{"id":41101,"date":"2023-04-14T18:06:25","date_gmt":"2023-04-14T10:06:25","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=41101"},"modified":"2023-06-19T18:14:20","modified_gmt":"2023-06-19T10:14:20","slug":"rscript-cdna-%e5%90%88%e6%88%90%e8%a9%a6%e5%8a%91%e7%9b%92200-u%ce%bcl-rscript-cdna-synthesis-kit%e8%b2%a8%e8%99%9frk001-0050","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=41101","title":{"rendered":"RScript cDNA \u5408\u6210\u8a66\u5291\u76d2(200 U\/\u03bcl) | RScript cDNA Synthesis Kit\u8ca8\u865fRK001-0050"},"content":{"rendered":"

RScript cDNA \u5408\u6210\u8a66\u5291\u76d2(200 U\/\u03bcl)\u00a0 | RScript cDNA Synthesis Kit\u8ca8\u865fRK001-0050<\/a>\u00a0<\/span><\/strong><\/p>\n

RScript cDNA Synthesis Kit (50 Rxns)<\/p>\n

Categories:\u00a0RNA Reagent<\/a><\/p>\n

\u4e00\u3001\u7522\u54c1\u4ecb\u7d39<\/strong><\/span><\/p>\n

RScript cDNA \u5408\u6210\u8a66\u5291\u76d2(RScript cDNA Synthesis Kit, RK001-0050) \u5c08\u9580\u7528\u65bc\u7814\u7a76\u548c\u8a3a\u65b7\u61c9\u7528\uff0c\u53ef\u6eff\u8db3\u6240\u6709\u7684 cDNA \u5408\u6210\u9700\u6c42\uff1b\u514b\u670d\u5bec\u6eab\u5ea6\u7bc4\u570d\u5167\u6700\u5177\u6311\u6230\u6027\u7684\u4e8c\u7d1a RNA \u7d50\u69cb(secondary RNA structures)\u3002\u5957\u7d44\u5167\u5305\u542b\u65b0\u4e00\u4ee3\u5de5\u7a0b\u91cd\u7d44 M-MLV \u9006\u8f49\u9304\u9176(M-MLV reverse transcriptase)\uff0c\u5177\u6709\u6539\u9032\u7684\u71b1\u7a69\u5b9a\u6027(\u00a0improved thermostability)\u3001\u6301\u7e8c\u5408\u6210\u80fd\u529b(robustness)\u3001\u7a69\u5065\u6027\u3001\u6700\u4f73 cDNA \u7522\u91cf(\u00a0optimal cDNA yields)\u3001Moloney \u5c0f\u9f20\u767d\u8840\u75c5\u75c5\u6bd2\u9006\u8f49\u9304\u9176 (M-MLV RT) \u7f3a\u4e4f DNA \u6838\u9178\u5167\u5207\u9176\u6d3b\u6027\uff0c\u5177\u6709\u8f03\u4f4e\u7684 RNase H \u6d3b\u6027\u3002\u662f\u6700\u901a\u7528\u7684\u4e16\u754c\u4e0a\u7684\u9006\u8f49\u9304\u9176\uff0c\u4e0d\u50c5\u53ef\u4ee5\u7c21\u55ae\u5730\u6eff\u8db3\u5e38\u898f cDNA \u5408\u6210\u8981\u6c42\uff0c\u800c\u4e14\u5373\u4f7f\u5c0d\u65bc\u624b\u982d\u6700\u5177\u6311\u6230\u6027\u7684 RNA \u6a23\u54c1\u4e5f\u80fd\u5be6\u73fe\u5353\u8d8a\u7684\u6027\u80fd\u3002<\/p>\n

\"\"<\/p>\n

Reverse transcription of a 330 bp fragment CCD1A RNA using RScript cDNA Synthesis Kit to carry this experiment. The PCR data shows that RScript cDNA Synthesis Kit have a stable and superior performance at higher temperatures.<\/p>\n

\"\"
\n\"\u64cd\u4f5c\u624b\u518a\"<\/a><\/p>\n

\u4e8c\u3001\u7522\u54c1\u8aaa\u660e<\/strong><\/span><\/p>\n

\"\"<\/p>\n

Template Total RNA, synthetic RNA transcript or poly(A)+mRNA, or the RNA should be avoided for cross-contamination with DNA.<\/p>\n

