{"id":40469,"date":"2023-03-24T15:18:37","date_gmt":"2023-03-24T07:18:37","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=40469"},"modified":"2024-04-17T11:07:38","modified_gmt":"2024-04-17T03:07:38","slug":"%e9%85%aa%e6%b0%a8%e9%85%b8%e9%85%b6%e6%b4%bb%e6%80%a7%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-tyrosinase-activity-assay-kit-attogene%e8%b2%a8%e8%99%9fez2017-01-30-%c2%b5u","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=40469","title":{"rendered":"\u916a\u6c28\u9178\u9176(Tyrosinase)\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | Tyrosinase Activity Assay Kit ATTOGENE\u8ca8\u865fEZ2017-01 (30 \u00b5U)"},"content":{"rendered":"

\u916a\u6c28\u9178\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | Tyrosinase Activity Assay Kit ATTOGENE\u8ca8\u865fEZ2017-01 (30 \u00b5U)<\/a><\/span><\/strong><\/p>\n

For rapid, sensitive and accurate screening of potential Tyrosinase inhibitors<\/span><\/p>\n

\u916a\u6c28\u9178\u9176 (Tyrosinase, EC 1.14.18.1) \u662f\u4e00\u7a2e\u9285\u7d50\u5408\u9176(copper-binding enzyme )\uff0c\u5728\u5f9e\u7d30\u83cc(bacteria )\u548c\u771f\u83cc(fungi )\u5230\u54fa\u4e73\u52d5\u7269(\u00a0mammals)\u7684\u5ee3\u6cdb\u7269\u7a2e\u4e2d\u90fd\u6709\u8868\u9054\u3002\u5b83\u53c3\u8207\u9ed1\u8272\u7d20\u5408\u6210\u9014\u5f91(melanin synthesis pathway)\u7684\u5169\u500b\u9023\u7e8c\u53cd\u61c9\uff1b\u9996\u5148\u662f\u55ae\u915a(monophenol )\u7684\u7fa5\u57fa\u5316(hydroxylation)\uff0c\u5176\u6b21\u662f\u9130\u4e8c\u915a\u5411\u918c(ortho-diphenol )\u7684\u8f49\u5316\u3002\u918c\u7136\u5f8c\u7d93\u6b77\u4e00\u7cfb\u5217\u53cd\u61c9\uff0c\u5305\u62ec\u805a\u5408(polymerization)\u5f62\u6210\u9ed1\u8272\u7d20(melanin)\u3002\u9ed1\u8272\u7d20\uff08melanin\uff09\u5728\u9ed1\u8272\u7d20\u7d30\u80de\uff08melanocytes\uff09\u7684\u751f\u7269\u5408\u6210\u7684\u9014\u5f91\u662f\u7531\uf919\u80fa\u9178 \uff08tyrosine\uff09\u8207\uf919\u80fa\u9178\u9176\uff08tyrosinase<\/span>\uff09\u7684\u4f5c\u7528\u958b\u59cb\u3002\u73fe\u4eca\u5df2\u77e5\u7684\u7f8e\u767d\u6210\u5206\u4e4b\u4e2d\uff0c\u5c0d\u65bc\uf919\u80fa\u9178\u9176\u6d3b\u6027\u6291\u5236\u7684\u5c08\u4e00\u6291\u5236\u5291\uff0c\u901a\u5e38\u5177\u6709\u8207\uf919\u80fa\u9178\u76f8\u4f3c\u7684\u7d50\u69cb\uff0c\u5728\u9ed1\u8272\u7d20\u7d30\u80de\u5167\u9032\ufa08 \u7af6\u722d\u6027\u6291\u5236\uf919\u80fa\u9178\u9176\u4ee5\u9054\u6e1b\u5c11\u9ed1\u8272\u7d20\u751f\u6210\u3002<\/p>\n

