{"id":40013,"date":"2023-03-13T15:32:05","date_gmt":"2023-03-13T07:32:05","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=40013"},"modified":"2023-03-28T11:45:48","modified_gmt":"2023-03-28T03:45:48","slug":"sqstm1-p62%e6%8a%97%e9%ab%94-sqstm1-p62-antibody%e8%b2%a8%e8%99%9fir91-384-ireal-biotechnology-%e8%89%be%e9%8b%ad%e7%94%9f%e6%8a%80","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=40013","title":{"rendered":"SQSTM1 \/ p62\u6297\u9ad4 | SQSTM1 \/ p62 antibody\u8ca8\u865fIR91-384 (IReal Biotechnology \u827e\u92ed\u751f\u6280)"},"content":{"rendered":"

IHC\/WB\/IF\/IP\u7814\u7a76\u5de5\u5177-Autophagy\u00a0\/ ER stress\u00a0Antibodies<\/strong><\/span>–IReal Biotechnology<\/span><\/span><\/p>\n

Sequestosome 1 (SQSTM1, p62) \u662f\u4e00\u7a2e\u6cdb\u7d20\u7d50\u5408\u86cb\u767d(ubiquitin binding protein )\uff0c\u53c3\u8207\u7d30\u80de\u4fe1\u865f\u8f49\u5c0e(cell signaling)\u3001\u6c27\u5316\u61c9\u6fc0(oxidative stress)\u548c\u81ea\u566c(autophagy) (1-4)\u3002\u5b83\u9996\u5148\u88ab\u9451\u5b9a\u70ba\u4e00\u7a2e\u8207 p56Lck \u7684 SH2 \u7d50\u69cb\u57df\u7d50\u5408\u7684\u86cb\u767d\u8cea (5)\uff0c\u4e26\u7368\u7acb\u5730\u88ab\u767c\u73fe\u8207 PKC\u03b6 \u76f8\u4e92\u4f5c\u7528 (6,7)\u3002\u96a8\u5f8c\u767c\u73fe SQSTM1 \u8207\u6cdb\u7d20(ubiquitin)\u76f8\u4e92\u4f5c\u7528\uff0c\u70ba\u591a\u7a2e\u4fe1\u865f\u86cb\u767d(signaling proteins)\u63d0\u4f9b\u652f\u67b6(scaffold)\u4e26\u901a\u904e\u86cb\u767d\u9176\u9ad4\u6216\u6eb6\u9176\u9ad4\u89f8\u767c\u86cb\u767d\u8cea\u964d\u89e3 (8)\u3002 SQSTM1 \u548c TRAF6 \u4e4b\u9593\u7684\u76f8\u4e92\u4f5c\u7528\u5c0e\u81f4 TRAF6 \u7684 K63 \u9023\u63a5\u591a\u805a\u6cdb\u7d20\u5316\u548c\u96a8\u5f8c\u7684 NF-\u03baB \u901a\u8def\u6fc0\u6d3b (9)\u3002\u7531 SQSTM1 \u5f62\u6210\u7684\u86cb\u767d\u8cea\u805a\u96c6\u9ad4\u53ef\u88ab\u81ea\u566c\u9ad4\u964d\u89e3 (4,10,11)\u3002 SQSTM1 \u7d50\u5408\u81ea\u566c\u9ad4\u819c\u86cb\u767d(autophagosomal membrane protein)\u00a0 LC3\/Atg8\uff0c\u5c07\u542b\u6709 SQSTM1 \u7684\u86cb\u767d\u805a\u96c6\u9ad4\u5e36\u5230\u81ea\u566c\u9ad4(autophagosome)(12)\u3002\u81ea\u566c\u9ad4((autophagosome))\u7684\u6eb6\u9176\u9ad4\u964d\u89e3(Lysosomal degradation)\u5c0e\u81f4\u81ea\u566c\u904e\u7a0b\u4e2d SQSTM1 \u6c34\u5e73\u964d\u4f4e\uff1b\u76f8\u53cd\uff0c\u81ea\u566c\u6291\u88fd\u5291(\u00a0autophagy inhibitors)\u53ef\u7a69\u5b9a SQSTM1 \u6c34\u5e73\u3002\u7814\u7a76\u8868\u660e SQSTM1 \u8207\u6c27\u5316\u61c9\u6fc0(oxidative stress)\u4e4b\u9593\u5b58\u5728\u806f\u7e6b\u3002 SQSTM1 \u8207 KEAP1 \u76f8\u4e92\u4f5c\u7528\uff0cKEAP1 \u662f NRF2 \u7684\u7d30\u80de\u8cea\u6291\u5236\u5291\uff0cNRF2 \u662f\u53c3\u8207\u7d30\u80de\u5c0d\u6c27\u5316\u61c9\u6fc0(oxidative stress)\u53cd\u61c9\u7684\u95dc\u9375\u8f49\u9304\u56e0\u5b50(transcription factor) (3)\u3002\u56e0\u6b64\uff0cSQSTM1 \u7684\u7a4d\u7d2f\u53ef\u5c0e\u81f4 NRF2 \u6d3b\u6027\u589e\u52a0\u3002<\/p>\n

\"\"<\/p>\n

\"HOT\"<\/a>SQSTM1 \/ p62\u6297\u9ad4 | SQSTM1 \/ p62 antibody\u8ca8\u865fIR91-384\u00a0 (IReal Biotechnology \u827e\u92ed\u751f\u6280)<\/a><\/span><\/span><\/strong><\/span><\/p>\n

