\/p>\n","protected":false},"author":3,"featured_media":42593,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5372],"tags":[5769],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42591"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=42591"}],"version-history":[{"count":5,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42591\/revisions"}],"predecessor-version":[{"id":43311,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42591\/revisions\/43311"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/42593"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=42591"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=42591"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=42591"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}