{"id":40800,"date":"2023-04-05T19:26:17","date_gmt":"2023-04-05T11:26:17","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=40800"},"modified":"2023-04-21T10:44:45","modified_gmt":"2023-04-21T02:44:45","slug":"anti-melanin-8c3-rabbit-igg-kappa-%e8%b2%a8%e8%99%9fab02814-23-0","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=40800","title":{"rendered":"Anti-Melanin [8C3], Rabbit IgG, Kappa \u8ca8\u865fAb02814-23.0"},"content":{"rendered":"

Anti-Melanin [8C3], Rabbit IgG, Kappa \u8ca8\u865fAb02814-23.0<\/a><\/span><\/h4>\n

Anti-Melanin [8C3]\u7531Protein A\u89aa\u548c\u7d14\u5316(\u6fc3\u5ea61mg\/ml)\uff0c\u514d\u75ab\u539f\uff1a\u6297\u9ad4\u662f\u7528\u9ed1\u8272\u7d20\u514d\u75ab\u96cc\u6027BALB\/c\u5c0f\u9f20 (\u9ed1\u8272\u7d20\u9846\u7c92\u7531 Paracoccidioides brasiliensis L-DOPA \u8a98\u5c0e\u7684\u9175\u6bcd\u7d30\u80de\u63d0\u53d6, \u61f8\u6d6e\u5728 1:1 \u4e2d(vol\/vol) \u4e0d\u5b8c\u5168\u5f17\u6c0f\u4f50\u5291\u7684\u4e73\u6db2)\u7279\u7570\u6027\uff1a\u8a72\u6297\u9ad4\u7d50\u5408\u9ed1\u8272\u7d20\u3002\u9ed1\u8272\u7d20\u662f\u4e00\u7a2e\u5b58\u5728\u65bc\u5927\u591a\u6578\u751f\u7269\u9ad4\u5167\u7684\u5929\u7136\u8272\u7d20\u5728\u7a31\u70ba\u9ed1\u7d20\u7d30\u80de\u7684\u7279\u6b8a\u7d30\u80de\u7fa4\u4e2d\u7522\u751f\u3002<\/p>\n

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Supplied in:<\/strong>\u00a0PBS with 0.02% Proclin 300.<\/p>\n<\/div>\n

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Storage Recommendation:<\/strong>\u00a0Store at 4\u2070C for up to 3 months. For longer storage, aliquot and store at -20\u2070C.<\/p>\n<\/div>\n

Alternative Name(s) of Target:<\/strong>\u00a0<\/span>CHEBI:89634<\/span><\/h4>\n

Immunogen:<\/strong><\/span>\u00a0<\/strong><\/span><\/h4>\n

The original antibody was generated by immunizing female BALB\/c mice with melanin particles extracted from Paracoccidioides brasiliensis L-DOPA-induced yeast cells suspended in a 1:1 (vol\/vol) emulsion of incomplete Freund adjuvant.<\/p>\n

Specificity:<\/strong><\/span><\/h4>\n

This antibody binds melanin. Melanin is a natural pigment found in most organisms is produced in a specialized group of cells called the melanocytes.<\/p>\n

Application Notes:<\/strong>\u00a0<\/span><\/h4>\n

Paracoccidioides brasiliensis is the agent of the human systemic disease paracoccidioidomycosis. The binding characterization of this antibody towards melanin from various organisms was done using ELISA. This antibody was also used in the immunofluorescence analysis of P. brasiliensis melanized yeast cells (with l-DOPA induction) and wild-type conidia (PMID: 21813659). 213Bismuth and 177Lutetium labelled humanized 8C3 antibody was used in the radioimmunotherapy (RIT) of experimental B16-F10 melanoma (PMID: 31323785). It was demonstrated that a combination of humanized anti-melanin antibody conjugated to 213Bismuth and anti-PD-1 ICB reduced tumor growth and increased survival in the Cloudman S91 murine melanoma DBA\/2 mouse model (PMID: 31991626). Male Cloudman S91-bearing DBA\/2 mice when treated intraperitoneally with a combination of anti-PD-1 alone or low-dose humanized anti-melanin antibody conjugated to177Lutetium RIT alone resulted in modest tumor reduction, while their combination significantly reduced tumor growth and increased survival, suggesting synergy (PMID: 33218169).<\/p>\n

Antibody first published in:<\/strong>\u00a0<\/span><\/h4>\n

Ur\u00e1n et al. Detection of antibodies against Paracoccidioides brasiliensis melanin in in vitro and in vivo studies during infection Clin Vaccine Immunol. 2011 Oct;18(10):1680-8.\u00a0PMID:21813659<\/a><\/p>\n

