{"id":41647,"date":"2023-04-25T09:32:08","date_gmt":"2023-04-25T01:32:08","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=41647"},"modified":"2023-04-27T09:43:17","modified_gmt":"2023-04-27T01:43:17","slug":"%e6%8f%ad%e9%96%8b%e6%9c%aa%e6%9f%93%e8%89%b2%e8%9b%8b%e7%99%bd%e5%88%86%e5%ad%90%e9%87%8f%e6%a8%99%e6%ba%96%ef%bc%8810-to-200-kda%ef%bc%89-%e8%a6%8f%e6%a0%bc%ef%bc%9a500-%e5%be%ae%e5%8d%87bio-helix","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=41647","title":{"rendered":"\u672a\u67d3\u8272\u86cb\u767d\u5206\u5b50\u91cf\u6a19\u6e96\uff0810 to 200 kDa\uff09|Bio-Helix \u8ca8\u865fPMU12-0500"},"content":{"rendered":"

\u672a\u67d3\u8272\u86cb\u767d\u5206\u5b50\u91cf\u6a19\u6e96\uff0810 to 200 kDa\uff09|Bio-Helix \u8ca8\u865fPMU12-0500<\/strong><\/a><\/span><\/h4>\n

UNveil Unstained Protein Ladder\uff0810 to 200 kDa\uff09SIZE: 500 \u03bcl<\/span><\/p>\n

\u7522\u54c1\u63cf\u8ff0:<\/strong><\/span><\/p>\n

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The Unveil Unstained Protein Ladder is a mixture of\u00a012 unstained recombinant proteins<\/u>\u00a0covering a wide range of molecular weights from 10 to 200 kDa. The proteins resolve into clearly identifiable bands when\u00a0separated on the SDS-PAGE gel (MES buffer), with the intensified 25kDa and 85kDa protein bands serving as the two reference bands.\u00a0 The Unveil Unstained Protein Ladder is designed for monitoring protein\u00a0separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in the gel loading buffer and is ready to use.<\/p>\n

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3 \u03bcl or 5 \u03bcl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 \u03bcl per well for general Western transferring.<\/strong><\/span><\/h1>\n<\/div>\n<\/div>\n<\/div>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

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Storage Buffer\u00a0:\u00a0<\/strong><\/span>
\n20 mM Tris-phosphate(pH 7.5 at 25\u00b0C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v\/v) Glycerol.<\/p>\n

Quality Control\u00a0:\u00a0<\/strong><\/span>
\nUnder suggested conditions, Unveil Unstained Protein Ladder resolves 12 major bands in 4-12%Bis- Tris Gel(MES buffer) and after Western blotting to the nitrocellulose membrane.<\/p>\n

Storage :<\/strong><\/span>
\nStable for up to 2 weeks at 25\u00b0C.
\nStable for up to 3 months at 4\u00b0C.
\nStable for up to 24\u00a0months at \u00a0-20\u00b0C.<\/p>\n

Note. The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards; supplemental data should be considered for more accurate adjustment in different electrophoresis conditions.<\/p>\n

All products are for research use only.<\/p>\n

Caution :<\/strong><\/span>
\nNot intended for human or animal diagnostic or therapeutic uses.<\/p>\n<\/div>\n<\/div>\n<\/div>\n

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