SQSTM1 \/ p62 antibody (IReal Biotechnology \u827e\u92ed\u751f\u6280,IR91-384)<\/p>\n

iReal \u827e\u92b3\u751f\u6280\u7368\u5bb6\u6253\u9020\u51faMonoclonal-like Polyclonal Antibody\uff0c\u514d\u75ab\u539f(Immunogen)\u70ba\u61c9\u7528\u77ed\u93c8\u80dc\u80bd(peptide)\u70ba\u6297\u539f\u8a2d\u8a08\u51fa\u767c\u9ede\uff0c\u6311\u9078\u5404\u86cb\u767d\u6700\u4f73\u7684\u4f4d\u9ede(epitope) \u8fa8\u8b58\u4f4d\u4f86\u8fa8\u8a8d\u76ee\u6a19\u86cb\u767d\uff0ciReal\u00a0\u514d\u75ab\u8840\u6e05\u7d93\u904e\u4e00\u9023\u591a\u6b65\u9a5f\u7684\u7d14\u5316\u904e\u7a0b(Affinity column purified )\uff0c\u53bb\u9664\u6389\u5404\u7a2e\u975e\u5c08\u4e00\u6027\u7684\u86cb\u767d\uff0c\u4ee5\u964d\u4f4e\u6297\u9ad4\u7684\u80cc\u666f\u503c\uff1b\u540c\u6642\u85c9\u7531\u5154\u5b50\u7368\u7279\u7684\u514d\u75ab\u7cfb\u7d71\u6240\u7522\u751f\u7684\u6297\u9ad4\uff0c\u80fd\u5920\u7522\u2f63\u8a31\u591a\u2f7c\u2fcf\u514d\u75ab\u7cfb\u7d71\u4e0d\u80fd\u8b58\u5225\u7684\u6297\u539f\u7684\u6297\u9ad4\u3002\u64c1\u6709\u5404\u5b78\u8853\u55ae\u4f4d\u771f\u5be6\u6578\u64da\u7684\u9a57\u8b49\uff0c\u4e26\u5177\u5099\u81e8\u5e8a\u9a57\u8b49\u898f\u683c\u7684\u6297\u9ad4\u54c1\u8cea\uff0c\u5927\u91cf\u4e14\u73fe\u8ca8\u4f9b\u61c9\u3002<\/p>\n

SQSTM1 \/ p62 antibody\u00a0<\/span>(Endogenous)\uff0c\u91dd\u5c0dSQSTM1 \/ p62\u5177\u6709\u5c08\u4e00\u8868\u73fe\uff0c\u53ef\u61c9\u7528\u65bcWestern Blot\u3001IF\u3001IHC(Paraffin)\u3001Pathology\u5be6\u9a57\uff0c\u7269\u7a2e\u4f86\u6e90\u70baRabbit-IgG\uff0c\u53cd\u61c9\u7269\u7a2e: Human\u3001Mouse\u3001Rat\u3002<\/p>\n

Weatern Blot\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/p>\n

Western Blot 1:1000-1:2000
\nImmunofluorescence 1:100-1:400
\nImmunohistochemistry (Paraffin) 1: 100-1:200<\/p>\n

SQSTM1 \/ p62\u6297\u9ad4Vimentin antibody\u7522\u54c1\u4ecb\u7d39:<\/span><\/strong><\/span><\/p>\n

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding\/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]<\/p>\n

\u8a72\u57fa\u56e0\u7de8\u78bc\u4e00\u7a2e\u591a\u529f\u80fd\u86cb\u767d\uff0c\u53ef\u7d50\u5408\u6cdb\u7d20\u4e26\u8abf\u7bc0\u6838\u56e0\u5b50 kappa-B (NF-kB) \u4fe1\u865f\u901a\u8def\u7684\u6fc0\u6d3b\u3002\u8a72\u86cb\u767d\u8cea\u4f5c\u70ba\u652f\u67b6\/\u929c\u63a5\u86cb\u767d\u8207 TNF \u53d7\u9ad4\u76f8\u95dc\u56e0\u5b50 6 \u5354\u540c\u4f5c\u7528\uff0c\u4ee5\u97ff\u61c9\u4e0a\u6e38\u4fe1\u865f\u4ecb\u5c0e NF-kB \u7684\u6fc0\u6d3b\u3002\u5df2\u7d93\u70ba\u8a72\u57fa\u56e0\u9451\u5b9a\u4e86\u7de8\u78bc\u76f8\u540c\u6216\u4e0d\u540c\u540c\u7a2e\u578b\u7684\u53ef\u8b8a\u526a\u63a5\u8f49\u9304\u7269\u8b8a\u9ad4\u3002\u8a72\u57fa\u56e0\u7684\u7a81\u8b8a\u6703\u5c0e\u81f4\u6563\u767c\u6027\u548c\u5bb6\u65cf\u6027\u4f69\u5409\u7279\u9aa8\u75c5\u3002[\u7531 RefSeq \u63d0\u4f9b\uff0c2009 \u5e74 3 \u6708]<\/p>\n

