{"id":40788,"date":"2023-04-05T10:22:16","date_gmt":"2023-04-05T02:22:16","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=40788"},"modified":"2023-04-21T10:42:47","modified_gmt":"2023-04-21T02:42:47","slug":"dhvd3125-%e4%ba%8c%e7%be%a5%e5%9f%ba%e7%b6%ad%e7%94%9f%e7%b4%a0-d3-elisa-%e8%a9%a6%e5%8a%91%e7%9b%92-elk-biotechnology%e8%b2%a8%e8%99%9felk8091","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=40788","title":{"rendered":"DHVD3(1,25-\u4e8c\u7fa5\u57fa\u7dad\u751f\u7d20 D3) ELISA \u8a66\u5291\u76d2 |ELK Biotechnology\u8ca8\u865fELK8091"},"content":{"rendered":"

DHVD3(1,25-\u4e8c\u7fa5\u57fa\u7dad\u751f\u7d20 D3) ELISA \u8a66\u5291\u76d2 -ELK Biotechnology\u8ca8\u865fELK8091<\/a><\/span><\/h4>\n

DHVD3(1,25-Dihydroxyvitamin D3) ELISA Kit<\/p>\n

\u9748\u654f\u5ea6156.25-10000 pg\/mL\u3001\u6700\u4f4e\u9748\u654f\u5ea649.5 pg\/mL<\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39:<\/span><\/h5>\n

DHVD3(1,25-\u4e8c\u7fa5\u57fa\u7dad\u751f\u7d20 D3) ELISA \u8a66\u5291\u76d2 | ELK8091 (Sandwich ELISA)\uff0c\u5075\u6e2c\u7684\u9748\u654f\u5ea6156.25-10000 pg\/mL\u3001\u6700\u4f4e\u9748\u654f\u5ea649.5 pg\/mL\u3002ELISA\u539f\u7406\u70ba\u5229\u7528\u6297\u9ad4\u8207\u6297\u539f\u4e4b\u9593\u7684\u9ad8\u5ea6\u5c08\u4e00\u6027\uff0c\u505a\u70ba\u5b9a\u91cf\u6a19\u8a8c\u7684\u65b9\u6cd5\uff1b\u7121\u8ad6\u6e2c\u91cf\u6297\u539f\u6216\u6e2c\u91cf\u6297\u9ad4\u90fd\u6709\u5f88\u9ad8\u7684\u9748\u654f\u5ea6\u8ddf\u5c08\u4e00\u6027\u3002\u8a72\u6aa2\u6e2c\u65b9\u6cd5\u5c0dDHVD3(1,25-\u4e8c\u7fa5\u57fa\u7dad\u751f\u7d20 D3) \u5177\u6709\u9ad8\u9748\u654f\u5ea6\u548c\u51fa\u8272\u7684\u7279\u7570\u6027\u3002ELISA\u5546\u54c1\u7684\u578b\u5f0f\u591a\u5143\uff0c \u63d0\u4f9b\u4e09\u660e\u6cbbELISA\u3001\u7af6\u722d\u578bELISA\u3002\u53ef\u61c9\u7528\u65bcserum, plasma, tissue homogenates, cell lysates, cell culture\u3002\u4e26\u5728 450nm \u00b1 10nm \u7684\u6ce2\u9577\u4e0b\u7528\u5206\u5149\u5149\u5ea6\u8a08\u6e2c\u91cf\u984f\u8272\u8b8a\u5316\u3002\u7136\u5f8c\u901a\u904e\u5c07\u6a23\u54c1\u7684 OD \u8207\u6a19\u6e96\u66f2\u7dda\u9032\u884c\u6bd4\u8f03\u4f86\u78ba\u5b9a\u6a23\u54c1\u4e2dDHVD3(1,25-\u4e8c\u7fa5\u57fa\u7dad\u751f\u7d20 D3) \u7684\u6fc3\u5ea6\u3002<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product name:<\/td>\nDHVD3(1,25-Dihydroxyvitamin D3) ELISA Kit<\/td>\n<\/tr>\n
Reactivity:<\/td>\nGeneral<\/td>\n<\/tr>\n
Alternative Names:<\/td>\nDH-VD3; Calcitriol; Rocaltrol; Calcijex; 1,25-(OH)2D3; 1,25-Dihydroxycholecalciferol; 1,25-Dihydroxy Vitamin D3<\/td>\n<\/tr>\n
Assay Type:<\/td>\nCompetitive Inhibition<\/td>\n<\/tr>\n
Sensitivity:<\/td>\n49.5 pg\/mL<\/td>\n<\/tr>\n
Standard:<\/td>\n10000 pg\/mL<\/td>\n<\/tr>\n
Range:<\/td>\n156.25-10000 pg\/mL<\/td>\n<\/tr>\n
Sample Type:<\/td>\nserum, plasma and other biological fluids<\/td>\n<\/tr>\n
Assay Length:<\/td>\n2h<\/td>\n<\/tr>\n
Research Area:<\/td>\nMetabolic pathway;Bone metabolism;<\/td>\n<\/tr>\n
Test principle:<\/td>\nThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 1,25-Dihydroxyvitamin D3(DHVD3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 1,25-Dihydroxyvitamin D3(DHVD3). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm \u00b1 10nm. The concentration of 1,25-Dihydroxyvitamin D3(DHVD3) in the samples is then determined by comparing the OD of the samples to the standard curve.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\"<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716<\/p>\n