\"\"<\/p>\n

Absolute Antibody \u6210\u7acb\u65bc 2012 \u5e74\uff0c\u5176\u9858\u666f\u662f\u8b93\u6240\u6709\u4eba\u90fd\u80fd\u7372\u5f97\u5de5\u7a0b\u91cd\u7d44\u6297\u9ad4(engineered recombinant antibodies)\uff0c\u6211\u5011\u4ecd\u7136\u662f\u552f\u4e00\u4e00\u5bb6 100% \u5c08\u6ce8\u65bc\u91cd\u7d44\u6297\u9ad4\u6280\u8853(recombinant antibody technology)\u7684\u516c\u53f8\u3002\u5118\u7ba1\u91cd\u7d44\u6297\u9ad4(recombinant antibodies)\u662f\u88fd\u85e5\u884c\u696d(pharmaceutical industry)\u7684\u6a19\u6e96\uff0c\u4f46\u9019\u4e9b\u6297\u9ad4\u4e26\u672a\u5ee3\u6cdb\u7528\u65bc\u8a3a\u65b7\u6216\u7814\u7a76\u3002\u7136\u800c\uff0c\u7531\u65bc\u91cd\u7d44\u6297\u9ad4\u6bd4\u50b3\u7d71\u7684\u96dc\u4ea4\u7624\u7522\u751f\u7684\u55ae\u514b\u9686\u6297\u9ad4(hybridoma-produced monoclonal antibodies)\u5177\u6709\u8a31\u591a\u512a\u52e2\u3002Absolute Antibody \u4e00\u76f4\u8655\u65bc\u4f7f\u6240\u6709\u4eba\u66f4\u5bb9\u6613\u7372\u5f97\u91cd\u7d44\u6297\u9ad4\u7684\u904b\u52d5\u7684\u6700\u524d\u6cbf\u3002 2015 \u5e74\uff0c\u5728\u4e00\u7bc7\u95dc\u65bc\u6a19\u6e96\u5316\u7814\u7a76\u6297\u9ad4\u7684\u5fc5\u8981\u6027\u7684 Nature \u8ad6\u6587\u4e2d\u5f97\u5230\u8a8d\u53ef\uff0c2018 \u5e74\uff0cAbsolute Antibody\u8207co-authored\u8457\u4e86\u4e00\u7bc7 mAbs Journal \u8ad6\u6587\uff0c\u9032\u4e00\u6b65\u8aaa\u660e\u4e86\u4f7f\u7528\u91cd\u7d44\u6297\u9ad4\u7684\u91cd\u8981\u6027\u3002\u591a\u5e74\u4f86\uff0c\u96a8\u8457\u5c0d\u91cd\u7d44\u6297\u9ad4\u7684\u9700\u6c42\u589e\u52a0\uff0cAbsolute Antibody\u8fc5\u901f\u6210\u9577\u3002<\/p>\n

\u6211\u5011\u5c08\u6ce8\u65bc\u6297\u9ad4\u6e2c\u5e8f(antibody sequencing)\u3001\u5de5\u7a0b(engineering)\u548c\u91cd\u7d44\u8868\u9054(recombinant expression)\uff0c\u63d0\u4f9broyalty-free custom services\u5b9a\u5236\u670d\u52d9\u548c\u7368\u7279\u7684\u5de5\u7a0b\u91cd\u7d44\u6297\u9ad4\u548c Fc \u878d\u5408\u86cb\u767d\u8a66\u5291(c Fusion proteins)\u76ee\u9304\u3002Absolute Antibody\u7684\u5ba2\u6236\u5305\u62ec\u5168\u7403\u9818\u5148\u7684\u88fd\u85e5\u3001\u751f\u7269\u6280\u8853\u548c\u8a3a\u65b7\u516c\u53f8\uff0c\u4ee5\u53ca\u5168\u7403\u7684\u5b78\u8853\u7814\u7a76\u4eba\u54e1\uff1bAbsolute Antibody\u5728 65 \u500b\u4e0d\u540c\u7684\u570b\u5bb6\u64c1\u6709\u5ba2\u6236\uff0c\u4e26\u5df2\u5411\u6536\u5165\u6392\u540d\u524d 15 \u4f4d\u7684\u88fd\u85e5\u516c\u53f8\u4e2d\u7684 14 \u5bb6\u92b7\u552e\u7522\u54c1\u3002Absolute Antibody\u5728\u82f1\u683c\u862d\u6771\u5317\u90e8\u7372\u5f97 ISO 9001:2015 \u8a8d\u8b49\u7684\u88fd\u9020\u5de5\u5ee0\u4ee5\u53ca\u5728\u7f8e\u570b\u6ce2\u58eb\u9813\u548c\u8377\u862d\u963f\u59c6\u65af\u7279\u4e39\u7684\u5176\u4ed6\u8fa6\u4e8b\u8655\u70ba\u9019\u4e00\u570b\u969b\u5ba2\u6236\u7fa4\u63d0\u4f9b\u670d\u52d9\u3002<\/p>\n

