{"id":41180,"date":"2023-04-18T10:37:47","date_gmt":"2023-04-18T02:37:47","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=41180"},"modified":"2025-09-03T19:19:32","modified_gmt":"2025-09-03T11:19:32","slug":"g418-sulfate-%e9%81%b8%e6%93%87%e6%96%b0%e9%bb%b4%e7%b4%a0%e6%8a%97%e6%80%a7-neomycin-resistance-nr-%e8%b2%a8%e8%99%9fhy-17561","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=41180","title":{"rendered":"G418 sulfate | CAS#108321-42-2\u9078\u64c7\u65b0\u9ef4\u7d20\u6297\u6027 (neomycin resistance, NR) \u8ca8\u865fHY-17561"},"content":{"rendered":"

G418 sulfate | CAS#108321-42-2\u9078\u64c7\u65b0\u9ef4\u7d20\u6297\u6027 (neomycin resistance, NR)\u00a0 \u8ca8\u865fHY-17561\u00a0<\/a><\/span><\/p>\n

G-418 disulfate (Synonyms: Geneticin sulfate; Antibiotic G-418 sulfate)<\/p>\n

\"\"<\/p>\n

G418 \u4e5f\u7a31\u70ba G-418\u3001G418 sulfate \u6216 Geneticin\uff0cG418 sulfate \u662f\u4e00\u7a2e\u6c28\u57fa\u7cd6\u82f7\u985e\u65b0\u9ef4\u7d20\u985e\u4f3c\u7269(aminoglycoside neomycin analogue)\uff0c\u53ef\u5e72\u64fe\u86cb\u767d\u8cea\u5408\u6210(protein synthesis )\uff0c\u5df2\u5ee3\u6cdb\u7528\u65bc\u9078\u64c7\u5177\u6709\u65b0\u9ef4\u7d20\u6297\u6027 (neomycin resistance, NR) \u7684\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u682a(mammalian cell lines )\u3002\u65b0\u9ef4\u7d20\u6297\u6027 (neomycin resistance, neo) \u57fa\u56e0\u5728\u771f\u6838\u8f09\u9ad4\u4e2d\u7d93\u5e38\u7528\u4f5c\u986f\u6027\u53ef\u9078\u64c7\u57fa\u56e0\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n
Description<\/th>\n\n
IC50<\/sub>\u00a0& Target<\/th>\nAminoglycoside<\/td>\n<\/tr>\n
In Vitro<\/th>\n\n
\n

G418 sulfate, an aminoglycoside neomycin analogue, interferes with protein synthesis and has been used extensively for selection of mammalian cell lines that possess neomycin resistance (NR). The neomycin resistance (neo) gene is frequently used in eukaryotic vectors as a dominant selectable gene. G418 can be covalently bound to asialoorosomucoid (AsOR) to form a conjugate for hepatocyte-specific targeting and toxicity[2]<\/a><\/sup>.
\nThe human GD3 synthase cDNA was transfected into MDA-MB231 cells, and G-418 bulk selection was used to select cells stably overexpressing the GD3 synthase[2]<\/a><\/sup>. An aminoglycoside antibiotic, G418, has been shown to be an inhibitor of many pro- and eukaryotes at concentrations from 1-300 microgram\/ml[4]<\/a><\/sup>.<\/p>\n

MCE has not independently confirmed the accuracy of these methods. They are for reference only.<\/b><\/p>\n<\/div>\n<\/td>\n<\/tr>\n

Molecular Weight<\/th>\n\n
\n

692.71<\/p>\n<\/div>\n<\/td>\n<\/tr>\n

Appearance<\/th>\n\n
\n

Solid<\/p>\n<\/div>\n<\/td>\n<\/tr>\n

Formula<\/th>\n\n
\n

C20<\/sub>H44<\/sub>N4<\/sub>O18<\/sub>S2<\/sub><\/p>\n<\/div>\n<\/td>\n<\/tr>\n

CAS No.<\/th>\n\n
\n

108321-42-2<\/a><\/p>\n<\/div>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n