{"id":41121,"date":"2023-04-17T12:50:16","date_gmt":"2023-04-17T04:50:16","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=41121"},"modified":"2024-05-03T10:01:35","modified_gmt":"2024-05-03T02:01:35","slug":"%e5%bd%97%e6%98%9f%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92%ef%bc%883-well%ef%bc%89-cometassay-kit%e8%b2%a8%e8%99%9fkta3040-75%e5%80%8b%e5%8f%8d%e6%87%893-well","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=41121","title":{"rendered":"\u5f57\u661f\u6aa2\u6e2c\u8a66\u5291\u76d2\uff083 well\uff09| CometAssay Kit\u8ca8\u865fKTA3040 75\u500b\u53cd\u61c9(3-well)"},"content":{"rendered":"

\u5f57\u661f\u6aa2\u6e2c\u8a66\u5291\u76d2\uff083 well\uff09| CometAssay Kit\u8ca8\u865fKTA3040<\/a>\u00a0\u00a075\u500b\u53cd\u61c9(3-well)\u00a0<\/strong><\/span><\/p>\n

Comet Assay Kit (3-Well Slides)<\/span><\/p>\n

CometAssay Kit\u5305\u542b25\u7247\u7279\u5225\u8655\u7406\u7684CometSlide\uff083-well\uff09\u548c\u8db3\u5920\u7684\u8a66\u5291\uff0810xLysis Solution\u3001LMAgarose\u3001\u00a040 mM Tris-HCl (pH 7.5)\u3001\u00a0Propidium Iodide (PI) (50\u00d7)\u548cEDTA (500 mM)\uff09\u7528\u65bc75\u500b\u6e2c\u8a66\u6a23\u54c1\u3002\u63d0\u4f9b\u7368\u6709\u7684\u5f57\u661f\u8f09\u73bb\u7247(CometSlide)\uff0c\u8207\u591a\u5c64\u74ca\u8102\u7cd6\u76f8\u6bd4\uff0c\u6a23\u54c1\u4e0d\u6613\u812b\u843d\u3002\u7121\u9700\u4f7f\u7528\u4e0d\u540c\u7194\u9ede\u74ca\u8102\u7cd6\u7e41\u7463\u7684\u81a0\u6c34\uff0c\u5373\u53ef\u5feb\u901f\u9748\u654f\u5730\u6aa2\u6e2c\u7d30\u80deDNA\u640d\u50b7\uff0c\u64cd\u4f5c\u7c21\u55ae\uff0c\u5167\u9644\u6838\u9178\u67d3\u5291\u3002\u53ef\u6eff\u8db3\u60a8\u5f57\u661f\u5206\u6790\u7684\u6240\u6709\u9700\u8981\u3002<\/p>\n

\u2022 Comet Slides (3-Well)
\n\u2022 10\u00d7 Lysis Solution
\n\u2022 EDTA (500 mM)
\n\u2022 Agarose (Low Melting Point)
\n\u2022 40 mM Tris-HCl (pH 7.5)
\n\u2022 Propidium Iodide (PI) (50\u00d7)<\/p>\n

\"\"<\/p>\n

DNA\u640d\u50b7\u4ecb\u7d39:\u00a0<\/strong><\/span>\u6b63\u5e38\u4ee3\u8b1d\u6d3b\u52d5\u548c\u74b0\u5883\u56e0\u7d20\u5f15\u8d77\u7684DNA\u640d\u50b7\uff0c\u5f15\u767cDNA\u640d\u50b7\u53cd\u61c9\u6fc0\u6d3bDNA\u4fee\u5fa9\u9014\u5f91\u3002 \u5728DNA\u4e0d\u53ef\u9006\u4e4b\u60c5\u6cc1\u4e0b\uff0c\u8a98\u5c0e\u7d30\u80de\u7a0b\u5e8f\u51cb\u4ea1\u3002 \u6b63\u5e38DNA\u7d50\u69cb\u5982DNA\u65b7\u88c2\uff0cDNA adducts \u6216\u4ea4\u806f\uff0c\u9700\u8981DNA\u904e\u7a0b\u4f86\u4fee\u5fa9\u3002 DNA\u4fee\u5fa9\u9700\u8981100\u591a\u500b\u57fa\u56e0\u3002<\/p>\n

\u5f57\u661f\u5206\u6790\u7684\u6aa2\u6e2c\u539f\u7406:<\/span>\u00a0<\/strong>\u662f\u57fa\u65bc\u8b8a\u6027\u7684\u539f\u7406\uff0c\u5f85\u6e2c\u7d30\u80de\u88ab\u88c2\u89e3\u8655\u7406\u4ee5\u4f7fDNA\u89e3\u65cb\u548c\u8b8a\u6027\uff0c\u4e4b\u5f8c\u7d30\u80de\u6838\u9032\u884c\u96fb\u6cf3\uff0c\u4f7f\u5f97\u7d30\u80de\u5728\u5f57\u661f\u73bb\u7247\u4e0a\u5e73\u92ea\u7684\u4f4e\u7194\u9ede\u74ca\u8102\u7cd6\u4e0a\u79fb\u52d5\uff0c\u4e4b\u5f8c\u4f7f\u7528\u8207DNA\u76f8\u4e92\u4f5c\u7528\u7684\u87a2\u5149\u67d3\u6599\u6216\u9280\u67d3\u8a66\u5291\u9032\u884c \u67d3\u8272\u4f7f\u7d50\u679c\u53ef\u8996\u3002\u5f62\u6210\u7684DNA\u5f57\u661f\u5c3e\u5f62\u72c0\u548c\u9077\u79fb\u6a21\u5f0f\uff0c\u5305\u62ec\u9e7c\u6027\u5f57\u661f\u5c3e\u9577\u5ea6\uff0c\u5c3e\u90e8DNA\u767e\u5206\u6bd4\u4ee5\u53ca\u5c3e\u77e9\u7684\u8a08\u7b97\u7b49\u8cc7\u6599\u53ef\u4f7f\u7528\u5716\u50cf\u5206\u6790\u8edf\u9ad4\u9032\u884c\uff0c\u6700\u7d42\u7528\u65bc\u8a55\u4f30\u7d30\u80de\u4e2dDNA\u7684\u640d\u50b7\u3002<\/p>\n

\u5f57\u661f\u5206\u6790<\/strong>\u4e4b\u61c9\u7528<\/strong><\/span>\u00a0\u5f57\u661f\u5206\u6790\u53c8\u7a31\u55ae\u7d30\u80de\u51dd\u81a0\u96fb\u6cf3\u5206\u6790\uff0c\u70ba\u6aa2\u6e2c\u7d30\u80de\u5167DNA\u640d\u50b7\u63d0\u4f9b\u7c21\u4fbf\u6709\u6548\u7684\u65b9\u6cd5\u3002\u81ea\u6210\u529f\u5730\u7528\u65bc\u6aa2\u6e2cDNA\u55ae\u93c8\u65b7\u88c2\u4ee5\u4f86\uff0c\u7d93\u4e0d\u65b7\u5730\u6539\u9032\uff0c\u5df2\u6210\u70ba\u4e00\u7a2e\u5feb \u901f\u3001\u9748\u654f\u548c\u7c21\u4fbf\u5730\u6aa2\u6e2cDNA\u640d\u50b7\u5730\u65b9\u6cd5\uff0c\u5ee3\u6cdb\u5730\u61c9\u7528\u65bcDNA\u653e\u5c04\u640d\u50b7\u3001DNA\u526a\u5207\u640d\u50b7\u3001DNA\u4ea4\u806f\u7684\u6aa2\u6e2c\u3001\u85e5\u7269\u7684\u907a\u50b3\u6bd2\u6027\u8a55\u50f9\u548c\u7d30\u80de\u51cb\u4ea1\u9452\u5b9a\u7b49\u5de5\u4f5c\u4e2d\u3002\u666e\u901a\u7684\u5f57\u661f\u5206\u6790\u53ca\u87a2\u5149\u67d3\u8272\u7522\u751f\u7684\u8cc7\u6599\u7d50\u679c\u9700\u8981\u87a2\u5149\u986f\u5fae\u93e1\u9032\u884c\u89c0\u5bdf\uff0c\u4e26\u4e14\u4e0d\u6613\u4fdd\u5b58\uff0c\u5982\u679c\u80fd\u7372\u5f97\u6c38\u4e45\u7684\u5f57\u661f\u5206\u6790\u5716\u50cf\u7d50\u679c\uff0c\u4e26\u53ef\u5728\u666e\u901a\u986f\u5fae\u93e1\u4e0b\u6aa2\u6e2c\u5247\u53ef\u4ee5\u63d0\u9ad8\u5f57\u661f\u5206\u6790\u7684\u4fbf\u6377\u6027\u3002<\/p>\n