Primer Selection Primer amounts recommended for efficient cDNA synthesis are 2.5 uM of oligo(dT) (anneal to the 3\u2019-poly(A) + mRNA) or 2.5 ng\/ul of random primers (anneal at non-specific sites of RNA templates), or 2 uM of genespecific primers per 20 ul reaction.<\/p>\n

\u4e09\u3001RScript cDNA \u5408\u6210\u64cd\u4f5c\u65b9\u6cd5 (\u82e5\u6709\u4e0d\u540c\u4f9d\u539f\u5ee0\u7db2\u7ad9\u516c\u544a\u70ba\u4e3b)\u00a0<\/strong><\/span><\/p>\n

\"BIO-HELIX<\/p>\n

Reaction Setup cDNA Synthesis <\/span><\/p>\n

1. For each 20 ul cDNA synthesis reaction, assemble the following in a PCR tube. Keep it on ice just prior to use.<\/p>\n

\"\"<\/p>\n

*RNA template, primer and 2X Sharp Reaction Mix need to be premixed and heated at 65o C for 5 minutes in advance. After RNA has been pre-heated and incubated on ice bath at least 1 minute, add other components according to the table.<\/p>\n

2. Mix the reaction solution gently by pipetting.<\/p>\n

3. Cap the tubes and place them in the temperature-controlled water bath or heat blocks. Incubate the tubes at 55o C for 50 mins for the extension step. The 42\u00b0C \u2013 60\u00b0C temperature range may be the optimal temperature for extension.<\/p>\n

