{"id":42416,"date":"2023-05-17T11:05:27","date_gmt":"2023-05-17T03:05:27","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=42416"},"modified":"2023-06-11T14:27:24","modified_gmt":"2023-06-11T06:27:24","slug":"genomic-dna-isolation-dual-kit-column-based-bio-helix-%e8%b2%a8%e8%99%9fpdc02-0100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=42416","title":{"rendered":"\u57fa\u56e0\u7d44DNA Isolation Dual Kit (Column Based) | Bio-Helix \u8ca8\u865fPDC02-0100"},"content":{"rendered":"

\u57fa\u56e0\u7d44Genomic DNA Isolation Dual Kit (Column Based)| Bio-Helix \u8ca8\u865fPDC02-0100<\/strong><\/a><\/span><\/h4>\n

Genomic DNA Isolation Dual Kit (Column Based)<\/p>\n

\u7522\u54c1\u63cf\u8ff0:<\/strong><\/span><\/p>\n

DUAL \u57fa\u56e0\u7d44 DNA \u5206\u96e2\u8a66\u5291\u76d2\uff08\u8840\u6db2\/\u57f9\u990a\u7d30\u80de\/\u771f\u83cc\uff09\u7d50\u5408\u4e86\u8a66\u5291\u7cfb\u7d71( reagent system)\u548c\u96e2\u5fc3\u67f1\u7cfb\u7d71(spin column system)\u3002\u8a72\u8a66\u5291\u76d2\u5c08\u70ba\u5f9e\u5168\u8840( whole blood)\u3001\u51b7\u51cd\u8840\u6db2(frozen blood)\u3001\u8840\u6c89\u68d5\u9ec3\u5c64(buffy coat)\u3001\u57f9\u990a\u7684\u52d5\u7269\/\u7d30\u83cc\u7d30\u80de\u548c\u771f\u83cc\u4e2d\u5206\u96e2\u57fa\u56e0\u7d44 DNA \u800c\u8a2d\u8a08\u3002\u9019\u7a2e\u7368\u7279\u7684\u8a66\u5291\u7cfb\u7d71\u78ba\u4fdd\u4e86\u5f9e\u6a23\u54c1\u4e2d\u7372\u5f97\u7684\u7e3d DNA( total DNA) \u7522\u91cf\u9ad8\u3001\u8cea\u91cf\u597d\u3002\u96e2\u5fc3\u67f1\u7cfb\u7d71\u8a2d\u8a08\u7528\u65bc\u7d14\u5316\u6216\u6fc3\u7e2e\u5148\u524d\u5df2\u7528\u8a66\u5291\u5206\u96e2\u7684 DNA \u7522\u7269\u3002\u7121\u9700\u82ef\u915a\/\u6c2f\u4eff\u8403\u53d6( phenol\/ chloroform extraction)\uff0c\u6574\u500b\u904e\u7a0b\u53ef\u5728 1 \u5c0f\u6642\u5167\u5b8c\u6210\u3002\u7d14\u5316\u7684 DNA \u9069\u7528\u65bc PCR \u6216\u5176\u4ed6\u9176\u4fc3\u53cd\u61c9\u3002<\/p>\n


\nSample<\/strong><\/p>\n

Up to 300 \u03bcl of the whole blood
\nUp to 200 \u03bcl of the frozen blood
\nUp to 200 \u03bcl of the buffy coat
\nCultured animal cells (up to 1 x10^7<\/sup>)
\nCultured bacterial cells (up to 1 x10^9<\/sup>)
\nFungus cells (up to 5 x 10^7<\/sup>)<\/p>\n

Format<\/strong>
\nReagent and spin column<\/p>\n

Yield<\/strong>
\nUp to 50 \u03bcg<\/p>\n

Operation time<\/strong>
\nWithin 60 minutes<\/p>\n

Elution volume<\/strong>
\n50\u223c200 \u03bcl<\/p>\n

<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

\u258dFresh Whole Blood or Buffy Coat<\/strong><\/span><\/p>\n

Reagent System Protocol<\/strong><\/p>\n

Step 1 – Sample Cells Harvesting<\/strong>
\n1. Collect blood in the EDTA-Na2<\/sub>\u00a0treated collection tubes (or other anticoagulant mixtures).
\n2. Transfer up to 300 \u00b5l of the blood or 200 \u00b5l of buffy coat to a sterile 1.5 ml microcentrifuge tube.
\n3. Add\u00a0900 \u00b5l of the Buffer RL and mix by inversion.
\n4. Incubate the tube at the room temperature for 10 minutes (invert twice during incubation).
\n5. Centrifuge at 4,000 x g for 5 minutes.
\n6. Remove the supernatant completely and resuspend the cells in 50 \u00b5l of the Buffer RL by pipetting the pellet.<\/p>\n

Step 2 – Lysis<\/strong>
\n1. Add\u00a0300 \u00b5l of the Buffer CL to the resuspended cells from Step 1 and mix by vortex.
\n2. Incubate at 60\u00b0C for 10 minutes or until the sample lysate is clear. During the incubation,\u00a0invert the tube every 3 minutes.
\nOptional Step: RNA Degradation (If RNA-free genomic DNA is required, perform this optional step.)
\n3. Add\u00a05 \u00b5l of RNase A (10 mg\/ml) to the sample lysate and mix by vortex. Incubate at room\u00a0temperature for 5 minutes.<\/p>\n

