{"id":40430,"date":"2023-03-23T13:40:10","date_gmt":"2023-03-23T05:40:10","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=40430"},"modified":"2023-03-28T11:34:39","modified_gmt":"2023-03-28T03:34:39","slug":"tyrosinase%e9%85%aa%e6%b0%a8%e9%85%b8%e9%85%b6%e6%b4%bb%e6%80%a7%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-abcam-ab252899-k742","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=40430","title":{"rendered":"Tyrosinase\u916a\u6c28\u9178\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2(\uc2dc\uc57d)| Abcam ab252899 (K742)"},"content":{"rendered":"

Tyrosinase\u916a\u6c28\u9178\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u5448\u8272\u6cd5\uff09 | <\/a>Abcam \u8ca8\u865fab252899<\/strong><\/span><\/p>\n

The first non-radioactive kit on the market that measure Tyrosinase activity in biological samples<\/em><\/p>\n

\u98df\u54c1\u5316\u5b78\/\u76ae\u819a\u76f8\u95dc\u7814\u7a76\u4e2d\u916a\u6c28\u9178\u9176\u6d3b\u6027\u7684\u5206\u6790<\/span><\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"<\/strong><\/p>\n

Tyrosinase\u916a\u6c28\u9178\u9176\uff08EC 1.14.18.1\uff09\u662f\u4e00\u7a2e\u9285\u7d50\u5408\u9176(copper-binding enzyme)\uff0c\u53ef\u5728\u5f9e\u7d30\u83cc(bacteria)\u3001\u771f\u83cc(fungi)\u3001\u5230\u54fa\u4e73\u52d5\u7269(mammals)\u7684\u5404\u7a2e\u7269\u7a2e\u4e2d\u8868\u9054\u3002\u5b83\u53c3\u8207\u9ed1\u8272\u7d20(melanin)\u5408\u6210\u9014\u5f91\u7684\u5169\u500b\u9023\u7e8c\u53cd\u61c9; \u9996\u5148\u662f\u55ae\u915a(monophenol)\u7684\u7fa5\u57fa\u5316(hydroxylation)\uff0c\u5176\u6b21\u662f\u9130\u4e8c\u915a(ortho-diphenol)\u8f49\u5316\u70ba\u918c(quinone)\u3002\u7136\u5f8c\u918c(quinone)\u9032\u884c\u4e00\u7cfb\u5217\u53cd\u61c9\uff0c\u5305\u62ec\u805a\u5408\u5f62\u6210\u9ed1\u8272\u7d20(melanin)\u3002Tyrosinase\u916a\u6c28\u9178\u9176\u5c0d\u8fb2\u696d\u7522\u696d\u975e\u5e38\u611f\u8208\u8da3\uff0c\u56e0\u70ba\u5b83\u6703\u5f15\u8d77\u6c34\u679c\uff0c\u852c\u83dc\u548c\u8611\u83c7\u7684\u8910\u8b8a(browning)\uff0c\u4ee5\u53ca\u5316\u599d\u54c1\u5de5\u696d\uff0c\u56e0\u70ba\u5b83\u6703\u5c0e\u81f4\u76ae\u819a\u8b8a\u9ed1(skin darkening)\u3002\u56e0\u6b64\uff0c\u916a\u6c28\u9178\u9176\u6291\u5236\u5291(tyrosinase inhibitors) \u7684\u958b\u767c\u548c\u7be9\u9078\u5c0d\u65bc\u8af8\u5982\u8272\u7d20\u6c89\u8457\u904e\u5ea6(hyperpigmentation)\u548c\u9ec3\u8910\u6591(melasma)\u7b49\u75c5\u75c7\u975e\u5e38\u6709\u7528\u3002\u916a\u6c28\u9178\u9176\u6d3b\u6027(Tyrosinase activity)\u5728\u9ed1\u7d20\u7624\u4e2d\u986f\u8457\u589e\u52a0\u3002\u56e0\u6b64\uff0c\u916a\u6c28\u9178\u9176\u6d3b\u6027\u7684\u6aa2\u6e2c\u53ef\u80fd\u6709\u5e0c\u671b\u4f5c\u70ba\u9ed1\u7d20\u7624\u7684\u7279\u7570\u6027\u8a3a\u65b7\u6e2c\u8a66\uff0c\u4e26\u4e14\u53ef\u7528\u65bc\u76e3\u6e2c\u60a3\u8005\u5c0d\u9ed1\u7d20\u7624(melanoma)\u6cbb\u7642\u7684\u53cd\u61c9\u3002 BioVision\u7684\u916a\u6c28\u9178\u9176\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2(Tyrosinase Activity Assay Kit, K742)\u662f\u4e00\u7a2e\u7c21\u55aeone-step\uff0c\u57fa\u65bc\u5e73\u677f\u7684\u6aa2\u6e2c\uff0c\u7528\u65bc\u6e2c\u91cf\u751f\u7269\u6a23\u54c1\u4e2d\u7684\u916a\u6c28\u9178\u9176\u6d3b\u6027\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0c\u916a\u6c28\u9178\u9176\u50ac\u5316\u915a\u985e\u5e95\u7269(phenolic substrate)\u8f49\u5316\u70ba\u918c\u4e2d\u9593\u9ad4(Quinone intermediate)\uff0c\u5176\u8207\u916a\u6c28\u9178\u589e\u5f37\u5291\u53cd\u61c9\u5f62\u6210\u9ad8\u5ea6\u7a69\u5b9a\u7684(chromophore)\uff0c\u5728510nm\u8655\u5177\u6709\u5438\u5149\u5ea6\u3002\u8a72\u6e2c\u5b9a\u53ef\u4ee5\u6aa2\u6e2c\u751f\u7269\u6a23\u54c1\u4e2d\u4f4e\u81f330\u03bcU\u7684\u916a\u6c28\u9178\u9176\u3002<\/p>\n

