{"id":40200,"date":"2023-03-16T18:40:02","date_gmt":"2023-03-16T10:40:02","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=40200"},"modified":"2023-03-28T11:40:47","modified_gmt":"2023-03-28T03:40:47","slug":"%e9%b9%bc%e6%80%a7%e9%9e%98%e7%a3%b7%e8%84%82%e9%85%b6%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-ab241039-abcam%e8%b2%a8%e8%99%9fab241039-k987","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=40200","title":{"rendered":"\u9e7c\u6027\u9798\u78f7\u8102\u9176\u6aa2\u6e2c\u8a66\u5291\u76d2 (ab241039)| ABCAM\u8ca8\u865fab241039 (K987)"},"content":{"rendered":"

\u9e7c\u6027\u9798\u78f7\u8102\u9176\u6aa2\u6e2c\u8a66\u5291\u76d2 (ab241039)| ABCAM<\/strong>\u8ca8\u865fab241039<\/a><\/strong><\/a>\u00a0(K987)<\/span><\/p>\n

Alkaline Sphingomyelinase Assay Kit (ab241039)<\/p>\n

I. \u9798\u78f7\u8102\u9176\uff08Sphingomyelinase, SMase\uff0cE.C. 3.1.4.12\uff09\u88c2\u89e3\u9798\u78f7\u8102(sphingomyelin )\u7522\u751f\u78f7\u9178\u81bd\u9e7c(phosphocholine)\u548cceramide\u3002\u9019\u5169\u7a2e\u5206\u5b50\u90fd\u53ef\u4ee5\u5145\u7576\u7b2c\u4e8c\u4fe1\u4f7f\u4e26\u8abf\u7bc0\u4e0b\u6e38\u4e8b\u4ef6\uff0c\u5305\u62ec\u7d30\u80de\u51cb\u4ea1(apoptosis)\u3001\u7d30\u80de\u589e\u6b96(cell proliferation)\u548csterol homeostasis\u7a69\u614b\u3002\u9798\u78f7\u8102\u9176\u5bb6\u65cf(\u00a0sphingomyelinase family )\u5305\u62ec\u5728\u6eb6\u9176\u9ad4\u4e2d\u767c\u73fe\u4e26\u5206\u6ccc\u7684\u9178\u6027\u9798\u78f7\u8102\u9176(acid sphingomyelinase)\u3001\u5728\u8166\u4e2d\u5bcc\u96c6\u7684\u4e2d\u6027\u9798\u78f7\u8102\u9176\u548c\u5728\u8178\u9053\u4e2d\u8d77\u4f5c\u7528\u7684\u9e7c\u6027\u9798\u78f7\u8102\u9176 (AlkSMase)\u3002\u8207\u4e2d\u6027\u54c1\u7a2e (nSMase) \u4e0d\u540c\uff0cAlkSMase \u5728\u8f03\u9ad8 pH \u503c\u4e0b\u8868\u73fe\u51fa\u6700\u4f73\u6d3b\u6027\uff0c\u4e26\u4e14\u9176\u4fc3\u6d3b\u6027\u4e0d\u9700\u8981 Mg2+\u3002 AlkSMase\uff0c\u4e5f\u7a31\u70ba NPP7\uff0c\u56e0\u70ba\u5b83\u8207\u6838\u82f7\u9178\u7126\u78f7\u9178\u9176(Nucleotide Pyrophosphatase)\/\u78f7\u9178\u4e8c\u916f\u9176\u9176(Phosphodiesterase)\u5bb6\u65cf\u5177\u6709\u540c\u6e90\u6027\uff0c\u8207\u9810\u9632\u816b\u7624\u5f62\u6210(tumor formation)\u6709\u95dc\u3002\u5b83\u7684\u6d3b\u6027\u5728\u67d0\u4e9b\u8178\u764c\u4ee5\u53ca\u6162\u6027\u7d50\u8178\u708e\u7684\u60c5\u6cc1\u4e0b\u6703\u964d\u4f4e\u3002\u9e7c\u6027\u9798\u78f7\u8102\u9176(Alkaline Sphingomyelinase )\u4e5f\u53ef\u80fd\u5728\u81bd\u56fa\u9187\u5438\u6536\u4e2d\u767c\u63ee\u4f5c\u7528\u3002 BioVision \u7684\u9e7c\u6027\u9798\u78f7\u8102\u9176\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u3001\u9ad8\u901a\u91cf\u9069\u61c9\u6027\u5f37\u7684\u65b9\u6cd5\u4f86\u9451\u5b9a\u548c\u91cf\u5316\u5404\u7a2e\u6a23\u54c1\u4e2d\u7684\u9e7c\u6027\u9798\u78f7\u8102\u9176(Alkaline Sphingomyelinase )\u6d3b\u6027\uff0c\u800c\u4e0d\u53d7\u4e2d\u6027\u6216\u9178\u6027\u9798\u78f7\u8102\u9176(Alkaline Sphingomyelinase )\u7684\u5f71\u97ff\u3002\u5728\u8207\u5e95\u7269\u5b75\u80b2\u5f8c\uff0c\u6c34\u89e3\u7522\u751f\u4e00\u7a2e\u4e2d\u9593\u9ad4\uff0c\u8a72\u4e2d\u9593\u9ad4\u8207\u63a2\u91dd\u53cd\u61c9\uff0c\u7522\u751f\u6bd4\u8272\u4fe1\u865f\u3002\u8a72\u6e2c\u5b9a\u6cd5\u53ef\u6aa2\u6e2c\u4f4e\u81f3 0.5 \u00b5U \u7684\u9e7c\u6027\u9798\u78f7\u8102\u9176(Alkaline Sphingomyelinase )\u6d3b\u6027\u3002<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