Step 3 – Protein Removal<\/strong>
\n1. Add 100 \u00b5l of the Buffer PO to the sample lysate and vortex immediately for 10 seconds.
\n2. Incubate on ice for 5 minutes.
\n3. Centrifuge at 14-16,000 x g for 3 minutes.
\n4. Transfer the supernatant to a clean 1.5 ml microcentrifuge tube.
\nSwitch Step
\n\u25c6 If more pure DNA is required, please switch to Column System (DNA Pure) Protocol.<\/p>\n

Step 4 – DNA Precipitation<\/strong>
\n1. Add 300 \u00b5l of Isopropanol to the sample from the Step 3 and mix well by inverting 20 times.
\n2. Centrifuge at 14-16,000 x g for 5 minutes.
\n3. Discard the supernatant and add 300 \u00b5l of 70% ethanol to wash the pellet.
\n4. Centrifuge at 14-16,000 x g for 3 minutes.
\n5. Discard the supernatant and air-dry the pellet for 10 minutes.<\/p>\n

Step 5 DNA Rehydration<\/strong>
\n1. Add\u00a050-100 \u00b5l of the Buffer E and incubate at 60\u00b0C for 5-10 minutes to dissolve the DNA\u00a0pellet. During the incubation, tap the bottom of the tube to promote DNA rehydration.<\/p>\n

 <\/p>\n

Column System (DNA Pure) Protocol<\/strong>
\n* Add 60ml of the absolute ethanol to the Buffer W2 prior to initial use.
\n* Pre-heat the Buffer E to 60\u00b0C prior to use.
\nStep 1 – Sample Preparation<\/strong>
\n1. Add 400 \u00b5l of the Buffer BD to the sample from Step 3 Protein Removal and shake\u00a0vigorously.<\/p>\n

Step 2 – DNA Binding<\/strong>
\n1. Place a DG Column in a 2 ml Collection Tube.
\n2. Transfer the sample mixture from the previous step to the DG Column.
\n3. Centrifuge at 14-16,000 x g for 30 seconds.
\n4. Discard the flow-through and place the DG Column back in the same Collection Tube.<\/p>\n

Step 3 – Wash<\/strong>
\n1. Add 400 \u00b5l of the Buffer W1 into the DG Column.
\n2. Centrifuge at 14,000 x g for 30 seconds.
\n3. Discard the flow-through and place the DG Column back into the same Collection tube.
\n4. Add 600 \u00b5l of the Buffer W2 (Ethanol added) into the DG Column.
\n5. Centrifuge at 14,000 x g for 30 seconds.
\n6. Discard the flow-through and place the DG Column back into the same Collection tube.
\n7. Centrifuge at 14,000 x g again for 2 minutes to remove residual Buffer W2.<\/p>\n

Step 4 – DNA Elution<\/strong>
\n1. Place the dried DG column in a clean 1.5 ml microcentrifuge tube.
\n2. Add 50-200 \u00b5l of Pre-Heated Buffer E or TE (not provided) into the center of the column matrix.
\n3. Let it stand at 60\u00b0C for 5 minutes.
\n4. Centrifuge for 2 minutes at 14-16,000 x g to elute the purified DNA.<\/p>\n

\u258dCultured Mammalian Cells<\/strong><\/span><\/p>\n

Reagent System Protocol<\/strong><\/p>\n

Step 1 – Sample Cells Harvesting<\/strong>
\n1. Transfer cultured mammalian cells (up to 10^7<\/sup>) to a sterile 1.5 ml microcentrifuge tube.
\n2. Centrifuge at 6,000 x g for 1 minute.
\n3. Remove the supernatant completely and resuspend the cells in 50 \u00b5l of the Buffer RL by\u00a0pipetting the pellet.<\/p>\n

Step 2 – Lysis<\/strong>
\n1. Add 300 \u00b5l of the Buffer CL to the resuspended cells from Step 1 and mix by vortex.
\n2. Incubate at 60\u00b0C for 10 minutes or until the sample lysate is clear. During the incubation, invert the tube every 3 minutes.
\nOptional Step: RNA Degradation (If RNA-free genomic DNA is required, perform this optional step.)
\n3. Add 5 \u00b5l of RNase A (10 mg\/ml) to the sample lysate and mix by vortex. Incubate at room temperature for 5 minutes.<\/p>\n

Step 3 – Protein Removal<\/strong>
\n1. Add 100 \u00b5l of the Buffer PO to the sample lysate and vortex immediately for 10 seconds.
\n2. Incubate on ice for 5 minutes.
\n3. Centrifuge at 14-16,000 x g for 3 minutes.
\n4. Transfer the supernatant to a clean 1.5 ml microcentrifuge tube.
\nSwitch Step
\n\u25c6If more pure DNA is required, please switch to Column System (DNA Pure) Protocol.<\/p>\n

Step 4 – DNA Precipitation<\/strong>
\n1. Add 300 \u00b5l of Isopropanol to the sample from the Step 3 and mix well by inverting 20 times.
\n2. Centrifuge at 14-16,000 x g for 5 minutes.
\n3. Discard the supernatant and add 300 \u00b5l of 70% ethanol to wash the pellet.
\n4. Centrifuge at 14-16,000 x g for 3 minutes.
\n5. Discard the supernatant and air-dry the pellet for 10 minutes.<\/p>\n

