{"id":40756,"date":"2023-04-04T22:12:45","date_gmt":"2023-04-04T14:12:45","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=40756"},"modified":"2025-11-04T16:30:56","modified_gmt":"2025-11-04T08:30:56","slug":"cyto-id-%e8%87%aa%e5%99%ac%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-autophagy-detection-kit%e8%b2%a8%e8%99%9fenz-51031-k200","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=40756","title":{"rendered":"CYTO-ID \u00ae\u81ea\u566c\u6aa2\u6e2c\u8a66\u5291\u76d2 |  Autophagy detection kit\u8ca8\u865fENZ-51031-K200"},"content":{"rendered":"<h4><span style=\"font-size: 14pt;\"><strong><span style=\"color: #993366;\">\u81ea\u566c(Autophagy )\u6aa2\u6e2c |<\/span><span style=\"color: #ff9900;\"> Autophagy Cellular Analysis Kits\u00a0<\/span><\/strong><\/span><\/h4>\n<p><span style=\"color: #33cccc; font-size: 12pt;\"><a style=\"color: #33cccc;\" href=\"http:\/\/biopioneer.com.tw\/?p=40756\">CYTO-ID\u00a0<sup>\u00ae<\/sup>\u81ea\u566c\u6aa2\u6e2c\u8a66\u5291\u76d2 |\u00a0\u00a0Autophagy detection kit\u8ca8\u865f<b>ENZ-51031-K200<\/b><\/a><\/span><\/p>\n<p><span style=\"color: #999999;\"><b>\u4e00\u7a2e\u7528\u65bc\u76e3\u6e2c\u6d3b\u7d30\u80de\u81ea\u566c\u7684\u975e\u8f49\u67d3\u5b9a\u91cf\u5206\u6790<\/b><\/span><\/p>\n<p>\u81ea\u566c(Autophagy)\u662f\u4e00\u7a2e\u61c9\u6fc0\u8a98\u5c0e\u7684\u4fdd\u8b77\u6a5f\u5236(stress-induced protective mechanism)\u3002\u5728\u57fa\u790e\u689d\u4ef6\u4e0b\u4e0d\u592a\u6d3b\u8e8d\uff0c\u771f\u6838\u7d30\u80de\u5728\u53d7\u5230\u67d0\u4e9b\u4e0d\u5229\u689d\u4ef6\uff08\u5982\u71df\u990a\u8017\u76e1\u548c\u5316\u5b78\u6216\u74b0\u5883\u58d3\u529b\uff09\u6642\u901a\u904e\u6eb6\u9176\u9ad4\u4ecb\u5c0e(lysosome-mediated)\u7684\u7d30\u80de\u5167\u5bb9\u7269\u7684\u5927\u91cf\u964d\u89e3\u4f86\u5229\u7528\u8a72\u6a5f\u5236\u3002\u81ea\u566c\u6d3b\u6027(autophagic activity)\u589e\u52a0\u5728\u764c\u75c7(cancer)\u3001\u795e\u7d93\u8b8a\u6027(neurodegeneration)\u3001\u5fc3\u8840\u7ba1\u75be\u75c5(\u00a0cardiovascular disease)\u548c\u7cd6\u5c3f\u75c5(\u00a0diabetes)\u7684\u75c5\u7406\u5b78\u4e2d\u7684\u4f5c\u7528\u5df2\u5f97\u5230\u5ee3\u6cdb\u8a8d\u53ef\u548c\u666e\u904d\u7814\u7a76\u3002\u5982\u679c\u6eb6\u9176\u9ad4\u529f\u80fd(\u00a0lysosomal function)\u53d7\u5230\u6291\u5236\uff0c\u53ef\u4ee5\u901a\u904e\u81ea\u566c\u5c0f\u6ce1\u7684\u589e\u5f37\u7a4d\u7d2f\u4f86\u89c0\u5bdf\u81ea\u566c\u901a\u91cf\u7684\u8a98\u5c0e\uff0c\u5f9e\u800c\u963b\u6b62\u9019\u4e9b\u5c0f\u6ce1\u7684\u53bb\u9664\u3002<\/p>\n<p>Enzo Life Sciences CYTO-ID \u00ae\u81ea\u566c\u6aa2\u6e2c\u8a66\u5291\u76d2(CYTO-ID\u00ae Autophagy Detection Kit , ENZ-51031-K200)\u4f7f\u7528\u4e00\u7a2e\u9078\u64c7\u6027\u6a19\u8a18\u7a4d\u7d2f\u7684\u81ea\u566c\u6ce1\u7684\u65b0\u578b\u67d3\u6599\u6e2c\u91cf\u81ea\u566c\u6ce1(autophagic vacuoles)\u4e26\u76e3\u6e2c\u6eb6\u9176\u9ad4\u6291\u5236\u6d3b\u7d30\u80de\u4e2d\u7684\u81ea\u566c\u6d41(autophagic