4. The reaction tube from the Step 3 must be incubated at 70o C for 15 minutes for inactivating the Reverse Transcriptase before amplification.<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n
RK001-0050
\ncDNA\u5408\u6210KIT<\/td>\n		RScript\u2122 cDNA Synthesis Kit
\n(\u65b0\u4e00\u4ee3\u5de5\u7a0b\u91cd\u7d44 M-MLV \u9006\u8f49\u9304\u9176(M-MLV reverse transcriptase))
\nRScript cDNA \u5408\u6210\u8a66\u5291\u76d2(200 U\/\u03bcl)\u00a0<\/span><\/a><\/strong><\/td>\n		50 rxns<\/td>\n<\/tr>\n
RT001-0050
\nRT\u9006\u8f49\u9304\u9176<\/td>\n		RScript\u2122 Reverse Transcriptase 10,000U
\n(\u65b0\u4e00\u4ee3\u5de5\u7a0b\u91cd\u7d44 M-MLV \u9006\u8f49\u9304\u9176(M-MLV reverse transcriptase))
\n\u9006\u8f49\u9304\u9176 RScript Reverse Transcriptase<\/span><\/strong><\/a><\/td>\n		50 rxns
\n10,000U\/50\u03bcl<\/td>\n<\/tr>\n
RT001-0250
\nRT\u9006\u8f49\u9304\u9176<\/td>\n		RScript\u2122 Reverse Transcriptase 50,000U
\n(\u65b0\u4e00\u4ee3\u5de5\u7a0b\u91cd\u7d44 M-MLV \u9006\u8f49\u9304\u9176(M-MLV reverse transcriptase))
\n\u9006\u8f49\u9304\u9176RScript Reverse Transcriptase<\/span><\/strong><\/a><\/td>\n		250 rxns
\n50,000U\/250\u03bcl<\/td>\n<\/tr>\n
RI001-0125
\nRNase Inhibitor<\/td>\n		RIBOAssure\u2122 RNase Inhibitor 5,000U
\n(\u6291\u5236\u771f\u6838\u9176\u5982 RNase A\u3001RNase B \u548c RNase C \u6240\u8868\u73fe\u51fa\u7684\u6838\u7cd6\u6838\u9178\u9176 (RNase) \u6d3b\u6027)
\nRNase \u00a0\u6291\u5236\u5291<\/span><\/a><\/td>\n		125 rxns
\n5,000U\/125\u03bcl<\/td>\n<\/tr>\n
QPR01-0100
\n(Probe)<\/td>\n		PanProbes\u2122 One-Step RT-qPCR Kit (Probe)
\n(\u65b0\u4e00\u4ee3\u5de5\u7a0b\u91cd\u7d44 M-MLV \u9006\u8f49\u9304\u9176(M-MLV reverse transcriptase))
\n\u5f37\u5927\u7684 RNase \u6291\u5236\u5291\u6df7\u5408\u7269(RNase inhibitors mix)
\n\u589e\u5f37\u7684\u6297\u9ad4\u4ecb\u5c0e\u7684\u71b1\u555f\u52d5 DNA \u805a\u5408\u9176
\n(\u91dd\u5c0d\u57fa\u65bc\u63a2\u91dd\u7684real-time PCR \u9032\u884c\u4e86\u512a\u5316)
\n\u4e00\u6b65\u9a5f RT-qPCR <\/span><\/strong>\u8a66\u5291\u76d2<\/strong><\/span><\/a><\/td>\n		100 reactions
\n(20 \u03bcl vol)<\/td>\n<\/tr>\n
QPD01-0100
\n(Probe)<\/td>\n		PanProbes\u2122 Universal qPCR MasterMix
\n(\u91dd\u5c0d\u57fa\u65bcprobe- PCR (probe-based real-time PCR )\u9032\u884c\u4e86\u512a\u5316)
\n\u589e\u5f37\u7684\u6297\u9ad4\u4ecb\u5c0e\u7684\u71b1\u555f\u52d5 DNA \u805a\u5408\u9176
\nPanProbes<\/span><\/strong>\u2122 Universal qPCR<\/strong>\u9810\u6df7\u6db2<\/strong><\/span><\/a><\/td>\n		100 reactions
\n(20 \u03bcl vol)<\/td>\n<\/tr>\n
QSR01-0100
\n(SYBR\u00ae Green)<\/td>\n		PanGreen\u2122 One-Step RT-qPCR Kit (SYBR\u00ae Green)
\n(\u65b0\u4e00\u4ee3\u5de5\u7a0b\u91cd\u7d44 M-MLV \u9006\u8f49\u9304\u9176(M-MLV reverse transcriptase))
\n\u5f37\u5927\u7684 RNase \u6291\u5236\u5291\u6df7\u5408\u7269(RNase inhibitors mix)
\n\u589e\u5f37\u7684\u6297\u9ad4\u4ecb\u5c0e\u7684\u71b1\u555f\u52d5 DNA \u805a\u5408\u9176
\nUniversal SYBR\u00ae Green Master Mix\u00a0 2 \u500d\u6fc3\u7e2e\u7684\u5373\u7528\u578b\u6df7\u5408\u6db2 Master Mix
\n\u4e00\u500b\u6b65\u9a5fRT-qPCR <\/span><\/strong>\u8a66\u5291\u76d2PanGreen<\/strong><\/span><\/a><\/td>\n		100 reactions
\n(20 \u03bcl vol)<\/td>\n<\/tr>\n
QSD01-0100
\nSYBR\u00ae Green)<\/td>\n		PanGreen\u2122 Universal SYBR\u00ae Green Mastermix (SYBR\u00ae Green)
\n(2 \u500d\u6fc3\u7e2e\u7684\u5373\u7528\u578b Master Mix \u6df7\u5408\u6db2)
\n\u589e\u5f37\u7684\u6297\u9ad4\u4ecb\u5c0e\u7684\u71b1\u555f\u52d5 DNA \u805a\u5408\u9176
\n\u5373\u7528\u578b qPCR <\/span><\/strong>\u6df7\u5408\u6db2PanGreen<\/strong><\/span><\/a><\/td>\n		100 reactions
\n(20 \u03bcl vol)<\/td>\n<\/tr>\n
PDR03-0100<\/td>\nTotal RNA Isolation Reagent
\n(Reagent Based) for the tissue, cultured animal and bacterial cells, blood, and serum.
\nTotal RNA <\/span><\/strong>\u5206\u96e2\u8a66\u5291 (Reagent Based<\/strong>\uff09<\/strong><\/span><\/a><\/td>\n		100 rxns<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/strong><\/a>\u00a0|\u00a0<\/span>\u00a0<\/strong>Finnpipette F3\u00a0<\/strong>\u5fae\u91cf\u5206\u6ce8\u5668<\/strong><\/a>\u00a0|<\/span>\u00a0\u00a0<\/strong>\u8a66\u7ba1\u632f\u76ea\u5668 Vortex Mixer\u00a0<\/strong><\/a>\u00a0|\u00a0<\/span>\u00a0<\/strong>Genereach<\/strong>\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/strong><\/a>\u00a0\u00a0|\u00a0<\/span>\u00a0\u00a0<\/strong>ELISA<\/strong>\u5100\u5668<\/strong><\/a><\/span><\/p>\n

