{"id":40025,"date":"2023-03-13T17:19:23","date_gmt":"2023-03-13T09:19:23","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=40025"},"modified":"2023-03-28T11:45:08","modified_gmt":"2023-03-28T03:45:08","slug":"cox2%e6%8a%97%e9%ab%94-cox2-antibody%e8%b2%a8%e8%99%9fir109-446-ireal-biotechnology-%e8%89%be%e9%8b%ad%e7%94%9f%e6%8a%80","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=40025","title":{"rendered":"COX2\u6297\u9ad4 | COX2 antibody\u8ca8\u865fIR109-446 (IReal Biotechnology \u827e\u92ed\u751f\u6280)"},"content":{"rendered":"

IHC\/WB\/IF\/IP\u7814\u7a76\u5de5\u5177-Inflammatory\/ Inflammasome Antibodies<\/strong><\/span>–IReal Biotechnology<\/span><\/span><\/p>\n

\u74b0\u6c27\u5408\u9176 1 (Cyclooxygenase1, Cox1) \u548c\u74b0\u6c27\u5408\u9176 2 (cyclooxygenase2\u00a0Cox2) \u662f\u5728\u4eba\u985e\u4e2d\u5177\u6709 60% \u540c\u6e90\u6027\u7684\u5bb6\u65cf\u6210\u54e1\uff0c\u5b83\u5011\u50ac\u5316\u82b1\u751f\u56db\u70ef\u9178\u7522\u751f\u524d\u5217\u817a\u7d20 (arachidonic acid)\u3002\u96d6\u7136 Cox1 \u8868\u9054\u5728\u5927\u591a\u6578\u7d44\u7e54\u4e2d\u662f\u7d44\u6210\u578b\u7684\uff0c\u4f46 Cox2 \u8868\u9054\u662f\u7531\u8102\u591a\u91a3 (\u00a0lipopolysaccharide, LPS) \u548c\u80bd\u805a\u91a3 (\u00a0peptidoglycan, PGN) \u8a98\u5c0e\u7684 \u3002 PGN \u6fc0\u6d3b Ras\uff0c\u5c0e\u81f4 Raf \u5728 Ser338 \u4f4d\u9ede\u548c Erk1\/2 \u5728 Tyr204 \u4f4d\u9ede\u7684\u78f7\u9178\u5316(phosphorylation )\u3002 MAP \u6fc0\u9176\u4fe1\u865f(\u00a0MAP kinase signaling)\u50b3\u5c0e\u7684\u6fc0\u6d3b\u5c0e\u81f4 IKK\u03b1\/\u03b2 \u7684\u5f8c\u7e8c\u6fc0\u6d3b\u3001I\u03baB\u03b1 \u5728 Ser32\/36 \u4f4d\u9ede\u7684\u78f7\u9178\u5316\u548c NF-\u03baB \u6fc0\u6d3b\u3002\u6700\u5f8c\uff0c\u8f49\u9304\u56e0\u5b50 NF-\u03baB \u7684\u6fc0\u6d3b\u8ca0\u8cac\u8a98\u5c0e Cox2 \u8868\u9054 \u3002\u7814\u7a76\u4eba\u54e1\u8868\u660e\uff0cLPS \u548c PGN \u6703\u8a98\u767c\u95dc\u7bc0\u708e(arthritis )\u548c\u7d30\u83cc\u611f\u67d3(bacterial infections)\u7684\u81e8\u5e8a\u8868\u73fe\uff0c\u4f8b\u5982\u708e\u75c7(inflammation)\u3001\u767c\u71b1(\u00a0fever)\u548c\u611f\u67d3\u6027\u4f11\u514b(septic shock) \u3002\u7814\u7a76\u8868\u660e\uff0cCox1 \u548c Cox2 \u9084\u53ef\u80fd\u901a\u904e\u589e\u5f37 \u03b3-\u5206\u6ccc\u9176\u6d3b\u6027\u548c \u03b2-\u6fb1\u7c89\u6a23\u86cb\u767d(\u03b2-amyloid )\u751f\u6210\u800c\u5728\u963f\u723e\u8328\u6d77\u9ed8\u75c5\u7684\u795e\u7d93\u75c5\u7406\u5b78(neuropathology)\u4e2d\u767c\u63ee\u4f5c\u7528 \u3002<\/p>\n

\"\"<\/p>\n

\"HOT\"<\/a>COX2\u00a0\u6297\u9ad4 | COX2 antibody\u8ca8\u865fIR109-446 \u00a0(IReal Biotechnology \u827e\u92ed\u751f\u6280)<\/a><\/span><\/span><\/strong><\/span><\/p>\n