Step 5 – DNA Rehydration<\/strong>
\n1. Add 50-100 \u00b5l of the Buffer E and incubate at 60\u00b0C for 5-10 minutes to dissolve the DNA
\npellet. During the incubation, tap the bottom of the tube to promote DNA rehydration.<\/p>\n

Column System (DNA Pure) Protocol<\/strong>
\n* Add 60ml of the absolute ethanol to the Buffer W2 prior to initial use.
\n* Pre-heat the Buffer E to 60\u00b0C prior to use.<\/p>\n

Step 1 – Sample Preparation<\/strong>
\n1. Add 400 \u00b5l of the Buffer BD to the sample from Step 3 Protein Removal and shake\u00a0vigorously.<\/p>\n

Step 2 – DNA Binding<\/strong>
\n1. Place a DG Column in a 2 ml Collection Tube.
\n2. Transfer the sample mixture from the previous step to the DG Column.
\n3. Centrifuge at 14-16,000 x g for 30 seconds.
\n4. Discard the flow-through and place the DG Column back in the same Collection Tube.<\/p>\n

Step 3 – Wash<\/strong>
\n1. Add 400 \u00b5l of the Buffer W1 into the DG Column.
\n2. Centrifuge at 14,000 x g for 30 seconds.
\n3. Discard the flow-through and place the DG Column back into the same Collection tube.
\n4. Add 600 \u00b5l of the Buffer W2 (Ethanol added) into the DG Column.
\n5. Centrifuge at 14,000 x g for 30 seconds.
\n6. Discard the flow-through and place the DG Column back into the same Collection tube.
\n7. Centrifuge at 14,000 x g again for 2 minutes to remove residual Buffer W2.<\/p>\n

Step 4 – DNA Elution<\/strong>
\n1. Place the dried DG column in a clean 1.5 ml microcentrifuge tube.
\n2. Add 50-200 \u00b5l of Pre-Heated Buffer E or TE (not provided) into the center of the column\u00a0matrix.
\n3. Let it stand at 60\u00b0C for 5 minutes.
\n4. Centrifuge for 2 minutes at 14-16,000 x g to elute the purified DNA.<\/p>\n

 <\/p>\n

\u258dGram-Negative Bacterial Cells<\/strong><\/span><\/p>\n

Reagent System Protocol<\/strong><\/p>\n

Step 1 – Sample Cells Harvesting<\/strong>
\n1. Transfer cultured bacterial cells (up to 10^9<\/sup>) to a sterile 1.5 ml microcentrifuge tube.
\n2. Centrifuge at 12,000 x g for 1 minute.
\n3. Remove the supernatant completely and resuspend the cells in 50 \u00b5l of the Buffer RL by pipetting the pellet.<\/p>\n

Step 2 – Lysis<\/strong>
\n1. Add 300 \u00b5l of the Buffer CL to the resuspended cells from Step 1 and mix by vortex.
\n2. Incubate at 60\u00b0C for 10 minutes or until the sample lysate is clear. During the incubation,\u00a0invert the tube every 3 minutes.
\nOptional Step: RNA Degradation (If RNA-free genomic DNA is required, perform this optional step.)
\n3. Add 5 \u00b5l of RNase A (10 mg\/ml) to the sample lysate and mix by vortex. Incubate at room temperature for 5 minutes.<\/p>\n

Step 3 – Protein Removal<\/strong>
\n1. Add 100 \u00b5l of the Buffer PO to the sample lysate and vortex immediately for 10 seconds.
\n2. Incubate on ice for 5 minutes.
\n3. Centrifuge at 14-16,000 x g for 3 minutes.
\n4. Transfer the supernatant to a clean 1.5 ml microcentrifuge tube.
\nSwitch Step
\n\u25c6If more pure DNA is required, please switch to Column System (DNA Pure) Protocol.<\/p>\n

Step 4 – DNA Precipitation<\/strong>
\n1. Add 300 \u00b5l of Isopropanol to the sample from the Step 3 and mix well by inverting 20 times.
\n2. Centrifuge at 14-16,000 x g for 5 minutes.
\n3. Discard the supernatant and add 300 \u00b5l of 70% ethanol to wash the pellet.
\n4. Centrifuge at 14-16,000 x g for 3 minutes.
\n5. Discard the supernatant and air-dry the pellet for 10 minutes.<\/p>\n

Step 5 – DNA Rehydration<\/strong>
\n1. Add 50-100 \u00b5l of the Buffer E and incubate at 60\u00b0C for 5-10 minutes to dissolve the DNA pellet. During the incubation, tap the bottom of the tube to promote DNA rehydration.<\/p>\n

 <\/p>\n

Column System (DNA Pure) Protocol<\/strong><\/p>\n

* Add 60ml of the absolute ethanol to the Buffer W2 prior to initial use.
\n* Pre-heat the Buffer E to 60\u00b0C prior to use.<\/p>\n

Step 1 – Sample Preparation<\/strong>
\n1. Add 400 \u00b5l of the Buffer BD to the sample from Step 3 Protein Removal and shake vigorously.<\/p>\n

Step 2 – DNA Binding<\/strong>
\n1. Place a DG Column in a 2 ml Collection Tube.
\n2. Transfer the sample mixture from the previous step to the DG Column.
\n3. Centrifuge at 14-16,000 x g for 30 seconds.
\n4. Discard the flow-through and place the DG Column back in the 2 ml Collection Tube.<\/p>\n