\"\"<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716<\/p>\n

\"BIO-HIDEX\"<\/p>\n

bio-helix.com<\/a><\/span><\/p>\n

Bio-Helix\u6210\u7acb\u65bc2007\u5e74\uff0c\u5c08\u6ce8\u65bc\u70ba\u5168\u7403\u751f\u547d\u79d1\u5b78\u7814\u7a76\u4eba\u54e1\u63d0\u4f9b\u6700\u597d\u7684\u751f\u7269\u8a66\u5291\u3002\u5305\u62ec DNA \u6a19\u8a18\u7269\u3001\u86cb\u767d\u8cea\u6a19\u8a18\u7269\u3001\u9810\u88fd\u86cb\u767d\u8cea\u51dd\u81a0\u3001\u6838\u9178\u7d14\u5316\u3001PCR\u8a66\u5291\u7b49\u3002\u8207\u4e16\u754c\u5404\u5730\u5be6\u9a57\u5ba4\u7684\u79d1\u5b78\u5bb6\u5011\u4e00\u8d77\u52aa\u529b\uff0c\u4e0d\u65b7\u63d0\u9ad8\u8cea\u91cf\uff0c\u958b\u59cb\u8d0f\u5f97\u5ba2\u6236\u7684\u4fe1\u4efb\u548c\u8a55\u50f9\u3002\u54c1\u724c\u904d\u5e03\u56db\u5341\u591a\u500b\u570b\u5bb6\uff0c\u5df2\u6210\u70ba\u751f\u547d\u79d1\u5b78\u5be6\u9a57\u5ba4\u503c\u5f97\u4fe1\u8cf4\u548c\u6b61\u8fce\u7684\u8a66\u5291\u54c1\u724c\u3002Bio-Helix\u500b\u9858\u666f\uff0c\u5c31\u662f\u6709\u4e00\u5929\u6211\u5011\u7684\u58fd\u547d\u53ef\u4ee5\u5ef6\u9577\uff1b\u6240\u6709\u75be\u75c5\u7684\u65e9\u671f\u767c\u73fe\u548c\u96a8\u5f8c\u7684\u9069\u7576\u6cbb\u7642\u90fd\u53ef\u4ee5\u63d0\u9ad8\u751f\u6d3b\u8cea\u91cf\u3002\u4e2d\u570b\u53e4\u4eba\u8a8d\u70ba\uff0c\u826f\u91ab\u6cbb\u672a\u75c5\uff0c\u6cbb\u672a\u75c5\u3002\u5728 Bio-Helix \u4e2d\uff0c\u5275\u5efa\u4e86\u4e00\u500b\u65b0\u54c1\u724c LifeDirex \u4f86\u5be6\u73fe\u9019\u4e00\u9858\u666f\u3002 LifeDirex \u5c07\u5c08\u6ce8\u65bc\u70ba\u8a3a\u65b7\u5de5\u5177\u63d0\u4f9b\u6700\u597d\u548c\u6700\u5177\u5275\u65b0\u6027\u7684\u8a66\u5291\uff0c\u4ee5\u4fc3\u9032\u76e1\u53ef\u80fd\u65e9\u5730\u767c\u73fe\u75be\u75c5\u3002Bio-Helix\u4f7f\u547d\u662f\u63d0\u4f9b\u6700\u512a\u8cea\u7684\u7522\u54c1\u4e26\u958b\u767c\u65b0\u6280\u8853\uff0c\u5e6b\u52a9\u7814\u7a76\u4eba\u54e1\u627e\u5230\u5c0b\u6c42\u79d1\u5b78\u6210\u5c31\u7684\u89e3\u6c7a\u65b9\u6848\u3002\u6b64\u5916\uff0cBio-Helix\u8a8d\u70ba\u73fe\u5728\u662f\u9032\u5165\u9ad4\u5916\u8a3a\u65b7\u8a2d\u5099\uff08IVDD\uff09\u5e02\u5834\u7684\u6700\u4f73\u6642\u6a5f\u3002\u6211\u5011\u7684\u76ee\u6a19\u662f\u6210\u70ba\u7814\u7a76\u5be6\u9a57\u5ba4\u548cIVDD\u7684\u6700\u4f73\u89e3\u6c7a\u65b9\u6848\u63d0\u4f9b\u5546\u3002<\/p>\n