COX2 antibody (IReal Biotechnology \u827e\u92ed\u751f\u6280,IR109-446)<\/p>\n

iReal \u827e\u92b3\u751f\u6280\u7368\u5bb6\u6253\u9020\u51faMonoclonal-like Polyclonal Antibody\uff0c\u514d\u75ab\u539f(Immunogen)\u70ba\u61c9\u7528\u77ed\u93c8\u80dc\u80bd(peptide)\u70ba\u6297\u539f\u8a2d\u8a08\u51fa\u767c\u9ede\uff0c\u6311\u9078\u5404\u86cb\u767d\u6700\u4f73\u7684\u4f4d\u9ede(epitope) \u8fa8\u8b58\u4f4d\u4f86\u8fa8\u8a8d\u76ee\u6a19\u86cb\u767d\uff0ciReal\u00a0\u514d\u75ab\u8840\u6e05\u7d93\u904e\u4e00\u9023\u591a\u6b65\u9a5f\u7684\u7d14\u5316\u904e\u7a0b(Affinity column purified )\uff0c\u53bb\u9664\u6389\u5404\u7a2e\u975e\u5c08\u4e00\u6027\u7684\u86cb\u767d\uff0c\u4ee5\u964d\u4f4e\u6297\u9ad4\u7684\u80cc\u666f\u503c\uff1b\u540c\u6642\u85c9\u7531\u5154\u5b50\u7368\u7279\u7684\u514d\u75ab\u7cfb\u7d71\u6240\u7522\u751f\u7684\u6297\u9ad4\uff0c\u80fd\u5920\u7522\u2f63\u8a31\u591a\u2f7c\u2fcf\u514d\u75ab\u7cfb\u7d71\u4e0d\u80fd\u8b58\u5225\u7684\u6297\u539f\u7684\u6297\u9ad4\u3002\u64c1\u6709\u5404\u5b78\u8853\u55ae\u4f4d\u771f\u5be6\u6578\u64da\u7684\u9a57\u8b49\uff0c\u4e26\u5177\u5099\u81e8\u5e8a\u9a57\u8b49\u898f\u683c\u7684\u6297\u9ad4\u54c1\u8cea\uff0c\u5927\u91cf\u4e14\u73fe\u8ca8\u4f9b\u61c9\u3002<\/p>\n

COX2 antibody\u00a0<\/span>(Endogenous)\uff0c\u91dd\u5c0dCOX2\u5177\u6709\u5c08\u4e00\u8868\u73fe\uff0c\u53ef\u61c9\u7528\u65bcWestern Blot\u3001IF\u3001IHC(Paraffin)\u5be6\u9a57\uff0c\u7269\u7a2e\u4f86\u6e90\u70baRabbit-IgG\uff0c\u53cd\u61c9\u7269\u7a2e: Human\u3001Mouse\u3001Rat\u3002<\/p>\n

Weatern Blot\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/p>\n

Western Blot 1:500 – 1:1000
\nImmunofluorescence 1:200 \u2013 1:500
\nImmunohistochemistry (Paraffin) 1:100 \u2013 1:200<\/p>\n

COX2 \u6297\u9ad4Vimentin antibody\u7522\u54c1\u4ecb\u7d39:<\/span><\/strong><\/span><\/p>\n

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]<\/p>\n

\u524d\u5217\u817a\u7d20-\u5167\u904e\u6c27\u5316\u7269\u5408\u9176 (PTGS)\uff0c\u4e5f\u7a31\u70ba\u74b0\u52a0\u6c27\u9176\uff0c\u662f\u524d\u5217\u817a\u7d20\u751f\u7269\u5408\u6210\u4e2d\u7684\u95dc\u9375\u9176\uff0c\u540c\u6642\u5145\u7576\u96d9\u52a0\u6c27\u9176\u548c\u904e\u6c27\u5316\u7269\u9176\u3002PTGS \u6709\u5169\u7a2e\u540c\u5de5\u9176\uff1a\u7d44\u6210\u578b PTGS1 \u548c\u8a98\u5c0e\u578b PTGS2\uff0c\u5b83\u5011\u5728\u8868\u9054\u8abf\u7bc0\u548c\u7d44\u7e54\u5206\u4f48\u65b9\u9762\u6709\u6240\u4e0d\u540c\u3002\u8a72\u57fa\u56e0\u7de8\u78bc\u8a98\u5c0e\u578b\u540c\u5de5\u9176\u3002\u5b83\u53d7\u7279\u5b9a\u523a\u6fc0\u4e8b\u4ef6\u7684\u8abf\u7bc0\uff0c\u8868\u660e\u5b83\u8ca0\u8cac\u53c3\u8207\u708e\u75c7\u548c\u6709\u7d72\u5206\u88c2\u767c\u751f\u7684\u524d\u5217\u817a\u7d20\u985e\u751f\u7269\u5408\u6210\u3002[\u7531 RefSeq \u63d0\u4f9b\uff0c2009 \u5e74 2 \u6708]<\/p>\n