\"\"<\/p>\n

Fig.The Abbkine Comet Assay Kit was used to detect DNA damage in Jurkat normal cells and DNA damage induced positive control group. And it\u2019s compared with other brand A. The fluorescence brightness and clarity detected by Abbkine Comet Assay Kit are higher than the other brand.<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong><\/span><\/p>\n

Comet \u6aa2\u6e2c\u8a66\u5291\u76d2\uff083 \u5b54\u8f09\u73bb\u7247\uff09\u662f\u4e00\u7a2e\u55ae\u7d30\u80de\u51dd\u81a0\u96fb\u6cf3\u6aa2\u6e2c (single cell gel electrophoresis assay, SCGE)\uff0c\u53ef\u5feb\u901f\u9748\u654f\u5730\u6aa2\u6e2c\u7d30\u80de\u4e2d\u7684 DNA \u640d\u50b7(DNA damage )\u3002\u5176\u539f\u7406\u662f\u5668\u5b98\u6216\u7d44\u7e54\u906d\u53d7\uff0c\u5982\u8f3b\u5c04(\u00a0radiation)\u3001\u91cd\u91d1\u5c6c\u7b49(heavy metals)\u640d\u50b7\u5f8c\uff0c\u7d30\u80de\u5167\u7684\u55ae\u934a\u6216\u96d9\u93c8DNA(double strand DNA)\u65b7\u88c2\uff0c\u5c07\u7d30\u80de\u8207\u7194\u5316\u7684\u74ca\u8102\u7cd6(melted agarose)\u6df7\u5408\u5f8c\u52a0\u5165\u5f57\u661f\u8f09\u73bb\u7247(CometSlide)\u4e0a\uff0c\u7136\u5f8c\u7d30\u80de\u819c(\u00a0cell membrane )\u88ab\u88c2\u89e3\u6db2(\u00a0lysis solution)\u7834\u58de\uff0cDNA\u88ab\u9e7c\u6027\u6eb6\u6db2(alkaline solution)\u89e3\u958b\u3002\u6700\u5f8c\u5c07\u6a23\u54c1\u9032\u884c\u96fb\u6cf3(electrophoresed)\uff0c\u5728\u96fb\u5834\u4f5c\u7528(electric field)\u4e0b\uff0c\u6b63\u5e38\u7d30\u80de\u7684\u5927\u5206\u5b50DNA(macromolecular DNA)\u9077\u79fb\u8ddd\u96e2\u8f03\u77ed\uff0c\u5b8c\u6574\u7684DNA\u7559\u5728\u7d30\u80de\u6838(nucleus)\u7bc4\u570d\u5167\uff0cDNA\u788e\u7247\u6e38\u96e2\u51fa\u7d30\u80de\u6838(nucleus)\uff0cPI\u67d3\u8272\u67d3\u6599\uff0c\u5728\u87a2\u5149\u986f\u5fae\u93e1\u4e0b\u89c0\u5bdf\uff0c\u5b8c\u6574\u7684DNA\u5f62\u6210\u5713\u5f62\u6216\u5fae\u5e36\u5c3e\u7684\u5716\u6848\uff1b DNA \u788e\u7247\u7247\u6bb5\u5f62\u6210\u5f57\u661f\u72c0\u5716\u6848(comet-like pattern)\u3002<\/p>\n

    \n
  1. \u9069\u7528\u65bc\u55ae\u934a\u6216\u96d9\u93c8DNA\u65b7\u88c2\u7684\u5206\u6790<\/li>\n
  2. \u7814\u7a76\u7d30\u80de\u5c0dDNA\u640d\u50b7\u7684\u53cd\u61c9<\/li>\n
  3. \u7be9\u9078DNA\u4fee\u5fa9\u6291\u88fd\u5291<\/li>\n
  4. \u6e2c\u8a66\u5316\u5b78\u54c1\u7684\u6bd2\u6027<\/li>\n
  5. \u74b0\u5883\u8a98\u8b8a\u5291\u7be9\u9078<\/li>\n<\/ol>\n

    \u5f57\u661f\u5206\u6790\u64cd\u4f5c\u6d41\u7a0b\uff1a<\/strong><\/span><\/p>\n

    \"\"<\/p>\n

    \u6ce8\u610f\u4e8b\u9805\uff1a<\/span><\/strong>\u8a66\u5291\u65b0\u9bae\u914d\u88fd\uff1b\u5c0d\u65bc\u542b\u6709\u8840\u7d05\u7d20\u7684\u6a23\u54c1\uff0c\u4f8b\u5982\u8840\u7d30\u80de\u548c\u7d44\u7e54\uff0cDMSO \u53ef\u4ee5\u4ee5 10% \u7684\u7d42\u6fc3\u5ea6\u6dfb\u52a0\u3002\u4f7f\u7528\u524d\u5c07 1\u00d7Lysis Buffer \u5728 4\u2103 \u51b7\u537b\u81f3\u5c11 20 \u5206\u9418\u3002 1\u00d7LysisBuffer\u5728\u5ba4\u6eab\u4e0b\u5448\u6df7\u6fc1\u72c0\uff0c4\u2103\u6642\u6703\u8b8a\u6f84\u6e05\uff0cpH\u503c\u4fdd\u6301\u572810\u5de6\u53f3\u3002<\/p>\n

    Alkaline Solution\u6eb6\u6db2\u914d\u88fd<\/span><\/strong><\/p>\n

    \"\"<\/p>\n

    TBE Electrophoresis Solution\u6eb6\u6db2\u914d\u88fd<\/strong><\/span><\/p>\n

    \"\"<\/p>\n

    Alkaline Electrophoresis Solution (300 mM NaOH, 1 mM EDTA, pH>13)\u6eb6\u6db2\u914d\u88fd<\/span><\/strong><\/p>\n

    \"\"<\/p>\n

    \u5f57\u661f\u5206\u6790\u64cd\u4f5c\u6280\u8853\u8cc7\u6599 (Data Analysis The DNA damage)\u00a0<\/strong><\/span><\/p>\n

    \u6280\u8853\u8cc7\u6599\u3010\u9810\u51b7\u7de9\u885d\u6db2\u3011<\/span><\/strong><\/p>\n

    \u5be6\u9a57\u524d\u6e96\u5099\u6240\u6709\u6eb6\u6db2\u51b7\u537b\u81f34\u2103: 1\u00d7\u88c2\u89e3\u7de9\u885d\u6db2(1\u00d7Lysis Buffer)\u3001\u9e7c\u6027\u6eb6\u6db2(Alkaline Solution)\u53ca\u96fb\u6cf3\u6eb6\u6db2(Electrophoresis Solution)\uff08\u8acb\u53c3\u95b1\u8a66\u5291\u6e96\u5099\uff09\u3002
    \n\u4f7f\u7528\u524d\u5c07\u6240\u6709\u6eb6\u6db2\u51b7\u537b\u81f34\u2103\u3002\u6700\u5f8c\u986f\u5fae\u93e1\u89c0\u5bdf\u4ee5400X\u64f7\u53d6\u5f71\u50cf, \u6700\u4f73\u8996\u91ce\u70ba75\u9846\u7d30\u80de\u3002<\/p>\n

    \u6280\u8853\u8cc7\u6599\u3010\u96fb\u6cf3Buffer\u4e4b\u9078\u64c7- Electrophoresis solution\u3011<\/span><\/strong><\/span><\/p>\n