\u5728\u6539\u5584\u751f\u6d3b\u8cea\u91cf\u548c\u5be6\u73fe\u79d1\u5b78\u5353\u8d8a\u7684\u9858\u671b\u7684\u9a45\u4f7f\u4e0b\uff0cBio-Helix\u4ee5\u6700\u9ad8\u7684\u8aa0\u4fe1\u6a19\u6e96\u904b\u71df\u3002\u9084\u5c07\u9019\u7a2e\u7cbe\u795e\u5ef6\u4f38\u5230\u6211\u5011\u7684\u670d\u52d9\u548c\u5168\u7403\u50f9\u503c\u4e2d\uff0c\u4ee5\u63d0\u4f9b\u7d66\u4e16\u754c\u5404\u5730\u7684\u5ba2\u6236\u3002\u591a\u6a23\u5316\u7684\u54e1\u5de5\u968a\u4f0d\u548c\u5354\u4f5c\u7db2\u7d61\u5f62\u6210\u4e86\u516c\u53f8\u6587\u5316\u7684\u5b8c\u7f8e\u7d50\u5408\u3002 Bio-Helix \u4e0d\u50c5\u662f\u4e00\u5bb6\u6a5f\u6703\u5747\u7b49\u7684\u516c\u53f8\uff0c\u800c\u4e14\u9084\u52aa\u529b\u63d0\u4f9b\u53cb\u597d\u548c\u6210\u9577\u7684\u74b0\u5883\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
CAT#<\/td>\nNAME<\/td>\nSize<\/td>\n<\/tr>\n
BP001CU<\/td>\nBluPAD, LED Transilluminator, with mini Darkroom
\n(CE\/ETL, Patent No: M543442)<\/td>\n		Set<\/td>\n<\/tr>\n
PMI09-0500<\/td>\nIRIS9 Plus Prestained Protein Ladder
\n(9 pre-stained bands, 15-170 kDa)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
PMI11-0500<\/td>\nIRIS11 Prestained Protein Ladder
\n(11 pre-stained bands, 3-260 kDa)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
PMB01-0500<\/td>\nBlu10 Plus Prestained Protein Ladder
\n(10 pre-stained bands, 6.5-270 kDa)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
PMB10-0500<\/td>\nBlu10 Prestained Protein Ladder
\n(10 pre-stained bands, 11-180 kDa)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
PMB11-0500<\/td>\nBlu11 Prestained Protein Ladder
\n(11 pre-stained bands, 10-180 kDa)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
PMB12-0500<\/td>\nBlu12 Prestained Protein Ladder
\n(12 pre-stained bands, 11-245 kDa)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
PMB13-0500<\/td>\nBlu13 Prestained Protein Ladder
\n(13 pre-stained bands, 5-245 kDa)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
PMU12-0500<\/td>\nUNveil Unstained Protein Ladder, Broad Range
\n(12 unstained bands, 10-200 kDa)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
CCH321-B100ML<\/td>\nUltraScence Pico Plus Western Substrate
\n(Patent No: US10,711,185)<\/td>\n		100 ml
\n(50ml+50ml)<\/td>\n<\/tr>\n
CCH345-B100ML<\/td>\nUltraScence Pico Ultra Western Substrate
\n(Patent No: US10,711,185)<\/td>\n		100 ml
\n(50ml+50ml)<\/td>\n<\/tr>\n
CCH365-B100ML<\/td>\nUltraScence Femto Western Substrate
\n(Patent No: US10,711,185)<\/td>\n		100 ml
\n(50ml+50ml)<\/td>\n<\/tr>\n
CCH375-B100ML<\/td>\nUltraScence Femto Plus Western Substrate
\n(Patent No: US10,711,185)<\/td>\n		100 ml
\n(50ml+50ml)<\/td>\n<\/tr>\n
PS002-B500ML<\/td>\nCOOMASSIEnano Protein Staining Solution, 500ml<\/td>\n500 ml<\/td>\n<\/tr>\n
PS003-B500ML<\/td>\nPonceau S Protein Staining Solution<\/td>\n500 ml<\/td>\n<\/tr>\n
BS001-B500ML<\/td>\nOneStep Blocker
\nWestern Blocking Solution and Signal Enhancer<\/td>\n		500 ml<\/td>\n<\/tr>\n
TM001-B200ML<\/td>\nTMB ELISA Substrate<\/td>\n100 ml
\n(50ml+50ml)<\/td>\n<\/tr>\n
DM001-R500<\/td>\nBH 100bp DNA Ladder RTU
\n(11 bands, 100-1,500bps)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