 <\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

\"IR109-446<\/p>\n

\u6297 COX2 \u6297\u9ad4 WB \u5716\u50cf(IREAL, #IR109-446)<\/span><\/strong><\/span>
\n\u6297 COX2 \u6297\u9ad4 – 1\/1000 \u7a00\u91cb
\n\u88c2\u89e3\u7269\uff0c\u6bcf\u9053 35 \u03bcg\u3002\u8a72\u5370\u8de1\u662f\u4f7f\u7528 7.5% SDS-PAGE \u751f\u6210\u7684\u3002\u7136\u5f8c\u5c07 NC \u819c\u5c01\u9589 1 \u5c0f\u6642\uff0c\u7136\u5f8c\u8207 IR109-446 \u5728 4\u00b0C \u4e0b\u5b75\u80b2\u904e\u591c\u3002<\/p>\n

\"IR109-446<\/p>\n

\u6297 COX2 \u6297\u9ad4 IHC \u5716\u50cf(IREAL, #IR109-446)<\/span><\/strong><\/span>
\n\u7528 1\/100 \u7684 IR109-446 \u6a19\u8a18 COX2 \u7684\u77f3\u881f\u5305\u57cb\u5c0f\u9f20\u7d44\u7e54\u7684\u514d\u75ab\u7d44\u7e54\u5316\u5b78\u5206\u6790<\/p>\n

\"IR109-446<\/p>\n

\u6297 COX2 \u6297\u9ad4 IHC \u5716\u50cf(IREAL, #IR109-446)<\/span><\/strong><\/span>
\n\u7528 1\/100 \u7684 IR109-446 \u6a19\u8a18 COX2 \u7684\u77f3\u881f\u5305\u57cb\u5c0f\u9f20\u7d44\u7e54\u7684\u514d\u75ab\u7d44\u7e54\u5316\u5b78\u5206\u6790<\/p>\n

\"IR109-446<\/p>\n

\u6297 COX2 \u6297\u9ad4 ICC\/IF \u5716\u50cf(IREAL, #IR109-446)<\/span><\/strong><\/span>
\n\u7d30\u80de\u5728\u5ba4\u6eab\u4e0b\u7528 4% PFA \u56fa\u5b9a 10 \u5206\u9418\uff0c\u5728\u5ba4\u6eab\u4e0b\u7528 0.1% NP-40 \u6ef2\u900f 10 \u5206\u9418\uff0c\u7136\u5f8c\u5728\u5ba4\u6eab\u4e0b\u7528 5% BSA \u5c01\u9589 30 \u5206\u9418\u3002\u7d30\u80de\u5728 1:300 \u548c 4\u00b0C \u4e0b\u7528 IR109-446 \u6297 COX2 \u6297\u9ad4\uff08\u7d05\u8272\uff09\u67d3\u8272\u3002DAPI\uff08\u85cd\u8272\uff09\u7528\u4f5c\u6838\u8907\u67d3\u5291\u3002<\/p>\n

\"IR109-446<\/p>\n

\u6297 COX2 \u6297\u9ad4 IHC \u5716\u50cf(IREAL, #IR109-446)<\/span><\/strong><\/span>
\n\u7528 1\/100 \u7684 IR109-446 \u6a19\u8a18 COX2 \u7684\u77f3\u881f\u5305\u57cb\u4eba\u764c\u7d44\u7e54\u7684\u514d\u75ab\u7d44\u7e54\u5316\u5b78\u5206\u6790<\/p>\n\n\n\n\n\n\n\n
IR300-981<\/td>\nCD9<\/a><\/span><\/td>\nExosome Biomarker<\/td>\nRabbit<\/td>\nH,M,R<\/td>\nWB, IF, IHC<\/td>\n<\/tr>\n
IR301-983<\/td>\nCD63<\/a><\/span><\/td>\nExosome Biomarker<\/td>\nRabbit<\/td>\nH,M,R<\/td>\nWB, IHC, FC<\/td>\n<\/tr>\n
IR302-986<\/td>\nCD81<\/a><\/span><\/td>\nExosome Biomarker<\/td>\nRabbit<\/td>\nH,M,R<\/td>\nWB, IF, IHC<\/td>\n<\/tr>\n
IR304-990<\/td>\nFlotillin-1<\/td>\nExosome Biomarker<\/td>\nRabbit<\/td>\nH,M,R<\/td>\nWB, IF, IHC<\/td>\n<\/tr>\n
IR303-988<\/td>\nALIX\u6297\u9ad4<\/a><\/span><\/td>\nExosome Biomarker<\/td>\nRabbit<\/td>\nH,M,R<\/td>\nWB, IF, IHC<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\"<\/p>\n

IHC\/\u75c5\u7406\u5b78\u6297\u9ad4(IHC\/Pathology antibodies)<\/span><\/strong><\/span><\/p>\n