Step 3 – Wash<\/strong>
\n1. Add 400 \u00b5l of the Buffer W1 into the DG Column.
\n2. Centrifuge at 14,000 x g for 30 seconds.
\n3. Discard the flow-through and place the DG Column back into the same Collection tube.
\n4. Add 600 \u00b5l of the Buffer W2 (Ethanol added) into the DG Column.
\n5. Centrifuge at 14,000 x g for 30 seconds.
\n6. Discard the flow-through and place the DG Column back into the same Collection tube.
\n7. Centrifuge at 14,000 x g again for 2 minutes to remove residual Buffer W2.<\/p>\n

Step 4 – DNA Elution<\/strong>
\n1. Place the dried DG column in a clean 1.5 ml microcentrifuge tube.
\n2. Add 50-200 \u00b5l of Pre-Heated Buffer E or TE (not provided) into the center of the column matrix.
\n3. Let it stand at 60\u00b0C for 5 minutes.
\n4. Centrifuge for 2 minutes at 14-16,000 x g to elute the purified DNA.<\/p>\n

\u258dGram-Postive Bacterial Cells<\/strong><\/span><\/p>\n

Reagent System Protocol<\/strong><\/p>\n

Step 1 – Sample Cells Harvesting<\/strong>
\n1. Transfer cultured bacterial cells (up to 10^9<\/sup>) to a sterile 1.5 ml microcentrifuge tube.
\n2. Centrifuge at 12,000 x g for 1 minute.
\n3. Remove the supernatant completely and resuspend the cells in 100 \u00b5l of lysozyme Buffer by pipetting the pellet.
\n4. Incubate at room temperature for 20 minutes.
\nStep 2 – Lysis<\/strong><\/p>\n

1. Add 300 \u00b5l of the Buffer CL to the resuspended cells from Step 1 and mix by vortex.
\n2. Incubate at 60\u00b0C for 10 minutes or until the sample lysate is clear. During the incubation,\u00a0invert the tube every 3 minutes.
\nOptional Step: RNA Degradation (If RNA-free genomic DNA is required, perform this optional step.)
\n3. Add 5 \u00b5l of RNase A (10 mg\/ml) to the sample lysate and mix by vortex. Incubate at room\u00a0temperature for 5 minutes.<\/p>\n

Step 3 – Protein Removal<\/strong>
\n1. Add 100 \u00b5l of the Buffer PO to the sample lysate and vortex immediately for 10 seconds.
\n2. Incubate on ice for 5 minutes.
\n3. Centrifuge at 14-16,000 x g for 3 minutes.
\n4. Transfer the supernatant to a clean 1.5 ml microcentrifuge tube.
\nSwitch Step
\n\u25c6If more pure DNA is required, please switch to Column System (DNA Pure) Protocol.<\/p>\n

Step 4 – DNA Precipitation<\/strong>
\n1. Add 300 \u00b5l of Isopropanol to the sample from the Step 3 and mix well by inverting 20 times.
\n2. Centrifuge at 14-16,000 x g for 5 minutes.
\n3. Discard the supernatant and add 300 \u00b5l of 70% ethanol to wash the pellet.
\n4. Centrifuge at 14-16,000 x g for 3 minutes.
\n5. Discard the supernatant and air-dry the pellet for 10 minutes.<\/p>\n

Step 5 – DNA Rehydration<\/strong>
\n1. Add 50-100 \u00b5l of the Buffer E and incubate at 60\u00b0C for 5-10 minutes to dissolve the DNA\u00a0pellet. During the incubation, tap the bottom of the tube to promote DNA rehydration.<\/p>\n

 <\/p>\n

Column System (DNA Pure) Protocol<\/strong><\/p>\n

* Add 60ml of the absolute ethanol to the Buffer W2 prior to initial use.
\n* Pre-heat the Buffer E to 60\u00b0C prior to use.<\/p>\n

Step 1 – Sample Preparation<\/strong>
\n1. Add 400 \u00b5l of the Buffer BD to the sample from Step 3 Protein Removal and shake vigorously.<\/p>\n

Step 2 – DNA Binding<\/strong>
\n1. Place a DG Column in a 2 ml Collection Tube.
\n2. Transfer the sample mixture from the previous step to the DG Column.
\n3. Centrifuge at 14-16,000 x g for 30 seconds.
\n4. Discard the flow-through and place the DG Column back in the 2 ml Collection Tube.<\/p>\n

Step 3 – Wash<\/strong>
\n1. Add 400 \u00b5l of the Buffer W1 into the DG Column.
\n2. Centrifuge at 14,000 x g for 30 seconds.
\n3. Discard the flow-through and place the DG Column back into the same Collection tube.
\n4. Add 600 \u00b5l of the Buffer W2 (Ethanol added) into the DG Column.
\n5. Centrifuge at 14,000 x g for 30 seconds.
\n6. Discard the flow-through and place the DG Column back into the same Collection tube.
\n7. Centrifuge at 14,000 x g again for 2 minutes to remove residual Buffer W2.<\/p>\n

Step 4 – DNA Elution<\/strong>
\n1. Place the dried DG column in a clean 1.5 ml microcentrifuge tube.
\n2. Add 50-200 \u00b5l of Pre-Heated Buffer E or TE (not provided) into the center of the column matrix.
\n3. Let it stand at 60\u00b0C for 5 minutes.
\n4. Centrifuge for 2 minutes at 14-16,000 x g to elute the purified DNA.<\/p>\n