flux )\u3002488nm \u53ef\u6fc0\u767c\u7684\u7da0\u8272\u67d3\u6599(488nm-excitable green dye )\u5df2\u901a\u904e\u9451\u5b9a\u53ef\u6ef4\u5b9a\u529f\u80fd\u90e8\u5206\u9032\u884c\u4e86\u512a\u5316\uff0c\u9019\u4e9b\u90e8\u5206\u5141\u8a31\u5c0d\u6eb6\u9176\u9ad4\u7684\u67d3\u8272\u6700\u5c11\uff0c\u540c\u6642\u5728\u647b\u5165\u524d\u81ea\u566c\u9ad4(pre-autophagosomes)\u3001\u81ea\u566c\u9ad4(\u00a0autophagosomes)\u548c\u81ea\u566c\u6eb6\u9176\u9ad4\uff08\u81ea\u566c\u6eb6\u9176\u9ad4\uff09\u6642\u8868\u73fe\u51fa\u660e\u4eae\u7684\u87a2\u5149\u3002\u8a72\u8a66\u5291\u76d2\u9084\u5305\u62ec\u7528\u65bc\u6838\u67d3\u8272\u7684 Hoechst 33342 \u67d3\u6599\u3001\u81ea\u566c\u8a98\u5c0e\u5291-\u96f7\u5e15\u9ef4\u7d20(Rapamycin\uff09\u548c\u6eb6\u9176\u9ad4\u6291\u5236\u5291-\u6c2f\u55b9\uff08Chloroquine\uff09\u3002<\/p>\n<p><strong><span style=\"color: #800000; font-size: 12pt;\">\u4f5c\u7528\u6a5f\u5236 (Mechanism of Action &amp; Autophagy Pathway)<\/span><\/strong><\/p>\n<p>CYTO-ID\u00a0<sup>\u00ae<\/sup>\u81ea\u566c\u6aa2\u6e2c\u8a66\u5291\u76d2\u662f CELLESTIAL\u00a0<sup>\u00ae<\/sup>\u7522\u54c1\u7dda\u3001\u8a66\u5291\u548c\u6aa2\u6e2c\u8a66\u5291\u76d2\u7684\u6210\u54e1\uff0c\u5176\u4e2d\u5305\u542b\u87a2\u5149\u5206\u5b50\u63a2\u91dd\uff0c\u9019\u4e9b\u63a2\u91dd\u5df2\u91dd\u5c0d\u6d3b\u7d30\u80de\u5206\u6790\u61c9\u7528\u9032\u884c\u4e86\u5ee3\u6cdb\u7684\u57fa\u6e96\u6e2c\u8a66\u3002\u8a72\u63a2\u91dd\u662f\u4e00\u7a2e\u967d\u96e2\u5b50\u5169\u89aa\u6027\u793a\u8e2a\u5291 (CAT) \u67d3\u6599\uff0c\u5b83\u4ee5\u8207\u8a98\u5c0e\u78f7\u8102\u6c89\u7a4d\u75c7\u7684\u85e5\u7269\u985e\u4f3c\u7684\u65b9\u5f0f\u5feb\u901f\u5206\u914d\u5230\u7d30\u80de\u4e2d\u3002\u4ed4\u7d30\u9078\u64c7\u67d3\u6599\u4e0a\u7684\u53ef\u6ef4\u5b9a\u529f\u80fd\u90e8\u5206\u53ef\u9632\u6b62\u5176\u5728\u6eb6\u9176\u9ad4\u4e2d\u7a4d\u7d2f\uff0c\u4f46\u80fd\u5920\u6a19\u8a18\u8207\u81ea\u566c\u9014\u5f91\u76f8\u95dc\u7684\u6db2\u6ce1\u3002<\/p>\n<div class=\"productdescription\"><\/div>\n<div class=\"productdescription\"><img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/04\/AUTOPHAGY-CYTO-ID.jpg\" alt=\"\" \/><\/div>\n<div class=\"productdescription\"><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/04\/1-CYTO-ID-%C2%AE%E8%87%AA%E5%99%AC%E6%AA%A2%E6%B8%AC%E8%A9%A6%E5%8A%91%E7%9B%92-Autophagy-detection-kit%E8%B2%A8%E8%99%9FENZ-51031-K200.jpg\" alt=\"\" width=\"656\" height=\"352\" \/><\/div>\n<ul>\n<li>\u5feb\u901f\uff0c\u7121\u9700\u8f49\u67d3<\/li>\n<li>\u4f7f\u7528\u5df2\u77e5\u7684\u81ea\u566c\u6d3b\u6027\u6291\u5236\u5291\u548c\u6fc0\u6d3b\u5291\u9a57\u8b49\u7684\u65b9\u6848<\/li>\n<li>\u9078\u64c7\u6027\u548c\u5168\u9762\u7684\u67d3\u8272\u53ef\u4ee5\u5340\u5206\u81ea\u566c\u901a\u91cf\u548c\u81ea\u566c\u6eb6\u9176\u9ad4\u7a4d\u7d2f<\/li>\n<li>\u6eb6\u9176\u9ad4\u67d3\u8272\u53ef\u5ffd\u7565\u4e0d\u8a08\uff0c\u964d\u4f4e\u4e86\u5176\u4ed6\u67d3\u6599\u6240\u898b\u7684\u80cc\u666f<\/li>\n<li>\u4fc3\u9032\u81ea\u566c\u6fc0\u6d3b\u5291\u548c\u6291\u88fd\u5291\u7684\u9ad8\u901a\u91cf\u7be9\u9078<\/li>\n<\/ul>\n<p><span style=\"color: #800000; font-size: 12pt;\">\u5feb\u901f\uff0c\u7121\u9700\u8f49\u67d3 (Profile Autophagy Without Transfection)<\/span><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/04\/2.