Please note that related Positive Control Slides are also available. \u5206\u5b50\u75c5\u7406\u5b78(molecular pathology)\u4e2d\u7684\u514d\u75ab\u7d44\u7e54\u5316\u5b78(Immunohistochemistry)\u626e\u6f14\u91cd\u8981\u4f5c\u7528\uff1a\u8a3a\u65b7(Diagnosis)\u3001\u9810\u5f8c(Prognosis)\u548c\u9810\u6e2c(Predictive)\u3002 IHC \u6297\u9ad4(IHC antibodies)\u5df2\u88ab\u78ba\u5b9a\u70ba\u7528\u65bc\u798f\u723e\u99ac\u6797\u56fa\u5b9a\u77f3\u881f\u5207\u7247(formalin-fixed paraffin sections)\u514d\u75ab\u7d44\u7e54\u5316\u5b78(immunohistochemistry)\u7684\u6700\u4f73\u8a66\u5291\u3002\u6240\u6709 IHC\/\u75c5\u7406\u5b78\u6297\u9ad4(IHC\/Pathology antibodies)\u50c5\u4f9b\u7814\u7a76\u4f7f\u7528\u3002IHC\/\u75c5\u7406\u5b78\u6297\u9ad4\u5df2\u7d93\u91dd\u5c0d\u4eba\u9ad4\/\u8001\u9f20\/\u5927\u9f20\u7d44\u7e54\u7684\u514d\u75ab\u7d44\u7e54\u5316\u5b78\u9032\u884c\u4e86\u5ee3\u6cdb\u7684\u6e2c\u8a66\u548c\u9a57\u8b49(Pathology validated)\uff0c\u4e26\u7d93\u904e\u7cbe\u5fc3\u8a2d\u8a08\uff0c\u53ef\u63d0\u4f9b\u4e00\u81f4\u3001\u7279\u7570\u6027\u548c\u654f\u611f\u7684\u67d3\u8272\u3002<\/p>\n

PD-1\u75c5\u7406\u5b78\u6297\u9ad4\uff1a<\/span>\u7a0b\u5e8f\u6027\u6b7b\u4ea1 1 (Programmed Death 1, PD-1) \u662f T \u7d30\u80de\u8abf\u7bc0\u5291(T-cell regulators) CD28\/CTLA-4 \u5bb6\u65cf\u7684\u6210\u54e1\uff0c\u5728\u6d3b\u5316\u7684 T \u7d30\u80de\u3001B \u7d30\u80de\u548c\u5de8\u566c\u7d30\u80de\u8868\u9762\u8868\u9054\u70ba\u5171\u53d7\u9ad4(co-receptor)\u3002\u65b0\u7684\u7814\u7a76\u8868\u660e\uff0cPD-1\/PD-L1 \u4fe1\u865f\u901a\u8def\u53ef\u80fd\u8207\u6297\u816b\u7624\u514d\u75ab\u6709\u95dc\uff0c\u56e0\u70ba PD-L1 \u5df2\u88ab\u8b49\u660e\u53ef\u8a98\u5c0e\u6d3b\u5316 T \u7d30\u80de\u51cb\u4ea1(apoptosis)\u6216\u6291\u5236\u7d30\u80de\u6bd2\u6027 T \u7d30\u80de(cytotoxic T cells)\u7684\u6d3b\u6027\u3002\u8207 CD10 \u548c BCL6 \u4e0d\u540c\uff0cPD-1 \u50c5\u7531\u5c11\u6578 B \u7d30\u80de\u8868\u9054\uff0c\u56e0\u6b64\u6297 PD-1 \u53ef\u80fd\u662f\u8840\u7ba1\u514d\u75ab\u6bcd\u7d30\u80de\u6027\u6dcb\u5df4\u7624(angioimmunoblastic lymphoma)\u4e2d\u66f4\u7279\u7570\u548c\u6709\u7528\u7684\u8a3a\u65b7\u6a19\u8a8c\u7269\u3002>>\u00a0PD-L1\/CD274 C\u672b\u7aef\u6297\u9ad4<\/a><\/span><\/p>\n

CTLA4\u75c5\u7406\u5b78\u6297\u9ad4\uff1a<\/span>CD152 \u6297\u539f\uff0c\u4e5f\u7a31\u70ba CTLA4\uff0c\u662f\u4e00\u7a2e\u5c6c\u65bc\u514d\u75ab\u7403\u86cb\u767d\u8d85\u5bb6\u65cf(immunoglobulin superfamily)\u7684\u5b8c\u6574\u819c\u86cb\u767d\uff0c\u5176\u8868\u9054\u70ba 30 kDa \u55ae\u9ad4(30 kDa monomer)\u6216\u4e8c\u786b\u9375\u9023\u63a5\u7684\u540c\u578b\u4e8c\u805a\u9ad4(disulfide-linked homodimer)\u3002\u5b83\u8207T\u7d30\u80de\u6297\u539fCD28(T cell antigen CD28)\u5177\u6709\u540c\u6e90\u6027\uff0c\u8207CD28\u4e00\u6a23\uff0c\u662fCD80\u548cCD86\u7684\u914d\u9ad4(ligand)\u3002 CTLA-4 \u662f T \u8f14\u52a9\u7d30\u80de\u4e0a\u7684\u4e00\u7a2e\u53d7\u9ad4\uff0c\u53ef\u4f5c\u70ba\u514d\u75ab\u6aa2\u67e5\u9ede(immune checkpoint)\u548c\u514d\u75ab\u53cd\u61c9\u7684\u4e0b\u8abf\u56e0\u5b50\u3002 CTLA-4 \u7684\u7a81\u8b8a\u8207\u8a31\u591a\u81ea\u8eab\u514d\u75ab\u6027\u75be\u75c5(autoimmune diseases)\u6709\u95dc\u3002<\/p>\n