\u258dFungus Cells<\/strong><\/span><\/p>\n

Reagent System Protocol<\/strong><\/p>\n

Step 1 – Sample Cells Harvesting<\/strong>
\n1. Transfer fungus cells (up to 10^8<\/sup>) to a sterile 1.5 ml microcentrifuge tube.
\n2. Centrifuge at 6,000 x g for 5 minutes.
\n3. Remove the supernatant completely and resuspend the cells in 600 \u00b5l of sorbitol Buffer by pipetting the pellet.
\n4. Add 200 U of lyticase or zymolase. Incubate at 30\u00b0C for 30 minutes.
\n5. Centrifuge the mixture for 10 minutes at 2,000 x g to harvest the spheroplast.
\n6. Remove the supernatant completely and resuspend the cells in 50 \u00b5l of the Buffer RL by\u00a0pipetting the pellet.
\nStep 2 – Lysis<\/strong>
\n1. Add 300 \u00b5l of the Buffer CL to the resuspended cells from Step 1 and mix by vortex.
\n2. Incubate at 60\u00b0C for 10 minutes or until the sample lysate is clear. During the incubation,\u00a0invert the tube every 3 minutes.
\nOptional Step: RNA Degradation (If RNA-free genomic DNA is required, perform this optional step.)
\n3. Add 5 \u00b5l of RNase A (10 mg\/ml) to the sample lysate and mix by vortex. Incubate at room\u00a0temperature for 5 minutes.
\nStep 3 – Protein Removal<\/strong>
\n1. Add 100 \u00b5l of the Buffer PO to the sample lysate and vortex immediately for 10 seconds.
\n2. Incubate on ice for 5 minutes.
\n3. Centrifuge at 14-16,000 x g for 3 minutes.
\n4. Transfer the supernatant to a clean 1.5 ml microcentrifuge tube.
\nSwitch Step
\n\u25c6If more pure DNA is required, please switch to Column System (DNA Pure) Protocol.
\nStep 4 – DNA Precipitation<\/strong>
\n1. Add 300 \u00b5l of Isopropanol to the sample from the Step 3 and mix well by inverting 20 times.
\n2. Centrifuge at 14-16,000 x g for 5 minutes.
\n3. Discard the supernatant and add 300 \u00b5l of 70% ethanol to wash the pellet.
\n4. Centrifuge at 14-16,000 x g for 3 minutes.
\n5. Discard the supernatant and air-dry the pellet for 10 minutes.<\/p>\n

Step 5 – DNA Rehydration<\/strong>
\n1. Add 50-100 \u00b5l of the Buffer E and incubate at 60\u00b0C for 5-10 minutes to dissolve the DNA\u00a0pellet. During the incubation, tap the bottom of the tube to promote DNA rehydration.<\/p>\n

 <\/p>\n

Column System (DNA Pure) Protocol<\/strong><\/p>\n

* Add 60ml of the absolute ethanol to the Buffer W2 prior to initial use.
\n* Pre-heat the Buffer E to 60\u00b0C prior to use.<\/p>\n

Step 1 – Sample Preparation<\/strong>
\n1. Add 400 \u00b5l of the Buffer BD to the sample from Step 3 Protein Removal and shake vigorously.<\/p>\n

Step 2 – DNA Binding<\/strong>
\n1. Place a DG Column in a 2 ml Collection Tube.
\n2. Transfer the sample mixture from the previous step to the DG Column.
\n3. Centrifuge at 14-16,000 x g for 30 seconds.
\n4. Discard the flow-through and place the DG Column back in the 2 ml Collection Tube.<\/p>\n

Step 3 – Wash<\/strong>
\n1. Add 400 \u00b5l of the Buffer W1 into the DG Column.
\n2. Centrifuge at 14,000 x g for 30 seconds.
\n3. Discard the flow-through and place the DG Column back into the same Collection tube.
\n4. Add 600 \u00b5l of the Buffer W2 (Ethanol added) into the DG Column.
\n5. Centrifuge at 14,000 x g for 30 seconds.
\n6. Discard the flow-through and place the DG Column back into the same Collection tube.
\n7. Centrifuge at 14,000 x g again for 2 minutes to remove residual Buffer W2.<\/p>\n

Step 4 – DNA Elution<\/strong>
\n1. Place the dried DG column in a clean 1.5 ml microcentrifuge tube.
\n2. Add 50-200 \u00b5l of Pre-Heated Buffer E or TE (not provided) into the center of the column matrix.
\n3. Let it stand at 60\u00b0C for 5 minutes.
\n4. Centrifuge for 2 minutes at 14-16,000 x g to elute the purified DNA.<\/p>\n

\"\"<\/p>\n

PCR\u6df7\u5408\u6db2<\/a>\u00a0|\u00a0<\/span>100bp-10kb DNA Ladder\u00a0<\/a>\u00a0|<\/span>\u00a0\u74ca\u8102\u81a0\u9ad4\u7c89Agarose<\/a>\u00a0|<\/span>\u00a0\u00a0DNA\u6838\u9178\u67d3\u5291<\/a>\u00a0|<\/span>\u00a0\u85cd\u5149\u89c0\u5bdf\u7bb1(\u542b\u767d\u5149)<\/a>\u00a0|<\/span>\u00a0Mupid-2\u00a0\u6c34\u5e73\u96fb\u6cf3\u69fd<\/a>\u00a0|<\/span><\/span><\/strong><\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716<\/p>\n

