{"id":40129,"date":"2023-03-15T17:54:45","date_gmt":"2023-03-15T09:54:45","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=40129"},"modified":"2023-03-28T11:42:56","modified_gmt":"2023-03-28T03:42:56","slug":"caspase-8-ietd-r110-%e8%9e%a2%e5%85%89%e5%92%8c%e6%af%94%e8%89%b2%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-biotium-%e8%b2%a8%e8%99%9f30008-2","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=40129","title":{"rendered":"Caspase-8 IETD-R110 \u87a2\u5149\u548c\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | Biotium \u8ca8\u865f30008-2"},"content":{"rendered":"<p><strong><span style=\"color: #33cccc; font-size: 12pt;\"><a style=\"color: #33cccc;\" href=\"http:\/\/biopioneer.com.tw\/?p=40129\">Caspase-8 IETD-R110 \u87a2\u5149\u548c\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | Biotium \u8ca8\u865f30008-2<\/a><\/span><\/strong><\/p>\n<p><span style=\"color: #ff9900;\">Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit<\/span><\/p>\n<p><img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/03\/Caspase-8-IETD-R110-%E8%9E%A2%E5%85%89%E5%92%8C%E6%AF%94%E8%89%B2%E6%B8%AC%E5%AE%9A%E8%A9%A6%E5%8A%91%E7%9B%92Biotium-%E8%B2%A8%E8%99%9F30008-2-%E5%8E%9F%E7%90%86.jpg\" alt=\"\" \/><\/p>\n<p><span style=\"color: #800000; font-size: 12pt;\">Figure 1. Two-step cleavage of R110-based substrates by peptidases to release green fluorescent R110 dye. R represents the peptide substrate (DEVD for Caspase-3 substrate)<\/span><\/p>\n<p>Caspase-8 \u662f CD95\/Fas \u51cb\u4ea1\u901a\u8def\u4e2d\u6700\u4e0a\u6e38\u7684\u534a\u80f1\u5929\u51ac\u9176\uff0c\u88ab CD95\/Fas \u548c TNF \u7684\u4fe1\u865f\u901a\u8def\u6fc0\u6d3b\u3002\u87a2\u5149\u548c\u986f\u8272\u5e95\u7269 (Ac-IETD)2-R110 \u5305\u542b\u5169\u500b IETD \u56db\u80bd\uff0c\u4e26\u5728\u5169\u500b\u9023\u7e8c\u6b65\u9a5f\u4e2d\u88ab caspase-8 \u5b8c\u5168\u6c34\u89e3\u3002\u7b2c\u4e00\u500b IETD \u80bd\u7684\u88c2\u89e3\u7522\u751f\u55ae\u80bd Ac-IETD-R110 \u4e2d\u9593\u9ad4\uff0c\u5176\u5438\u6536\u548c\u767c\u5c04\u6ce2\u9577\u8207 R110\uff08\u7f85\u4e39\u660e 110\uff09\uff08Ex\/Em= 496\/520 nm\uff09\u76f8\u4f3c\uff0c\u4f46\u53ea\u6709\u7d04 10%\u5f8c\u8005\u7684\u87a2\u5149\u3002\u7b2c\u4e8c\u500b IETD \u80bd\u7684\u6c34\u89e3\u91cb\u653e\u67d3\u6599 R110\uff0c\u5c0e\u81f4\u87a2\u5149\u986f\u8457\u589e\u52a0\u3002\u96d6\u7136\u6700\u7d42\u7522\u54c1\u7684\u87a2\u5149\u6aa2\u6e2c\u7531\u65bc\u9748\u654f\u5ea6\u9ad8\u800c\u6210\u70ba\u9996\u9078\uff0c\u4f46\u4e5f\u53ef\u4ee5\u901a\u904e\u5438\u5149\u5ea6\u9032\u884c\u6aa2\u6e2c\u3002\u4e8b\u5be6\u4e0a\uff0cR110 \u7684\u6d88\u5149\u4fc2\u6578\u6bd4\u5c0d\u785d\u57fa\u82ef\u80fa (pNA) \u9ad8 10 \u500d\uff0c\u5c0d\u785d\u57fa\u82ef\u80fa (pNA) \u662f\u4e00\u7a2e\u5e38\u7528\u65bc\u986f\u8272\u5e95\u7269\u7684\u67d3\u6599\uff0c\u9019\u4f7f\u5f97\u57fa\u65bc R110 \u7684\u5e95\u7269\u6bd4\u57fa\u65bc pNA \u7684\u5e95\u7269\u66f4\u52a0\u654f\u611f\uff0c\u5373\u4f7f\u901a\u904e\u6bd4\u8272\u6aa2\u6e2c\u4e5f\u662f\u5982\u6b64\u3002\u8a72\u6aa2\u6e2c\u8a66\u5291\u76d2\u5305\u62ec Ac-IETD-CHO\uff0c\u5b83\u662f\u4e00\u7a2e caspase-8 \u6291\u88fd\u5291\uff0c\u53ef\u7528\u4f5c\u9670\u6027\u5c0d\u7167\u3002\u8a66\u5291\u76d2\u4e2d\u9084\u63d0\u4f9b\u4e86 R110\uff0c\u7528\u65bc\u751f\u6210\u6a19\u6e96\u66f2\u7dda\uff0c\u53ef\u7528\u65bc\u5b9a\u91cf caspase-8 \u6d3b\u6027\u3002<\/p>\n<p>\u96d6\u7136\u8207\u5176\u4ed6\u5e95\u7269\u5e8f\u5217\u76f8\u6bd4\uff0ccaspase-8 \u512a\u5148\u5207\u5272\u5171\u6709\u5e8f\u5217 IETD\uff0c\u4f46\u5176\u4ed6 caspase\uff08\u5982 caspase-3\uff09\u4e5f\u53ef\u4ee5\u6709\u6548\u5207\u5272 IETD\u3002\u91cd\u758a\u7684\u534a\u80f1\u5929\u51ac\u9176\u5e95\u7269\u8b58\u5225\u9650\u5236\u4e86\u534a\u80f1\u5929\u51ac\u9176\u5e95\u7269\u80bd\u5728\u5340\u5206\u7d30\u80de\u88c2\u89e3\u7269\u4e2d\u4e0d\u540c\u534a\u80f1\u5929\u51ac\u9176\u6d3b\u6027\u65b9\u9762\u7684\u7528\u9014\u3002Caspase-8 \u87a2\u5149\u548c\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u500b\u7c21\u55ae\u7684\u6e2c\u5b9a\u4fc2\u7d71\uff0c\u53ef\u901a\u904e\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u4e2d\u7684\u87a2\u5149\u6216\u5438\u5149\u5ea6\u5feb\u901f\u3001\u9ad8\u5ea6\u9748\u654f\u5730\u6aa2\u6e2c caspase-8 \u6d3b\u6027\u3002 Biotium \u9084\u63d0\u4f9b Caspase-IETD-R110 \u87a2\u5149 HTS \u6aa2\u6e2c\u8a66\u5291\u76d2 (30012)\uff0c\u9019\u662f\u4e00\u7a2e\u7528\u65bc\u9ad8\u901a\u91cf\u7be9\u9078 (HTS) \u7684\u55ae\u6b65\u87a2\u5149\u6aa2\u6e2c\u3002<\/p>\n<p><a href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/03\/Caspase-8-IETD-R110-\u87a2\u5149\u548c\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2-Biotium-\u8ca8\u865f30008-2.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/01\/%E7%B6%B2%E7%AB%99%E8%A6%8F%E6%A0%BC%E5%B0%8F%E5%9C%96_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-11-300x97.jpg\" alt=\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df 1_\u5de5\u4f5c\u5340\u57df 1\" width=\"149\" height=\"48\" \/><\/a><\/p>\n<div class=\"content-column one_half\">\n<div>\n<h3 class=\"style5 style11\"><span style=\"font-size: 12pt; color: #800000;\">Features<\/span><\/h3>\n<ul>\n<li class=\"style5 style11\">Caspase-8 activity measured with substrate (Ac-IETD)2-R110<\/li>\n<li class=\"style5 style11\">Compatible with both fluorometric and colorimetric detection systems<\/li>\n<li>Fast enzyme kinetics<\/li>\n<li>Enzymatic reaction forms yellow colored and green fluorescent R110 product<\/li>\n<li>Endpoint assay requires cell lysis<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<div class=\"content-column one_half last_column\">\n<h3><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\">Kit Components<\/span><\/strong><\/span><\/h3>\n<ul>\n<li>Cell lysis buffer<\/li>\n<li>Assay buffer<\/li>\n<li>Enzyme substrate (Ac-IETD)2-R110<\/li>\n<li>Enzyme inhibitor Ac-IETD-CHO<\/li>\n<li>R110<\/li>\n<\/ul>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/03\/Caspase-8-IETD-R110-%E8%9E%A2%E5%85%89%E5%92%8C%E6%AF%94%E8%89%B2%E6%B8%AC%E5%AE%9A%E8%A9%A6%E5%8A%91%E7%9B%92-Biotium-%E8%B2%A8%E8%99%9F30008-2.jpg\" alt=\"\" width=\"497\" height=\"269\" \/><\/p>\n<\/div>\n<p>1. Neurochem Res (2009) Aug;34(8):1410-6.