Lp-PLA2\u75c5\u7406\u5b78\u6297\u9ad4\uff1a<\/span>Lp-PLA2\uff0c\u4e5f\u7a31\u70ba\u8102\u86cb\u767d\u76f8\u95dc\u78f7\u8102\u9176 A2 (Lipoprotein-associated Phospholipase A2)\u6216\u8840\u5c0f\u677f\u6d3b\u5316\u56e0\u5b50\u4e59\u9170\u6c34\u89e3\u9176 (platelet-activating factor acetylhydrolase , PAF-AH)\uff0c\u662f\u4e00\u7a2e\u7531\u708e\u75c7\u7d30\u80de(inflammatory cells )\u7522\u751f\u7684\u9176\uff0c\u5176\u529f\u80fd\u662f\u4f7f\u8840\u5c0f\u677f\u6d3b\u5316\u56e0\u5b50(platelet-activating factor)\u5931\u6d3b\u548c\u964d\u89e3(degrade)\uff0c\u8840\u5c0f\u677f\u6d3b\u5316\u56e0\u5b50\u662f\u4e00\u7a2e\u6709\u6548\u7684\u4fc3\u708e\u6027\u78f7\u8102(pro-inflammatory phospholipid)\uff0c\u540c\u6642\u9084\u8ca0\u8cac\u6c34\u89e3\u6c27\u5316\u4fee\u98fe(hydrolyzing oxidatively modified)\u7684\u591a\u4e0d\u98fd\u548c\u8102\u80aa\u9178(polyunsaturated fatty acids)\u3002 Lp-PLA2 \u7684\u9176\u4fc3\u4f5c\u7528\u7522\u7269\u8207\u52d5\u8108\u7ca5\u6a23\u786c\u5316(atherosclerosis)\u7684\u767c\u5c55\u6709\u95dc\uff0c\u4e26\u8207\u51a0\u5fc3\u75c5(coronary heart disease)\u548c\u4e2d\u98a8(stroke)\u7684\u98a8\u96aa\u589e\u52a0\u5448\u6b63\u76f8\u95dc\u3002<\/p>\n

Muc2\u75c5\u7406\u5b78\u6297\u9ad4\uff1a<\/span>\u7c98\u86cb\u767d 2(Mucin 2)\uff0c\u4e5f\u7a31\u70ba MUC2\uff0c\u662f\u4e00\u7a2e\u7de8\u78bc\u7c98\u86cb\u767d\u5bb6\u65cf(mucin protein family)\u6210\u54e1\u7684\u4eba\u985e\u57fa\u56e0\u3002\u7531\u8a72\u57fa\u56e0\u7de8\u78bc\u7684\u86cb\u767d\u8cea\u5f62\u6210\u4fdd\u8b77\u8178\u8154(gut lumen)\u7684\u4e0d\u6eb6\u6027\u7c98\u6db2\u5c4f\u969c\u3002\u5728\u6b63\u5e38\u7d50\u8178(normal colon)\u3001\u4e73\u817a(breast)\u3001\u524d\u5217\u817a(prostate)\u548c\u553e\u6db2\u817a(salivary gland)\u7b49\u4eba\u9ad4\u7d44\u7e54\u4ee5\u53ca\u80c3\u8178\u9053(gastrointestinal)\u3001\u7d50\u8178(colonic)\u3001\u4e73\u817a(breast)\u548c\u524d\u5217\u817a\u816b\u7624(prostate neoplasia)\u4e2d\u6aa2\u6e2c\u5230 MUC2 \u8868\u9054\u3002<\/p>\n