 <\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

\"IR91-384<\/p>\n

\u6297 SQSTM1 \/ p62 \u6297\u9ad4(IREAL, #IR91-384)<\/span><\/strong><\/span>
\n\u6297 SQSTM1 \/ p62 \u6297\u9ad4 – 1\/1000 \u7a00\u91cb\u3002\u6bcf\u6cf3\u9053 35 \u03bcg \u7684\u88c2\u89e3\u7269\u3002
\n\u8a72\u5370\u8de1\u662f\u4f7f\u7528 12% SDS-PAGE \u751f\u6210\u7684\u3002\u7136\u5f8c\u5c07\u785d\u9178\u7e96\u7dad\u7d20\u819c\u5c01\u9589 1 \u5c0f\u6642\uff0c\u7136\u5f8c\u8207 IR91-384 \u5728 4\u00b0C \u4e0b\u5b75\u80b2\u904e\u591c\u3002<\/p>\n

\"IR91-384<\/p>\n

\u6297 SQSTM1 \/ p62 \u6297\u9ad4 IHC \u5716\u50cf(IREAL, #IR91-384)<\/span><\/strong><\/span>
\n\u4f7f\u7528 IR91-384 \u5728 1\/200 RT 1 \u5c0f\u6642\u5c0d\u77f3\u881f\u5305\u57cb\u7684\u5c0f\u9f20\u814e\u81df\u6a19\u8a18 SQS\/TM1 \u200b\u200b\/ P62 \u9032\u884c\u514d\u75ab\u7d44\u7e54\u5316\u5b78\u5206\u6790\u3002\u8868\u4f4d\u6aa2\u7d22\u65b9\u6cd5\uff1a\u6ab8\u6aac\u9178\u9e7d\u7de9\u885d\u6db2\uff0cpH 6.0<\/p>\n

\"IR91-384<\/p>\n

\u6297 SQSTM1 \/ p62 \u6297\u9ad4 IHC \u5716\u50cf(IREAL, #IR91-384)<\/span><\/strong><\/span>
\n\u4f7f\u7528 IR91-384 \u5728 1\/200 RT 1 \u5c0f\u6642\u5c0d\u77f3\u881f\u5305\u57cb\u7684\u5c0f\u9f20\u813e\u81df\u6a19\u8a18 SQS\/TM1 \u200b\u200b\/ P62 \u9032\u884c\u514d\u75ab\u7d44\u7e54\u5316\u5b78\u5206\u6790\u3002\u8868\u4f4d\u6aa2\u7d22\u65b9\u6cd5\uff1a\u6ab8\u6aac\u9178\u9e7d\u7de9\u885d\u6db2\uff0cpH 6.0<\/p>\n

\"IR91-384<\/p>\n

\u6297 SQSTM1\/p62 \u6297\u9ad4 ICC \u5716\u50cf(IREAL, #IR91-384)<\/span><\/strong><\/span>
\n\u7d30\u80de\u5728\u5ba4\u6eab\u4e0b\u7528 4% PFA \u56fa\u5b9a 10 \u5206\u9418\uff0c\u5728\u5ba4\u6eab\u4e0b\u7528 0.1% NP-40 \u6ef2\u900f 10 \u5206\u9418\uff0c\u7136\u5f8c\u5728\u5ba4\u6eab\u4e0b\u7528 5% BSA \u5c01\u9589 30 \u5206\u9418\u3002\u7d30\u80de\u5728 1:200 \u548c 4\u00b0C \u4e0b\u7528 IR91-384 \u6297 SQSTM1 \/ p62 \u6297\u9ad4\uff08\u7d05\u8272\uff09\u67d3\u8272\u3002DAPI\uff08\u85cd\u8272\uff09\u7528\u4f5c\u6838\u8907\u67d3\u5291\u3002<\/p>\n

\"IR91-384\u6297SQSTM1\/p62\u6297\u9ad4_\u7d50\u76f4\u8178\u764cIHC\u5716\u50cf\"<\/p>\n

\u6297SQSTM1\/p62\u6297\u9ad4_\u7d50\u76f4\u8178\u764cIHC\u5716\u50cf(IREAL, #IR91-384)<\/span><\/strong><\/span>
\n\u77f3\u881f\u5305\u57cb\u7684\u4eba\u7d50\u76f4\u8178\u764c\u7d44\u7e54\u7528 1\/200 \u7684 IR91-384 \u6a19\u8a18 SQSTM1\/p62 \u7684\u514d\u75ab\u7d44\u7e54\u5316\u5b78\u5206\u6790\u3002<\/p>\n

\"IR91-384<\/p>\n

\u6297 SQSTM1 \/ p62 \u6297\u9ad4 IHC \u5716\u50cf(IREAL, #IR91-384)<\/span><\/strong><\/span>
\n\u514d\u75ab\u7192\u5149\uff1a\u7d30\u80de\u5728\u5ba4\u6eab\u4e0b\u7528 4% \u591a\u805a\u7532\u919b\u56fa\u5b9a 10 \u5206\u9418\uff0c\u5728\u5ba4\u6eab\u4e0b\u7528 0.1% NP-40 \u900f\u5316 10 \u5206\u9418\uff0c\u7136\u5f8c\u5728\u5ba4\u6eab\u4e0b\u7528 5% BSA \u5c01\u9589 30 \u5206\u9418\u3002\u7d30\u80de\u5728 1:100 \u548c 4\u00b0C \u4e0b\u7528 IR91-384 \u6297 SQSTM1 \/ p62 \u6297\u9ad4\uff08\u7d05\u8272\uff09\u67d3\u8272\u3002DAPI\uff08\u85cd\u8272\uff09\u7528\u4f5c\u6838\u8907\u67d3\u5291\u3002<\/p>\n