    \u6839\u64da\u6240\u9700\u7684\u904b\u4f5c\u689d\u4ef6\u548c\u6aa2\u6e2c\u9748\u654f\u5ea6\u9078\u64c7\u5408\u9069\u7684\u96fb\u6cf3\u6eb6\u6db2\u3002 TBE \u66f4\u9069\u5408\u7d30\u80de\u51cb\u4ea1\u5206\u6790(apoptosis )\uff0c\u4e26\u4e14\u53ef\u4ee5\u4f7f\u7528\u5c3e\u9577( tail length)\u800c\u4e0d\u662f\u5c3e\u77e9\u9032(tail moment)\u884c\u6578\u64da\u5206\u6790\u3002 TBE \u96fb\u6cf3(TBE electrophoresisdetects) \u53ef\u5075\u6e2c\u55ae\u934a(single-stranded )\u548c\u96d9\u80a1 DNA \u65b7\u88c2(double-stranded DNA)\uff0c\u4e26\u53ef\u5075\u6e2c\u4e00\u4e9b AP \u4f4d\u9ede(AP sites)\u3002\u9e7c\u6027\u96fb\u6cf3( Alkaline electrophoresisis)\u66f4\u9748\u654f\uff0c\u53ef\u4ee5\u6aa2\u6e2c\u51fa\u66f4\u5c11\u91cf\u7684 DNA \u640d\u50b7(DNA damage)\u3002\u9e7c\u6027\u96fb\u6cf3(Alkaline electrophoresis)\u53ef\u4ee5\u6aa2\u6e2c\u55ae\u934a(single-stranded)\u548c\u96d9\u80a1 DNA \u65b7\u88c2(double-stranded DNA breaks)\u3001\u5927\u591a\u6578 AP \u4f4d\u9ede\u4ee5\u53ca\u9e7c\u4e0d\u7a69\u5b9a DNA \u52a0\u5408\u7269\u3002<\/p>\n

    \u5f57\u661f\u5206\u6790\u8cc7\u6599\u5206\u6790 (Data Analysis The DNA damage)\u00a0<\/strong><\/span><\/p>\n

    \u6578\u64da\u5206\u6790 DNA \u640d\u50b7\u662f\u901a\u904e\u6e2c\u91cf\u7d30\u80de\u6838\u907a\u50b3\u7269\u8cea\uff08\u201c\u5f57\u661f\u982d(comet head)\u201d\uff09\u548c\u7531\u6b64\u7522\u751f\u7684\u201c\u5c3e\u5df4\u201d\u4e4b\u9593\u7684\u4f4d\u79fb\u4f86\u91cf\u5316\u7684\u3002 Tail Moment \u548c Tail DNA% \u662f\u5206\u6790 Comet \u6aa2\u6e2c\u7d50\u679c\u7684\u5169\u500b\u6700\u5e38\u7528\u53c3\u6578\u3002\u6bcf\u500b\u6a23\u54c1\u81f3\u5c11\u61c9\u5206\u6790 50-100 \u500b\u7d30\u80de\u3002\u8003\u616e\u5230\u907a\u50b3\u7269\u8cea\u7684\u9077\u79fb\u4ee5\u53ca\u5c3e\u90e8 DNA \u7684\u76f8\u5c0d\u91cf\uff0c\u5c3e\u77e9(Tail Moment )\u88ab\u8a8d\u70ba\u662f\u8a98\u5c0e DNA \u640d\u50b7\u7684\u9069\u7576\u6307\u6a19\u3002<\/p>\n

    Data Analysis The DNA damage is quantified by measuring the displacement between the genetic material of the nucleus (‘comet head’) andtheresulting ‘tail’. Tail Moment and Tail DNA% are the two most common parameters to analyze Comet assay results. At least 50-100cells should be analyzed per sample. The Tail Moment has been suggested to be an appropriate index of induced DNAdamageinconsidering both the migration of the genetic material as well as the relative amount of DNA in the tail.<\/p>\n

    Tail DNA%=Tail DNA Intensity\/Cell DNA Intensity\u00d7100<\/p>\n

    Tail Moment can be measured using one of the following methods:<\/p>\n

    (a) Olive Tail Moment=Tail DNA%\u00d7Tail Moment Length (measured from the center of the head to the center of the tail, seeFigure1)<\/p>\n

    (b) Extent Tail Moment=Tail DNA%\u00d7Tail Length( see Figure 1) Comet Assay results analysis software is available through Opencomet, CASPlab and Comet Assay IV (Perceptive Instruments),among others.<\/p>\n

    \"\"<\/p>\n

    <\/p>\n

    BRAND\/AGENCY\/\u53f0\u7063\u4ee3\u7406\u5546<\/span>\/\u00a0<\/span>Abbkine\u53f0\u7063\u4ee3\u7406\u5546<\/a><\/span><\/span><\/strong><\/h2>\n

    \"abbkine\u5546\u54c1\u63d2\u5716\"\"abbkine02\"\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0\u00a0\"\u539f\u5ee0\u9023\u7d5001\"<\/a><\/p>\n

    \"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716<\/p>\n

    https:\/\/www.abbkine.com\/<\/p>\n

    Abbkine Scientific Co., Ltd\u7531\u4e00\u7fa4\u5fd7\u540c\u9053\u5408\u7684\u79d1\u5b78\u5bb6\u548c\u884c\u92b7\u5c08\u5bb6\uff0c\u57282012\u5e74\u6210\u7acb\u65bc\u7f8e\u570b\u52a0\u5229\u798f\u5c3c\u4e9e\u5dde\uff0c\u96a8\u8457\u4e9e\u6d32\u5e02\u5834\u7684\u8fc5\u731b\u589e\u9577\uff0cAbbkine\u5c07\u7e3d\u90e8\u642c\u9077\u81f3\u4e2d\u570b\u3002\u7d50\u5408\u4e86Abbkine\u7f8e\u570b\u6280\u8853\u548c\u4e2d\u570b\u88fd\u9020\u5169\u65b9\u9762\u7684\u5de8\u5927\u512a\u52e2\uff0cAbbkine\u81f4\u529b\u65bc\u63d0\u4f9b\u5275\u65b0\u7684\u3001\u9ad8\u54c1\u8cea\u7684\u3001\u8cb7\u5f97\u8d77\u7684\u5be6\u9a57\u8a66\u5291\u76d2\u3001\u86cb\u767d\u8cea\u3001\u6297\u9ad4\u4ee5\u53ca\u5176\u5b83\u7814\u7a76\u5de5\u5177\uff0c\u4ee5\u671f\u6210\u70ba\u751f\u547d\u79d1\u5b78\u7814\u7a76\u767c\u5c55\uff0c\u85e5\u7269\u7814\u767c\u7b49\u9818\u57df\u7684\u95dc\u9375\u63a8\u52d5\u8005\u3002<\/p>\n

