{"id":41202,"date":"2023-04-18T17:21:06","date_gmt":"2023-04-18T09:21:06","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=41202"},"modified":"2023-04-19T17:36:06","modified_gmt":"2023-04-19T09:36:06","slug":"y-27632-dihydrochloride-rho-%e6%bf%80%e9%85%b6%e6%8a%91%e5%88%b6%e5%8a%91-abcam-cas129830-38-2%e8%b2%a8%e8%99%9fab120129","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=41202","title":{"rendered":"Y-27632 dihydrochloride (Rho \u6fc0\u9176\u6291\u5236\u5291) | ABCAM CAS#129830-38-2\u8ca8\u865fab120129"},"content":{"rendered":"

Y-27632 dihydrochloride (Rho \u6fc0\u9176\u6291\u5236\u5291) | ABCAM CAS#129830-38-2\u8ca8\u865fab120129<\/a><\/span><\/strong><\/p>\n

Y-27632 dihydrochloride, Rho kinase inhibitor Rho \u6fc0\u9176\u6291\u5236\u5291<\/p>\n

Y-27632\u662f\u4e00\u7a2e\u5177\u6709\u5ee3\u6cdb\u751f\u7269\u5b78\u6548\u61c9\u7684\u7814\u7a76\u5de5\u5177\uff0c\u4e3b\u8981\u7528\u65bc\u5e79\u7d30\u80de\u7814\u7a76\u9818\u57df\u7684\u7dad\u6301(\u00a0maintenance)\u548c\u5206\u5316(differentiation)\u3002\u5e38\u7528\u4f5c3D\u751f\u9577\u77e9\u9663\u7d44\u4ef6(growth matrix component)\uff1b\u4e5f\u662f\u8166\u985e\u5668\u5b98\u5206\u5316\u57f9\u990a\u57fa(brain organoid differentiation media)\u7684\u7d44\u6210\u90e8\u5206\u3002<\/p>\n

\u7522\u54c1\u63cf\u8ff0<\/strong>:\u00a0<\/strong><\/span><\/p>\n

Y-27632\u7814\u7a76\u7dad\u6301 iPSC \u7684\u7d44\u7e54\u57f9\u990a\uff1b 10 \u03bcM Y-27632 24 \u5c0f\u6642\uff1b\u8499\u7279\u83f2\u5967\u91cc\u7b49\u4eba (2018)<\/p>\n

\u5c07\u4e73\u817a\u764c\u7d30\u80de\u91cd\u7de8\u7a0b\u70ba\u8a98\u5c0e\u6027\u7956\u7d30\u80de\u6a23\u7d30\u80de (iPL)\uff1b 2\u27e9\u03bcM Y27632 ;\u8881\u7b49\u4eba (2018)
\n\u5206\u5316\u2014\u2014Y-27632 \u4f7f\u4eba\u985e\u80da\u80ce\u5e79\u7d30\u80de\u80fd\u5920\u5b58\u6d3b\u4e26\u5206\u5316\u70ba\u76ae\u8cea\u548c\u57fa\u5e95\u7aef\u8166\u7956\u7d30\u80de\uff1b\u6e21\u908a\u7b49\u4eba (2007)
\n*\u4e5f\u63d0\u4f9b\u7c21\u55ae\u7684\u5132\u5099\u6eb6\u6db2 (ab144494) – \u6dfb\u52a0 1 \u6beb\u5347\u6c34\u4ee5\u7372\u5f97\u6e96\u78ba\u7684\u5373\u7528\u578b\u6fc3\u5ea6\u3002<\/p>\n

Also available in simple stock solutions (ab144494<\/a>) – add 1 ml of water to get an exact, ready-to-use concentration.<\/p>\n

\"\"<\/p>\n

\u529f\u80fd\u7814\u7a76 – Y-27632 \u4e8c\u9e7d\u9178\u9e7d\uff0cRho \u6fc0\u9176\u6291\u5236\u5291 (ab120129)<\/strong><\/span><\/div>\n
\n

\u901a\u904e ICC\/IF\uff0cab46154\u5c0d\u7d93 Y-27632 \u4e8c\u9e7d\u9178\u9e7d (ab120129) \u8655\u7406\u7684 HeLa \u7d30\u80de\u4e2d\u7684 VEGF \u9032\u884c\u67d3\u8272\u3002<\/a>\u5982\u6587\u737b\u6240\u8ff0\uff0cVEGF \u8868\u9054\u7684\u6e1b\u5c11\u8207 Y-27632 \u4e8c\u9e7d\u9178\u9e7d\u6fc3\u5ea6\u7684\u589e\u52a0\u76f8\u95dc\u3002
\n\u5c07\u7d30\u80de\u5728 DMSO \u4e2d\u542b\u6709\u4e0d\u540c\u6fc3\u5ea6\u7684 ab120129\uff08Y-27632 \u4e8c\u9e7d\u9178\u9e7d\uff09\u7684\u57f9\u990a\u57fa\u4e2d\u5728 37\u00b0C \u4e0b\u5b75\u80b2 1 \u5c0f\u6642\uff0c\u5728 -20\u00b0C \u4e0b\u7528 100% \u7532\u9187\u56fa\u5b9a 5 \u5206\u9418\uff0c\u4e26\u7528\u542b\u6709 10% \u5c71\u7f8a\u8840\u6e05\u7684 PBS \u5c01\u9589\uff0c0.3 M \u7518\u6c28\u9178\u30011% BSA \u548c 0.1% \u5410\u6eab\u5728\u5ba4\u6eab\u4e0b\u653e\u7f6e 2 \u5c0f\u6642\u3002\u5728\u542b\u6709 1% BSA \u548c 0.1% \u5410\u6eab\u7684 PBS \u4e2d\uff0c\u5728 4\u00b0C \u4e0b\u7528ab46154<\/a>\u00a0(5 \u00b5g\/ml) \u5c0d\u8655\u7406\u904e\u7684\u7d30\u80de\u9032\u884c\u67d3\u8272\u904e\u591c\u3002DyLight 488 \u5c71\u7f8a\u6297\u5154\u591a\u514b\u9686\u6297\u9ad4 (\u00a0ab96899<\/a>) 1\/250 \u7a00\u91cb\u7528\u4f5c\u4e8c\u6297\u3002\u7d30\u80de\u6838\u7528 DAPI \u8907\u67d3\u4e26\u4ee5\u85cd\u8272\u986f\u793a\u3002<\/p>\n