Absolute Antibody \u5718\u968a\u5728\u6cbb\u7642\u6027\u6297\u9ad4(therapeutic antibody)\u548c\u91cd\u7d44\u6280\u8853recombinant technology\u9818\u57df\u64c1\u6709\u8c50\u5bcc\u7684\u7d93\u9a57\u3002Absolute Antibody\u5171\u540c\u64c1\u6709\u63d0\u4f9b\u9ad8\u8cea\u91cf\u7522\u54c1\u548c\u670d\u52d9\u7684\u77e5\u8b58\u548c\u5c08\u696d\u77e5\u8b58\uff0c\u4e26\u70ba\u5ba2\u6236\u7684\u6297\u9ad4\u958b\u767c\u9805\u76ee\u63d0\u4f9b\u9ad8\u6c34\u5e73\u7684\u8aee\u8a62\u3002\u81ea\u6210\u7acb\u4ee5\u4f86\uff0cAbsolute Antibody\u5df2\u70ba\u5ba2\u6236\u751f\u7522\u4e86 140 \u591a\u7a2e\u4e0d\u540c\u7684\u6297\u9ad4\u5f62\u5f0f\uff0c\u4e26\u958b\u767c\u4e86\u5305\u542b 11,000 \u591a\u7a2e\u5de5\u7a0b\u91cd\u7d44\u6297\u9ad4\u7684\u8a66\u5291\u76ee\u9304\u3002\u4f8b\u5982\uff0c\u67e5\u770b\u4e00\u500b\u76ee\u9304\u514b\u9686\uff08\u6297 EGFR \u6216\u897f\u59a5\u6614\u55ae\u6297\uff09\uff0c\u6709 30 \u591a\u7a2e\u4e0d\u540c\u7684\u73fe\u6210\u683c\u5f0f\u3002<\/p>\n

\u50c5 2021 \u5e74\uff0cAbsolute Antibody\u5c31\u5c0d 1200 \u591a\u500b\u96dc\u4ea4\u7624\u9032\u884c\u4e86\u6e2c\u5e8f(sequenced)\uff0c\u6210\u529f\u5b8c\u6210\u4e86 30 \u500b\u6297\u9ad4\u4eba\u6e90\u5316(antibody humanization projects)\u9805\u76ee\uff0c\u751f\u7522\u4e86 3500 \u591a\u7a2e\u4e0d\u540c\u7684\u6297\u9ad4\uff0c\u7e3d\u5171\u751f\u7522\u4e86 160 \u514b\u86cb\u767d\u8cea\u3002 2020 \u5e74\uff0cAbsolute Antibody\u6191\u85c9\u5feb\u901f\u7684\u570b\u969b\u64f4\u5f35\u7372\u5f97\u4e86\u570b\u969b\u8cbf\u6613\u4f01\u696d\u5973\u738b\u734e(Queen\u2019s Award)\u3002\u6211\u5011\u671f\u5f85\u7e7c\u7e8c\u9019\u7a2e\u589e\u9577\uff0c\u4e26\u671d\u8457\u6211\u5011\u70ba\u6240\u6709\u4eba\u8a2d\u8a08\u7684\u91cd\u7d44\u6297\u9ad4\u7684\u9858\u666f\u800c\u52aa\u529b\u200b\u200b\u3002<\/p>\n