    \u767c\u5c55\u6b77\u7a0b<\/span><\/p>\n

    Abbkine\u81f4\u529b\u65bc\u63d0\u4f9b\u5275\u65b0\u7684\u3001\u9ad8\u54c1\u8cea\u7684\u3001\u8cb7\u5f97\u8d77\u7684\u5be6\u9a57\u8a66\u5291\u76d2\u3001\u86cb\u767d\u8cea\u3001\u6297\u9ad4\u4ee5\u53ca\u5176\u5b83\u7814\u7a76\u5de5\u5177\u4ee5\u671f\u6210\u70ba\u751f\u547d\u79d1\u5b78\u7814\u7a76\u767c\u5c55\uff0c\u85e5\u7269\u7814\u767c\u7b49\u9818\u57df\u7684\u95dc\u9375\u63a8\u52d5\u8005\u3002Abbkine\u4ee5\u7279\u8272\u7684\u86cb\u767d\u8cea\u751f\u7522\u8207\u6aa2\u6e2c\u8a66\u5291\u8d77\u5bb6\uff0c\u81f4\u529b\u65bc\u5275\u65b0\u548c\u7814\u767c\u5404\u985e\u6297\u9ad4\u3001\u751f\u5316\u8a66\u5291\u3001\u86cb\u767d\u8cea\u548c\u5be6\u9a57\u8a66\u5291\u76d2\uff0c\u4ee5\u671f\u671b\u70ba\u5168\u7403\u79d1\u7814\u5de5\u4f5c\u8005\u63d0\u4f9b\u9769\u65b0\u7684\u89e3\u6c7a\u65b9\u6848\u3002\u6211\u5011\u5f88\u81ea\u8c6a\u5730\u70ba\u5ba2\u6236\u63d0\u4f9b\u5982\u4e0b\u7279\u8272\u7522\u54c1\u7dda\uff0c\u4e26\u6703\u4e00\u5982\u65e2\u5f80\u5730\u958b\u767c\u65b0\u578b\u7684\u3001\u9ad8\u54c1\u8cea\u7684\u3001\u8cb7\u5f97\u8d77\u7684\u751f\u547d\u79d1\u5b78\u7814\u7a76\u548c\u8a3a\u65b7\u8a66\u5291\u7522\u54c1\u30022017\u5e74\u6aa2\u6e2c\u7d30\u80de\u56e0\u6578\u548c\u6fc0\u7d20\u7684ELISA\u8a66\u5291\u76d2\u61c9\u904b\u800c\u751f: \u6839\u64da\u5e02\u5834\u9700\u6c42\uff0c\u958b\u767cEliKine\u2122\u7cfb\u5217ELISA\u8a66\u5291\u76d2\uff0c\u6db5\u84cb\u5404\u985e\u7d30\u80de\u56e0\u6578\u548c\u6fc0\u7d20\u71b1\u9580\u6307\u6a19\uff0c\u5982\u767d\u4ecb\u7d20\uff08IL\uff09\u3001\u816b\u7624\u58de\u6b7b\u56e0\u6578\uff08TNF\uff09\u3001\u5e72\u64fe\u7d20\uff08IFN\uff09\u7b49\u660e\u661f\u7d1a\u5076\u806f\u6a19\u8a18\u8a66\u5291\u76d2\u5168\u7403\u767c\u552e\u30022018\u5e74\u6191\u85c9\u5148\u9032\u7684\u86cb\u767d\u8cea\u5076\u806f\u6280\u8853\u548c\u56b4\u683c\u7684\u54c1\u8cea\u63a7\u5236\uff0c\u6211\u5011\u63d0\u4f9bLinKine\u2122\u5076\u806f\u6a19\u8a18\u8a66\u5291\u76d2\uff0c\u5728\u6297\u9ad4\u3001\u86cb\u767d\u8cea\u3001\u591a\u80bd\u6216\u8005\u5176\u5b83\u751f\u7269\u5206\u5b50\u4e0a\u53ef\u76f4\u63a5\u6a19\u8a18HRP\uff0cBiotin\uff0cFITC\uff0cCy3\uff0cAbFluor\u2122\u4ee5\u6eff\u8db3\u79d1\u7814\u61c9\u7528\uff0c\u85e5\u7269\u7814\u767c\u4ee5\u53ca\u8a3a\u65b7\u8a66\u5291\u76d2\u958b\u767c\u7684\u9700\u6c42\u30022019\u5e74\u751f\u5316\u3001\u7d30\u80de\u985e\u7522\u54c1\u7dda\u9010\u6b65\u64f4\u5145\u642d\u5efa\u7d30\u80de\u7522\u54c1\u7814\u767c\u5e73\u81fa\uff0c\u7d30\u80de\u72c0\u614b\u6aa2\u6e2c\u3001\u7d30\u80de\u67d3\u8272\u3001\u7d30\u80de\u63d0\u53d6\u3001\u751f\u5316\u8a66\u5291\u76d2\u7522\u54c1\u5f97\u5230\u8d8a\u4f86\u8d8a\u591a\u5168\u7403\u5ba2\u6236\u7684\u8a8d\u53ef\u3002<\/p>\n

    \"\"<\/p>\n

      \n
    1. \u5168\u5957\u8868\u4f4d\u6a19\u7c64\u6297\u9ad4\u548c\u5167\u53c3\u6297\u9ad4\uff0c\u4ee5\u53ca\u76f8\u61c9\u7684\u5b9a\u5236\u5076\u806f\u7522\u54c1<\/li>\n
    2. \u5c0f\u5305\u88dd\u7684\u78f7\u9178\u5316\u548c\u7532\u57fa\u5316\u7279\u7570\u6027\u7684\u6297\u9ad4\u7522\u54c1<\/li>\n
    3. \u6700\u71b1\u9580\u7684\u4e8c\u6297\u7522\u54c1\uff1a\u7528\u65bcIF\u7684IFKine\u7cfb\u5217\u7522\u54c1\uff0c\u4ee5\u53caIP-WB\u7684IPKine\u7cfb\u5217\u7522\u54c1<\/li>\n
    4. \u5168\u5957\u9ad8\u54c1\u8cea\uff0c\u8cb7\u5f97\u8d77\u7684\u86cb\u767d\u8cea\u7d14\u5316\u5de5\u5177\uff08\u586b\u6599\uff0c\u9810\u88dd\u67f1\u548c\u8a66\u5291\u76d2\uff09<\/li>\n
    5. \u66a2\u92b7\u7684\u91cd\u7d44\u86cb\u767d\uff0c\u5305\u542b\u5404\u985e\u7d30\u80de\u56e0\u6578\u548c\u751f\u9577\u56e0\u6578<\/li>\n
    6. \u66f4\u591a\u7d30\u80de\u529f\u80fd\u5206\u6790\u8a66\u5291\u76d2\uff0cELISA\u8a66\u5291\u76d2\uff0c\u4ee5\u53ca\u7d30\u80de\u793a\u8e64\u8a66\u5291<\/li>\n<\/ol>\n

      \u6b64\u5916\uff0c\u6211\u5011\u9084\u5efa\u7acb\u4e86\u6280\u8853\u670d\u52d9\u5e73\u81fa\uff0c\u5305\u62ec\u514d\u75ab\u5b78\u76f8\u95dc\u5be6\u9a57\u670d\u52d9\uff08WB\/IHC\/IF\/IP\uff09\u3001\u86cb\u767d\u5076\u806f\u670d\u52d9\u3001\u86cb\u767d\u8868\u9054\u3001\u751f\u5316\/\u7d30\u80de\u5b78\u5be6\u9a57\u4ee3\u6e2c\u3001Elisa\u5be6\u9a57\u4ee3\u6e2c\u3001\u6297\u9ad4\u88fd\u5099\u7b49\uff0c\u6191\u85c9\u5c08\u696d\u6280\u8853\u3001\u8a2d\u5099\u548c\u670d\u52d9\u614b\u5ea6\uff0c\u70ba\u5ba2\u6236\u63d0\u4f9b\u4e00\u7ad9\u5f0f\u5be6\u9a57\u89e3\u6c7a\u65b9\u6848\u3002Abbkine\u6642\u523b\u628a\u63e1\u5ba2\u6236\u9700\u6c42\u8b8a\u5316\uff0c\u4e26\u7a4d\u6975\u61c9\u5c0d\u3001\u53ca\u6642\u8abf\u6574\uff0c\u4f7f\u5f97Abbkine\u6210\u70ba\u8a31\u591a\u570b\u969b\u9818\u5148\u7684\u7814\u7a76\u6a5f\u69cb\u548c\u81e8\u5e8a\u8a3a\u65b7\u516c\u53f8\u503c\u5f97\u4fe1\u8cf4\u7684\u53ef\u9760\u4f9b\u61c9\u5546\u3002<\/p>\n

      \"\u8840\u6e05\"<\/p>\n

      \u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\uff09\u00a0|\u00a0\u8ca8\u865fKTA3030<\/a>\u00a0<\/span><\/strong><\/p>\n

      Cell Senescence Kit (\u03b2-Galactosidase Assay)<\/span><\/p>\n

      \u7d30\u80de\u8870\u8001(Cellular senescence)\u662f\u6b63\u5e38\u7d30\u80de\u505c\u6b62\u5206\u88c2(cell divide)\u7684\u73fe\u8c61\u3002\u8870\u8001\u7684\u7d30\u80de(senescent cells)\u4e0d\u518d\u80fd\u5920\u8907\u88fd(replicate)\uff0c\u4f46\u5b83\u5011\u4ecd\u4fdd\u6301\u4ee3\u8b1d\u6d3b\u6027(metabolically active)\uff0c\u4f46\u56e0\u963b\u6eef\u65bcG1\u671f\uff0c\u5931\u53bb\u4e86\u5c0d\u6709\u7d72\u5206\u88c2\u539f\u7684\u53cd\u61c9\u80fd\u529b\u548c\u5408\u6210DNA\u7684\u80fd\u529b\uff0c\u4e0d\u80fd\u9032\u5165S\u671f\u3002\u4e26\u4e14\u8207\u8870\u8001\u76f8\u95dc\u7684\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\u6d3b\u6027\u67d3\u8272(senescence-associated beta-galactosidase activity)\u5448\u967d\u6027\uff0c\u9019\u88ab\u8a8d\u70ba\u662f\u7d30\u80de\u8870\u8001\u7684\u751f\u7269\u6a19\u8a18(biomarker)\u3002\u7d30\u80de\u7684\u8870\u8001(Cellular senescence)\u53ef\u4ee5\u901a\u904e\u6fc0\u6d3b\u764c\u57fa\u56e0(oncogenes)\u548c\u7d30\u80de-\u7d30\u80de\u878d\u5408(cell-cell fusion)\u4f86\u5be6\u73fe\uff0cDNA\u5c0d\u6d3b\u6027\u6c27\uff08reactive oxygen species , ROS\uff09\u5347\u9ad8\u7684\u53cd\u61c9\u6703\u5c0e\u81f4DNA\u640d\u50b7(DNA damage)\uff0c\u800c\u4e0d\u662f\u50c5\u50c5\u4f9d\u8cf4\u65bc\u7d30\u80de\u5206\u88c2\u7684\u6578\u76ee\u3002\u5728\u6b63\u5e38\u8870\u8001\u904e\u7a0b(normal aging)\u4e2d\uff0c\u7d44\u7e54\u4e2d\u7684\u8870\u8001\u7d30\u80de\u6578\u91cf\u986f\u8457\u589e\u52a0\u3002<\/p>\n