\"\"<\/p>\n

\u529f\u80fd\u7814\u7a76 – Y-27632 \u4e8c\u9e7d\u9178\u9e7d\uff0cRho \u6fc0\u9176\u6291\u5236\u5291 (ab120129)<\/strong><\/span><\/div>\n
\n

A: \u5728\u4e0d\u540c\u6fc3\u5ea6\u7684 ROCK \u6291\u88fd\u5291 ab120129 \u5b58\u5728\u7684\u60c5\u6cc1\u4e0b\uff0c\u8840\u6e05\u98e2\u9913\u7684\u4eba TM \u7d30\u80de\u5728\u5857\u6709\u91cd\u7d44\u4eba Gal8 \u7684\u8154\u5ba4\u8f09\u73bb\u7247\u4e0a\u5b75\u80b2\u30022 \u5c0f\u6642\u5f8c\uff0c\u7528\u7f85\u4e39\u660e\u6a19\u8a18\u7684\u9b3c\u7b46\u74b0\u80bd\u5c0d\u7d30\u80de\u9032\u884c\u67d3\u8272\uff0c\u4f75\u8a08\u7b97\u5177\u6709\u5805\u56fa\u61c9\u529b\u7e96\u7dad\u7684\u7d30\u80de\u3002\u6578\u64da\u8868\u793a\u70ba\u5e73\u5747\u503c\u00b1SEM\u3002B\uff1a\u7528 20 \u00b5M 120129 (Y27632) \u8655\u7406\u7d30\u80de\uff0c\u7528\u7f85\u4e39\u660e\u6a19\u8a18\u7684\u9b3c\u7b46\u74b0\u80bd\u67d3\u8272\uff0c\u4e26\u62cd\u651d\u96a8\u6a5f\u8996\u91ce\u3002\u8acb\u6ce8\u610f\uff0c\u7528 ab120129 \u8655\u7406\u7684\u7d30\u80de\u4e0d\u6703\u64f4\u6563\u4e26\u5448\u73fe\u6a39\u7a81\u72c0\u7d50\u69cb\u3002<\/p>\n

\u5716\u7247\u4f86\u6e90\uff1aShiri D \u7b49\u4eba\u3002\u516c\u5171\u79d1\u5b78\u5716\u66f8\u9928\u4e00\u865f\u30022012;\u00a07(7)\uff1ae41958\u3002\u00a0doi: 10.1371\/journal.pone.0044400<\/span><\/p>\n<\/div>\n<\/div>\n

\u7522\u54c1\u7279\u9ede<\/strong>\uff1a<\/strong><\/span><\/p>\n

\u9078\u64c7\u6027 Rho \u6fc0\u9176\u6291\u5236\u5291\u30021 \u6beb\u5347\u6c34\u6eb6\u6027\u5305\u88dd\u3002<\/p>\n

CAS \u7de8\u865f\uff1a129830-38-2<\/p>\n

\u7d14\u5ea6\uff1a> 99%<\/p>\n

\u53ef\u6eb6\u65bc 1 ml \u6c34\u4e2d\u4ee5\u63d0\u4f9b\u6307\u5b9a\u7684 mM\/ml \u6fc3\u5ea6<\/p>\n

\u5f62\u5f0f\/\u72c0\u614b\uff1a\u56fa\u9ad4<\/p>\n

\u8cc7\u6599\u4f86\u6e90\uff1a\u5408\u6210<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
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\u00a0K688-100<\/td>\n\u00a0PicoProbe\u2122 Glucose Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
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 <\/p>\n","protected":false},"excerpt":{"rendered":"

Y-27632 dihydrochloride (Rho \u6fc0\u9176\u6291\u5236\u5291) | ABCAM CAS#129830- […]<\/p>\n","protected":false},"author":3,"featured_media":37625,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261],"tags":[5565],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/41202"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=41202"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/41202\/revisions"}],"predecessor-version":[{"id":41207,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/41202\/revisions\/41207"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/37625"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=41202"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=41202"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=41202"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}