\u7d30\u80de\u89d2\u86cb\u767d 18 (Cytokeratin 18) \u75c5\u7406\u5b78\u6297\u9ad4\uff1a<\/span>\u7d30\u80de\u89d2\u86cb\u767d 18 (Cytokeratin 18, CK 18) \u662f\u4e00\u7a2e 45kDa \u7684\u86cb\u767d\u8cea\uff0c\u5c6c\u65bc\u9178\u6027\u985e\u578b\u7684\u7d30\u80de\u89d2\u86cb\u767d(cytokeratins)\uff0c\u901a\u5e38\u5728\u7c21\u55ae\u7684\u975e\u5206\u5c64\u4e0a\u76ae\u7d30\u80de(non-stratified epithelia)\u4e2d\u8868\u9054\u3002\u7136\u800c\uff0cCK 18 \u4e5f\u5728\u79fb\u884c\u4e0a\u76ae(transitional epithelium)\u7684\u57fa\u5e95\u7d30\u80de\u548c\u8868\u5c64\u7d30\u80de\u4ee5\u53ca\u8907\u96dc\u4e0a\u76ae(complex epithelia)\u7684\u7ba1\u8154\/\u5206\u6ccc\u7d30\u80de(luminal\/secretory cells)\u4e2d\u8868\u9054\u3002 CK 18 \u6297\u9ad4\u5728\u8d77\u6e90\u65bc\u7c21\u55ae\u548c\u817a\u9ad4\u4e0a\u76ae(glandular epithelium)\u7684\u817a\u764c(Adenocarcinomas)\u4ee5\u53ca\u9c57\u72c0\u7d30\u80de\u764c(Squamous Carcinoma)\u7684\u4f4e\u5206\u5316\u816b\u7624\u7d30\u80de\u4e2d\u5448\u967d\u6027\u67d3\u8272\u3002<\/p>\n

MLH1\u75c5\u7406\u5b78\u6297\u9ad4\u00a0<\/span>\u662f\u4e00\u7a2e\u932f\u914d\u4fee\u5fa9\u86cb\u767d(mismatch repair protein)\uff0c\u8207 MSH2\u3001MSH6 \u548c PMS2 \u4e00\u8d77\u53c3\u8207\u7dad\u6301\u907a\u50b3\u4fe1\u606f\u7684\u5b8c\u6574\u6027\u3002\u9019\u7a2e\u86cb\u767d\u8cea\u901a\u5e38\u8207\u907a\u50b3\u6027(hereditary)\u975e\u606f\u8089\u75c5\u6027\u7d50\u76f4\u8178\u764c(non-polyposis colorectal cancer)\u6709\u95dc\uff0c\u56e0\u70ba MLH1 \u57fa\u56e0\u5728\u60a3\u6709\u9019\u7a2e\u764c\u75c7\u7684\u60a3\u8005\u4e2d\u7d93\u5e38\u767c\u751f\u7a81\u8b8a\u3002\u7814\u7a76\u8868\u660e\uff0cMLH1 \u5728\u5927\u90e8\u5206\u5fae\u885b\u661f\u4e0d\u7a69\u5b9a\u6027(microsatellite instability)\u4ee5\u53ca\u5b50\u5bae\u5167\u819c\u764c(endometrial)\u548c\u5375\u5de2\u764c(ovarian cancers)\u60a3\u8005\u4e2d\u5b58\u5728\u7f3a\u9677\u3002\u7576\u5728 IHC \u9762\u677f\u4e2d\u8207 MSH6\u3001MSH2 \u548c PMS2 \u914d\u5c0d\u6642\uff0c\u6297 MLH1 \u7684\u4f7f\u7528\u5f97\u5230\u512a\u5316\u3002<\/p>\n

HER2\u75c5\u7406\u5b78\u6297\u9ad4:<\/span><\/p>\n

HER2\u5728 10-20% \u7684\u4e73\u817a\u764c\u60a3\u8005(breast cancer patients)\u4e2d\u88ab\u8b58\u5225\u51fa\u4f86\u3002 Her2\/Neu (c-erbB-2) \u539f\u764c\u57fa\u56e0(proto-oncogene)\u662f\u4e00\u7a2e\u8de8\u819c\u53d7\u9ad4\u916a\u6c28\u9178\u6fc0\u9176(transmembrane receptor tyrosine kinase)\uff0c\u5728\u8a31\u591a\u764c\u75c7\u4e2d\u90fd\u6709\u81e8\u5e8a\u8868\u73fe\u3002 c-erbB-2 \u86cb\u767d\u7684\u904e\u5ea6\u8868\u9054(Overexpression)\u8207\u5c0e\u7ba1\u4e73\u817a\u764c(ductal breast cancer)\u4ee5\u53ca\u80ba\u817a\u764c(pulmonary)\u548c\u80c3\u817a\u764c(gastric adenocarcinomas)\u6709\u95dc\u3002Her2 \u548c p53 \u4e4b\u9593\u7684\u76f8\u95dc\u6027\u4e5f\u5df2\u88ab\u8b49\u660e\uff0c\u56e0\u70ba\u9019\u5169\u7a2e\u86cb\u767d\u7684\u904e\u5ea6\u8868\u9054\u8207\u8180\u80f1\u764c\u65e9\u671f\u4fb5\u8972(early invasion)\u548c\u8f49\u79fb(metastasis)\u6709\u95dc\u3002<\/p>\n