-CYTO-ID-%C2%AE%E8%87%AA%E5%99%AC%E6%AA%A2%E6%B8%AC%E8%A9%A6%E5%8A%91%E7%9B%92-Autophagy-detection-kit%E8%B2%A8%E8%99%9FENZ-51031-K200.jpg\" alt=\"\" width=\"742\" height=\"248\" \/><\/p>\n<p><span style=\"color: #800000;\">Visualization of Autophagic Accumulation and Autophagic Flux\u00a0<\/span><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/04\/3.-CYTO-ID-%C2%AE%E8%87%AA%E5%99%AC%E6%AA%A2%E6%B8%AC%E8%A9%A6%E5%8A%91%E7%9B%92-Autophagy-detection-kit%E8%B2%A8%E8%99%9FENZ-51031-K200.jpg\" alt=\"\" width=\"734\" height=\"235\" \/><\/p>\n<p><img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/04\/3-CYTO-ID-%C2%AE%E8%87%AA%E5%99%AC%E6%AA%A2%E6%B8%AC%E8%A9%A6%E5%8A%91%E7%9B%92-Autophagy-detection-kit%E8%B2%A8%E8%99%9FENZ-51031-K200.gif\" alt=\"\" \/><\/p>\n<p>\u7121\u9700\u8f49\u67d3\u5373\u53ef\u5c0d\u81ea\u566c\u6ce1\u9032\u884c\u7701\u6642\u3001\u5feb\u901f\u548c\u5168\u9762\u7684\u6a19\u8a18\u3002\u70ba\u4e86\u5c55\u793a CYTO-ID\u00a0<sup>\u00ae<\/sup>\u00a0Green \u6aa2\u6e2c\u8a66\u5291\u7684\u512a\u52e2\uff0c\u9996\u5148\u7528 RFP-LC3 \u8868\u9054\u8f09\u9ad4\u8f49\u67d3 HeLa \u7d30\u80de\uff0c\u7528 10 \u00b5M Tamoxifen \u8655\u7406\u904e\u591c\uff0c\u7136\u5f8c\u7528 CYTO-ID\u00a0<sup>\u00ae<\/sup>\u00a0Green \u6aa2\u6e2c\u8a66\u5291\u67d3\u8272\u3002\u8207\u57fa\u65bc\u904e\u591c\u8f49\u67d3\u7684\u6e2c\u5b9a\u4e0d\u540c\uff0cCYTO-ID\u00a0<sup>\u00ae<\/sup>\u00a0Green \u6aa2\u6e2c\u8a66\u5291\u65b9\u6cd5\u53ef\u5728 15-30 \u5206\u9418\u5167\u6a19\u8a18 100% \u7684\u7d30\u80de\u3002\u5716 A\uff1a\u7da0\u8272\u4fe1\u865f\u8868\u793a\u81ea\u566c\u5c0f\u6ce1\u7684 CYTO-ID\u00a0<sup>\u00ae<\/sup>\u7da0\u8272\u67d3\u8272\uff1b\u5716 B\uff1aRFP-LC3 \u5728\u6210\u529f\u8f49\u67d3\u7684\u7d30\u80de\u5b50\u96c6\u4e2d\u7684\u8868\u9054\uff08\u7d05\u8272\uff09\uff1b\u9762\u677f C\uff1a\u5408\u6210\u5716\u50cf\uff0c\u986f\u793a CYTO-ID\u00a0<sup>\u00ae<\/sup>\u7da0\u8272\u67d3\u6599\u6a19\u8a18\u7684\u56ca\u6ce1\u8207\u81ea\u566c\u9ad4\u7684\u7279\u7570\u6027\u6a19\u8a18\u7269 LC3 \u5171\u5b9a\u4f4d\u3002<\/p>\n<p><img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/04\/CYTO-ID-%C2%AE%E8%87%AA%E5%99%AC%E6%AA%A2%E6%B8%AC%E8%A9%A6%E5%8A%91%E7%9B%92-Autophagy-detection-kit%E8%B2%A8%E8%99%9FENZ-51031-K200.gif\" alt=\"\" \/><\/p>\n<p><a href=\"https:\/\/www.enzolifesciences.com\/ENZ-51031\/cyto-id-autophagy-detection-kit\/\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/03\/%E5%BC%95%E7%94%A8%E6%96%87%E7%8D%BB_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1-300x120.jpg\" alt=\"\u5f15\u7528\u6587\u737b_\u5de5\u4f5c\u5340\u57df 1\" width=\"146\" height=\"59\" \/><\/a><\/p>\n<p>Product Literature References<br \/>\n3,4,5-O-tricaffeoylquinic acid with anti-radiation activity suppresses LPS-induced NLRP3 inflammasome activation via autophagy in THP-1 macrophages: J. Liu, et al.; Mol. Immunol. 