\"BIO-HIDEX\"<\/p>\n

bio-helix.com<\/p>\n

Bio-Helix\u6210\u7acb\u65bc2007\u5e74\uff0c\u5c08\u6ce8\u65bc\u70ba\u5168\u7403\u751f\u547d\u79d1\u5b78\u7814\u7a76\u4eba\u54e1\u63d0\u4f9b\u6700\u597d\u7684\u751f\u7269\u8a66\u5291\u3002\u5305\u62ec DNA \u6a19\u8a18\u7269\u3001\u86cb\u767d\u8cea\u6a19\u8a18\u7269\u3001\u9810\u88fd\u86cb\u767d\u8cea\u51dd\u81a0\u3001\u6838\u9178\u7d14\u5316\u3001PCR\u8a66\u5291\u7b49\u3002\u8207\u4e16\u754c\u5404\u5730\u5be6\u9a57\u5ba4\u7684\u79d1\u5b78\u5bb6\u5011\u4e00\u8d77\u52aa\u529b\uff0c\u4e0d\u65b7\u63d0\u9ad8\u8cea\u91cf\uff0c\u958b\u59cb\u8d0f\u5f97\u5ba2\u6236\u7684\u4fe1\u4efb\u548c\u8a55\u50f9\u3002\u54c1\u724c\u904d\u5e03\u56db\u5341\u591a\u500b\u570b\u5bb6\uff0c\u5df2\u6210\u70ba\u751f\u547d\u79d1\u5b78\u5be6\u9a57\u5ba4\u503c\u5f97\u4fe1\u8cf4\u548c\u6b61\u8fce\u7684\u8a66\u5291\u54c1\u724c\u3002Bio-Helix\u500b\u9858\u666f\uff0c\u5c31\u662f\u6709\u4e00\u5929\u6211\u5011\u7684\u58fd\u547d\u53ef\u4ee5\u5ef6\u9577\uff1b\u6240\u6709\u75be\u75c5\u7684\u65e9\u671f\u767c\u73fe\u548c\u96a8\u5f8c\u7684\u9069\u7576\u6cbb\u7642\u90fd\u53ef\u4ee5\u63d0\u9ad8\u751f\u6d3b\u8cea\u91cf\u3002\u4e2d\u570b\u53e4\u4eba\u8a8d\u70ba\uff0c\u826f\u91ab\u6cbb\u672a\u75c5\uff0c\u6cbb\u672a\u75c5\u3002\u5728 Bio-Helix \u4e2d\uff0c\u5275\u5efa\u4e86\u4e00\u500b\u65b0\u54c1\u724c LifeDirex \u4f86\u5be6\u73fe\u9019\u4e00\u9858\u666f\u3002 LifeDirex \u5c07\u5c08\u6ce8\u65bc\u70ba\u8a3a\u65b7\u5de5\u5177\u63d0\u4f9b\u6700\u597d\u548c\u6700\u5177\u5275\u65b0\u6027\u7684\u8a66\u5291\uff0c\u4ee5\u4fc3\u9032\u76e1\u53ef\u80fd\u65e9\u5730\u767c\u73fe\u75be\u75c5\u3002Bio-Helix\u4f7f\u547d\u662f\u63d0\u4f9b\u6700\u512a\u8cea\u7684\u7522\u54c1\u4e26\u958b\u767c\u65b0\u6280\u8853\uff0c\u5e6b\u52a9\u7814\u7a76\u4eba\u54e1\u627e\u5230\u5c0b\u6c42\u79d1\u5b78\u6210\u5c31\u7684\u89e3\u6c7a\u65b9\u6848\u3002\u6b64\u5916\uff0cBio-Helix\u8a8d\u70ba\u73fe\u5728\u662f\u9032\u5165\u9ad4\u5916\u8a3a\u65b7\u8a2d\u5099\uff08IVDD\uff09\u5e02\u5834\u7684\u6700\u4f73\u6642\u6a5f\u3002\u6211\u5011\u7684\u76ee\u6a19\u662f\u6210\u70ba\u7814\u7a76\u5be6\u9a57\u5ba4\u548cIVDD\u7684\u6700\u4f73\u89e3\u6c7a\u65b9\u6848\u63d0\u4f9b\u5546\u3002<\/p>\n