\u00a0<a href=\"https:\/\/idp.springer.com\/authorize?response_type=cookie&amp;client_id=springerlink&amp;redirect_uri=https:\/\/link.springer.com\/article\/10.1007\/s11064-009-9927-6\">doi: 10.1007\/s11064-009-9927-6<\/a><br \/>\n2. J Psychiatr Res (2009) May;43(8):754-62\u00a0<a href=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S0022395608002318?via%3Dihub\">doi: 10.1016\/j.jpsychires.2008.10.002.<\/a><br \/>\n3. Eur J Pharmacol (2009) May 21;610(1-3):99-105\u00a0<a href=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S0014299909002623\">doi: 10.1016\/j.ejphar.2009.03.046<\/a><br \/>\n4. Fundam Clin Pharmacol (2010) Aug;24(4):445-50\u00a0<a href=\"https:\/\/onlinelibrary.wiley.com\/doi\/abs\/10.1111\/j.1472-8206.2009.00789.x\">doi: 10.1111\/j.1472-8206.2009.00789.x<\/a><br \/>\n5. Asian Pac J Cancer Prev (2018) 19(4): 917\u2013922\u00a0<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6031786\/\">doi: 10.22034\/APJCP.2018.19.4.917<\/a><\/p>\n<p><img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/03\/Caspase-8-IETD-R110-%E8%9E%A2%E5%85%89%E5%92%8C%E6%AF%94%E8%89%B2%E6%B8%AC%E5%AE%9A%E8%A9%A6%E5%8A%91%E7%9B%92.jpg\" alt=\"\" \/><\/p>\n<p><strong><span style=\"font-size: 12pt; color: #800000;\">Biotium \u63d0\u4f9b\u7bc4\u570d\u5ee3\u6cdb\u7684\u87a2\u5149\u63a2\u91dd\u7528\u65bc\u7814\u7a76\u51cb\u4ea1\u7d30\u80de(Apotosis)<\/span><\/strong><\/p>\n<p><img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/03\/biotium.jpg\" alt=\"\" \/><\/p>\n<p>The\u00a0<a href=\"https:\/\/biotium.com\/product\/nucview-caspase-3-substrates\/\" target=\"_blank\" rel=\"noopener noreferrer\">NucView\u00ae Caspase-3 Substrate<\/a>\u00a0comes in 3 colors, and measures caspase activity in real time, in live cells.\u00a0<a href=\"https:\/\/biotium.com\/product\/mitoview-dyes\/\" target=\"_blank\" rel=\"noopener noreferrer\">MitoView\u2122 633<\/a>\u00a0can measure the decrease in mitochondria membrane potential that occurs in early apoptosis.\u00a0<a href=\"https:\/\/biotium.com\/product\/annexin-v-conjugates\/\" target=\"_blank\" rel=\"noopener noreferrer\">CF\u00ae Dye Annexin V<\/a>\u00a0conjugates come in many color options, and label PS on the membranes of late apoptotic cells. We also offer kits containing combinations of these probes.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Caspase-8 IETD-R110 \u87a2\u5149\u548c\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | Biotium \u8ca8\u865f30008-2 Casp [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":40130,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5378],"tags":[5438],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40129"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=40129"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40129\/revisions"}],"predecessor-version":[{"id":40132,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40129\/revisions\/40132"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/40130"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=40129"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=40129"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=40129"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}