147, 187 (2022), Abstract;<br \/>\nA peptide interfering with the dimerization of oncogenic KITENIN protein and its stability suppresses colorectal tumour progression: S.J. Kim et al.; Clin. Transl. Med. 12, e871 (2022), Abstract;<br \/>\nActivated amino acid response pathway generates apatinib resistance by reprograming glutamine metabolism in non-small-cell lung cancer: X. Zhou, et al.; Cell Death Dis. 13, 636 (2022), Abstract;<br \/>\nAir Plasma-Activated Medium Evokes a Death-Associated Perinuclear Mitochondrial Clustering: M. Suzuki-Karasaki, et al.; Int. J. Mol. Sci. 23, 1124 (2022), Abstract;<br \/>\nAlcohol Metabolism Enriches Squamous Cell Carcinoma Cancer Stem Cells That Survive Oxidative Stress via Autophagy: M. Shimonosono, et al.; Biomolecules 11, 1479 (2022), Abstract;<br \/>\nAsiatic acid from Cyclocarya paliurus regulates the autophagy-lysosome system via directly inhibiting TGF-\u03b2 type I receptor and ameliorates diabetic nephropathy fibrosis: X.X. Zhang, et al.; Food Funct. 13, 5536 (2022), Abstract;<br \/>\nAutophagy induced by taurolidine protects against polymicrobial sepsis by promoting both host resistance and disease tolerance: J. Huang, et al.; PNAS 119, e2121244119 (2022), Abstract;<br \/>\nBCL2 inhibitor ABT-199 and BCL2L1 inhibitor WEHI-539 coordinately promote NOXA-mediated degradation of MCL1 in human leukemia cells: J.T. Chiou, et al.; Chem. Biol. Interact. 361, 109978 (2022), Abstract;<br \/>\nCelastrol upregulated ATG7 triggers autophagy via targeting Nur77 in colorectal cancer: W. Zhang, et al.; Phytomedicine 104, 154280 (2022), Abstract;<br \/>\nCellular Effects of Cyclodextrins: Studies on HeLa Cells: \u00c1. Ruszny\u00e1k, et al.; Molecules 27, 1589 (2022), Abstract;<br \/>\nCNS serotonin content mediating food deprivation-enhanced learning is regulated by hemolymph tryptophan concentration and autophagic flux in the pond snail: Y. Totani, et al.; Nutr. Neurosci. (2022), Abstract;<br \/>\nCombined Levo-tetrahydropalmatine and diphenyleneiodonium chloride enhances antitumor activity in hepatocellular carcinoma: X. Yin, et al.; Pharmacol. Res. 179, 106219 (2022), Abstract;<br \/>\nGallium(III) Complex with Cloxyquin Ligands Induces Ferroptosis in Cancer Cells and Is a Potent Agent against Both Differentiated and Tumorigenic Cancer Stem Rhabdomyosarcoma Cells: M. Hreusova, et al.; Bioinorg. Chem. Appl. 2022, 3095749 (2022), Abstract;<br \/>\nHIF-1\u03b1\/FOXO1 axis regulated autophagy is protective for \u03b2 cell survival under hypoxia in human islets: R. Liang, et