\u916a\u6c28\u9178\u9176(Tyrosinase )\u5f15\u8d77\u8fb2\u696d\u5de5\u696d\u7684\u6975\u5927\u8208\u8da3\uff0c\u56e0\u70ba\u5b83\u6703\u5f15\u8d77\u6c34\u679c(fruits)\u3001\u852c\u83dc(vegetable )\u548c\u8611\u83c7(mushrooms)\u7684\u8910\u8b8a\uff0c\u4ee5\u53ca\u5f15\u8d77\u76ae\u819a\u8b8a\u9ed1(skin darkening)\u7684\u5316\u599d\u54c1\u5de5\u696d\u3002\u56e0\u6b64\uff0c\u916a\u6c28\u9178\u9176\u6291\u88fd\u5291(\u00a0tyrosinase inhibitors)\u7684\u958b\u767c\u548c\u7be9\u9078(screening)\u5c0d\u65bc\u8272\u7d20\u6c89\u8457\u904e\u5ea6(hyperpigmentation)\u548c\u9ec3\u8910\u6591(melasma)\u7b49\u75c5\u75c7\u975e\u5e38\u6709\u7528\u3002\u9ed1\u8272\u7d20\u7624(melanoma)\u4e2d\u7684\u916a\u6c28\u9178\u9176\u6d3b\u6027(Tyrosinase activity )\u986f\u8457\u589e\u52a0\u3002\u56e0\u6b64\uff0c\u6aa2\u6e2c\u916a\u6c28\u9178\u9176\u6d3b\u6027(tyrosinase activity )\u6709\u671b\u6210\u70ba\u9ed1\u8272\u7d20\u7624(melanoma)\u7684\u7279\u7570\u6027\u8a3a\u65b7\u6e2c\u8a66(specific diagnostic test )\uff0c\u4e26\u53ef\u7528\u65bc\u76e3\u6e2c\u60a3\u8005\u5c0d\u9ed1\u8272\u7d20\u7624\u6cbb\u7642(melanoma treatments)\u7684\u53cd\u61c9\u3002\u00a0environmental<\/a>,\u00a0Food Safety<\/a>,\u00a0life science<\/a>,\u00a0pesticide<\/a>,\u00a0Tyrosinase Activity<\/a><\/p>\n

\u916a\u6c28\u9178\u9176\u6d3b\u6027\u6e2c\u5b9a\u539f\u7406\u4ecb\u7d39<\/span><\/strong><\/span><\/p>\n

\u916a\u6c28\u9178\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2(Tyrosinase Activity Assay)\u662f\u4e00\u7a2e\u7c21\u55ae\u7684\u57fa\u65bc\u677f\u7684\u4e00\u6b65\u5f0f\u6e2c\u5b9a\u6cd5\uff0c\u7528\u65bc\u6e2c\u91cf\u751f\u7269\u6a23\u54c1\u4e2d\u7684\u916a\u6c28\u9178\u9176\u6d3b\u6027(\u00a0tyrosinase activity)\u3002\u5728\u6b64\u6e2c\u5b9a\u4e2d\uff0c\u916a\u6c28\u9178\u9176(tyrosinase)\u50ac\u5316\u915a\u985e\u5e95\u7269\u8f49\u5316\u70ba\u918c\u4e2d\u9593\u9ad4(Quinone intermediate)\uff0c\u5f8c\u8005\u8207\u916a\u6c28\u9178\u589e\u5f37\u5291(tyrosine enhancer)\u53cd\u61c9\uff0c\u5f62\u6210\u9ad8\u5ea6\u7a69\u5b9a\u7684\u9aee\u8272\u5718(chromophore)\uff0c\u5728 520 nm \u8655\u6709\u5438\u5149\u5ea6\u3002\u8a72\u6e2c\u5b9a\u6cd5\u53ef\u6aa2\u6e2c\u751f\u7269\u6a23\u54c1\u4e2d\u4f4e\u81f3 30 \u00b5U \u7684\u916a\u6c28\u9178\u9176(\u00a0Tyrosinase )\u3002<\/p>\n

Tyrosinase (EC 1.14.18.1) is a copper-binding enzyme that is expressed across a vast range of species ranging from bacteria and fungi to mammals. It is involved in two sequential reactions of the melanin synthesis pathway: first being the hydroxylation of a monophenol and second the conversion of an ortho-diphenol to a quinone. Quinone then undergoes a series of reactions including polymerization to form melanin. Tyrosinase is of great interest to the agriculture industry since it causes browning of fruits, vegetable and mushrooms, as well as to the cosmetic industry as it causes skin darkening. Development and screening of tyrosinase inhibitors, therefore, is very useful for conditions such as hyperpigmentation and melasma. Tyrosinase activity is significantly increased in melanoma. Therefore, the detection of tyrosinase activity could be promising as a specific diagnostic test for melanoma and may be useful in monitoring patient response to melanoma treatments. This Tyrosinase Activity Assay Kit is a simple one-step, plate-based assay for the measurement of tyrosinase activity in biological samples. In this assay, tyrosinase catalyzes the conversion of a phenolic substrate to a Quinone inter-mediate, which reacts with the tyrosine enhancer forming a highly stable chromophore with absorbance at 520 nm. The assay can detect as low as 30 \u03bcU Tyrosinase in biological samples.<\/p>\n

