{"id":40614,"date":"2023-03-28T13:57:59","date_gmt":"2023-03-28T05:57:59","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=40614"},"modified":"2023-03-28T14:20:48","modified_gmt":"2023-03-28T06:20:48","slug":"%e7%b4%b0%e8%83%9e%e8%89%b2%e7%b4%a0c%ef%bc%88%e4%ba%ba%ef%bc%89elisa%e8%a9%a6%e5%8a%91%e7%9b%92-abcam%e8%b2%a8%e8%99%9f-ab221832-e4286-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=40614","title":{"rendered":"\u7d30\u80de\u8272\u7d20c\uff08\u4eba\uff09ELISA\u8a66\u5291\u76d2 | ABCAM\u8ca8\u865f ab221832 (E4286-100)"},"content":{"rendered":"

\u7d30\u80de\u8272\u7d20c\uff08\u4eba\uff09ELISA\u8a66\u5291\u76d2\u00a0<\/em><\/strong>\u00a0<\/strong>|\u00a0<\/strong>\u8ca8\u865f\u00a0ABCAM\u8ca8\u865f ab221832 (E4286-100)<\/strong><\/a><\/span><\/p>\n

Cytochrome c (Human) ELISA Kit\u00a0<\/em><\/strong>An Sandwich ELISA kit for the quantitative measurement of human Cytochrome c in human serum, plasma, culture supernatants and other biological fluids.<\/em><\/strong><\/p>\n

\"\"<\/p>\n

\u4eba\u7d30\u80de\u8272\u7d20 C ELISA \u8a66\u5291\u76d2 (ab221832)<\/strong><\/span><\/div>\n
\n

SimpleStep ELISA \u6280\u8853\u53ef\u4e00\u6b65\u5f62\u6210\u6297\u9ad4-\u6297\u539f\u8907\u5408\u7269\uff0c\u5c07\u6aa2\u6e2c\u6642\u9593\u7e2e\u77ed\u81f3 90 \u5206\u9418\u3002\u4e00\u6b21\u5c07\u6a23\u54c1\u6216\u6a19\u6e96\u54c1\u548c\u6297\u9ad4\u6df7\u5408\u7269\u5168\u90e8\u6dfb\u52a0\u5230\u5b54\u4e2d\uff0c\u5b75\u80b2\u3001\u6e05\u6d17\u4e26\u6dfb\u52a0\u6700\u7d42\u5e95\u7269\u3002\u6709\u95dc\u8a73\u7d30\u7684\u5206\u6b65\u6307\u5357\uff0c\u8acb\u53c3\u95b1\u5354\u8b70\u3002<\/p>\n<\/div>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u7d30\u80de\u8272\u7d20c\u662f\u7dda\u7c92\u9ad4\u4e2d\u96fb\u5b50\u50b3\u905e\u93c8\u7684\u7d44\u5206\u3002 \u7d30\u80de\u8272\u7d20c\u7684\u8840\u7d05\u7d20\u7d44\u63a5\u53d7\u4f86\u81eabc1\u8907\u5408\u7269\u7684\u96fb\u5b50\u4e26\u5c07\u96fb\u5b50\u8f49\u79fb\u5230\u5fa9\u5408\u7269IV\u3002 \u7d30\u80de\u8272\u7d20c\u4e5f\u53c3\u8207\u7d30\u80de\u51cb\u4ea1\u7684\u8d77\u59cb\u3002 \u5728\u7d30\u80de\u8272\u7d20c\u91cb\u653e\u5230\u7d30\u80de\u8cea\u5f8c\uff0c\u86cb\u767d\u8cea\u7d50\u5408\u51cb\u4ea1\u86cb\u767d\u9176\u6fc0\u6d3b\u56e0\u5b50-1\uff08Apaf-1\uff09\u3002 \u7d30\u80de\u8272\u7d20c\u9084\u53ef\u4ee5\u50ac\u5316\u5e7e\u7a2e\u6c27\u5316\u9084\u539f\u53cd\u61c9\uff0c\u5982\u7fa5\u57fa\u5316\u548c\u82b3\u9999\u6c27\u5316\uff0c\u4e26\u901a\u904e\u6c27\u5316\u5404\u7a2e\u96fb\u5b50\u4f9b\u9ad4\u59822,2-\u9023\u6c2e\u57fa – \u96d9\uff083-\u4e59\u57fa\u82ef\u4e26\u567b\u5511\u5549-6-\u78fa\u9178\uff09\uff08ABTS\uff09\u986f\u793a\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\uff0c2- \u916e-4-\u786b\u4ee3\u7532\u57fa\u4e01\u9178\u548c4-\u6c28\u57fa\u5b89\u66ff\u6bd4\u6797\u3002 BioVision\u7684\u7d30\u80de\u8272\u7d20c ELISA\u8a66\u5291\u76d2\u662f\u4e00\u7a2esandwich ELISA\u6aa2\u6e2c\uff0c\u7528\u65bc\u5b9a\u91cf\u6e2c\u91cf\u8840\u6e05\u3001\u8840\u6f3f\u548c\u7d30\u80de\u57f9\u990a\u4e0a\u6e05\u6db2\u4e2d\u7684\u4eba\u7d30\u80de\u8272\u7d20c\u3002 \u984f\u8272\u5bc6\u5ea6\u8207\u5f9e\u6a23\u54c1\u4e2d\u6355\u7372\u7684\u4eba\u7d30\u80de\u8272\u7d20c\u7684\u91cf\u6210\u6bd4\u4f8b\u3002<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