      \"abbkine\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

      \u7d30\u80de\u662f\u751f\u7269\u7d50\u69cb(biological structure)\u548c\u529f\u80fd(function)\u7684\u57fa\u672c\u55ae\u4f4d\uff0c\u4e5f\u662f\u751f\u7269\u8870\u8001(biological aging)\u7684\u57fa\u672c\u55ae\u4f4d\u3002\u7d30\u80de\u8870\u8001(Cellular senescence)\u5728\u5f62\u614b\u4e0a\u8868\u73fe\u70ba\u7d30\u80de\u7d50\u69cb(cell structure)\u7684\u9000\u5316\uff0c\u4f8b\u5982\u6838\u819c\u51f9\u9677(nuclear membrane depression)\uff0c\u6700\u7d42\u5c0e\u81f4\u6838\u819c\u584c\u9677(nuclear membrane collapse)\uff0c\u67d3\u8272\u8cea\u7d50\u69cb(chromatin structure)\u6539\u8b8a\uff0c\u8d85\u4e8c\u500d\u9ad4(hyperdiploid)\u548c\u7570\u5e38\u591a\u500d\u9ad4\u7d30\u80de(polyploid cells)\u6578\u91cf\u589e\u52a0\u3002\u7d30\u80de\u819c\u7684\u8106\u6027(cell membrane fragility)\u589e\u52a0\u3002\u901a\u900f\u6027(permeability)\u964d\u4f4e\uff0c\u819c\u53d7\u9ad4(membrane receptors)\u7684\u985e\u578b\u548c\u6578\u91cf\u4ee5\u53ca\u5c0d\u914d\u9ad4(ligands)\u7684\u654f\u611f\u6027\u767c\u751f\u8b8a\u5316\uff1b\u8102\u8910\u7d20(lipofuscin)\u5728\u7d30\u80de\u4e2d\u7a4d\u7d2f\uff0c\u8a31\u591a\u7d30\u80de\u5668\u548c\u7d30\u80de\u5167\u7d50\u69cb\u767c\u751f\u8b8a\u6027\u3002\u7d30\u80de\u8870\u8001(Cell senescence)\u5728\u751f\u7406\u4e0a\u8868\u73fe(physiologically manifested)\u70ba\u529f\u80fd\u4e0b\u964d\u548c\u65b0\u9673\u4ee3\u8b1d\u4f4e\u4e0b\uff0c\u4f8b\u5982\u7d30\u80de\u9031\u671f\u505c\u6eef(cell cycle arrest)\uff0c\u7d30\u80de\u8907\u88fd\u80fd\u529b (cell replication ability) \u55aa\u5931\uff0c\u5c0d\u4fc3\u6709\u7d72\u5206\u88c2\u523a\u6fc0(mitogenic stimulation)\u7684\u53cd\u61c9\u6027\u6e1b\u5f31\u4ee5\u53ca\u5c0d\u4fc3\u51cb\u4ea1\u56e0\u5b50(pro-apoptotic factors)\u7684\u53cd\u61c9\u6027\u6539\u8b8a\u3002\u7d30\u80de\u5167\u9176\u6d3b\u6027\u4e2d\u5fc3\u88ab\u6c27\u5316\uff0c\u9176\u6d3b\u6027(Enzyme activity)\u964d\u4f4e\uff0c\u86cb\u767d\u8cea\u5408\u6210(protein synthesis)\u964d\u4f4e\u7b49\u3002\u8870\u8001\u7d30\u80de(senescent cells)\u4e0d\u518d\u8907\u5236\uff0c\u4f46\u5b83\u5011\u4ecd\u4fdd\u6301\u4ee3\u8b1d\u6d3b\u6027(metabolically active)\uff0c\u4e26\u4e14\u8870\u8001\u76f8\u95dc\u7684\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\u6d3b\u6027(beta-galactosidase activity)\u5448\u967d\u6027\uff0c\u9019\u88ab\u8a8d\u70ba\u662f\u7d30\u80de\u8870\u8001\u751f\u7269\u6a19\u8a18(biomarker of cellular senescence)\u3002<\/p>\n

      \"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

      Abbkine\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08Senescence \u03b2-Galactosidase Staining Kit, KTA3030\uff09\u63d0\u4f9b\u4e86\u57fa\u65bc\u8870\u8001\u904e\u7a0b\u4e2dpH\u503c\u70ba6\u7684\u8870\u8001\u76f8\u95dc\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\uff08SA-\u03b2-Gal\uff09\u6d3b\u6027\u4e0a\u8abf\u7684\u8a66\u5291\uff0c\u7528\u65bc\u6aa2\u6e2c\u8870\u8001\u7d30\u80de(senescent cells)\u6216\u7d44\u7e54(tissues)\u3002\u7d30\u80de\u6216\u7d44\u7e54\u7684\u8001\u5316\u53ef\u4ee5\u5728\u666e\u901a\u7684\u5149\u5b78\u986f\u5fae\u93e1\u4e0b\u89c0\u5bdf\u3002 SA-\u03b2-Gal\u50c5\u5b58\u5728\u65bc\u8870\u8001\u7d30\u80de(senescent cells)\u4e2d\uff0c\u5728\u8870\u8001\u524d\uff0c\u975c\u6b62\uff0c\u816b\u7624\u6216\u6c38\u751f\u7d30\u80de\u4e2d\u7121\u6cd5\u6aa2\u6e2c\u5230Senescence \u03b2-Galactosidase Staining Kit\u00a0 provide reagents for detecting the senescent cells or tissues based on the up-regulation of senescence-associated \u03b2-galactosidase (SA-\u03b2-Gal) activity at pH 6 during aging. The aging of cells or tissues can be observed under a common optical microscope. SA-\u03b2-Gal is present only in senescent cells and is undetectable in presenescent, quiescent, tumor or immortal cells<\/p>\n

      \u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/p>\n

      \u7d30\u80de\u589e\u6b96&\u6bd2\u7406&\u8870\u8001 (8)<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
      \u7522\u54c1\u8ca8\u865f<\/span><\/strong><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/strong><\/td>\n\u53cd\u61c9<\/span><\/strong><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/strong><\/td>\n<\/tr>\n
      \u8ca8\u865fKTA1001<\/a><\/span><\/td>\n\u6d3b&\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u8a66\u5291\u76d2<\/a><\/span><\/td>\n100\u500b\u53cd\u61c9<\/span><\/td>\n\n

      \u6d3b\u7d30\u80de\u7da0\u8272\u87a2\u5149\u67d3\u5291Ex\/Em = 488\/530 nm;<\/span><\/p>\n

      \u6b7b\u7d30\u80de\u7d05\u8272\u87a2\u5149\u67d3\u6599(Ex\/Em = 535\/617)<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>KTA3030<\/a><\/span><\/td>\n\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\uff09<\/a><\/span><\/td>\n100\u500b\u53cd\u61c9<\/span><\/td>\n\u5149\u5b78\u986f\u5fae\u93e1<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTA1020<\/a><\/span><\/td>\nCCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/span><\/td>\n1000\u500b\u53cd\u61c9<\/span><\/td>\n\u5438\u6536\u5149450nm<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTA2030<\/a><\/span><\/td>\nEdU Image\u7d30\u80de\u589e\u6b96\u8a66\u5291\u76d2<\/a>\u00a0(BrdU\u66ff\u4ee3\u6cd5)<\/span><\/td>\n100\u500b\u53cd\u61c9<\/span><\/td>\nAbFluor 488 azide (Ex\/Em = 501\/525 nm)<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTB1110<\/a><\/span><\/td>\n\uff08LDH\uff09\u4e73\u9178\u812b\u6c2b\u9176\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9\/<\/span><\/td>\n\n