NPP7 \u7684\u9e7c\u6027\u9798\u78f7\u8102\u9176\u6d3b\u6027\u3002<\/strong><\/span><\/div>\n
NPP7 \u7684\u9e7c\u6027\u9798\u78f7\u8102\u9176\u6d3b\u6027\u3002\u5141\u8a31\u6c34\u89e3\u9032\u884c1\u5c0f\u6642\u3002\u4e2d\u6027\u9798\u78f7\u8102\u9176\uff08nSMase\uff0c\u7d2b\u8272\u689d\uff09\u5728\u76f8\u540c\u7684\u5b75\u80b2\u6642\u9593\u5f8c\u986f\u793a\u51fa\u6700\u5c0f\u7684\u6d3b\u6027\u3002<\/div>\n
\"\"<\/div>\n
\n
\u8178\u7d44\u7e54\u88c2\u89e3\u7269\u7684\u6d3b\u6027\u6e2c\u5b9a\u3002<\/strong><\/span><\/div>\n
\n

\u8178\u7d44\u7e54\u88c2\u89e3\u7269\u7684\u6d3b\u6027\u6e2c\u5b9a\u3002\u5c0d\u65bc\u8a72\u5be6\u9a57\uff0c\u4f7f\u7528 100 mg \u5927\u9f20\u8178\uff0c\u9075\u5faa\u9e7c\u6027\u9798\u78f7\u8102\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u65b9\u6848\u548c 2X \u86cb\u767d\u9176\u6291\u88fd\u5291\u3002\u5c0d\u88c2\u89e3\u7269\u9032\u884c\u4e86\u6e2c\u5b9a\uff0c\u6bd4\u6d3b\u6027\u78ba\u5b9a\u70ba 0.016 nmol\/min\/\u00b5g \u88c2\u89e3\u7269\u3002<\/p>\n<\/div>\n<\/div>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

\u6297 BRAF\uff08\u7a81\u8b8a\u7684 V600E\uff09\u6297\u9ad4<\/a>\u00a0|\u00a0\u6297 HMGB1 \u6297\u9ad4<\/a>\u00a0|\u00a0\u6297 EGFR \u6297\u9ad4 [EP38Y]\u00a0<\/a>\u00a0|\u00a0\u6297 NF-kB p65 \u6297\u9ad4\u00a0<\/a>\u00a0|\u00a0\u6297 Ki67 \u6297\u9ad4<\/a>\u00a0|\u00a0IV \u578b\u81a0\u539f\u86cb\u767d\u6297\u9ad4<\/a><\/span><\/strong><\/p>\n