\u4eba\u5fc3\u81df\u7d44\u7e54\u63d0\u53d6\u7269\u4e2d\u5929\u7136\u7d30\u80de\u8272\u7d20 C \u7684\u5167\u63d2\u6fc3\u5ea6<\/span><\/strong><\/span><\/div>\n
\n

\u57fa\u65bc 20 \u03bcg\/mL \u63d0\u53d6\u7269\u8ca0\u8377\u7684\u4eba\u5fc3\u81df\u7d44\u7e54\u63d0\u53d6\u7269\u3001\u57fa\u65bc 50 \u03bcg\/mL \u63d0\u53d6\u7269\u8ca0\u8377\u7684\u7d50\u8178\u7d44\u7e54\u63d0\u53d6\u7269\u548c\u57fa\u65bc 25 \u03bcg\/mL \u63d0\u53d6\u7269\u8ca0\u8377\u7684\u9aa8\u9abc\u808c\u7d44\u7e54\u63d0\u53d6\u7269\u7684\u5929\u7136\u7d30\u80de\u8272\u7d20 C \u7684\u5167\u63d2\u6fc3\u5ea6\u3002\u7d30\u80de\u8272\u7d20 C \u7684\u6fc3\u5ea6\u4e00\u5f0f\u5169\u4efd\u6e2c\u91cf\u4e26\u5f9e\u7d30\u80de\u8272\u7d20 C \u6a19\u6e96\u66f2\u7dda\u5167\u63d2\uff0c\u4e26\u91dd\u5c0d\u6a23\u54c1\u7a00\u91cb\u9032\u884c\u6821\u6b63\u3002\u7e6a\u88fd\u5167\u63d2\u7a00\u91cb\u56e0\u5b50\u6821\u6b63\u503c\uff08\u5e73\u5747\u503c +\/- SD\uff0cn=2\uff09\u3002\u5e73\u5747\u7d30\u80de\u8272\u7d20 C \u6fc3\u5ea6\u5728\u5fc3\u81df\u7d44\u7e54\u63d0\u53d6\u7269\u4e2d\u6e2c\u5b9a\u70ba 35.41 ng\/mL\uff0c\u5728\u7d50\u8178\u7d44\u7e54\u63d0\u53d6\u7269\u4e2d\u6e2c\u5b9a\u70ba 32.74 ng\/mL\uff0c\u5728\u9aa8\u9abc\u808c\u7d44\u7e54\u63d0\u53d6\u7269\u4e2d\u6e2c\u5b9a\u70ba 43.12 ng\/mL\u3002<\/p>\n

\"\"<\/p>\n

\u4eba\u548c\u5927\u9f20\u5fc3\u81df\u7d44\u7e54\u63d0\u53d6\u7269\u7684\u53cd\u61c9\u6027<\/strong><\/span><\/div>\n
\n