\"\"<\/p>\n

The kit provides a rapid, simple, sensitive, and reliable test suitable for screening of tyrosinase concen-tration in samples.<\/span><\/strong><\/p>\n

\u8a72\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u5feb\u901f(rapid)\u3001\u7c21\u55ae(simple)\u3001\u9748\u654f(sensitive)\u4e14\u53ef\u9760\u7684\u6aa2\u6e2c\u65b9\u6cd5\uff0c\u9069\u7528\u65bc\u6a23\u54c1\u4e2d\u916a\u6c28\u9178\u9176\u6fc3\u5ea6(tyrosinase concen-tration)\u7684\u00a0screening\u3002\u916a\u6c28\u9178\u9176\u6fc3\u5ea6\u53ef\u4ee5\u901a\u904e\u8b80\u53d6\u53cd\u61c9\u5728 520nm \u8655\u7684\u5438\u5149\u5ea6\u4f86\u6e2c\u91cf\uff0c\u4f7f\u7528\u8a66\u5291\u76d2\u4e2d\u63d0\u4f9b\u7684\u6a19\u6e96(\u00a0standards)\u751f\u6210\u6a19\u6e96\u66f2\u7dda(standard curve)\u4e26\u4f7f\u7528\u7dda\u6027\u56de\u6b78\u5206\u6790\u672a\u77e5\u6a23\u54c1\u7684\u6fc3\u5ea6\u3002<\/p>\n

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Set up reaction in 96 well plate from the master mix by:<\/span><\/strong><\/p>\n

\"\"
\n\u2022 Step 1. Aliquoting 223\u03bcL of the master mix into each well of the 96 well plate.
\n\u2022 Step 2. Add 40\u03bcL of Sample, Tyrosinase Lysis Buffer or Tyrosinase Positive Control
\n\u2022 Step 3. Add 7\u03bcL of Substrate [S]
\n\u2022 Step 4. Mix the components in the tube by pipetting up and down 3-4 times
\n\u2022 Step 5. Incubate at room temperature for 25 minutes.
\n\u2022 Step 6. Read the plate by measuring absorbance at 520nm.<\/p>\n

Concentration Calculation:<\/span><\/strong><\/span>
\nStandard curve can be constructed by plotting the mean relative absorbance (%) obtained from each reference standard against its concentration in units on a logarithmic curve.
\nRelative absorbance (%) = absorbance standard (or sample) x 100 absorbance zero standard
\nUse the mean relative absorbance values for each sample to determine the corresponding concen-tration of the sample from the standard curve.<\/p>\n

<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nEZ2017-01<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\n\u2022 Detection method- Absorbance (520 nm)
\n\u2022 Applications- Measurement of Tyrosinase Activity in various tissue\/cell lysates
\n– Analysis of Tyrosinase activity in food chemistry\/skin-related research<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (520 nm)<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\u2022 Measurement of Tyrosinase Activity in various tissue\/cell lysates
\n\u2022 Analysis of Tyrosinase activity in food chemistry\/skin-related research<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 First non-radioactive assay in the market
\n\u2022 Simple, rapid & convenient
\n\u2022 The assay can detect Tyrosinase Activity less than 30 \u00b5U in biological samples.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u54c1\u724c\u4ecb\u7d39_\u5de5\u4f5c\u5340\u57df<\/p>\n

Attogene \u53f0\u7063\u4ee3\u7406\/Attogene \u53f0\u7063\u7d93\u92b7<\/strong><\/span><\/p>\n

10nm Colloidal Gold<\/a>\u300130nm Colloidal Gold<\/a>\u3001\u00a0<\/a>40nm Colloidal Gold\u00a0<\/a>\u3001<\/span><\/p>\n

Attogene\u00a0\u63d0\u4f9b\u5074\u6d41\u5206\u6790\u958b\u767c(Lateral Flow Assay)\u5948\u7c73\u91d1\u7c92\u5b50(\u00a0Colloidal Gold)<\/span><\/strong><\/span><\/p>\n