Ki-67\u00a0<\/span>\u662f\u4e00\u7a2e\u6838\u975e\u7d44\u86cb\u767d(nuclear non-histone protein)\uff0c\u5728\u975c\u6b62\u7d30\u80de(quiescent cells)\u4e2d\u4ee5\u4f4e\u6c34\u5e73\u5b58\u5728\uff0c\u4f46\u5728\u589e\u6b96\u7d30\u80de(proliferating cells)\u4e2d\u589e\u52a0\uff0c\u5c24\u5176\u662f\u5728 G2\u3001M \u548c S \u671f\u5f8c\u534a\u671f\u3002\u56e0\u6b64\uff0cKi-67 \u53cd\u61c9\u6027\uff08\u5b9a\u7fa9\u70ba\u901a\u904e IHC \u67d3\u8272\u6e2c\u91cf\u7684\u67d3\u8272\u967d\u6027\u7684\u816b\u7624\u7d30\u80de\u767e\u5206\u6bd4\uff09\u662f\u7d30\u80de\u589e\u6b96(cell proliferation)\u7684\u7279\u7570\u6027\u6838\u6a19\u8a18(nuclear marker )\u3002\u904e\u5ea6\u8868\u9054\u7d93\u5e38\u51fa\u73fe\u5728\u5404\u7a2e\u60e1\u6027\u7d44\u7e54(malignant tissues )\u4e2d\uff0c\u4e26\u4e14\u8207\u60a3\u6709\u795e\u7d93\u5167\u5206\u6ccc\u816b\u7624(neuroendocrine tumor)\u7684\u500b\u9ad4\u7684\u8f03\u5dee\u5b58\u6d3b\u7387\u76f8\u95dc\u3002>>IHC\u75c5\u7406\u5b78\u6297\u9ad4-Ki-67\u00a0\u6297\u9ad4<\/a>\u00a0<\/span><\/strong><\/span><\/p>\n

\"Better<\/p>\n

Exosome \u7814\u7a76\u5de5\u5177\/\u764c\u75c7\u76f8\u95dcExosomal Protein Marker<\/strong><\/span>–IReal Biotechnology<\/span><\/span><\/p>\n

IREAL\u63d0\u4f9b\u591a\u7a2e\u9ad8\u8cea\u91cf\u5916\u6ccc\u9ad4(exosomes)\u55ae\u591a\u682a\u6297\u9ad4 (IREAL Antibody)\uff0c\u5305\u62ecCD9(#IR300-981)<\/a><\/span>\u3001CD63(#IR301-983)<\/a><\/span>\u3001CD81(#IR302-986)<\/a><\/span>\u3001Flotillin-1(#IR304-990)<\/a>\u3001Alix(#IR303-988)<\/a><\/span>\u7b2c\u4e09\u65b9\u9a57\u8b49IHC\/IF\/WB\u6297\u9ad4\u3002\u5916\u6ccc\u9ad4(exosomes)\u53e6\u6709\u63d0\u4f9b\u5957\u7d44\u578b\u5f0f\u7684\u6297\u9ad4Panel\u5957\u7d44<\/a>\u3002<\/span><\/p>\n

\u8b58\u5225\u5916\u6ccc\u9ad4(exosomes)\u7684\u6700\u5e38\u898b\u8de8\u819c\u6a19\u8a18\u4f86\u81ea\u56db\u8de8\u819c\u86cb\u767d\u5bb6\u65cf\uff08CD63 \u3001CD81 \u548c CD9\uff09\u3002\u8a72\u5bb6\u65cf\u4e2d\u7684\u86cb\u767d\u8cea\u5177\u6709\u56db\u500b\u8de8\u819c\u5340\u57df\uff0c\u4e26\u4e14\u5b9a\u4f4d\u65bc\u8cea\u819c\u4e2d\u5bcc\u542b\u56db\u8de8\u819c\u86cb\u767d\u7684\u5fae\u57df (\u00a0tetraspanin enriched microdomains, TEM)\u3002\uff08CD63 \u3001CD81 \u3001 CD9\u53caCD82\uff09\u8207\u7c98\u9644\u5206\u5b50(\u00a0adhesion molecules)\u3001\u8de8\u819c\u53d7\u9ad4(\u00a0transmembrane receptors)\u548c\u7d30\u80de\u5167\u4fe1\u865f\u86cb\u767d(intracellular signaling proteins)\u76f8\u95dc\uff0c\u4ee5\u4fc3\u9032\u548c\u8abf\u7bc0\u4fe1\u865f(\u00a0signaling events)\u4e8b\u4ef6\u3002\u9ad8\u8cea\u91cf\u5916\u6ccc\u9ad4(exosomes)\u55ae\u591a\u682a\u6297\u9ad4 (IREAL Antibody)\uff0c\u63d0\u4f9b\u60a8\u66f4\u591aExosome \u7814\u7a76\u5de5\u5177\uff0c\u53ef\u61c9\u7528\u65bcIHC\/IF\/WB\u6297\u9ad4\u3002<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716<\/p>\n