\"\"<\/p>\n

http:\/\/www.abcam.com\/<\/p>\n

Abcam\u8b58\u5225\u3001\u958b\u767c\u548c\u63d0\u4f9b\u9ad8\u8cea\u91cf\u7684\u751f\u7269\u8a66\u5291\u548c\u5de5\u5177\uff0c\u5b83\u5011\u5728\u5305\u62ec\u85e5\u7269\u767c\u73fe\u3001\u8a3a\u65b7\u548c\u57fa\u790e\u7814\u7a76\u5728\u5167\u7684\u5ee3\u6cdb\u9818\u57df\u548c\u61c9\u7528\u4e2d\u5fc5\u4e0d\u53ef\u5c11\u3002Abcam \u5c08\u6ce8\u5404\u7a2e\u7814\u7a76\u3001\u8a3a\u65b7\u53ca\u6cbb\u7642\u7528\u4e4b\u55ae\u682a\u6297\u9ad4\u7684\u958b\u767c\uff0c\u64c1\u6709 RabMAb\u00ae \u5154\u5b50\u55ae\u682a\u6297\u9ad4\u751f\u7522\u6280\u8853\u5c08\u5229\u4e4b\u539f\u59cb\u5ee0\u5546\uff0c\u73fe\u4eca\u70ba Abcam \u7684\u5408\u4f5c\u5925\u4f34\u3002\u8207\u5c0f\u9f20\u55ae\u682a\u6297\u9ad4\u76f8\u6bd4\uff0c\u5154\u5b50\u55ae\u682a\u6297\u9ad4\u5177\u6709\u66f4\u512a\u826f\u7684\u6297\u539f\u8fa8\u8b58\u529b\u3001\u66f4\u5f37\u7684\u89aa\u548c\u529b\u8207\u66f4\u9ad8\u7684\u5c08\u4e00\u5ea6\uff0c\u662f\u60a8\u5be6\u9a57\u7528\u55ae\u682a\u6297\u9ad4\u7684\u6700\u4f73\u9078\u64c7\u3002<\/p>\n

Abcam\u70ba\u4e16\u754c\u4e0a\u4e09\u5206\u4e4b\u4e8c\u7684\u751f\u547d\u79d1\u5b78\u5bb6\u63d0\u4f9b\u8d85\u904e 110,000 \u7a2e\u7522\u54c1\uff0c\u7528\u65bc\u7814\u7a76\u86cb\u767d\u8cea\u9776\u6a19 – \u6db5\u84cb\u6297\u9ad4\u3001\u86cb\u767d\u8cea\u3001\u88c2\u89e3\u7269\u3001\u751f\u7269\u5316\u5b78\u88fd\u54c1\u3001\u8a66\u5291\u76d2\u548c\u6aa2\u6e2c\u8a66\u5291\u76d2\u3002 ABCAM\u5728\u5168\u7403\u7684\u5c08\u696d\u8a2d\u65bd\u4e2d\u751f\u7522\u81ea\u5df1\u7684\u7522\u54c1\uff0c\u4e26\u5f9e 400 \u591a\u500b\u63a1\u8cfc\u5546\u8655\u63a1\u8cfc\u5925\u4f34\u3002\u8207\u751f\u547d\u79d1\u5b78\u884c\u696d\u5408\u4f5c\uff0c\u4fc3\u9032\u672a\u4f86\u85e5\u7269\u7684\u958b\u767c\u3002<\/span><\/p>\n

\u7279\u8272\u7522\u54c1\uff1a9\u842c\u591a\u7a2e\u9ad8\u8cea\u91cf\u4e00\u6297<\/a>\u30012800\u591a\u7a2e\u4e8c\u6297<\/a>\u300190\u5206\u9418\u5feb\u901f\u578b\u53ca\u50b3\u7d71ELISA \u548c\u5339\u914d\u7684\u6297\u9ad4\u5c0d<\/a>\u3001\u6572\u9664\u7d30\u80de\u7cfb\u548c\u88c2\u89e3\u7269<\/a>\u3001\u6fc0\u52d5\u5291\u3001\u62ee\u6297\u5291\u3001\u6fc0\u6d3b\u5291\u548c\u6291\u5236\u5291<\/a>\uff0cDNA\u7532\u57fa\u5316\u8207\u7d44\u86cb\u767d\u4fee\u98fe\u8a66\u5291\uff0c\u7dda\u7c92\u9ad4\u7814\u7a76\u8a66\u5291\uff0c\u7d30\u80de\u6d3b\u6027\u53ca\u51cb\u4ea1\u7b49\u529f\u80fd\u6aa2\u6e2c\u8a66\u5291\u76d2\uff0c\u4eba\u6e90\u5316\u7d30\u80de\u56e0\u5b50\u53ca\u91cd\u7d44\u86cb\u767d\u7b49\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u9e7c\u6027\u9798\u78f7\u8102\u9176\u6aa2\u6e2c\u8a66\u5291\u76d2 (ab241039)| ABCAM\u8ca8\u865fab241039\u00a0(K987) Alkaline […]<\/p>\n","protected":false},"author":3,"featured_media":37625,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261],"tags":[5429],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40200"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=40200"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40200\/revisions"}],"predecessor-version":[{"id":40201,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40200\/revisions\/40201"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/37625"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=40200"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=40200"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=40200"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}