\u5176\u4ed6\u7269\u7a2e\u7684\u53cd\u61c9\u6027\u662f\u901a\u904e\u6e2c\u91cf\u4eba\u548c\u5927\u9f20\u5fc3\u81df\u7d44\u7e54\u63d0\u53d6\u7269\u6a23\u54c1\u7684 20 \u03bcg\/mL \u63d0\u53d6\u7269\u8ca0\u8377\uff0c\u5f9e\u4eba\u6a19\u6e96\u66f2\u7dda\u4e2d\u63d2\u5165\u86cb\u767d\u8cea\u6fc3\u5ea6\uff0c\u4e26\u5c07\u63d2\u5165\u6fc3\u5ea6\u8868\u793a\u70ba\u4eba\u5fc3\u81df\u4e2d\u86cb\u767d\u8cea\u6fc3\u5ea6\u7684\u767e\u5206\u6bd4\u4f86\u78ba\u5b9a\u7684\u7d44\u7e54\u63d0\u53d6\u7269\u3002\u5927\u9f20\u5fc3\u81df\u63d0\u53d6\u7269\u7684\u4ea4\u53c9\u53cd\u61c9\u88ab\u78ba\u5b9a\u70ba 100%\u3002\u7531\u65bc\u5927\u9f20\u548c\u5c0f\u9f20\u7d30\u80de\u8272\u7d20 C \u7684\u6c28\u57fa\u9178\u5e8f\u5217 100% \u76f8\u540c\uff0c\u56e0\u6b64\u53ef\u4ee5\u5047\u8a2d\u5c0f\u9f20\u7d30\u80de\u8272\u7d20 C \u5177\u6709\u76f8\u540c\u7684\u4ea4\u53c9\u53cd\u61c9\u6027\u3002<\/p>\n

\"\"<\/p>\n

\u4e9e\u7d30\u80de\u7d44\u5206\u4e2d\u7684\u7d30\u80de\u8272\u7d20 C \u5206\u4f48<\/strong><\/span><\/div>\n
\n

\u5728\u5b58\u5728\uff08\u8655\u7406\uff09\u6216\u4e0d\u5b58\u5728\uff08\u672a\u8655\u7406\uff091 \u00b5M \u661f\u5f62\u5b62\u83cc\u7d20\u7684\u60c5\u6cc1\u4e0b\u57f9\u990a 4 \u5c0f\u6642\u7684 3.7×103 HeLa \u7d30\u80de\u548c\u5b8c\u6574\u7d30\u80de\u7684\u4e9e\u7d30\u80de\u7d44\u5206\u4e2d\u7d30\u80de\u8272\u7d20 C \u7684\u5206\u4f48\u6bd4\u8f03\u3002\u8655\u7406\u5f8c\u76f4\u63a5\u6536\u96c6\u7d30\u80de\uff0c\u4e26\u4f7f\u7528\u7d30\u80de\u5206\u7d1a\u5206\u96e2\u8a66\u5291\u76d2 ( ab109719)<\/a>\u88fd\u5099\u4e9e\u7d30\u80de\u7d44\u5206).\u00a0\u5982\u7b2c 11.10 \u7bc0\u6240\u8ff0\u8655\u7406\u5206\u6578\u3002\u4e26\u5316\u9a57\u3002\u7d30\u80de\u8272\u7d20 C \u7684\u6fc3\u5ea6\u5728\u4e09\u500b\u4e0d\u540c\u7a00\u91cb\u5ea6\u7684\u7d1a\u5206\u6a23\u54c1\u4e2d\u4e00\u5f0f\u5169\u4efd\u6e2c\u91cf\uff0c\u4e26\u5f9e\u7d30\u80de\u8272\u7d20 C \u6a19\u6e96\u66f2\u7dda\u5167\u63d2\u3002\u7e6a\u88fd\u5167\u63d2\u503c\uff08\u5e73\u5747\u503c +\/- SD\uff0cn=3\uff09\u3002\u5e73\u5747\u7d30\u80de\u8272\u7d20 C \u6fc3\u5ea6\u5728\u8655\u7406\u904e\u7684\u80de\u8cea\u6eb6\u81a0\u90e8\u5206\u4e2d\u78ba\u5b9a\u70ba 171.4 ng\/mL\uff0c\u5728\u8655\u7406\u904e\u7684\u7dda\u7c92\u9ad4\u90e8\u5206\u4e2d\u70ba 242.8\uff0c\u5728\u8655\u7406\u904e\u7684\u5168\u7d30\u80de\u6a23\u54c1\u4e2d\u70ba 562.0\uff0c\u5728\u672a\u8655\u7406\u7684\u7dda\u7c92\u9ad4\u90e8\u5206\u4e2d\u70ba 407.2 ng\/mL\uff0c\u5728\u8655\u7406\u904e\u7684\u7dda\u7c92\u9ad4\u90e8\u5206\u4e2d\u70ba 558.9 ng\/mL\u672a\u7d93\u8655\u7406\u7684\u5168\u7d30\u80de\u6a23\u672c\u3002\u5728\u672a\u8655\u7406\u7684\u7d30\u80de\u8cea\u90e8\u5206\u548c\u5169\u500b\u6838\u90e8\u5206\u4e2d\u90fd\u6aa2\u6e2c\u4e0d\u5230\u7d30\u80de\u8272\u7d20 C\u3002<\/p>\n<\/div>\n<\/div>\n<\/div>\n