      \u03bbmax= 450 nm<\/span><\/p>\n

      1- 20 U\/mL of LDH<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>\u00a0KTA2020<\/a><\/span><\/td>\n\u7d30\u80de\u9031\u671f\u67d3\u8272\u6aa2\u6e2c<\/a><\/span><\/td>\n50\u500b\u53cd\u61c9\/100\u500b\u53cd\u61c9<\/span><\/td>\nEx\/Em=535\/615 nm.<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTA1030<\/a><\/span><\/td>\nLDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9\/<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		\n

      \u00a0OD 565 nm<\/span><\/p>\n

      10,00-100,0000 cells per well<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>BMD00064-50UG<\/a><\/span><\/td>\nCalcein AM\u9223\u9ec3\u7da0\u7d20\u6d3b\u7d30\u80de\u87a2\u5149\u67d3\u5291<\/a><\/span><\/td>\n50ug<\/span><\/td>\n\n

      \u03bbEX\/\u03bbEm: 494\/517 nm (pH 8) (calcein)<\/span><\/p>\n

      \u00a0<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      \u00a0\u7d30\u80de\u51cb\u4ea1&\u7d30\u80de\u67d3\u8272 (8)<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
      \u7522\u54c1\u8ca8\u865f<\/span><\/strong><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/strong><\/td>\n\u53cd\u61c9<\/span><\/strong><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/strong><\/td>\n<\/tr>\n
      \n

      \u8ca8\u865f\u00a0KTA2010<\/a><\/span><\/p>\n

      \u00a0<\/span><\/td>\n

      		TUNEL\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n50\u500b\u53cd\u61c9<\/span><\/td>\n\u87a2\u5149\u9176\u6a19\u5100\u3001\u87a2\u5149\u986f\u5fae\u93e1\u6216\u6d41\u5f0f\u7d30\u80de\u5100\u3002FITC\u901a\u9053\uff08Ex \/ Em = 490nm \/ 520 nm\uff09<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTA0002<\/a><\/span><\/td>\nAnnexin V-AbFluor\u2122488\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n50\u500b\u53cd\u61c9<\/span><\/td>\nAnnexin V- AbFluor\u2122 488: Abs\/Em = 491\/517 nm; PI: Abs\/Em = 535\/617 nm (with DNA)<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTA4001<\/a><\/span><\/td>\nJC-1<\/u><\/strong>\u7dda\u7c92\u9ad4\u819c\u96fb\u4f4d\u6e2c\u5b9a\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/span><\/td>\n20\u500b\u53cd\u61c9<\/span><\/td>\n\n

      \uff08\u805a\u7d50\u7684JC-1\uff0cEx \/ Em = 585\/590 nm\uff09\u767c\u51fa\u7da0\u8272\u87a2\u5149\uff08JC-1\u55ae\u9ad4\uff0cEx \/ Em = 510\/527 nm\uff09<\/span><\/p>\n

      JC-1 Stain<\/span><\/p>\n

      CCCP (10 mM)\u00a0 Assay Buffer (5\u00d7)<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>BMD00063<\/a><\/span><\/td>\nDAPI Staining Solution<\/a><\/span><\/td>\n10MG<\/span><\/td>\n\u03bbEx\/\u03bbEm: 358\/461 nm (with DNA)<\/span><\/td>\n<\/tr>\n
      \u00a0KTD102-EN<\/a><\/span><\/td>\n\u51cb\u4ea1\u7d30\u80de\u5206\u6790(Annexin V\u6cd5\u548cTUNEL\u6cd5)<\/a><\/span><\/td>\n40\u500b\u5206\u6790\/20\u500b\u5206\u6790<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTC4100<\/a><\/span><\/td>\nPro\u6d3b\u7d30\u80de\u5fae\u7ba1\u86cb\u767d\u67d3\u8272<\/a><\/span><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/span><\/td>\nEx\/Em = 500\/520 nm<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTC4001<\/a><\/span><\/td>\nAbbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a><\/span><\/td>\n100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/span><\/td>\nEx\/Em = 484\/501 nm<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTC4003<\/a><\/span><\/td>\nTraKine\u2122\u7c92\u7dda\u9ad4\u67d3\u8272<\/a><\/span><\/td>\n100\u500b\u53cd\u61c9<\/span><\/td>\nEx \/ Em = 490\/523 nm\u589e\u6b96(proliferating)\u548c\u975e\u589e\u6b96\u7d30\u80de(non-proliferating cells)<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      DNA Damege\u640d\u50b7\/\u7d30\u80deStress (8)<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
      \u7522\u54c1\u8ca8\u865f<\/span><\/strong><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/strong><\/td>\n\u53cd\u61c9<\/span><\/strong><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/strong><\/td>\n<\/tr>\n
      \u8ca8\u865fKTB1050<\/a><\/span><\/td>\n\u8102\u8cea\u904e\u6c27\u5316\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a>(MDA)<\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\n\n

      OD 532 nm<\/span><\/p>\n

      1-100 \u00b5M\/L<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>KTB1910<\/a><\/span><\/td>\n\u6d3b\u6027\u6c27\uff08ROS\uff09\u6aa2\u6e2c\u5206\u6790\u8a66\u5291\u76d2<\/a><\/span><\/td>\n50\u500b\u53cd\u61c9\/100\u500b\u53cd\u61c9<\/span><\/td>\nDCFH-DA (10 mM)<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTB1400<\/a><\/span><\/td>\n\u4e00\u6c27\u5316Dan\uff08NO\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9\/<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		\n

      colorimetric (540 nm)<\/span><\/p>\n

      \u6aa2\u6e2c\u7bc4\u570d\uff1a1-100 \u00b5M<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>KTB1080<\/a><\/span><\/td>\n\u8d85\u6c27\u9670\u96e2\u5b50\u6e05\u9664\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9\/<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		Superoxide anion scavenging rate D% =\uff08\u2206\u2206ODControl – \u2206\u2206ODSample\uff09\u00f7 \u2206\u2206ODControl \u00d7100%<\/span><\/td>\n<\/tr>\n
      \u00a0\u8ca8\u865f<\/u><\/strong>\u00a0KTB1091<\/a><\/span><\/td>\n\u7fa5\u57fa\u81ea\u7531\u57fa\u6e05\u9664\u80fd\u529b\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9\/<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		\u5438\u6536\u5149 520nm<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTB1041<\/a><\/span><\/td>\nCheKine\u2122 \u904e\u6c27\u5316\u6c2b (H2O2) \u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      96\u500b\u53cd\u61c9\/<\/span><\/p>\n

      480\u500b\u53cd\u61c9<\/span><\/td>\n

      		\n

      OD 580 nm<\/span><\/p>\n

      : 1 \u00b5M-100 \u00b5M<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865fKTB1210<\/a><\/span><\/td>\nCheKine\u2122 \u8d85\u6c27\u9670\u96e2\u5b50\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9\/<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		\n

      absorption peak at 540nm<\/span><\/p>\n

      0.0125-0.5 mg\/mL<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865fKTB1020<\/a><\/span><\/td>\nNAD\/NADH \u5206\u6790\u8a66\u5291\u76d2<\/a><\/span><\/td>\n48\u500b\u53cd\u61c9<\/span><\/td>\n\n

      OD565 nm<\/span><\/p>\n

      96 \u5b54\u677f\u6e2c\u5b9a\u4e2d\u7684\u6aa2\u6e2c\u9650\u503c\u70ba 0.78 \u03bcM \u7dda\u6027\u6700\u9ad8 50 \u03bcM NAD+\/NADH\u3002<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      \u6297\u6c27\u5316\u5206\u6790\u8a66\u5291 (8)<\/span><\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
      \u7522\u54c1\u8ca8\u865f<\/span><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/td>\n\u53cd\u61c9<\/span><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTB1030<\/a><\/span><\/td>\n\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08SOD\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		200-3.13 U\/ml<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTB1040<\/a><\/span><\/td>\nCatalase\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\n\n

