{"id":41358,"date":"2023-04-20T14:39:15","date_gmt":"2023-04-20T06:39:15","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=41358"},"modified":"2025-10-30T14:01:43","modified_gmt":"2025-10-30T06:01:43","slug":"%e4%ba%ba%e9%a1%9e%e7%ab%af%e7%b2%92%e9%95%b7%e5%ba%a6%e5%ae%9a%e9%87%8f-qpcr-%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-elk-%e8%b2%a8%e8%99%9feq022","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=41358","title":{"rendered":"\u4eba\u985e\u7aef\u7c92\u9577\u5ea6\u5b9a\u91cf qPCR \u6aa2\u6e2c\u8a66\u5291\u76d2 | ELK \u8ca8\u865fEQ022"},"content":{"rendered":"<p id=\"tw-target-text\" class=\"tw-data-text tw-text-large tw-ta\" dir=\"ltr\" data-placeholder=\"\u7ffb\u8b6f\"><span style=\"color: #008080;\"><strong><a style=\"color: #008080;\" href=\"http:\/\/biopioneer.com.tw\/?p=41358\"><span style=\"font-size: 12pt;\">\u4eba\u985e\u7aef\u7c92\u9577\u5ea6\u5b9a\u91cf qPCR \u6aa2\u6e2c\u8a66\u5291\u76d2 | ELK \u8ca8\u865fEQ022<\/span><\/a><\/strong><\/span><\/p>\n<p>Human Telomere Length Quantification qPCR Assay Kit<\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>\u7522\u54c1\u63cf\u8ff0:\u00a0<\/strong><\/span><\/p>\n<p>\u7aef\u7c92\uff08Telomere\uff09\u662f\u4f4d\u65bc\u771f\u6838\u7d30\u80de\u67d3\u8272\u9ad4(eukaryotic cell chromosome)\u672b\u7aef\u7684\u4e00\u6bb5DNA\u5e8f\u5217(DNA sequence )\uff0c\u7531\u591a\u500b\u91cd\u8907\u6838\u82f7\u9178\u5143\u4ef6\uff08multiplerepeatingnucleotide elements, TTAGGG\uff09\u4e32\u806f\u7d44\u6210\u3002\u70ba\u975e\u8f49\u9304\u7684DNA(\u00a0non-transcribed DNA)\u63d0\u4f9b\u7de9\u885d\uff0c\u9084\u53ef\u4ee5\u4fdd\u8b77\u67d3\u8272\u9ad4(\u00a0chromosome)\u672b\u7aef\u4e0d\u767c\u751f\u878d\u5408\u548c\u9000\u5316\uff0c\u4fdd\u8b77\u67d3\u8272\u9ad4\u7d50\u69cb(chromosome structure )\u7684\u7a69\u5b9a\u6027\u548c\u907a\u50b3\u5b8c\u6574\u6027(geneticintegrity)\u3002\u7aef\u7c92\uff08Telomere\uff09\u662f\u4e00\u500b\u4eba\u8870\u8001\u901f\u5ea6\u6700\u91cd\u8981\u3001\u6700\u6e96\u78ba\u7684\u6307\u6a19\u3002\u5b83\u7684\u521d\u59cb\u9577\u5ea6\u662f\u7531\u907a\u50b3\u548c\u74b0\u5883\u56e0\u7d20\u6c7a\u5b9a\u7684\uff0c\u4e26\u4e14\u6703\u96a8\u8457\u6642\u9593\u7684\u63a8\u79fb\u800c\u6e1b\u5c11\u3002\u7814\u7a76\u8868\u660e\uff0c\u7aef\u7c92\uff08Telomere\uff09\u9577\u5ea6\u8207DNA\u4fee\u5fa9(DNA repair)\u3001\u8870\u8001(aging)\u3001\u7d30\u80de\u51cb\u4ea1(apoptosis)\u548c\u816b\u7624\u767c\u751f(tumorigenesis)\u5bc6\u5207\u76f8\u95dc\u3002\u56e0\u6b64\uff0c\u6e96\u78ba\u3001\u53ef\u91cd\u8907\u5730\u6e2c\u91cf\u7aef\u7c92\u9577\u5ea6\u5c0d\u7814\u7a76\u4eba\u54e1\u4f86\u8aaa\u5c24\u70ba\u91cd\u8981\u3002\u672c\u7522\u54c1\u4e3b\u8981\u63a1\u7528\u76f8\u5c0d\u5b9a\u91cfqPCR\u76f4\u63a5\u6bd4\u8f03\u6a23\u672c\u5e73\u5747\u7aef\u7c92\u9577\u5ea6(telomere