\"ATTOGENE<\/p>\n

\u7528\u65bc\u6a6b\u5411\u6d41\u52d5(Lateral Flow Assay)\u7684\u81a0\u9ad4\u91d1(\u00a0Colloidal Gold)\u662f\u4e00\u7a2e\u9ad8\u5ea6\u7a69\u5b9a\u4e14\u5747\u52fb\u7684\u91d1\u7d0d\u7c73\u7c92\u5b50(gold nanoparticles)\uff0c\u81a0\u9ad4\u91d1(\u00a0Colloidal Gold)\u7528\u65bc\u6297\u9ad4\u7684\u50b3\u7d71\u88ab\u52d5\u7d50\u5408\uff0c\u00a0\u7121\u5857\u5c64\u91d1\u7d0d\u7c73\u7c92\u5b50\u65bcPassive conjugation\u88ab\u52d5\u5171\u8edb\u9032\u884c\u4e86\u512a\u5316\uff0c\u53ef\u61c9\u7528\u65bc lateral flow tests\u5074\u5411\u6d41\u52d5\u6e2c\u8a66\u3002\u63d0\u4f9b\u5f62\u72c0\u5c3a\u5bf8\u5206\u4f48\u6700\u5e73\u5747\u4e4b\u5948\u7c73\u91d1\u7c92\u5b50\u3002\u9069\u7528\u65bc\uff1a\u86cb\u767d\u8cea\u5438\u9644\u3001\u5be1\u6838\u82f7\u9178\u5438\u9644\u3001\u8868\u9762\u4fee\u98fe\u3002Attogene\u5948\u7c73\u91d1\u6eb6\u6db2\u7279\u9ede:\u00a0\u5f62\u72c0\u6700\u4e00\u81f4\u3001\u5c3a\u5bf8\u5206\u4f48\u6700\u5e73\u574710nm \u00b1 1.5nm\uff0c\u5747\u52fb\u7684\u5f62\u72c0\u78ba\u4fdd\u6297\u9ad4\u7684\u4e00\u81f4\u7d50\u5408\uff0c\u78ba\u4fdd\u53ef\u9760\u7684\u5206\u6790\u7d50\u679c\u3002\u9ad8\u6297\u9ad4\u7d50\u5408\u7387\u4ee5\u9054\u6700\u4f73\u9748\u654f\u5ea6\u3002\u6bcf\u627910nm\u81a0\u9ad4\u91d1: \u5e73\u5747\u76f4\u5f91\uff1a8.5-11.5nm<\/p>\n

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\u53ef\u63d0\u4f9b 1 OD \u81f3 100 OD \u7684\u7bc4\u570d(\u7c92\u5b50\u6fc3\u5ea62.5- 8.6 x\u00a01012<\/sup>\/mL)\u3002\u5076\u806f\u7269(\u00a0conjugate )\u7684\u8cea\u91cf\u548c\u6027\u80fd\u5c0d\u65bc\u6210\u529f\u7684\u6a6b\u5411\u6d41\u52d5\u6e2c\u8a66\u751f\u7522(lateral flow test manufacturing)\u81f3\u95dc\u91cd\u8981\u3002ATTOGENE\u7522\u54c1\u5728\u7f8e\u570b\u88fd\u9020\uff0c\u4e26\u5728\u6700\u5148\u9032\u7684\u88fd\u9020\u8a2d\u65bd\u4e2d\u751f\u7522\uff0c\u53ef\u5be6\u73fe\u5feb\u901f\u5468\u8f49\u6642\u9593\uff0c\u540c\u6642\u78ba\u4fdd\u6279\u6b21\u9593\u7684\u4e00\u81f4\u6027\u548c\u53ef\u9760\u6027\u3002\u5efa\u8b70\u7684\u5132\u5b58\u548c\u8655\u7406\u7a0b\u5e8f\uff1a\u5132\u5b58\u5728 2 \u2013 25o<\/sup>C. \u4e0d\u8981\u51cd\u7d50\u3002\u51b7\u51cd\u6703\u5c0e\u81f4\u4e0d\u53ef\u9006\u7684\u805a\u96c6\u9846\u7c92\u4e26\u7834\u58de\u7522\u54c1\u3002\u7f6e\u65bc\u5ba4\u6eab\u4e26\u5287\u70c8\u6416\u6643 30 \u79d2\u4ee5\u78ba\u4fdd\u9846\u7c92\u5145\u5206\u6bcf\u6b21\u4f7f\u7528\u524d\u5206\u6563\u4f7f\u7528\u3002\u6bcfml\u7684\u5948\u7c73\u91d1\u7c92\u5b502.5- 8.6 x\u00a01012<\/sup>\/mL\u3002\u81a0\u9ad4\u91d1\u6fc3\u5ea6\u00a0(mg\/mL)\u70ba5.7- 6.0 x\u00a010-2<\/sup>\u3002Molar Concentration (moles\/liter)0.4-1.4 x\u00a010-8<\/sup><\/p>\n