al.; Biochim. Biophys. Acta Mol. Basis Dis. 1868, 166356 (2022), Abstract;<br \/>\nIdentification of distinct slow mode of reversible adaptation of pancreatic ductal adenocarcinoma to the prolonged acidic pH microenvironment: T.C. Wu, et al.; J. Exp. Clin. Cancer Res. 41, 137 (2022), Abstract;<br \/>\nInhibition of B-cell lymphoma 2 family proteins alters optical redox ratio, mitochondrial polarization, and cell energetics independent of cell state: A.A. Gillette, et al.; J. Biomed. Opt. 27, 056505 (2022), Abstract;<br \/>\nIntegrative analysis of the miRNA-mRNA regulation network in hemocytes of Penaeus vannamei following Vibrio alginolyticus infection: F. Wang, et al.; Dev. Comp. Immunol. 131, 104390 (2022), Abstract;<br \/>\nMagnesium Homeostasis in Myogenic Differentiation &#8211; A Focus on the Regulation of TRPM7, MagT1 and SLC41A1 Transporters: M. Zocchi, et al.; Int. J. Mol. Sci. 23, 1658 (2022), Abstract;<br \/>\nmiR-23b-3p Modulating Cytoprotective Autophagy and Glutamine Addiction in Sorafenib Resistant HepG2, a Hepatocellular Carcinoma Cell Line: R. Kaur, et al.; Genes 13, 1375 (2022), Abstract;<br \/>\nNanoparticle formulation of the fusion protein virus like particles of respiratory syncytial virus stimulates enhanced in vitro antigen presentation and autophagy: I. Menon, et al.; Int. J. Pharm. 623, 121919 (2022), Abstract;<br \/>\nNEDD4L binds the proteasome and promotes autophagy and bortezomib sensitivity in multiple myeloma: X. Huang, et al.; Cell Death Dis. 13, 197 (2022), Abstract;<br \/>\nNigrosporins B, a Potential Anti-Cervical Cancer Agent, Induces Apoptosis and Protective Autophagy in Human Cervical Cancer Ca Ski Cells Mediated by PI3K\/AKT\/mTOR Signaling Pathway: J. Zhang, et al.; Molecules 27, 2431 (2022), Abstract;<br \/>\nPHF20 is crucial for epigenetic control of starvation-induced autophagy through enhancer activation: S.W. Park, et al.; Nucleic Acids Res. 50, 7856 (2022), Abstract;<br \/>\nProtective autophagy decreases lorlatinib cytotoxicity through Foxo3a-dependent inhibition of apoptosis in NSCLC: C. Lu, et al.; Cell Death Discov. 8, 221 (2022), Abstract;<br \/>\nRegulatory Effects of Astragaloside IV on Hyperglycemia-Induced Mitophagy in Schwann Cells: X. Wei, et al.; Evid. Based Complement. Alternat. Med. 2022, 7864308 (2022), Abstract;<br \/>\nSiah-1-interacting protein regulates mutated huntingtin protein aggregation in Huntington\u2019s disease models: E. Latoszek, et al.; Cell Biosci. 12, 34 (2022), Abstract;<br \/>\nSpautin-1 inhibits mitochondrial complex I and leads to suppression of the unfolded protein response and cell survival during glucose starvation: K. Kunimasa, et al.; Sci. Rep. 12, 11533 (2022), Abstract;<br \/>\nThe preventive effect of loganin on oxidative stress-induced cellular damage in human keratinocyte HaCaT cells: C. Park, et al.; Biosci. 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