length)\uff0c\u5373\u4ee5\u7aef\u7c92\u91cd\u8907\u5e8f\u5217\uff08telomererepeatsequence, Tel\uff09\u8207\u57fa\u56e0\u7d44\u55ae\u62f7\u8c9d\u57fa\u56e0(genome single copy gene. SCR\uff09\u62f7\u8c9d\u6578\uff08T\/S\uff09\u7684\u62f7\u8c9d\u6578\u6bd4\uff08T\/S\uff09\u4f5c\u70ba\u7aef\u7c92\u76f8\u5c0d\u9577\u5ea6(telomere relative length)\u3002\u55ae\u62f7\u8c9d\u57fa\u56e0\u5f15\u7269 (Single-copy gene primers, SCR) \u7279\u7570\u6027\u8b58\u5225\u548c\u64f4\u589e\u4eba\u985e\u67d3\u8272\u9ad4\u4e0a(\u00a0humanchromosome)\u7684 78bp \u9577\u5340\u57df\u3002\u8a66\u5291\u76d2\u4e2d\u7684\u5f15\u7269\u7d44\u5df2\u901a\u904e\u6aa2\u6e2c\uff0c\u78ba\u4fdd\uff1a\uff081\uff09\u9ad8\u6548\u53ef\u9760\u7684\u5b9a\u91cf\uff1b (2)\u975e\u975e\u7279\u7570\u6027\u64f4\u589e\u3002\u6bcf\u7d44\u5f15\u7269\u5747\u7d93\u904e\u64f4\u589e\u66f2\u7dda\u6548\u7387\u9a57\u8b49(\u00a0amplification curve efficiency)\uff08E&gt;98%\uff0cR 2&gt;0.99)\uff0c\u7194\u89e3\u66f2\u7dda\u5206\u6790\u548c\u51dd\u81a0\u96fb\u6cf3\u9a57\u8b49\u3002<\/p>\n<p><strong><span style=\"color: #800000;\">\u7522\u54c1\u63cf\u8ff0: <\/span><\/strong><\/p>\n<p>1.Quickly get results, saving up to 50% of the time<br \/>\n2.Optimized ready-to-use master mix for rapid PCR reactions<br \/>\n3.Accurate detection of various starting amounts of templates, stable amplification, quantitative results with high repeatability<br \/>\n4.Balanced K+ and NH4+ ion ratios, as well as stand-alone ROX Reference Dye packaging for all real-time PCR instruments<\/p>\n<p>EnTurbo\u2122 SYBR Green PCR SuperMix \u5373\u7528\u578b\u9810\u6df7\u6db2\uff0c\u53ef\u4ee5\u5728\u5ee3\u6cdb\u7684\u7bc4\u570d\u5167\u9032\u884c\u7279\u7570\u6027\u548c\u9748\u654f\u7684\u6aa2\u6e2c\u3002\u9810\u6df7\u6db2\u4e2d\u7684 SYBR Green I \u67d3\u6599\u53ef\u4ee5\u5206\u6790\u591a\u7a2e\u76ee\u6a19\u6838\u9178\uff0c\u800c\u7121\u9700\u5408\u6210\u5e8f\u5217\u7279\u7570\u6027\u63a2\u91dd\u3002Antibody method hot-start Taq enzyme\u80fd\u6709\u6548\u6291\u5236primer \u975e\u7279\u7570\u6027nnealing\u3002\u540c\u6642\u512a\u5316\u4e86PCR\u914d\u65b9\uff0c\u9069\u7528\u65bc\u4f4e\u6fc3\u5ea6\u6a21\u677f\u7684\u64f4\u589e\uff0c\u4f7f\u5b9a\u91cfPCR\u80fd\u5920\u5728\u8f03\u5bec\u7684\u5b9a\u91cf\u7bc4\u570d\u5167\u7372\u5f97\u826f\u597d\u7684\u6a19\u6e96\u66f2\u7dda\u3002\u901a\u904e\u7aef\u7c92\u57fa\u56e0\uff08Tel\uff09\u62f7\u8c9d\u6578\u8207\u55ae\u62f7\u8c9d\u57fa\u56e0\uff08SCR\uff09\u62f7\u8c9d\u6578\u7684\u6bd4\u503c\uff08T\/S\uff09\u4f86\u78ba\u5b9a\u6a23\u54c1\u4e2d\u67d3\u8272\u9ad4\u7aef\u7c92\u7684\u5e73\u5747\u9577\u5ea6\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/04\/%E4%BA%BA%E9%A1%9E%E7%AB%AF%E7%B2%92%E9%95%B7%E5%BA%A6%E5%AE%9A%E9%87%8F-qPCR-%E6%AA%A2%E6%B8%AC%E8%A9%A6%E5%8A%91%E7%9B%92-ELK-%E8%B2%A8%E8%99%9FEQ022.jpg\" alt=\"\" width=\"567\" height=\"304\" \/><\/p>\n<p><strong><span style=\"color: #800000;\">Reaction System<\/span><\/strong><\/p>\n<p>A reaction system as described below was established. To perform multiple reactions, prepareapremixofthe common components, add a suitable volume to each tube or well, and then add a special reactioncomponent (eg, template).