DM003-R500<\/td>\nBH 100bp DNA Ladder H3 RTU
\n(12 bands, 100-3,000bps)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
DM012-R500<\/td>\nBH 50bp DNA Ladder RTU
\n(17 bands, 50-1,500bps)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
DM015-R500<\/td>\nBH 1Kb Plus DNA Ladder RTU
\n(13 bands, 100-10,000bps)<\/td>\n		500 \u03bcl<\/td>\n<\/tr>\n
DMF12-0100<\/td>\nOmniMARK 100 DNA Ladder RTU
\n(12 bands, 100-3,000bps, fluorescent dye)<\/td>\n		600 \u03bcl<\/td>\n<\/tr>\n
DMF13-0100<\/td>\nOmniMARK 1K DNA Ladder RTU
\n(13 bands, 100-10,000bps, fluorescent dye)<\/td>\n		600 \u03bcl<\/td>\n<\/tr>\n
LD011-1000<\/td>\nPrime Juice Preloading Fluorescent Stain<\/td>\n1 ml<\/td>\n<\/tr>\n
LD003-0500<\/td>\nNovel Green Plus (20,000X) DNA Staining Reagent<\/td>\n500 \u03bcl<\/td>\n<\/tr>\n
AGT002-0500<\/td>\nAgarose Tablets, 110 pcs<\/td>\n0.5g x 110<\/td>\n<\/tr>\n
MB755-0100<\/td>\nAgarose Powder 100g -Molecular Biology Grade<\/td>\n100 g<\/td>\n<\/tr>\n
MB755-0500<\/td>\nAgarose Powder 500g -Molecular Biology Grade<\/td>\n500 g<\/td>\n<\/tr>\n
MB101-0500<\/td>\nTaq DNA Polymerase<\/td>\n500U\/100\u03bcl<\/td>\n<\/tr>\n
DP001-0100<\/td>\nnanoTaq\u2122 Hot-Start DNA Polymerase<\/td>\n100 rxns
\n500U\/100\u03bcl<\/td>\n<\/tr>\n
DN001-0250<\/td>\n100 mM dNTP Set<\/td>\n4 x 250\u03bcl<\/td>\n<\/tr>\n
DN0010<\/td>\n10 mM dNTP Mix<\/td>\n1 ml<\/td>\n<\/tr>\n
MB200-P100<\/td>\n2X PCR SuperMix<\/td>\n100 rxns
\n2 x 1.25mL<\/td>\n<\/tr>\n
MBA01-0100<\/td>\nOmniPCR Supermix w Fluorescent dye<\/td>\n100 rxns
\n(2.5ml)<\/td>\n<\/tr>\n
RK001-0050<\/td>\nRScript\u2122 cDNA Synthesis Kit<\/td>\n50 rxns<\/td>\n<\/tr>\n
RT001-0050<\/td>\nRScript\u2122 Reverse Transcriptase 10,000U<\/td>\n50 rxns
\n10,000U\/50\u03bcl<\/td>\n<\/tr>\n
RT001-0250<\/td>\nRScript\u2122 Reverse Transcriptase 50,000U<\/td>\n250 rxns
\n50,000U\/250\u03bcl<\/td>\n<\/tr>\n
RI001-0125<\/td>\nRIBOAssure\u2122 RNase Inhibitor 5,000U<\/td>\n125 rxns
\n5,000U\/125\u03bcl<\/td>\n<\/tr>\n
QPR01-0100<\/td>\nPanProbes\u2122 One-Step RT-qPCR Kit<\/td>\n100 reactions
\n(20 \u03bcl vol)<\/td>\n<\/tr>\n
QPD01-0100<\/td>\nPanProbes\u2122 Universal qPCR MasterMix<\/td>\n100 reactions
\n(20 \u03bcl vol)<\/td>\n<\/tr>\n
QSR01-0100<\/td>\nPanGreen\u2122 One-Step RT-qPCR Kit<\/td>\n100 reactions
\n(20 \u03bcl vol)<\/td>\n<\/tr>\n
QSD01-0100<\/td>\nPanGreen\u2122 Universal SYBR\u00ae Green Mastermix<\/td>\n100 reactions
\n(20 \u03bcl vol)<\/td>\n<\/tr>\n
PDP01-0100<\/td>\nPlasmid miniPREP Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDP02-0020<\/td>\nPlasmid midiPREP Kit<\/td>\n20 rxns<\/td>\n<\/tr>\n
PDP03-0010<\/td>\nPlasmid maxiPREP Kit<\/td>\n10 rxns<\/td>\n<\/tr>\n
