{"id":40241,"date":"2023-03-17T14:36:42","date_gmt":"2023-03-17T06:36:42","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=40241"},"modified":"2023-03-28T11:39:57","modified_gmt":"2023-03-28T03:39:57","slug":"%e8%84%82%e6%b0%a7%e5%90%88%e9%85%b6%e6%b4%bb%e6%80%a7%e6%aa%a2%e6%b8%ac-lipoxygenase-activity-assay-kit-abcam%e8%b2%a8%e8%99%9fab241038-k978-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=40241","title":{"rendered":"\u8102\u6c27\u5408\u9176\u6d3b\u6027\u6aa2\u6e2c(\uc2dc\uc57d) | Lipoxygenase Activity Assay Kit-Abcam\u8ca8\u865fab241038 (k978-100)"},"content":{"rendered":"

\u8102\u6c27\u5408\u9176\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0|\u00a0Abcam\u8ca8\u865f<\/a>\u00a0ab241038 (K978-100)<\/span><\/strong><\/span><\/p>\n

Lipoxygenase Activity Assay Kit (Fluorometric)<\/em><\/strong><\/span><\/p>\n

0.004 mU \/ mg\u86cb\u767d\u8cea<\/span>\"\"<\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>Lipoxygenases\u8102\u6c27\u5408\u9176\u662f\u975e\u8840\u7d05\u7d20\u9435(heme iron)\u7684\u96d9\u52a0\u6c27\u9176(dioxygenases)\u3002\u9019\u4e9b\u9176\u5c07\u4e0d\u98fd\u548c\u8102\u80aa\u9178\u8f49\u5316\u70ba\u74b0\u6c27\u5316\u7269;\u4f8b\u5982\uff0c\u5f9e\u82b1\u751f\u56db\u70ef\u9178(arachidonic acid)\u5408\u6210\u767d\u4e09\u70ef(leukotrienes)\u3002\u5b83\u5011\u5728\u7d30\u80de\u589e\u6b96(cell proliferation)\uff0c\u5206\u5316(differentiation)\u548c\u708e\u75c7(inflammation)\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528;\u4e26\u4e14\u5df2\u7d93\u6d89\u53ca\u708e\u75c7(inflammation)\u548c\u904e\u5ea6\u589e\u6b96(hyper proliferation)\u4ecb\u5c0e\u7684\u75be\u75c5\uff0c\u5982\u54ee\u5598(asthma)\uff0c\u985e\u98a8\u6fd5\u6027\u95dc\u7bc0\u708e(rheumatoid arthritis)\u548c\u764c\u75c7\u3002\u5728BioVision\u7684\u8102\u6c27\u5408\u9176\uff08Lipoxygenase , LOX\uff09\u6d3b\u6027\u6e2c\u5b9a\u4e2d\uff0cLipoxygenase\u8102\u6c27\u5408\u9176\u5c07LOX substrate\u8f49\u5316\u70ba\u8207\u7522\u751f\u87a2\u5149\u7522\u7269\u7684\u63a2\u91dd\u53cd\u61c9\u7684\u4e2d\u9593\u9ad4\u3002\u87a2\u5149\u4fe1\u865f\u7684\u589e\u52a0\u53ef\u4ee5\u5728Ex \/ Em 500 \/ 536nm\u8655\u8a18\u9304\uff0c\u4e26\u4e14\u8207LOX\u6d3b\u6027\u6210\u6b63\u6bd4\u3002\u8a72\u8a66\u5291\u76d2\u5305\u62ec5-\u8102\u6c27\u5408\u9176\uff085-lipoxygenase enzyme , LOX\u9176\uff09\u4f5c\u70ba\u967d\u6027\u5c0d\u7167\u3002\u9084\u5305\u62ec\u5b8c\u5168\u6291\u5236\u8102\u6c27\u5408\u9176\u6d3b\u6027\u7684lipoxygenase inhibitor\u8102\u6c27\u5408\u9176\u6291\u5236\u5291\uff0c\u4ee5\u8a08\u7b97\u751f\u7269\u6a23\u54c1\u4e2dLOX\u7684\u6bd4\u6d3b\u6027\u3002\u8a72\u8a66\u5291\u76d2\u53ef\u6aa2\u6e2c\u4f4e\u81f30.004 mU \/ mg\u86cb\u767d\u8cea\u3002<\/p>\n

\"\"<\/p>\n

\u6c27\u5316\u63a2\u91dd\u6a19\u6e96\u66f2\u7dda\u3002<\/strong><\/span><\/div>\n
\u6c27\u5316\u63a2\u91dd\u6a19\u6e96\u66f2\u7dda\u3002<\/div>\n
\"\"<\/div>\n
\n
\u967d\u6027\u5c0d\u7167\u548c MCF-7 \u88c2\u89e3\u7269\u4e2d\u7684\u8102\u6c27\u5408\u9176\u53cd\u61c9\u3002<\/strong><\/span><\/div>\n
\n

\u967d\u6027\u5c0d\u7167\u548c MCF-7 \u88c2\u89e3\u7269\uff0810 \u00b5g \u86cb\u767d\u8cea\uff09\u4e2d\u7684\u8102\u6c27\u5408\u9176\u53cd\u61c9\u3002<\/p>\n

\"\"<\/p>\n

\u967d\u6027\u5c0d\u7167\u548c MCF-7 \u88c2\u89e3\u7269\u4e2d\u7684 LOX \u6d3b\u6027\u3002<\/strong><\/span><\/div>\n
\u967d\u6027\u5c0d\u7167\u548c MCF-7 \u88c2\u89e3\u7269\u4e2d\u7684 LOX \u6d3b\u6027\u3002\u6309\u7167\u8a66\u5291\u76d2\u65b9\u6848\u9032\u884c\u6e2c\u5b9a\u3002<\/div>\n
<\/div>\n<\/div>\n<\/div>\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK978-100\u00a0\u00a0\u00a0(Abcam ab241038)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFluorescence (Ex\/Em 500\/536 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nMeasurement of LOX activity in various tissues\/cell extracts, biological fluids, and purified proteins
\n– Determination of LOX activity associated with pathological conditions
\n-Characterization of activity of purified LOX enzymes<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes only ~40 min
\n\u2022 Fast and convenient<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 LOX Assay Buffer
\n\u2022 Oxidized Probe Standard (100 \u00b5M)
\n\u2022 LOX Probe
\n\u2022 LOX Substrate
\n\u2022 LOX Inhibitor
\n\u2022 LOX Enzyme<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u8102\u6c27\u5408\u9176\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0|\u00a0Abcam\u8ca8\u865f\u00a0ab241038 (K978-100) Lipoxygenase […]<\/p>\n","protected":false},"author":3,"featured_media":37625,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261],"tags":[5425],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40241"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=40241"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40241\/revisions"}],"predecessor-version":[{"id":40578,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40241\/revisions\/40578"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/37625"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=40241"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=40241"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=40241"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}