\"IR91-384<\/p>\n

\u6297 SQSTM1 \/ p62 \u6297\u9ad4 IHC \u5716\u50cf(IREAL, #IR91-384)<\/span><\/strong><\/span>
\n\u4f7f\u7528 IR91-384 \u5728 1\/100 RT 1 \u5c0f\u6642\u5c0d\u77f3\u881f\u5305\u57cb\u7684\u5c0f\u9f20\u7d44\u7e54\u6a19\u8a18 SQS-TM1 \/ p62 \u9032\u884c\u514d\u75ab\u7d44\u7e54\u5316\u5b78\u5206\u6790\u3002
\n\u8868\u4f4d\u4fee\u5fa9\u65b9\u6cd5\uff1a\u6ab8\u6aac\u9178\u9e7d\u7de9\u885d\u6db2\uff0cpH 6.0<\/p>\n

\"IR91-384<\/p>\n

\u6297 SQSTM1 \/ p62 \u6297\u9ad4 IHC \u5716\u50cf(IREAL, #IR91-384)<\/span><\/strong><\/span>
\n\u4f7f\u7528 IR91-384 \u5728 1\/100 RT 1 \u5c0f\u6642\u5c0d\u77f3\u881f\u5305\u57cb\u7684\u5c0f\u9f20\u7d44\u7e54\u6a19\u8a18 SQS-TM1 \/ p62 \u9032\u884c\u514d\u75ab\u7d44\u7e54\u5316\u5b78\u5206\u6790\u3002
\n\u8868\u4f4d\u4fee\u5fa9\u65b9\u6cd5\uff1a\u6ab8\u6aac\u9178\u9e7d\u7de9\u885d\u6db2\uff0cpH 6.0<\/p>\n

\"IR91-384<\/p>\n

\u6297 SQSTM1 \/ p62 \u6297\u9ad4_HT-29 ICC\/ IF \u5716\u50cf(IREAL, #IR91-384)<\/span><\/strong><\/span>
\n\u514d\u75ab\u7192\u5149\uff1a\u7d30\u80de\u5728\u5ba4\u6eab\u4e0b\u7528 4% \u591a\u805a\u7532\u919b\u56fa\u5b9a 10 \u5206\u9418\uff0c\u5728\u5ba4\u6eab\u4e0b\u7528 0.1% NP-40 \u900f\u5316 10 \u5206\u9418\uff0c\u7136\u5f8c\u5728\u5ba4\u6eab\u4e0b\u7528 5% BSA \u5c01\u9589 30 \u5206\u9418\u3002\u7d30\u80de\u5728 1:100 \u548c 4\u00b0C \u4e0b\u7528 IR91-384 \u6297 SQSTM1 \/ p62 \u6297\u9ad4\uff08\u7d05\u8272\uff09\u67d3\u8272\u3002DAPI\uff08\u85cd\u8272\uff09\u7528\u4f5c\u6838\u8907\u67d3\u5291\u3002<\/p>\n

\"IR91-384<\/p>\n

\u6297 SQSTM1 \/ p62 \u6297\u9ad4_MDA-MB-231 ICC\/ IF \u5716\u50cf(IREAL, #IR91-384)<\/span><\/strong><\/span>
\n\u514d\u75ab\u7192\u5149\uff1a\u7d30\u80de\u5728\u5ba4\u6eab\u4e0b\u7528 4% \u591a\u805a\u7532\u919b\u56fa\u5b9a 10 \u5206\u9418\uff0c\u5728\u5ba4\u6eab\u4e0b\u7528 0.1% NP-40 \u900f\u5316 10 \u5206\u9418\uff0c\u7136\u5f8c\u5728\u5ba4\u6eab\u4e0b\u7528 5% BSA \u5c01\u9589 30 \u5206\u9418\u3002\u7d30\u80de\u5728 1:100 \u548c 4\u00b0C \u4e0b\u7528 IR91-384 \u6297 SQSTM1 \/ p62 \u6297\u9ad4\uff08\u7d05\u8272\uff09\u67d3\u8272\u3002DAPI\uff08\u85cd\u8272\uff09\u7528\u4f5c\u6838\u8907\u67d3\u5291\u3002<\/p>\n

\"IR91-384<\/p>\n

\u6297 SQSTM1 \/ p62 \u6297\u9ad4_HUVEC ICC\/ IF \u5716\u50cf(IREAL, #IR91-384)<\/span><\/strong><\/span>
\n\u514d\u75ab\u7192\u5149\uff1a\u7d30\u80de\u5728\u5ba4\u6eab\u4e0b\u7528 4% \u591a\u805a\u7532\u919b\u56fa\u5b9a 10 \u5206\u9418\uff0c\u5728\u5ba4\u6eab\u4e0b\u7528 0.1% NP-40 \u900f\u5316 10 \u5206\u9418\uff0c\u7136\u5f8c\u5728\u5ba4\u6eab\u4e0b\u7528 5% BSA \u5c01\u9589 30 \u5206\u9418\u3002\u7d30\u80de\u5728 1:100 \u548c 4\u00b0C \u4e0b\u7528 IR91-384 \u6297 SQSTM1 \/ p62 \u6297\u9ad4\uff08\u7d05\u8272\uff09\u67d3\u8272\u3002DAPI\uff08\u85cd\u8272\uff09\u7528\u4f5c\u6838\u8907\u67d3\u5291\u3002<\/p>\n\n\n\n\n\n\n\n
IR300-981<\/td>\nCD9<\/a><\/span><\/td>\nExosome Biomarker<\/td>\nRabbit<\/td>\nH,M,R<\/td>\nWB, IF, IHC<\/td>\n<\/tr>\n
IR301-983<\/td>\nCD63<\/a><\/span><\/td>\nExosome Biomarker<\/td>\nRabbit<\/td>\nH,M,R<\/td>\nWB, IHC, FC<\/td>\n<\/tr>\n
IR302-986<\/td>\nCD81<\/a><\/span><\/td>\nExosome Biomarker<\/td>\nRabbit<\/td>\nH,M,R<\/td>\nWB, IF, IHC<\/td>\n<\/tr>\n
IR304-990<\/td>\nFlotillin-1<\/td>\nExosome Biomarker<\/td>\nRabbit<\/td>\nH,M,R<\/td>\nWB, IF, IHC<\/td>\n<\/tr>\n
IR303-988<\/td>\nALIX\u6297\u9ad4<\/a><\/span><\/td>\nExosome Biomarker<\/td>\nRabbit<\/td>\nH,M,R<\/td>\nWB, IF, IHC<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\"<\/p>\n