\"ELISA<\/a><\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

\u916a\u6c28\u9178\u9176\u7684\u9176\u52d5\u529b\u5b78<\/strong><\/span><\/p>\n

\n

\u916a\u6c28\u9178\u9176\u967d\u6027\u5c0d\u7167\u548c\u99ac\u9234\u85af\u88c2\u89e3\u7269\uff0816 \u00b5g \u86cb\u767d\u8cea\uff09\u7684\u9176\u52d5\u529b\u5b78\u3002<\/p>\n

\"\"<\/p>\n

\u672a\u8a98\u5c0e\u7684\u9ed1\u8272\u7d20\u7624\u7d30\u80de\u4e2d\u916a\u6c28\u9178\u9176\u6d3b\u6027\u7684\u9176\u52d5\u529b\u5b78<\/strong><\/span><\/div>\n
\n

\u5728 EMEM + 10% FBS\uff0815 \u00b5g \u86cb\u767d\u8cea\uff09\u4e2d\u57f9\u990a\u7684\u672a\u8a98\u5c0e\u9ed1\u8272\u7d20\u7624\u7d30\u80de\u4e2d\u916a\u6c28\u9178\u9176\u6d3b\u6027\u7684\u9176\u52d5\u529b\u5b78\uff0c\u4ee5\u53ca\u901a\u904e\u5728 EMEM + 0.5% FBS \u4e2d\u57f9\u990a 4 \u5929\u8a98\u5c0e\u589e\u52a0\u916a\u6c28\u9178\u9176\u6d3b\u6027\u7684\u9ed1\u8272\u7d20\u7624\u7d30\u80de\uff0c\u8f14\u4ee5 500 \u00b5M cAMP\uff0c100 \u00b5M PDE \u6291\u88fd\u5291 IBMX \u548c 100 \u00b5M Cu2+\uff0810 \u00b5g \u86cb\u767d\u8cea\uff09\u3002<\/p>\n

\"\"<\/p>\n

\u99ac\u9234\u85af\u88c2\u89e3\u7269\u548c\u9ed1\u8272\u7d20\u7624\u7d30\u80de\u4e2d\u7684\u916a\u6c28\u9178\u9176\u7279\u7570\u6027\u6d3b\u6027\u3002<\/strong><\/span><\/div>\n
\n

\u99ac\u9234\u85af\u88c2\u89e3\u7269\u548c\u9ed1\u8272\u7d20\u7624\u7d30\u80de\u4e2d\u7684\u916a\u6c28\u9178\u9176\u7279\u7570\u6027\u6d3b\u6027\u3002<\/p>\n<\/div>\n<\/div>\n<\/div>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nAbcam ab252899 (K742)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\n\u2022 Detection method- Absorbance (510 nm)
\n\u2022 Applications- Measurement of Tyrosinase Activity in various tissue\/cell lysates
\n– Analysis of Tyrosinase activity in food chemistry\/skin-related research<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (510 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nEukaryotes<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\u2022 Measurement of Tyrosinase Activity in various tissue\/cell lysates
\n\u2022 Analysis of Tyrosinase activity in food chemistry\/skin-related research<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 First non-radioactive assay in the market
\n\u2022 Simple, rapid & convenient
\n\u2022 The assay can detect Tyrosinase Activity less than 30 \u00b5U in biological samples.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Tyrosinase Assay Buffer
\n\u2022 Tyrosinase Substrate
\n\u2022 Tyrosinase Enhancer
\n\u2022 Chromophore Standard
\n\u2022 Tyrosinase Positive Control<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u00e7\u009b\u00b8\u00e9\u0097\u009c\u00e5\u0084\u0080\u00e5\u0099\u00a8\"<\/p>\n

\"CUBEE\u684c\u4e0a\u578b\u5c0f\u578b\u96e2\u5fc3\u6a5f\"<\/a>\"WPACO8000\"<\/a>\"MUPID-2<\/a>\"BIODROP-\u5be6\u9a57\u8a2d\u50993\"\"ELISA<\/a><\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

Tyrosinase\u916a\u6c28\u9178\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u5448\u8272\u6cd5\uff09 | Abcam \u8ca8\u865fab252899 The first […]<\/p>\n","protected":false},"author":3,"featured_media":37625,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261],"tags":[5408],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40430"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=40430"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40430\/revisions"}],"predecessor-version":[{"id":40573,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40430\/revisions\/40573"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/37625"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=40430"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=40430"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=40430"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}