PDC01-0100<\/td>\nPCR Clean-Up & Gel Extraction Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC02-0100<\/td>\nGenomic DNA isolation dual kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC05-0100<\/td>\nDual Genomic DNA Isolation Kit (Plant)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC06-0100<\/td>\nDual Genomic DNA Isolation Kit(Tissue)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC09-0100<\/td>\nGenomic DNA Isolation Kit (Blood\/Cultured Cell\/Fungus)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC10-0100<\/td>\nGenomic DNA Isolation Kit (Plant)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC11-0100<\/td>\nGenomic DNA Isolation Kit (Tissue)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC12-0100<\/td>\nGenomic DNA Isolation Kit (Paraffin-embedded tissue)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDM01-0100<\/td>\nMbead Buffy Coat Genomic DNA Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDM02-0100<\/td>\nMbead Tissue Genomic DNA Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDM03-0100<\/td>\nMbead Bacteria Genomic DNA Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDM05-0100<\/td>\nMbead Plant Genomic DNA Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDM06-0100<\/td>\nMbead Blood\/Cell Genomic DNA Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDR01-0100<\/td>\nGenomic DNA Isolation Reagent<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDR02-0100<\/td>\nPlant Genomic DNA Isolation Reagent<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDR05-0100<\/td>\nGenomic DNA Isolation Reagent Kit(Blood\/Cultured Cell\/Tissue)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC04-0100<\/td>\nTotal RNA Isolation Kit (Blood\/Cultured Cell\/Fungus)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC07-0100<\/td>\nTotal RNA Isolation Kit (Plant)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC08-0100<\/td>\nTotal RNA Isolation Kit(Tissue)<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDR03-0100<\/td>\nTotal RNA Isolation Reagent<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDR04-0100<\/td>\nPlant Total RNA Isolation Reagent<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDC03-0100<\/td>\nVirus Nucleic Acid Isolation Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
PDM04-0100<\/td>\nMbead Virus Genomic Nucleic Acid Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
QP019M-0050<\/td>\nCOVID-19 Mutation RT-qPCR Detection Kit (Alpha, Delta, Kappa)<\/td>\n50 rxns<\/td>\n<\/tr>\n
QP019T-0100<\/td>\nCOVID-19 RT-qPCR Detection Kit Plus (TFDA)<\/td>\n100 rxns<\/td>\n<\/tr>\n
QP019-0100<\/td>\nSARS-CoV-2\/COVID-19 RT-qPCR Detection Kit (RUO)<\/td>\n100 rxns<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

RScript cDNA \u5408\u6210\u8a66\u5291\u76d2(200 U\/\u03bcl)\u00a0 | RScript cDNA Synthesis […]<\/p>\n","protected":false},"author":3,"featured_media":41102,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5372],"tags":[5575],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/41101"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=41101"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/41101\/revisions"}],"predecessor-version":[{"id":43305,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/41101\/revisions\/43305"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/41102"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=41101"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=41101"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=41101"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}