<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/04\/2.-%E4%BA%BA%E9%A1%9E%E7%AB%AF%E7%B2%92%E9%95%B7%E5%BA%A6%E5%AE%9A%E9%87%8F-qPCR-%E6%AA%A2%E6%B8%AC%E8%A9%A6%E5%8A%91%E7%9B%92-ELK-%E8%B2%A8%E8%99%9FEQ022.jpg\" alt=\"\" width=\"562\" height=\"206\" \/><\/p>\n<p>1. It is recommended to use a 20\u03bcL system to ensure the validity and repeatability of the amplificationofthe gene of interest.<\/p>\n<p>2. Cover or seal the reaction tube\/PCR plate and mix gently. It can be centrifuged slightly toensurethatallcomponents are at the bottom of the tube.<\/p>\n<p>3. Place the reaction system in a real-time PCR instrument, collect data and analyze the results. Setupyour PCR instrument as shown in the table below. Optimum temperature The incubation time can be determined by the specific situation.<\/p>\n<p><a href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/04\/\u4eba\u985e\u7aef\u7c92\u9577\u5ea6\u5b9a\u91cf-qPCR-\u6aa2\u6e2c\u8a66\u5291\u76d2ELK-\u8ca8\u865fEQ022.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/01\/%E7%B6%B2%E7%AB%99%E8%A6%8F%E6%A0%BC%E5%B0%8F%E5%9C%96_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-11-300x97.jpg\" alt=\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df 1_\u5de5\u4f5c\u5340\u57df 1\" width=\"149\" height=\"48\" \/><\/a><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/04\/3-%E4%BA%BA%E9%A1%9E%E7%AB%AF%E7%B2%92%E9%95%B7%E5%BA%A6%E5%AE%9A%E9%87%8F-qPCR-%E6%AA%A2%E6%B8%AC%E8%A9%A6%E5%8A%91%E7%9B%92-ELK-%E8%B2%A8%E8%99%9FEQ022.jpg\" alt=\"\" width=\"545\" height=\"200\" \/><\/p>\n<p><strong><span style=\"color: #993366;\">Three-Step amplification procedure: <\/span><\/strong><\/p>\n<p>Note: The main factors that determine the optimal annealing temperature are primer lengthandprimerbase composition. According to the characteristics of the kit&#8217;s telomere and SCR primer set, werecommend setting the annealing temperature to 55\u00b0C.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/04\/Melt-Curve-PlotTel.jpg\" alt=\"\" width=\"622\" height=\"609\" \/><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/04\/Melt-Curve-Plot36B41.jpg\" alt=\"\" width=\"625\" height=\"649\" \/><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/01\/%E7%B6%B2%E7%AB%99%E8%A6%8F%E6%A0%BC%E5%B0%8F%E5%9C%96-%E5%93%81%E7%89%8C%E4%BB%8B%E7%B4%B9_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1-300x120.jpg\" alt=\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716 \u54c1\u724c\u4ecb\u7d39_\u5de5\u4f5c\u5340\u57df 1\" width=\"146\" height=\"60\" \/><\/p>\n<p><span style=\"font-size: 12pt;\"><span style=\"color: #008000;\"><strong>ELK<\/strong><strong>\u539f\u5ee0\u7db2\u5740<\/strong><\/span><strong><span style=\"color: #008000;\">\u00a0 &gt;&gt;<\/span>\u00a0<span style=\"color: #ff9900;\"><a style=\"color: #ff9900;\" href=\"http:\/\/www.elkbiotech.com\/\">http:\/\/www.elkbiotech.com\/<\/a>\u00a0<\/span><\/strong><\/span><\/p>\n<p>ELK Biotechnology\u516c\u53f8\u662f\u4e00\u5bb6\u5c08\u6ce8\u65bc\u751f\u547d\u79d1\u5b78\u3001\u514d\u75ab\u7814\u7a76\u7684\u9ad8\u79d1\u6280\u751f\u7269\u4f01\u696d\uff0c\u4f4d\u65bcBiolake, Donghu New &amp; High Technology Development Zone, Wuhan city.ELK Biotechnology\u662f\u4e00\u5bb6\u5c08\u6ce8\u65bc\u514d\u75ab\u7522\u54c1\u548c\u5206\u5b50\u751f\u7269\u5b78\u7522\u54c1\u7814\u767c\u548c\u751f\u7522\u7684\u9ad8\u79d1\u6280\u751f\u7269\u516c\u53f8\u3002\u7522\u54c1\u6db5\u84cbELISA\u8a66\u5291\u76d2\u3001\u6297\u9ad4\u3001\u57fa\u56e0\u7d44\u63d0\u53d6\u8a66\u5291\u3001\u5206\u5b50\u751f\u7269\u5b78\u8a66\u5291\u3002\u6d89\u53ca\u514d\u75ab\u5316\u5b78\u3001\u816b\u7624\u7814\u7a76\u3001\u795e\u7d93\u751f\u7269\u5b78\u3001\u7d30\u80de\u9031\u671f\u3001\u4fe1\u865f\u8f49\u5c0e\u7b49\u9818\u57df\u3002\u6211\u5011\u7684\u7522\u54c1\u6709\u56b4\u683c\u7684\u7814\u767c\u548c\u8cea\u91cf\u6aa2\u6e2c\u6d41\u7a0b\u3002\u6bcf\u500b\u7522\u54c1\u90fd\u6709\u5b8c\u6574\u7684\u7814\u767c\u3001\u751f\u7522\u548c\u8cea\u6aa2\u8a18\u9304\u3002\u7d93\u9a57\u8c50\u5bcc\u7684\u7814\u767c\u4eba\u54e1\uff0c\u5b8c\u5584\u7684\u8cea\u6aa2\u652f\u6301\u90e8\u9580\uff0c\u56b4\u683c\u7684\u6aa2\u6e2c\u6d41\u7a0b\uff0c\u70ba\u5ba2\u6236\u63d0\u4f9b\u8cea\u91cf\u53ef\u9760\u7684\u79d1\u7814\u7522\u54c1\u3002<\/p>\n<p><span data-cke-copybin-start=\"1\">\u200b<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u4eba\u985e\u7aef\u7c92\u9577\u5ea6\u5b9a\u91cf qPCR \u6aa2\u6e2c\u8a66\u5291\u76d2 | ELK \u8ca8\u865fEQ022 Human Telomere Length [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":41364,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5524],"tags":[],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/41358"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=41358"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/41358\/revisions"}],"predecessor-version":[{"id":53210,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/41358\/revisions\/53210"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/41364"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=41358"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=41358"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=41358"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}