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\n

Specifications 1OD 40nm Gold (1L)<\/span><\/strong><\/span><\/p>\n<\/div>\n\n\n\n\n\n\n
Number of particles\/mL<\/td>\n5.2-7.2\u00a0x 1010<\/sup><\/em><\/td>\n<\/tr>\n
Gold Concentration (mg\/mL)<\/td>\n3.7-4.2 x\u00a010-2<\/sup><\/em><\/td>\n<\/tr>\n
Molar Concentration (moles\/liter)<\/td>\n8-12 x\u00a010-11<\/sup><\/em><\/td>\n<\/tr>\n
Particle Diameter<\/td>\n40 nm \/- 1.5<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Related products:<\/span><\/strong><\/span><\/p>\n

AU2012-01: 10nm Colloidal Gold (1O.D, 1L) >>\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/p>\n

AU2013-01: 30nm Colloidal Gold\u00a0(1O.D, 1L)\u00a0>>\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/p>\n

AU2014-01: 40nm Colloidal Gold\u00a0(1O.D, 1L)\u00a0>>\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/p>\n

AU2015-01: 60nm Colloidal Gold\u00a0(1O.D, 1L)\u00a0>>\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/p>\n

\"\"<\/p>\n

40nm\u5948\u7c73\u91d1\u6eb6\u6db2\u7279\u9ede: (1O.D\/1\u516c\u5347, \u8ca8\u865fAU2014-01)<\/span><\/strong><\/span><\/p>\n

\u5f62\u72c0\u6700\u4e00\u81f4\u3001\u5c3a\u5bf8\u5206\u4f48\u6700\u5e73\u574740nm \u00b1 2.5nm\uff0c\u5747\u52fb\u7684\u5f62\u72c0\u78ba\u4fdd\u6297\u9ad4\u7684\u4e00\u81f4\u7d50\u5408\uff0c\u78ba\u4fdd\u53ef\u9760\u7684\u5206\u6790\u7d50\u679c\u3002\u9ad8\u6297\u9ad4\u7d50\u5408\u7387\u4ee5\u9054\u6700\u4f73\u9748\u654f\u5ea6\u3002\u6bcf\u627940nm\u81a0\u9ad4\u91d1(1 O.D): \u5e73\u5747\u76f4\u5f91\uff1a37.5-42.5nm<\/p>\n

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40 nm\u5948\u7c73\u91d1<\/span>\u9846\u7c92(1O.D\/1\u516c\u5347, \u8ca8\u865fAU2014-01)<\/span>\u7684\u7d2b\u5916-\u53ef\u898b\u5149\u8b5c<\/strong><\/span><\/p>\n

\n

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10nm\u5948\u7c73\u91d1\u6eb6\u6db2\u7279\u9ede: (1O.D\/1\u516c\u5347, AU2012-01)<\/strong><\/span><\/p>\n

\u5f62\u72c0\u6700\u4e00\u81f4\u3001\u5c3a\u5bf8\u5206\u4f48\u6700\u5e73\u574710nm \u00b1 1.5nm\uff0c\u5747\u52fb\u7684\u5f62\u72c0\u78ba\u4fdd\u6297\u9ad4\u7684\u4e00\u81f4\u7d50\u5408\uff0c\u78ba\u4fdd\u53ef\u9760\u7684\u5206\u6790\u7d50\u679c\u3002\u9ad8\u6297\u9ad4\u7d50\u5408\u7387\u4ee5\u9054\u6700\u4f73\u9748\u654f\u5ea6\u3002\u6bcf\u627910nm\u81a0\u9ad4\u91d1(1 O.D): \u5e73\u5747\u76f4\u5f91\uff1a8.5-11.5nm\u3001<\/p>\n