\"\"<\/p>\n

\"\u7522\u54c1\u7279\u9ede2\"<\/p>\n

Fc Silent\u2122 \u6297\u9ad4<\/p>\n

Fc Silent\u2122 \u4ee3\u8868 Absolute Antibody \u958b\u767c\u7684\u57fa\u56e0\u5de5\u7a0b Fc \u57df\uff0c\u7528\u65bc\u7814\u7a76\u548c\u6aa2\u6e2c\u958b\u767c\u3002\u5b83\u5305\u542b\u6d88\u9664 Fc \u53d7\u9ad4 (Fc\u03b3R\u3001FcR) \u7d50\u5408\u7684\u95dc\u9375\u9ede\u7a81\u8b8a\uff0c\u6d88\u9664\u6297\u9ad4\u5b9a\u5411\u7d30\u80de\u6bd2\u6027 (ADCC) \u6548\u61c9\u5668\u529f\u80fd\u3002Fc Silent\u2122 \u6297\u9ad4\u4f7f\u7814\u7a76\u4eba\u54e1\u80fd\u5920\u5728\u9ad4\u5167\u53bb\u9664\u6548\u61c9\u5668\u529f\u80fd\u4e26\u6e1b\u5c11\u67d3\u8272\u65b9\u6cd5\u4e2d\u7684\u975e\u7279\u7570\u6027\u80cc\u666f\u3002\u5728\u9ad4\u5167\uff0c\u6297\u9ad4\u53d7\u76ca\u65bc\u8207 Fc \u7d50\u69cb\u57df\u76f8\u95dc\u7684\u9577\u8840\u6f3f\u534a\u8870\u671f (t1\/2)\uff0c\u800c\u6c92\u6709\u8207\u91ce\u751f\u578b Fc \u7d50\u69cb\u57df\u76f8\u95dc\u7684\u6eb6\u7d30\u80de\u514d\u75ab\u6548\u61c9\u6a5f\u5236\u3002\u5728\u9ad4\u5916\uff0c\u6297\u9ad4\u53ef\u7528\u65bc\u6d41\u5f0f\u7d30\u80de\u8853\u548c\u514d\u75ab\u7d44\u7e54\u5316\u5b78 (IHC) \u61c9\u7528\uff0c\u4ee5\u6e1b\u5c11\u7531 Fc \u53d7\u9ad4\u7d50\u5408\u7522\u751f\u7684\u975e\u7279\u7570\u6027\u80cc\u666f\u4fe1\u865f\u3002 Fc Silent\u2122 \u7a81\u8b8a\u4e0d\u5f71\u97ff\u6297\u9ad4\u7684\u5176\u4ed6\u7279\u6027\uff0c\u5305\u62ec\u5b83\u5011\u8207\u4e8c\u6297\u7684\u76f8\u5bb9\u6027\u3002\u6240\u6709 Absolute Antibody \u6297\u9ad4\u548c\u878d\u5408\u86cb\u767d\u5747\u5e36\u6709 Fc Silent\u2122 \u7d50\u69cb\u57df\u3002\u8a66\u5291\u76ee\u9304\u5305\u62ec\u7279\u5b9a\u7269\u7a2e\u7684 Fc Silent\u2122 \u7d50\u69cb\u57df\uff0c\u4f8b\u5982\u4eba\u985e\u3001\u5c0f\u9f20\u3001\u5154\u3001\u5927\u9f20\u548c\u5009\u9f20<\/p>\n

\u5716 2 \u4f7f\u7528\u8868\u9762\u7b49\u96e2\u5b50\u5171\u632f (SPR) \u548c\u76f4\u63a5\u7af6\u722d\u6027\u6291\u5236 ELISA \u986f\u793a Fc Silent\u2122 \u5f62\u5f0f\u4e0d\u8207\u9f20\u6216\u4eba Fc \u53d7\u9ad4\u76f8\u4e92\u4f5c\u7528\u3002\u8a72\u6578\u64da\u7531 Dr.\u5357\u5b89\u666e\u9813\u5927\u5b78\u7684\u65af\u8482\u82ac\u00b7\u6bd4\u723e\u65af\u3002<\/p>\n

\u514d\u75ab\u7642\u6cd5\u7814\u7a76(Immunotherapy Research) \u6d3b\u9ad4\u7528\u91cd\u7d44\u6297\u9ad4<\/p>\n

\u91dd\u5c0d\u6cbb\u7642\u7814\u7a76\uff0c\u6297\u86cb\u767d\u8cea\u7684\u6297\u9ad4\u53c3\u8207co-stimulation\u548c\u514d\u75ab\u7cfb\u7d71\u8abf\u7bc0(immune cell regulation)\u3002 \u6709\u4e9b\u9032\u5165\u81e8\u5e8a\u968e\u6bb5(clinic)\uff1b\u66f4\u591a\u90e8\u4efd\u4ecd\u8655\u65bc\u7814\u7a76\u968e\u6bb5\u3002\u514d\u75ab\u7d30\u80de\u4fe1\u865f\u50b3\u5c0e(immune cell signaling)\u7684\u8a31\u591a\u65b9\u9762\u4ecd\u7136\u672a\u77e5\uff0cAbsolute Antibody\u958b\u767c\u4e86\u91dd\u5c0d\u81e8\u5e8a\u76f8\u95dc\u86cb\u767d\u7684\u91cd\u7d44\u5de5\u7a0b\u6297\u9ad4\uff0c\u63d0\u4f9b\u4e00\u7cfb\u5217\u514d\u75ab\u7642\u6cd5\u7814\u7a76(Immunotherapy Research) \u6d3b\u9ad4\u7528\u91cd\u7d44\u6297\u9ad4\u3002<\/p>\n