\u5728\u6539\u5584\u751f\u6d3b\u8cea\u91cf\u548c\u5be6\u73fe\u79d1\u5b78\u5353\u8d8a\u7684\u9858\u671b\u7684\u9a45\u4f7f\u4e0b\uff0cBio-Helix\u4ee5\u6700\u9ad8\u7684\u8aa0\u4fe1\u6a19\u6e96\u904b\u71df\u3002\u9084\u5c07\u9019\u7a2e\u7cbe\u795e\u5ef6\u4f38\u5230\u6211\u5011\u7684\u670d\u52d9\u548c\u5168\u7403\u50f9\u503c\u4e2d\uff0c\u4ee5\u63d0\u4f9b\u7d66\u4e16\u754c\u5404\u5730\u7684\u5ba2\u6236\u3002\u591a\u6a23\u5316\u7684\u54e1\u5de5\u968a\u4f0d\u548c\u5354\u4f5c\u7db2\u7d61\u5f62\u6210\u4e86\u516c\u53f8\u6587\u5316\u7684\u5b8c\u7f8e\u7d50\u5408\u3002 Bio-Helix \u4e0d\u50c5\u662f\u4e00\u5bb6\u6a5f\u6703\u5747\u7b49\u7684\u516c\u53f8\uff0c\u800c\u4e14\u9084\u52aa\u529b\u63d0\u4f9b\u53cb\u597d\u548c\u6210\u9577\u7684\u74b0\u5883\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
CAT#<\/td>\nNAME<\/td>\nSize<\/td>\n<\/tr>\n
BP001CU<\/td>\nBluPAD, LED Transilluminator, with mini Darkroom
\n(CE\/ETL, Patent No: M543442)<\/td>\n		Set<\/td>\n<\/tr>\n
PMI09-0500<\/td>\nIRIS9 Plus Prestained Protein Ladder
\n(9 pre-stained bands, 15-170 kDa)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
PMI11-0500<\/td>\nIRIS11 Prestained Protein Ladder
\n(11 pre-stained bands, 3-260 kDa)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
PMB01-0500<\/td>\nBlu10 Plus Prestained Protein Ladder
\n(10 pre-stained bands, 6.5-270 kDa)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
PMB10-0500<\/td>\nBlu10 Prestained Protein Ladder
\n(10 pre-stained bands, 11-180 kDa)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
PMB11-0500<\/td>\nBlu11 Prestained Protein Ladder
\n(11 pre-stained bands, 10-180 kDa)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
PMB12-0500<\/td>\nBlu12 Prestained Protein Ladder
\n(12 pre-stained bands, 11-245 kDa)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
PMB13-0500<\/td>\nBlu13 Prestained Protein Ladder
\n(13 pre-stained bands, 5-245 kDa)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
PMU12-0500<\/td>\nUNveil Unstained Protein Ladder, Broad Range
\n(12 unstained bands, 10-200 kDa)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
CCH321-B100ML<\/td>\nUltraScence Pico Plus Western Substrate
\n(Patent No: US10,711,185)<\/td>\n		100 ml
\n(50ml+50ml)<\/td>\n<\/tr>\n
CCH345-B100ML<\/td>\nUltraScence Pico Ultra Western Substrate
\n(Patent No: US10,711,185)<\/td>\n		100 ml
\n(50ml+50ml)<\/td>\n<\/tr>\n
CCH365-B100ML<\/td>\nUltraScence Femto Western Substrate
\n(Patent No: US10,711,185)<\/td>\n		100 ml
\n(50ml+50ml)<\/td>\n<\/tr>\n
CCH375-B100ML<\/td>\nUltraScence Femto Plus Western Substrate
\n(Patent No: US10,711,185)<\/td>\n		100 ml
\n(50ml+50ml)<\/td>\n<\/tr>\n
PS002-B500ML<\/td>\nCOOMASSIEnano Protein Staining Solution, 500ml<\/td>\n500 ml<\/td>\n<\/tr>\n
PS003-B500ML<\/td>\nPonceau S Protein Staining Solution<\/td>\n500 ml<\/td>\n<\/tr>\n
BS001-B500ML<\/td>\nOneStep Blocker
\nWestern Blocking Solution and Signal Enhancer<\/td>\n		500 ml<\/td>\n<\/tr>\n
TM001-B200ML<\/td>\nTMB ELISA Substrate<\/td>\n100 ml
\n(50ml+50ml)<\/td>\n<\/tr>\n
DM001-R500<\/td>\nBH 100bp DNA Ladder RTU
\n(11 bands, 100-1,500bps)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
DM003-R500<\/td>\nBH 100bp DNA Ladder H3 RTU
\n(12 bands, 100-3,000bps)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
DM012-R500<\/td>\nBH 50bp DNA Ladder RTU
\n(17 bands, 50-1,500bps)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
DM015-R500<\/td>\nBH 1Kb Plus DNA Ladder RTU
\n(13 bands, 100-10,000bps)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
DMF12-0100<\/td>\nOmniMARK 100 DNA Ladder RTU
\n(12 bands, 100-3,000bps, fluorescent dye)<\/td>\n		600 \u03bcl<\/td>\n<\/tr>\n
DMF13-0100<\/td>\nOmniMARK 1K DNA Ladder RTU
\n(13 bands, 100-10,000bps, fluorescent dye)<\/td>\n		600 \u03bcl<\/td>\n<\/tr>\n
LD011-1000<\/td>\nPrime Juice Preloading Fluorescent Stain<\/td>\n1 ml<\/td>\n<\/tr>\n
LD003-0500<\/td>\nNovel Green Plus (20,000X) DNA Staining Reagent<\/td>\n500 \u03bcl<\/td>\n<\/tr>\n
AGT002-0500<\/td>\nAgarose Tablets, 110 pcs<\/td>\n0.5g x 110<\/td>\n<\/tr>\n
MB755-0100<\/td>\nAgarose Powder 100g -Molecular Biology Grade<\/td>\n100 g<\/td>\n<\/tr>\n
MB755-0500<\/td>\nAgarose Powder 500g -Molecular Biology Grade<\/td>\n500 g<\/td>\n<\/tr>\n
MB101-0500<\/td>\nTaq DNA Polymerase<\/td>\n500U\/100\u03bcl<\/td>\n<\/tr>\n