ELK<\/strong>\u539f\u5ee0\u7db2\u5740<\/strong><\/span>\u00a0 >><\/span>\u00a0http:\/\/www.elkbiotech.com\/<\/a>\u00a0<\/span><\/strong><\/span><\/p>\n

ELK Biotechnology\u516c\u53f8\u662f\u4e00\u5bb6\u5c08\u6ce8\u65bc\u751f\u547d\u79d1\u5b78\u3001\u514d\u75ab\u7814\u7a76\u7684\u9ad8\u79d1\u6280\u751f\u7269\u4f01\u696d\uff0c\u4f4d\u65bcBiolake, Donghu New & High Technology Development Zone, Wuhan city.ELK Biotechnology\u662f\u4e00\u5bb6\u5c08\u6ce8\u65bc\u514d\u75ab\u7522\u54c1\u548c\u5206\u5b50\u751f\u7269\u5b78\u7522\u54c1\u7814\u767c\u548c\u751f\u7522\u7684\u9ad8\u79d1\u6280\u751f\u7269\u516c\u53f8\u3002\u7522\u54c1\u6db5\u84cbELISA\u8a66\u5291\u76d2\u3001\u6297\u9ad4\u3001\u57fa\u56e0\u7d44\u63d0\u53d6\u8a66\u5291\u3001\u5206\u5b50\u751f\u7269\u5b78\u8a66\u5291\u3002\u6d89\u53ca\u514d\u75ab\u5316\u5b78\u3001\u816b\u7624\u7814\u7a76\u3001\u795e\u7d93\u751f\u7269\u5b78\u3001\u7d30\u80de\u9031\u671f\u3001\u4fe1\u865f\u8f49\u5c0e\u7b49\u9818\u57df\u3002\u6211\u5011\u7684\u7522\u54c1\u6709\u56b4\u683c\u7684\u7814\u767c\u548c\u8cea\u91cf\u6aa2\u6e2c\u6d41\u7a0b\u3002\u6bcf\u500b\u7522\u54c1\u90fd\u6709\u5b8c\u6574\u7684\u7814\u767c\u3001\u751f\u7522\u548c\u8cea\u6aa2\u8a18\u9304\u3002\u7d93\u9a57\u8c50\u5bcc\u7684\u7814\u767c\u4eba\u54e1\uff0c\u5b8c\u5584\u7684\u8cea\u6aa2\u652f\u6301\u90e8\u9580\uff0c\u56b4\u683c\u7684\u6aa2\u6e2c\u6d41\u7a0b\uff0c\u70ba\u5ba2\u6236\u63d0\u4f9b\u8cea\u91cf\u53ef\u9760\u7684\u79d1\u7814\u7522\u54c1\u3002<\/p>\n

\u200b<\/span>\"ELK1.jpg\"\u200b<\/span><\/span>\u200b<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

DHVD3(1,25-\u4e8c\u7fa5\u57fa\u7dad\u751f\u7d20 D3) ELISA \u8a66\u5291\u76d2 -ELK Biotechnology\u8ca8\u865fELK […]<\/p>\n","protected":false},"author":3,"featured_media":34915,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5524],"tags":[5529,5528],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40788"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=40788"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40788\/revisions"}],"predecessor-version":[{"id":41407,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40788\/revisions\/41407"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/34915"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=40788"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=40788"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=40788"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}