IHC\/\u75c5\u7406\u5b78\u6297\u9ad4(IHC\/Pathology antibodies)<\/span><\/strong><\/span><\/p>\n

Please note that related Positive Control Slides are also available. \u5206\u5b50\u75c5\u7406\u5b78(molecular pathology)\u4e2d\u7684\u514d\u75ab\u7d44\u7e54\u5316\u5b78(Immunohistochemistry)\u626e\u6f14\u91cd\u8981\u4f5c\u7528\uff1a\u8a3a\u65b7(Diagnosis)\u3001\u9810\u5f8c(Prognosis)\u548c\u9810\u6e2c(Predictive)\u3002 IHC \u6297\u9ad4(IHC antibodies)\u5df2\u88ab\u78ba\u5b9a\u70ba\u7528\u65bc\u798f\u723e\u99ac\u6797\u56fa\u5b9a\u77f3\u881f\u5207\u7247(formalin-fixed paraffin sections)\u514d\u75ab\u7d44\u7e54\u5316\u5b78(immunohistochemistry)\u7684\u6700\u4f73\u8a66\u5291\u3002\u6240\u6709 IHC\/\u75c5\u7406\u5b78\u6297\u9ad4(IHC\/Pathology antibodies)\u50c5\u4f9b\u7814\u7a76\u4f7f\u7528\u3002IHC\/\u75c5\u7406\u5b78\u6297\u9ad4\u5df2\u7d93\u91dd\u5c0d\u4eba\u9ad4\/\u8001\u9f20\/\u5927\u9f20\u7d44\u7e54\u7684\u514d\u75ab\u7d44\u7e54\u5316\u5b78\u9032\u884c\u4e86\u5ee3\u6cdb\u7684\u6e2c\u8a66\u548c\u9a57\u8b49(Pathology validated)\uff0c\u4e26\u7d93\u904e\u7cbe\u5fc3\u8a2d\u8a08\uff0c\u53ef\u63d0\u4f9b\u4e00\u81f4\u3001\u7279\u7570\u6027\u548c\u654f\u611f\u7684\u67d3\u8272\u3002<\/p>\n

PD-1\u75c5\u7406\u5b78\u6297\u9ad4\uff1a<\/span>\u7a0b\u5e8f\u6027\u6b7b\u4ea1 1 (Programmed Death 1, PD-1) \u662f T \u7d30\u80de\u8abf\u7bc0\u5291(T-cell regulators) CD28\/CTLA-4 \u5bb6\u65cf\u7684\u6210\u54e1\uff0c\u5728\u6d3b\u5316\u7684 T \u7d30\u80de\u3001B \u7d30\u80de\u548c\u5de8\u566c\u7d30\u80de\u8868\u9762\u8868\u9054\u70ba\u5171\u53d7\u9ad4(co-receptor)\u3002\u65b0\u7684\u7814\u7a76\u8868\u660e\uff0cPD-1\/PD-L1 \u4fe1\u865f\u901a\u8def\u53ef\u80fd\u8207\u6297\u816b\u7624\u514d\u75ab\u6709\u95dc\uff0c\u56e0\u70ba PD-L1 \u5df2\u88ab\u8b49\u660e\u53ef\u8a98\u5c0e\u6d3b\u5316 T \u7d30\u80de\u51cb\u4ea1(apoptosis)\u6216\u6291\u5236\u7d30\u80de\u6bd2\u6027 T \u7d30\u80de(cytotoxic T cells)\u7684\u6d3b\u6027\u3002\u8207 CD10 \u548c BCL6 \u4e0d\u540c\uff0cPD-1 \u50c5\u7531\u5c11\u6578 B \u7d30\u80de\u8868\u9054\uff0c\u56e0\u6b64\u6297 PD-1 \u53ef\u80fd\u662f\u8840\u7ba1\u514d\u75ab\u6bcd\u7d30\u80de\u6027\u6dcb\u5df4\u7624(angioimmunoblastic lymphoma)\u4e2d\u66f4\u7279\u7570\u548c\u6709\u7528\u7684\u8a3a\u65b7\u6a19\u8a8c\u7269\u3002>>\u00a0PD-L1\/CD274 C\u672b\u7aef\u6297\u9ad4<\/a><\/span><\/p>\n