DP001-0100<\/td>\nnanoTaq\u2122 Hot-Start DNA Polymerase<\/td>\n100 rxns
\n500U\/100\u03bcl<\/td>\n<\/tr>\n
DN001-0250<\/td>\n100 mM dNTP Set<\/td>\n4 x 250\u03bcl<\/td>\n<\/tr>\n
DN0010<\/td>\n10 mM dNTP Mix<\/td>\n1 ml<\/td>\n<\/tr>\n
MB200-P100<\/td>\n2X PCR SuperMix<\/td>\n100 rxns
\n2 x 1.25mL<\/td>\n<\/tr>\n
MBA01-0100<\/td>\nOmniPCR Supermix w Fluorescent dye<\/td>\n100 rxns
\n(2.5ml)<\/td>\n<\/tr>\n
RK001-0050<\/td>\nRScript\u2122 cDNA Synthesis Kit<\/td>\n50 rxns<\/td>\n<\/tr>\n
RT001-0050<\/td>\nRScript\u2122 Reverse Transcriptase 10,000U<\/td>\n50 rxns
\n10,000U\/50\u03bcl<\/td>\n<\/tr>\n
RT001-0250<\/td>\nRScript\u2122 Reverse Transcriptase 50,000U<\/td>\n250 rxns
\n50,000U\/250\u03bcl<\/td>\n<\/tr>\n
RI001-0125<\/td>\nRIBOAssure\u2122 RNase Inhibitor 5,000U<\/td>\n125 rxns
\n5,000U\/125\u03bcl<\/td>\n<\/tr>\n
QPR01-0100<\/td>\nPanProbes\u2122 One-Step RT-qPCR Kit<\/td>\n100 reactions
\n(20 \u03bcl vol)<\/td>\n<\/tr>\n
QPD01-0100<\/td>\nPanProbes\u2122 Universal qPCR MasterMix<\/td>\n100 reactions
\n(20 \u03bcl vol)<\/td>\n<\/tr>\n
QSR01-0100<\/td>\nPanGreen\u2122 One-Step RT-qPCR Kit<\/td>\n100 reactions
\n(20 \u03bcl vol)<\/td>\n<\/tr>\n
QSD01-0100<\/td>\nPanGreen\u2122 Universal SYBR\u00ae Green Mastermix<\/td>\n100 reactions
\n(20 \u03bcl vol)<\/td>\n<\/tr>\n
PDP01-0100<\/td>\nPlasmid miniPREP Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDP02-0020<\/td>\nPlasmid midiPREP Kit<\/td>\n20 rxns<\/td>\n<\/tr>\n
PDP03-0010<\/td>\nPlasmid maxiPREP Kit<\/td>\n10 rxns<\/td>\n<\/tr>\n
PDC01-0100<\/td>\nPCR Clean-Up & Gel Extraction Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC02-0100<\/td>\nGenomic DNA isolation dual kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC05-0100<\/td>\nDual Genomic DNA Isolation Kit (Plant)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC06-0100<\/td>\nDual Genomic DNA Isolation Kit(Tissue)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC09-0100<\/td>\nGenomic DNA Isolation Kit (Blood\/Cultured Cell\/Fungus)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC10-0100<\/td>\nGenomic DNA Isolation Kit (Plant)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC11-0100<\/td>\nGenomic DNA Isolation Kit (Tissue)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC12-0100<\/td>\nGenomic DNA Isolation Kit (Paraffin-embedded tissue)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDM01-0100<\/td>\nMbead Buffy Coat Genomic DNA Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDM02-0100<\/td>\nMbead Tissue Genomic DNA Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDM03-0100<\/td>\nMbead Bacteria Genomic DNA Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDM05-0100<\/td>\nMbead Plant Genomic DNA Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDM06-0100<\/td>\nMbead Blood\/Cell Genomic DNA Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDR01-0100<\/td>\nGenomic DNA Isolation Reagent<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDR02-0100<\/td>\nPlant Genomic DNA Isolation Reagent<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDR05-0100<\/td>\nGenomic DNA Isolation Reagent Kit(Blood\/Cultured Cell\/Tissue)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC04-0100<\/td>\nTotal RNA Isolation Kit (Blood\/Cultured Cell\/Fungus)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC07-0100<\/td>\nTotal RNA Isolation Kit (Plant)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC08-0100<\/td>\nTotal RNA Isolation Kit(Tissue)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDR03-0100<\/td>\nTotal RNA Isolation Reagent<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDR04-0100<\/td>\nPlant Total RNA Isolation Reagent<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC03-0100<\/td>\nVirus Nucleic Acid Isolation Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDM04-0100<\/td>\nMbead Virus Genomic Nucleic Acid Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
QP019M-0050<\/td>\nCOVID-19 Mutation RT-qPCR Detection Kit (Alpha, Delta, Kappa)<\/td>\n50 rxns<\/td>\n<\/tr>\n
QP019T-0100<\/td>\nCOVID-19 RT-qPCR Detection Kit Plus (TFDA)<\/td>\n100 rxns<\/td>\n<\/tr>\n
QP019-0100<\/td>\nSARS-CoV-2\/COVID-19 RT-qPCR Detection Kit (RUO)<\/td>\n100 rxns<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

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