BRAND\/AGENCY\/<\/strong>\u53f0\u7063\u4ee3\u7406\u5546<\/strong><\/span>\/<\/span>\u00a0<\/a><\/span><\/strong>IREAL<\/strong>\u827e\u92b3\u53f0\u7063\u4ee3\u7406\u5546<\/strong><\/a><\/span><\/span><\/p>\n

IREAL\u827e\u92ed\u5b98\u7db2\u00a0https:\/\/www.irealbio.com\/<\/a><\/span><\/p>\n

IREAL\u827e\u92ed\u751f\u7269\u79d1\u6280\u63d0\u4f9b\u6642\u4e0b\u71b1\u9580\u7814\u7a76\u9818\u57dfEMT marker\u3001Apoptosis\u3001ER stress\u3001Cell Cycle\u3001Autophagy\u53caNeuroscience\u7b49\u5e38\u7528\u6297\u9ad4\uff0c\u4e26\u63d0\u4f9b\u5ba2\u88fd\u5316\u6297\u9ad4\u670d\u52d9\u5e73\u53f0\u3002iReal\u00a0\u827e\u92ed\u751f\u7269\u79d1\u6280\u63d0\u4f9b\u6642\u4e0b\u71b1\u9580\u7814\u7a76\u9818\u57dfEMT marker\u3001Apoptosis\u3001ER stress\u3001Cell Cycle\u3001Autophagy\u53caNeuroscience\u7b49\u5e38\u7528\u6297\u9ad4\uff0c\u4e26\u63d0\u4f9b\u5ba2\u88fd\u5316\u6297\u9ad4\u670d\u52d9\u5e73\u53f0\u3002iReal \u827e\u92b3\u751f\u6280\u7368\u5bb6\u6253\u9020\u51faMonoclonal-like Polyclonal Antibody\uff0c\u514d\u75ab\u539f(Immunogen)\u70ba\u61c9\u7528\u77ed\u93c8\u80dc\u80bd(peptide)\u70ba\u6297\u539f\u8a2d\u8a08\u51fa\u767c\u9ede\uff0c\u6311\u9078\u5404\u86cb\u767d\u6700\u4f73\u7684\u4f4d\u9ede(epitope) \u8fa8\u8b58\u4f4d\u4f86\u8fa8\u8a8d\u76ee\u6a19\u86cb\u767d\uff0ciReal\u00a0\u514d\u75ab\u8840\u6e05\u7d93\u904e\u4e00\u9023\u591a\u6b65\u9a5f\u7684\u7d14\u5316\u904e\u7a0b(Affinity column purified )\uff0c\u53bb\u9664\u6389\u5404\u7a2e\u975e\u5c08\u4e00\u6027\u7684\u86cb\u767d\uff0c\u4ee5\u964d\u4f4e\u6297\u9ad4\u7684\u80cc\u666f\u503c\uff1b\u540c\u6642\u85c9\u7531\u5154\u5b50\u7368\u7279\u7684\u514d\u75ab\u7cfb\u7d71\u6240\u7522\u751f\u7684\u6297\u9ad4\uff0c\u80fd\u5920\u7522\u2f63\u8a31\u591a\u2f7c\u2fcf\u514d\u75ab\u7cfb\u7d71\u4e0d\u80fd\u8b58\u5225\u7684\u6297\u539f\u7684\u6297\u9ad4\u3002\u64c1\u6709\u5404\u5b78\u8853\u55ae\u4f4d\u771f\u5be6\u6578\u64da\u7684\u9a57\u8b49\uff0c\u4e26\u5177\u5099\u81e8\u5e8a\u9a57\u8b49\u898f\u683c\u7684\u6297\u9ad4\u54c1\u8cea\uff0c\u5927\u91cf\u4e14\u73fe\u8ca8\u4f9b\u61c9IHC\/IF\/WB\u6297\u9ad4\u3002<\/p>\n

\"IREAL\u827e\u92b3\u514d\u75ab\u67d3\u8272\u6297\u9ad4-\u592a\u9f0e\u751f\u6280_\u5de5\u4f5c\u5340\u57df<\/p>\n

\"\u578b\u9304\u4e0b\u8f09\"<\/p>\n

\"\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

IHC\/WB\/IF\/IP\u7814\u7a76\u5de5\u5177-Inflammatory\/ Inflammasome Antibodies& […]<\/p>\n","protected":false},"author":3,"featured_media":38534,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5264],"tags":[5446],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40025"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=40025"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40025\/revisions"}],"predecessor-version":[{"id":40094,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40025\/revisions\/40094"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/38534"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=40025"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=40025"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=40025"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}