CTLA4\u75c5\u7406\u5b78\u6297\u9ad4\uff1a<\/span>CD152 \u6297\u539f\uff0c\u4e5f\u7a31\u70ba CTLA4\uff0c\u662f\u4e00\u7a2e\u5c6c\u65bc\u514d\u75ab\u7403\u86cb\u767d\u8d85\u5bb6\u65cf(immunoglobulin superfamily)\u7684\u5b8c\u6574\u819c\u86cb\u767d\uff0c\u5176\u8868\u9054\u70ba 30 kDa \u55ae\u9ad4(30 kDa monomer)\u6216\u4e8c\u786b\u9375\u9023\u63a5\u7684\u540c\u578b\u4e8c\u805a\u9ad4(disulfide-linked homodimer)\u3002\u5b83\u8207T\u7d30\u80de\u6297\u539fCD28(T cell antigen CD28)\u5177\u6709\u540c\u6e90\u6027\uff0c\u8207CD28\u4e00\u6a23\uff0c\u662fCD80\u548cCD86\u7684\u914d\u9ad4(ligand)\u3002 CTLA-4 \u662f T \u8f14\u52a9\u7d30\u80de\u4e0a\u7684\u4e00\u7a2e\u53d7\u9ad4\uff0c\u53ef\u4f5c\u70ba\u514d\u75ab\u6aa2\u67e5\u9ede(immune checkpoint)\u548c\u514d\u75ab\u53cd\u61c9\u7684\u4e0b\u8abf\u56e0\u5b50\u3002 CTLA-4 \u7684\u7a81\u8b8a\u8207\u8a31\u591a\u81ea\u8eab\u514d\u75ab\u6027\u75be\u75c5(autoimmune diseases)\u6709\u95dc\u3002<\/p>\n

Lp-PLA2\u75c5\u7406\u5b78\u6297\u9ad4\uff1a<\/span>Lp-PLA2\uff0c\u4e5f\u7a31\u70ba\u8102\u86cb\u767d\u76f8\u95dc\u78f7\u8102\u9176 A2 (Lipoprotein-associated Phospholipase A2)\u6216\u8840\u5c0f\u677f\u6d3b\u5316\u56e0\u5b50\u4e59\u9170\u6c34\u89e3\u9176 (platelet-activating factor acetylhydrolase , PAF-AH)\uff0c\u662f\u4e00\u7a2e\u7531\u708e\u75c7\u7d30\u80de(inflammatory cells )\u7522\u751f\u7684\u9176\uff0c\u5176\u529f\u80fd\u662f\u4f7f\u8840\u5c0f\u677f\u6d3b\u5316\u56e0\u5b50(platelet-activating factor)\u5931\u6d3b\u548c\u964d\u89e3(degrade)\uff0c\u8840\u5c0f\u677f\u6d3b\u5316\u56e0\u5b50\u662f\u4e00\u7a2e\u6709\u6548\u7684\u4fc3\u708e\u6027\u78f7\u8102(pro-inflammatory phospholipid)\uff0c\u540c\u6642\u9084\u8ca0\u8cac\u6c34\u89e3\u6c27\u5316\u4fee\u98fe(hydrolyzing oxidatively modified)\u7684\u591a\u4e0d\u98fd\u548c\u8102\u80aa\u9178(polyunsaturated fatty acids)\u3002 Lp-PLA2 \u7684\u9176\u4fc3\u4f5c\u7528\u7522\u7269\u8207\u52d5\u8108\u7ca5\u6a23\u786c\u5316(atherosclerosis)\u7684\u767c\u5c55\u6709\u95dc\uff0c\u4e26\u8207\u51a0\u5fc3\u75c5(coronary heart disease)\u548c\u4e2d\u98a8(stroke)\u7684\u98a8\u96aa\u589e\u52a0\u5448\u6b63\u76f8\u95dc\u3002<\/p>\n

Muc2\u75c5\u7406\u5b78\u6297\u9ad4\uff1a<\/span>\u7c98\u86cb\u767d 2(Mucin 2)\uff0c\u4e5f\u7a31\u70ba MUC2\uff0c\u662f\u4e00\u7a2e\u7de8\u78bc\u7c98\u86cb\u767d\u5bb6\u65cf(mucin protein family)\u6210\u54e1\u7684\u4eba\u985e\u57fa\u56e0\u3002\u7531\u8a72\u57fa\u56e0\u7de8\u78bc\u7684\u86cb\u767d\u8cea\u5f62\u6210\u4fdd\u8b77\u8178\u8154(gut lumen)\u7684\u4e0d\u6eb6\u6027\u7c98\u6db2\u5c4f\u969c\u3002\u5728\u6b63\u5e38\u7d50\u8178(normal colon)\u3001\u4e73\u817a(breast)\u3001\u524d\u5217\u817a(prostate)\u548c\u553e\u6db2\u817a(salivary gland)\u7b49\u4eba\u9ad4\u7d44\u7e54\u4ee5\u53ca\u80c3\u8178\u9053(gastrointestinal)\u3001\u7d50\u8178(colonic)\u3001\u4e73\u817a(breast)\u548c\u524d\u5217\u817a\u816b\u7624(prostate neoplasia)\u4e2d\u6aa2\u6e2c\u5230 MUC2 \u8868\u9054\u3002<\/p>\n