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10 nm\u5948\u7c73\u91d1<\/span>\u9846\u7c92 \u00a0(1O.D\/1\u516c\u5347,\u00a0AU2012-01)\u7684\u7d2b\u5916-\u53ef\u898b\u5149\u8b5c<\/strong><\/span><\/p>\n

\n

\"\"<\/p>\n

30nm\u5948\u7c73\u91d1\u6eb6\u6db2\u7279\u9ede: (1O.D\/1\u516c\u5347, \u8ca8\u865fAU2013-01)<\/span><\/strong><\/span><\/p>\n

\u5f62\u72c0\u6700\u4e00\u81f4\u3001\u5c3a\u5bf8\u5206\u4f48\u6700\u5e73\u574730nm \u00b1 1.5nm\uff0c\u5747\u52fb\u7684\u5f62\u72c0\u78ba\u4fdd\u6297\u9ad4\u7684\u4e00\u81f4\u7d50\u5408\uff0c\u78ba\u4fdd\u53ef\u9760\u7684\u5206\u6790\u7d50\u679c\u3002\u9ad8\u6297\u9ad4\u7d50\u5408\u7387\u4ee5\u9054\u6700\u4f73\u9748\u654f\u5ea6\u3002\u6bcf\u627930nm\u81a0\u9ad4\u91d1(1 O.D): \u5e73\u5747\u76f4\u5f91\uff1a28.5-31.5nm<\/p>\n

Specifications 1OD 30nm Gold (1L)<\/span><\/strong><\/p>\n\n\n\n\n\n\n
\u2022 Number of particles\/mL<\/td>\n1.3-2 x\u00a010<\/em>11<\/sup><\/em><\/td>\n<\/tr>\n
\u2022 Gold Concentration (mg\/mL)<\/td>\n4.2-4.7 x\u00a010<\/em>-2<\/sup><\/em><\/td>\n<\/tr>\n
\u2022 Molar Concentration (moles\/liter)<\/td>\n2.2-3.3 x\u00a010<\/em>-10<\/sup><\/em><\/td>\n<\/tr>\n
\u2022 Particle Diameter<\/td>\n30 nm \/-1.5<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\"<\/p>\n

30 nm\u5948\u7c73\u91d1\u9846\u7c92 \u00a0(1O.D\/1\u516c\u5347,\u00a0AU2013-01)\u7684\u7d2b\u5916-\u53ef\u898b\u5149\u8b5c<\/strong><\/span><\/p>\n

\n

\"\"<\/p>\n

60nm\u5948\u7c73\u91d1\u6eb6\u6db2\u7279\u9ede: (1O.D\/1\u516c\u5347, \u8ca8\u865fAU2015-01)<\/span><\/strong><\/span><\/p>\n

\u5f62\u72c0\u6700\u4e00\u81f4\u3001\u5c3a\u5bf8\u5206\u4f48\u6700\u5e73\u574760nm \u00b1 4.5nm\uff0c\u5747\u52fb\u7684\u5f62\u72c0\u78ba\u4fdd\u6297\u9ad4\u7684\u4e00\u81f4\u7d50\u5408\uff0c\u78ba\u4fdd\u53ef\u9760\u7684\u5206\u6790\u7d50\u679c\u3002\u9ad8\u6297\u9ad4\u7d50\u5408\u7387\u4ee5\u9054\u6700\u4f73\u9748\u654f\u5ea6\u3002\u6bcf\u627960nm\u81a0\u9ad4\u91d1(1 O.D): \u5e73\u5747\u76f4\u5f91\uff1a65.5-64.5nm<\/p>\n

Specifications 1OD 60nm Gold (1L)<\/h4>\n\n\n\n\n\n\n
Number of particles\/mL<\/td>\n1.5-2 x 1010<\/sup><\/em><\/td>\n<\/tr>\n
Gold Concentration (mg\/mL)<\/td>\n3.6-4.1 x\u00a010-2<\/sup><\/em><\/td>\n<\/tr>\n
Molar Concentration (moles\/liter)<\/td>\n2.5-3.4 x\u00a010-11<\/sup><\/em><\/td>\n<\/tr>\n
Particle Diameter<\/td>\n40 nm \/- 1.5<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\"<\/p>\n