      OD 540 nm<\/span><\/p>\n

      2-75 \u00b5M<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>KTB1500<\/a><\/span><\/td>\n\u7e3d\u6297\u6c27\u5316\u5291\u80fd\u529bTCA\u5206\u6790<\/a><\/span><\/td>\n\n

      96\u500b\u53cd\u61c9\/<\/span><\/p>\n

      480\u500b\u53cd\u61c9<\/span><\/td>\n

      		\n

      593 nm<\/span><\/p>\n

      \u5075\u6e2c\u7bc4\u570d: 0.15-3 mM<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>\u00a0KTB1660<\/a><\/span><\/td>\n\u786b\u6c27\u9084\u86cb\u767d\u904e\u6c27\u5316\u7269\u9176\uff08TPX\uff09\u6aa2\u6e2c\u00a0<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9\/<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		240nm<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTB1600<\/a><\/span><\/td>\n\u7a40\u80f1\u7518\u80bd(Glutathione)\u6aa2\u6e2c<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		412 nm \u00a02-200 \u00b5g\/mL<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTB3010<\/a><\/span><\/td>\nCheKine\u2122 \u82b1\u9752\u7d20\u9084\u539f\u9176\uff08ANR\uff09\u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\nabsorption at 340 nm<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTB1530<\/a><\/span><\/td>\n\u9ec3\u916e\u985e\u5316\u5408\u7269\u6aa2\u6e2c\u5957\u7d44<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		\n

      absorption peak at 502 nm<\/span><\/p>\n

      0.156-10 mg\/g<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865fKTB1140<\/a><\/span><\/td>\n\u591a\u915a\u6c27\u5316\u9176\u6aa2\u6e2c\u5957\u7d44<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		\n

      \u00a0absorption peak at 525 nm<\/span><\/p>\n

      \u00a0<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      \u81bd\u56fa\u9187\u4ee3\u8b1d\/\u4ee3\u8b1d\u8a66\u9a57 (8)<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
      \u7522\u54c1\u8ca8\u865f<\/span><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/td>\n\u53cd\u61c9<\/span><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTB1510<\/a><\/span><\/td>\nCheKine\u2122\u5c3f\u9178\uff08UA\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9\/<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		\n

      \u00a0\u5438\u6536\u5149505 nm<\/span><\/p>\n

      Standard: 5 \u03bcmol\/mL.<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>KTB1300<\/a><\/span><\/td>\nGlucose\u8461\u8404\u7cd6\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\n\n

      \u00a0\u5438\u5149\u503c630nm<\/span><\/p>\n

      4- 300 mg\/dL)<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865fKTB1340<\/a><\/span><\/td>\nCheKine\u2122 \u809d\u91a3\/\u7cd6\u539f<\/u><\/strong>\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTB2220<\/a><\/span><\/td>\nTC\u7e3d\u81bd\u56fa\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9\/<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		\n

      500nm Standard: 5 \u03bcmol\/mL<\/span><\/p>\n

      \u00a0<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865fKTB2250<\/a><\/span><\/td>\nCheKine\u2122 \u5fae\u91cf\u9ad8\u5bc6\u5ea6\u8102\u86cb\u767d\u81bd\u56fa\u9187 (HDL-C) \u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9\/<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		\n

      Absorption peak at 500nm<\/span><\/p>\n

      Detection range: 0.078-5 mmol\/L(The Detection range corresponds to the standard, and the actual content of sample is 0.156-10 mmol\/L\uff09<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865fKTB2260<\/a><\/span><\/td>\nCheKine\u2122 \u5fae\u91cf\u4f4e\u5bc6\u5ea6\u8102\u86cb\u767d\u81bd\u56fa\u9187 (LDL-C) \u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9\/<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		\n

      Absorption peak at 500nm<\/span><\/p>\n

      Detection range: 0.078-5 mmol\/L(The Detection range corresponds to the standard, and the actual content of sample is 0.117-7.5 mmol\/L\uff09<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865fKTB1390<\/a><\/span><\/td>\nCheKine\u2122 \u6fb1\u7c89\u5206\u652f\u9176 (SBE) \u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9\/<\/span><\/p>\n

      \u00a0<\/span><\/td>\n

      		absorption at 660 nm<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTB1070<\/a><\/span><\/td>\nCheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\n\n

      OD 450 nm<\/span><\/p>\n

      \u7dda\u6027\u7bc4\u570d15.6 -500 mU\/<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      \u751f\u5316\u6aa2\u6e2c\u8a66\u5291\u76d2 (8)<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
      \u7522\u54c1\u8ca8\u865f<\/strong><\/td>\n\u7522\u54c1\u54c1\u9805<\/strong><\/td>\n\u53cd\u61c9<\/strong><\/td>\n\u6e2c\u91cf\u5100\u5668<\/strong><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTB1700<\/a><\/span><\/td>\n\u7d44\u7e54\u548c\u8840\u6db2\u9e7c\u6027\u78f7\u9178\u9176\uff08\u00a0Alkaline Phosphatase, AKP \/ ALP\uff09\u6bd4\u8272\u6e2c\u5b9a<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9\/<\/span><\/td>\n\u5438\u6536\u5149510 nm<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTB1542<\/a><\/span><\/td>\nTannase(TAN) \u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/td>\n48\u500b\u53cd\u61c9\/<\/span><\/td>\n\n

      absorbance at 270 nm<\/span><\/p>\n

      0.3125-20 \u03bcmoL\/mL<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865fKTB1541<\/a><\/span><\/td>\nCheKine\u2122 \u55ae\u5be7\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | CheKine\u2122 Tannin Colorimetric Assay Kit<\/a><\/span><\/td>\n48\u500b\u53cd\u61c9\/<\/span><\/td>\n\n

      absorbance at 760 nm<\/span><\/p>\n

      0.0156-1 mg\/mL<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865fKTB1290<\/a><\/span><\/td>\nCheKine\u2122 \u70cf\u982d\u9178\u9176 (ACO) \u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n48\u500b\u53cd\u61c9\/<\/span><\/td>\nabsorption at 340 nm.<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTB1560<\/a><\/span><\/td>\n\u9187\u91af\u57fa\u8f49\u79fb\u9176\uff08AAT\uff09\u6d3b\u6027\u6bd4\u8272\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9\/<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		absorption at 412 nm.<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTB1070<\/a><\/span><\/td>\nCheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\n\n

      OD 450 nm<\/span><\/p>\n

      \u7dda\u6027\u7bc4\u570d15.6 -500 mU\/<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865fKTB1380<\/a><\/span><\/td>\n\u03b2-\u6fb1\u7c89\u9176\u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a<\/a><\/span><\/td>\n48\u500b\u53cd\u61c9<\/span><\/td>\n\n

      absorption peak at 540 nm<\/span><\/p>\n

      0.0156-1 mg\/mL<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>KTB1310<\/a><\/span><\/td>\n\u8461\u8404\u7cd6\u6c27\u5316\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a><\/span><\/td>\n48\u500b\u53cd\u61c9<\/span><\/td>\n\n

      OD 580 \u4e0b\u6e2c\u91cf<\/span><\/p>\n

      \u5075\u6e2c\u7bc4\u570d:\u00a00.05 – 2 U\/L<\/u><\/strong><\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      \u767c\u708e\u53cd\u61c9ELISA\u5206\u6790<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
      \u7522\u54c1\u8ca8\u865f<\/strong><\/td>\n\u7522\u54c1\u54c1\u9805<\/strong><\/td>\n\u53cd\u61c9<\/strong><\/td>\n\u6e2c\u91cf\u5100\u5668<\/strong><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTE7013<\/a><\/span><\/td>\nIL-6 ELISA Kit<\/u><\/strong>\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/span><\/td>\n\n

      96\u500b\u53cd\u61c9\/<\/span><\/p>\n

      \u00a0<\/span><\/td>\n

      		\n

      450 nm<\/span><\/p>\n

      \u5075\u6e2c\u7bc4\u570d: 250pg\/mL-16000 pg\/mL, \u6700\u4f4e\u9748\u654f\u5ea6100 pg\/mL<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>KTE6032<\/a><\/span><\/td>\nTNF<\/u><\/em>–\u03b1<\/u><\/em>\u00a0Alpha\u578b\u816b\u7624\u58de\u6b7b\u56e0\u5b50 ELISA\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      96\u500b\u53cd\u61c9\/<\/span><\/p>\n