\u7d30\u80de\u89d2\u86cb\u767d 18 (Cytokeratin 18) \u75c5\u7406\u5b78\u6297\u9ad4\uff1a<\/span>\u7d30\u80de\u89d2\u86cb\u767d 18 (Cytokeratin 18, CK 18) \u662f\u4e00\u7a2e 45kDa \u7684\u86cb\u767d\u8cea\uff0c\u5c6c\u65bc\u9178\u6027\u985e\u578b\u7684\u7d30\u80de\u89d2\u86cb\u767d(cytokeratins)\uff0c\u901a\u5e38\u5728\u7c21\u55ae\u7684\u975e\u5206\u5c64\u4e0a\u76ae\u7d30\u80de(non-stratified epithelia)\u4e2d\u8868\u9054\u3002\u7136\u800c\uff0cCK 18 \u4e5f\u5728\u79fb\u884c\u4e0a\u76ae(transitional epithelium)\u7684\u57fa\u5e95\u7d30\u80de\u548c\u8868\u5c64\u7d30\u80de\u4ee5\u53ca\u8907\u96dc\u4e0a\u76ae(complex epithelia)\u7684\u7ba1\u8154\/\u5206\u6ccc\u7d30\u80de(luminal\/secretory cells)\u4e2d\u8868\u9054\u3002 CK 18 \u6297\u9ad4\u5728\u8d77\u6e90\u65bc\u7c21\u55ae\u548c\u817a\u9ad4\u4e0a\u76ae(glandular epithelium)\u7684\u817a\u764c(Adenocarcinomas)\u4ee5\u53ca\u9c57\u72c0\u7d30\u80de\u764c(Squamous Carcinoma)\u7684\u4f4e\u5206\u5316\u816b\u7624\u7d30\u80de\u4e2d\u5448\u967d\u6027\u67d3\u8272\u3002<\/p>\n

MLH1\u75c5\u7406\u5b78\u6297\u9ad4\u00a0<\/span>\u662f\u4e00\u7a2e\u932f\u914d\u4fee\u5fa9\u86cb\u767d(mismatch repair protein)\uff0c\u8207 MSH2\u3001MSH6 \u548c PMS2 \u4e00\u8d77\u53c3\u8207\u7dad\u6301\u907a\u50b3\u4fe1\u606f\u7684\u5b8c\u6574\u6027\u3002\u9019\u7a2e\u86cb\u767d\u8cea\u901a\u5e38\u8207\u907a\u50b3\u6027(hereditary)\u975e\u606f\u8089\u75c5\u6027\u7d50\u76f4\u8178\u764c(non-polyposis colorectal cancer)\u6709\u95dc\uff0c\u56e0\u70ba MLH1 \u57fa\u56e0\u5728\u60a3\u6709\u9019\u7a2e\u764c\u75c7\u7684\u60a3\u8005\u4e2d\u7d93\u5e38\u767c\u751f\u7a81\u8b8a\u3002\u7814\u7a76\u8868\u660e\uff0cMLH1 \u5728\u5927\u90e8\u5206\u5fae\u885b\u661f\u4e0d\u7a69\u5b9a\u6027(microsatellite instability)\u4ee5\u53ca\u5b50\u5bae\u5167\u819c\u764c(endometrial)\u548c\u5375\u5de2\u764c(ovarian cancers)\u60a3\u8005\u4e2d\u5b58\u5728\u7f3a\u9677\u3002\u7576\u5728 IHC \u9762\u677f\u4e2d\u8207 MSH6\u3001MSH2 \u548c PMS2 \u914d\u5c0d\u6642\uff0c\u6297 MLH1 \u7684\u4f7f\u7528\u5f97\u5230\u512a\u5316\u3002<\/p>\n

HER2\u75c5\u7406\u5b78\u6297\u9ad4:<\/span><\/p>\n

HER2\u5728 10-20% \u7684\u4e73\u817a\u764c\u60a3\u8005(breast cancer patients)\u4e2d\u88ab\u8b58\u5225\u51fa\u4f86\u3002 Her2\/Neu (c-erbB-2) \u539f\u764c\u57fa\u56e0(proto-oncogene)\u662f\u4e00\u7a2e\u8de8\u819c\u53d7\u9ad4\u916a\u6c28\u9178\u6fc0\u9176(transmembrane receptor tyrosine kinase)\uff0c\u5728\u8a31\u591a\u764c\u75c7\u4e2d\u90fd\u6709\u81e8\u5e8a\u8868\u73fe\u3002 c-erbB-2 \u86cb\u767d\u7684\u904e\u5ea6\u8868\u9054(Overexpression)\u8207\u5c0e\u7ba1\u4e73\u817a\u764c(ductal breast cancer)\u4ee5\u53ca\u80ba\u817a\u764c(pulmonary)\u548c\u80c3\u817a\u764c(gastric adenocarcinomas)\u6709\u95dc\u3002Her2 \u548c p53 \u4e4b\u9593\u7684\u76f8\u95dc\u6027\u4e5f\u5df2\u88ab\u8b49\u660e\uff0c\u56e0\u70ba\u9019\u5169\u7a2e\u86cb\u767d\u7684\u904e\u5ea6\u8868\u9054\u8207\u8180\u80f1\u764c\u65e9\u671f\u4fb5\u8972(early invasion)\u548c\u8f49\u79fb(metastasis)\u6709\u95dc\u3002<\/p>\n