60 nm\u5948\u7c73\u91d1\u9846\u7c92 \u00a0(1O.D\/1\u516c\u5347,\u00a0AU2015-01)\u7684\u7d2b\u5916-\u53ef\u898b\u5149\u8b5c<\/strong><\/span><\/p>\n

\n

\"gold_nanoparticles_new\"<\/p>\n

ATTOGENE\u63d0\u4f9b 1 OD \u81f3 100 OD \u7684\u7bc4\u570d(\u4ee540nm\u5948\u7c73\u91d1\u7c92\u5b50\u70ba\u4f8b\uff0c\u7c92\u5b50\u6fc3\u5ea62.5- 8.6 x\u00a01012<\/sup>\/mL)\u3002\u5076\u806f\u7269(\u00a0conjugate )\u7684\u8cea\u91cf\u548c\u6027\u80fd\u5c0d\u65bc\u6210\u529f\u7684\u6a6b\u5411\u6d41\u52d5\u6e2c\u8a66\u751f\u7522(lateral flow test manufacturing)\u81f3\u95dc\u91cd\u8981\u3002<\/p>\n

\u5efa\u8b70\u7684\u5132\u5b58\u548c\u8655\u7406\u7a0b\u5e8f\uff1a<\/span><\/strong><\/span><\/p>\n

\u5132\u5b58\u5728 2 \u2013 25o<\/sup>C. \u4e0d\u8981\u51cd\u7d50\u3002\u51b7\u51cd\u6703\u5c0e\u81f4\u4e0d\u53ef\u9006\u7684\u805a\u96c6\u9846\u7c92\u4e26\u7834\u58de\u7522\u54c1\u3002\u7f6e\u65bc\u5ba4\u6eab\u4e26\u5287\u70c8\u6416\u6643 30 \u79d2\u4ee5\u78ba\u4fdd\u9846\u7c92\u5145\u5206\u6bcf\u6b21\u4f7f\u7528\u524d\u5206\u6563\u4f7f\u7528\u3002\u4ee540nm\u5948\u7c73\u91d1\u7c92\u5b50\u70ba\u4f8b\uff0c\u6bcfml\u7684\u5948\u7c73\u91d1\u7c92\u5b502.5- 8.6 x\u00a01012<\/sup>\/mL\u3002\u81a0\u9ad4\u91d1\u6fc3\u5ea6\u00a0(mg\/mL)\u70ba5.7- 6.0 x\u00a010-2<\/sup>\u3002Molar Concentration (moles\/liter)0.4-1.4 x\u00a010-8<\/sup><\/p>\n

\"\"<\/p>\n

\u5948\u7c73\u91d1\uff08\u53c8\u7a31\u70ba\u81a0\u9ad4\u91d1\uff09\uff0c\u5e38\u4ee5\u6eb6\u6db2\u578b\u614b\u5448\u73fe\u3002\u7576\u5948\u7c73\u91d1\u7c92\u5b50\u8d8a\u5c0f\uff08<100nm\uff09\u5247\u5448\u73fe\u7d05\u8272\u6eb6\u6db2\u3002\u53cd\u4e4b\uff08>100nm\uff09\u5247\u8d8a\u504f\u5411\u85cd\u7d2b\u8272\u3002\u5948\u7c73\u91d1\u7c92\u5b50\u7528\u9014\u5ee3\u6cdb\uff0c\u53ef\u61c9\u7528\u65bc\u6a6b\u5411\u6d41\u5206\u6790\u3001\u986f\u5fae\u5f71\u50cf\u3001\u6d41\u5f0f\u7d30\u80de\u3001\u840a\u66fc\u5149\u8b5c\u3001\u4ee5\u53ca\u52d5\u614b\u5149\u6563\u5c04\uff08DLS\uff09\u3002\u5728\u9019\u4e9b\u61c9\u7528\u4e2d\uff0c\u5948\u7c73\u91d1\u7c92\u5b50\u7684\u5927\u5c0f\u662f\u975e\u5e38\u91cd\u8981\u7684\u5f71\u97ff\u95dc\u9375\u3002\u96d6\u7136\u5948\u7c73\u91d1\u7684\u4f86\u6e90\u591a\u7a2e\uff08\u81ea\u88fdor\u5546\u54c1\u5316\u7522\u54c1\uff09\uff0cATTOGENE\u7522\u54c1\u5728\u7f8e\u570b\u88fd\u9020\uff0c\u4e26\u5728\u6700\u5148\u9032\u7684\u88fd\u9020\u8a2d\u65bd\u4e2d\u751f\u7522\uff0c\u53ef\u5be6\u73fe\u5feb\u901f\u5468\u8f49\u6642\u9593\uff0c\u540c\u6642\u78ba\u4fdd\u6279\u6b21\u9593\u7684\u4e00\u81f4\u6027\u548c\u53ef\u9760\u6027\u3002<\/p>\n