\"\"<\/p>\n

\u4eba\u63d0\u53d6\u7269\u6a23\u54c1\u4e2d\u5929\u7136\u7d30\u80de\u8272\u7d20 C \u7684\u5167\u63d2\u6fc3\u5ea6<\/strong><\/span><\/div>\n
\n

\u4eba\u9ad4\u63d0\u53d6\u7269\u6a23\u54c1\u4e2d\u5929\u7136\u7d30\u80de\u8272\u7d20 C \u7684\u5167\u63d2\u6fc3\u5ea6\u3002\u7d30\u80de\u8272\u7d20 C \u7684\u6fc3\u5ea6\u5728\u4e09\u7a2e\u4e0d\u540c\u7684\u7a00\u91cb\u5ea6\u4e2d\u4e00\u5f0f\u5169\u4efd\u6e2c\u91cf\uff0c\u4e26\u5f9e\u7d30\u80de\u8272\u7d20 C \u6a19\u6e96\u66f2\u7dda\u5167\u63d2\u4e26\u91dd\u5c0d\u6a23\u54c1\u7a00\u91cb\u9032\u884c\u6821\u6b63\u3002\u5167\u63d2\u7a00\u91cb\u56e0\u5b50\u6821\u6b63\u503c\u4ee5\u6bcf\u6beb\u514b\u63d0\u53d6\u7269\u7684\u7d30\u80de\u8272\u7d20 C ng \u70ba\u55ae\u4f4d\u7e6a\u88fd\uff08\u5e73\u5747\u503c +\/- SD\uff0cn=3\uff09\u3002\u7d30\u80de\u8272\u7d20 C \u6fc3\u5ea6\u6e2c\u5b9a\u70ba\u5fc3\u81df\u7d44\u7e54\u63d0\u53d6\u7269 1716 ng\/mg\uff0c\u7d50\u8178\u7d44\u7e54\u63d0\u53d6\u7269 670.1 ng\/mg\uff0c\u9aa8\u9abc\u808c\u7d44\u7e54\u63d0\u53d6\u7269 1689 ng\/mg\uff0cHepG2 \u7d30\u80de\u63d0\u53d6\u7269 924.2 ng\/mg\uff0cPC-1658 ng\/mg 3 \u7d30\u80de\u63d0\u53d6\u7269\uff0c\u4ee5\u53ca THP-1 \u7d30\u80de\u63d0\u53d6\u7269\u6a23\u54c1\u4e2d\u7684 386.2 ng\/mg\u3002<\/p>\n<\/div>\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\n\u00a0ABCAM\u8ca8\u865f ab221832 (E4286-100)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\n\u2022 Detection method- Absorbance (450 nm)
\n\u2022 Species reactivity- Human
\n\u2022 Application- quantitative measurement of Cytochrome c in human serum, plasma, tissue homogenates and other biological fluids.<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (450 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nHuman<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThis ELISA kit is used for quantitative measurement of Cytochrome c in human serum, plasma, tissue homogenates and other biological fluids.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Easy, convenient and time-saving method to measure the level of Cytochrome c in human serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
\n\u2022 Detection Range: 78.12 – 5000 pg\/ml
\n\u2022 Sensitivity: < 46.87 pg\/ml
\n\u2022 Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
\n\u2022 Cross Reactivity: No significant cross-reactivity or interference between this analyte and its analogues was observed.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Micro ELISA Plate
\n\u2022 Lyophilized Standard
\n\u2022 Sample \/ Standard dilution buffer
\n\u2022 Biotin- detection antibody (Concentrated)
\n\u2022 Antibody dilution buffer
\n\u2022 HRP-Streptavidin Conjugate (SABC)
\n\u2022 SABC dilution buffer
\n\u2022 TMB substrate
\n\u2022 Stop Solution
\n\u2022 Wash buffer (25X)
\n\u2022 Plate sealers<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u7d30\u80de\u8272\u7d20c\uff08\u4eba\uff09ELISA\u8a66\u5291\u76d2\u00a0\u00a0|\u00a0\u8ca8\u865f\u00a0ABCAM\u8ca8\u865f ab221832 (E4286-100) Cyt […]<\/p>\n","protected":false},"author":3,"featured_media":37625,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261],"tags":[5506],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40614"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=40614"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40614\/revisions"}],"predecessor-version":[{"id":40618,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40614\/revisions\/40618"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/37625"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=40614"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=40614"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=40614"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}