      \u00a0<\/span><\/td>\n

      		\n

      450 nm<\/span><\/p>\n

      7.8 pg\/ml-500 pg\/ml<\/u><\/strong><\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>KTE6033<\/a><\/span><\/td>\nVEGF\u8840\u7ba1\u5167\u76ae\u751f\u9577\u56e0\u5b50ELISA\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/td>\n\n

      96\u500b\u53cd\u61c9\/<\/span><\/p>\n

      \u00a0<\/span><\/td>\n

      		\n

      450 nm<\/span><\/p>\n

      \u5075\u6e2c\u7bc4\u570d:\u00a031.25 pg\/mL-2000 pg\/mL<\/u><\/strong><\/span><\/td>\n<\/tr>\n

      KTE7013<\/span><\/td>\nIL-22 ELISA\u8a66\u5291\u76d2<\/span><\/td>\n\n

      96\u500b\u53cd\u61c9\/<\/span><\/p>\n

      \u00a0<\/span><\/td>\n

      		\u00a0<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTE7003<\/a><\/span><\/td>\nIFN-\u03b3\uff08\u5e72\u64fe\u7d20-\u03b3\uff09ELISA\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      96\u500b\u53cd\u61c9\/<\/span><\/p>\n

      \u00a0<\/span><\/td>\n

      		31.25 pg\/ml- 2000 pg\/ml<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTE6019<\/a><\/span><\/td>\nIL-10 ELISA\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      96\u500b\u53cd\u61c9\/<\/span><\/p>\n

      \u00a0<\/span><\/td>\n

      		2.35 pg\/ml-150 pg\/ml<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTE7002<\/span><\/td>\nGM-CSF ELISA\u8a66\u5291\u76d2<\/span><\/td>\n96\u500b\u53cd\u61c9\/<\/span><\/td>\n7.8 pg\/mL-500 pg\/mL<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTE7005<\/a><\/span><\/td>\n\u767d\u4ecb\u7d20-1\u03b2ELISA\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      96\u500b\u53cd\u61c9\/<\/span><\/p>\n

      96 X5\u76e4<\/span><\/td>\n

      		\n

      Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.<\/span><\/p>\n

      \u5075\u6e2c\u7bc4\u570d:\u00a015.6 pg\/ml-1000 pg\/ml<\/u><\/strong><\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      \u91cd\u7d44\u86cb\u767d&\u55ae\u591a\u682a\u6297\u9ad4<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
      \u7522\u54c1\u8ca8\u865f<\/span><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/td>\n\u53cd\u61c9<\/span><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>PRP1012<\/a><\/span><\/td>\nHuman IL-6 protein\u91cd\u7d44\u86cb\u767d\u00a0<\/u><\/strong><\/a><\/span><\/td>\n5ug<\/span><\/td>\nhuman IL-6 (NP_000591.1) (Val 30-Met 212)\/ E. coli<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>PRP100273<\/a><\/span><\/td>\nHuman IL-4 protein\u91cd\u7d44\u86cb\u767d<\/u><\/strong><\/a><\/span><\/td>\n20ug<\/span><\/td>\nED50 for this effect is 0.05-0.2 ng\/mL. The specific activity of Recombinant Human IL-4 is approximately 3.2 \u00d7 10E4 IU\/\u03bcg.<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>PRP100159<\/a><\/span><\/td>\nHuman EGF protein\u91cd\u7d44\u86cb\u767d<\/u><\/strong><\/a><\/span><\/td>\n100ug<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>PRP1012<\/a><\/span><\/td>\nHuman IL-6 protein\u91cd\u7d44\u86cb\u767d\u00a0<\/u><\/strong><\/a><\/span><\/td>\n5ug<\/span><\/td>\nhuman IL-6 (NP_000591.1) (Val 30-Met 212)\/ E. coli<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>ABP52783<\/a><\/span><\/td>\nGAPDH Polyclonal Antibody<\/a><\/span><\/td>\n30ul<\/span><\/td>\nHuman, Mouse, Rat\u3002\u53ef\u61c9\u7528\u65bcELISA, IHC-P, WB\u5be6\u9a57\u3002<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fA21010<\/span><\/td>\nHRP, Goat Anti-Mouse IgG<\/span><\/td>\n100ul<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fA21020<\/span><\/td>\nHRP, Goat Anti-Rabbit IgG<\/span><\/td>\n100ul<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fA23220<\/span><\/td>\nDylight 488, Goat Anti-Rabbit IgG<\/span><\/td>\n100ul<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      \u6297\u9ad4\u86cb\u767d\u7d14\u5316&\u5176\u5b83<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
      \u7522\u54c1\u8ca8\u865f<\/span><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/td>\n\u53cd\u61c9<\/span><\/td>\n\u7279\u9ede<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTP2070<\/a><\/span><\/td>\n\u6297\u9ad4\u7d14\u5316\u8a66\u5291\u76d2\uff08\u86cb\u767d A\/G\uff09<\/a><\/span><\/td>\n1ml<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
      \u00a0\u8ca8\u865fBMR2001<\/a><\/span><\/td>\nHis-Tag Ni-NTA Resin\u6a39\u8102<\/a><\/span><\/td>\n5ml<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>\u00a0KTD105-EN<\/a><\/span><\/td>\nIP\/Co-IP Toolkit (Agarose)\u00a0<\/a><\/span><\/td>\n20\u500b\u53cd\u61c9<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTD3001<\/a><\/span><\/td>\n\u00a0BCA\u86cb\u767d\u5b9a\u91cf\u8a66\u5291\u76d2<\/a><\/span><\/td>\n500\u500b\u53cd\u61c9<\/span><\/td>\n\n

      OD 562 nm<\/span><\/p>\n

      \u76f8\u5bb9\u6027 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80<\/span><\/p>\n

      50 to 1000 \u00b5g\/ml.<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>KTP2140<\/a><\/span><\/td>\nPurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u6a39\u8102(\u5957\u7d44)<\/a><\/span><\/td>\n1ml\u00d75<\/span><\/td>\nMore than 2,000,000 EU\/mL to eliminate >99% of endotoxins.<\/span><\/td>\n<\/tr>\n
      BMD0062<\/span><\/td>\nHoechst 33342<\/span><\/td>\n10MG<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
      BMU104-EN<\/span><\/td>\n\u589e\u5f37\u578bAntifade\u5c01\u7247\u5291<\/span><\/td>\n10ml<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>BMS0050<\/a><\/span><\/td>\n\u6b63\u5e38\u5c71\u7f8a\u8840\u6e05<\/a><\/span><\/td>\n10ml<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      \u7d30\u80de\u57f9\u990a\u76f8\u95dc<\/span><\/strong><\/span><\/p>\n\n\n\n\n
      \u7522\u54c1\u8ca8\u865f<\/span><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/td>\n\u53cd\u61c9<\/span><\/td>\n\u7279\u9ede<\/td>\n<\/tr>\n
      BMU108-EN<\/strong><\/a><\/span><\/td>\nSuperKine\u2122 \u7121\u8840\u6e05\/\u7121\u86cb\u767d\u7d30\u80de\u51b7\u51cd\u57f9\u990a\u57fa<\/a><\/span><\/td>\n100ML\u00a0<\/span><\/td>\n\u6210\u5206\u6e05\u6670\uff0c\u7121\u8840\u6e05\/\u7121\u86cb\u767d\uff0c\u907f\u514d\u75c5\u6bd2\u3001\u7d30\u83cc\u3001\u652f\u539f\u9ad4\u7b49\u6c61\u67d3<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

       <\/p>\n","protected":false},"excerpt":{"rendered":"

      \u5f57\u661f\u6aa2\u6e2c\u8a66\u5291\u76d2\uff083 well\uff09| CometAssay Kit\u8ca8\u865fKTA3040\u00a0\u00a075\u500b\u53cd\u61c9(3-well) […]<\/p>\n","protected":false},"author":3,"featured_media":41131,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262],"tags":[5570],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/41121"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=41121"}],"version-history":[{"count":8,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/41121\/revisions"}],"predecessor-version":[{"id":49362,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/41121\/revisions\/49362"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/41131"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=41121"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=41121"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=41121"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}