Colloidal gold applications<\/span><\/strong><\/span><\/h3>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Application<\/strong>
\n<\/strong><\/td>\n
\nSize<\/strong><\/td>\n		\u00a0 \u00a0Shape<\/strong><\/td>\n<\/tr>\n
10<\/strong><\/td>\n20<\/strong><\/td>\n40<\/strong><\/td>\n80<\/strong><\/td>\n<\/tr>\n
Lateral Flow<\/strong><\/a><\/td>\n<\/td>\n\u00a0\u25cf<\/td>\n\u00a0 \u25cf<\/td>\n\u00a0\u25cf<\/td>\nSphere<\/td>\n<\/tr>\n
Western Blots<\/strong><\/a><\/td>\n<\/td>\n\u00a0\u25cf<\/td>\n\u00a0 \u25cf<\/td>\n\u00a0\u25cf<\/td>\nSphere<\/td>\n<\/tr>\n
Dot Blots<\/strong><\/a><\/td>\n<\/td>\n<\/td>\n\u00a0 \u25cf<\/td>\n<\/td>\nSphere<\/td>\n<\/tr>\n
Dark Field microscopy<\/strong><\/td>\n<\/td>\n<\/td>\n\u00a0 \u25cf<\/td>\n<\/td>\nSphere<\/td>\n<\/tr>\n
DLS<\/strong><\/td>\n\u00a0\u25cf<\/td>\n\u00a0\u25cf<\/td>\n\u00a0\u25cf<\/td>\n\u00a0\u25cf<\/td>\nSphere<\/td>\n<\/tr>\n
Flow Cytometry<\/strong><\/a><\/td>\n<\/td>\n<\/td>\n\u00a0\u25cf<\/td>\n\u00a0\u25cf<\/td>\nSphere<\/td>\n<\/tr>\n
Electron microscopy<\/strong><\/td>\n\u00a0\u25cf<\/td>\n\u00a0\u25cf<\/td>\n<\/td>\n<\/td>\nSphere<\/td>\n<\/tr>\n
Metal enhanced fluorescence<\/strong><\/td>\n<\/td>\n<\/td>\n\u00a0 \u25cf<\/td>\n<\/td>\nSphere\/flat surface<\/td>\n<\/tr>\n
SERS<\/strong><\/a><\/td>\n<\/td>\n<\/td>\n\u00a0 \u25cf<\/td>\n\u00a0\u25cf<\/td>\nNot spherical<\/td>\n<\/tr>\n
FRET quench<\/strong><\/td>\n\u00a0\u25cf<\/td>\n\u00a0\u25cf<\/td>\n\u00a0 \u25cf<\/td>\n<\/td>\nSphere<\/td>\n<\/tr>\n
Infrared phototermal therapy<\/strong><\/td>\n<\/td>\n\u00a0\u25cf<\/td>\n\u00a0 \u25cf<\/td>\n<\/td>\nRod\/hollow sphere<\/td>\n<\/tr>\n
Radiofrequency ablation<\/strong><\/td>\n<\/td>\n<\/td>\n\u00a0 \u25cf<\/td>\n<\/td>\nSphere<\/td>\n<\/tr>\n
Drug delivery<\/strong><\/td>\n\u00a0\u25cf<\/td>\n\u00a0\u25cf<\/td>\n\u00a0 \u25cf<\/td>\n<\/td>\nRod\/sphere<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u916a\u6c28\u9178\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | Tyrosinase Activity Assay Kit ATTOGENE\u8ca8\u865fE […]<\/p>\n","protected":false},"author":3,"featured_media":40470,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5384],"tags":[5402],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40469"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=40469"}],"version-history":[{"count":5,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40469\/revisions"}],"predecessor-version":[{"id":49184,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40469\/revisions\/49184"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/40470"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=40469"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=40469"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=40469"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}