Ki-67\u00a0<\/span>\u662f\u4e00\u7a2e\u6838\u975e\u7d44\u86cb\u767d(nuclear non-histone protein)\uff0c\u5728\u975c\u6b62\u7d30\u80de(quiescent cells)\u4e2d\u4ee5\u4f4e\u6c34\u5e73\u5b58\u5728\uff0c\u4f46\u5728\u589e\u6b96\u7d30\u80de(proliferating cells)\u4e2d\u589e\u52a0\uff0c\u5c24\u5176\u662f\u5728 G2\u3001M \u548c S \u671f\u5f8c\u534a\u671f\u3002\u56e0\u6b64\uff0cKi-67 \u53cd\u61c9\u6027\uff08\u5b9a\u7fa9\u70ba\u901a\u904e IHC \u67d3\u8272\u6e2c\u91cf\u7684\u67d3\u8272\u967d\u6027\u7684\u816b\u7624\u7d30\u80de\u767e\u5206\u6bd4\uff09\u662f\u7d30\u80de\u589e\u6b96(cell proliferation)\u7684\u7279\u7570\u6027\u6838\u6a19\u8a18(nuclear marker )\u3002\u904e\u5ea6\u8868\u9054\u7d93\u5e38\u51fa\u73fe\u5728\u5404\u7a2e\u60e1\u6027\u7d44\u7e54(malignant tissues )\u4e2d\uff0c\u4e26\u4e14\u8207\u60a3\u6709\u795e\u7d93\u5167\u5206\u6ccc\u816b\u7624(neuroendocrine tumor)\u7684\u500b\u9ad4\u7684\u8f03\u5dee\u5b58\u6d3b\u7387\u76f8\u95dc\u3002>>IHC\u75c5\u7406\u5b78\u6297\u9ad4-Ki-67\u00a0\u6297\u9ad4<\/a>\u00a0<\/span><\/strong><\/span><\/p>\n

\"Better<\/p>\n

Exosome \u7814\u7a76\u5de5\u5177\/\u764c\u75c7\u76f8\u95dcExosomal Protein Marker<\/strong><\/span>–IReal Biotechnology<\/span><\/span><\/p>\n

IREAL\u63d0\u4f9b\u591a\u7a2e\u9ad8\u8cea\u91cf\u5916\u6ccc\u9ad4(exosomes)\u55ae\u591a\u682a\u6297\u9ad4 (IREAL Antibody)\uff0c\u5305\u62ecCD9(#IR300-981)<\/a><\/span>\u3001CD63(#IR301-983)<\/a><\/span>\u3001CD81(#IR302-986)<\/a><\/span>\u3001Flotillin-1(#IR304-990)<\/a>\u3001Alix(#IR303-988)<\/a><\/span>\u7b2c\u4e09\u65b9\u9a57\u8b49IHC\/IF\/WB\u6297\u9ad4\u3002\u5916\u6ccc\u9ad4(exosomes)\u53e6\u6709\u63d0\u4f9b\u5957\u7d44\u578b\u5f0f\u7684\u6297\u9ad4Panel\u5957\u7d44<\/a>\u3002<\/span><\/p>\n

\u8b58\u5225\u5916\u6ccc\u9ad4(exosomes)\u7684\u6700\u5e38\u898b\u8de8\u819c\u6a19\u8a18\u4f86\u81ea\u56db\u8de8\u819c\u86cb\u767d\u5bb6\u65cf\uff08CD63 \u3001CD81 \u548c CD9\uff09\u3002\u8a72\u5bb6\u65cf\u4e2d\u7684\u86cb\u767d\u8cea\u5177\u6709\u56db\u500b\u8de8\u819c\u5340\u57df\uff0c\u4e26\u4e14\u5b9a\u4f4d\u65bc\u8cea\u819c\u4e2d\u5bcc\u542b\u56db\u8de8\u819c\u86cb\u767d\u7684\u5fae\u57df (\u00a0tetraspanin enriched microdomains, TEM)\u3002\uff08CD63 \u3001CD81 \u3001 CD9\u53caCD82\uff09\u8207\u7c98\u9644\u5206\u5b50(\u00a0adhesion molecules)\u3001\u8de8\u819c\u53d7\u9ad4(\u00a0transmembrane receptors)\u548c\u7d30\u80de\u5167\u4fe1\u865f\u86cb\u767d(intracellular signaling proteins)\u76f8\u95dc\uff0c\u4ee5\u4fc3\u9032\u548c\u8abf\u7bc0\u4fe1\u865f(\u00a0signaling events)\u4e8b\u4ef6\u3002\u9ad8\u8cea\u91cf\u5916\u6ccc\u9ad4(exosomes)\u55ae\u591a\u682a\u6297\u9ad4 (IREAL Antibody)\uff0c\u63d0\u4f9b\u60a8\u66f4\u591aExosome \u7814\u7a76\u5de5\u5177\uff0c\u53ef\u61c9\u7528\u65bcIHC\/IF\/WB\u6297\u9ad4\u3002<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716<\/p>\n

BRAND\/AGENCY\/<\/strong>\u53f0\u7063\u4ee3\u7406\u5546<\/strong><\/span>\/<\/span>\u00a0<\/a><\/span><\/strong>IREAL<\/strong>\u827e\u92b3\u53f0\u7063\u4ee3\u7406\u5546<\/strong><\/a><\/span><\/span><\/p>\n

IREAL\u827e\u92ed\u5b98\u7db2\u00a0https:\/\/www.irealbio.com\/<\/a><\/span><\/p>\n

IREAL\u827e\u92ed\u751f\u7269\u79d1\u6280\u63d0\u4f9b\u6642\u4e0b\u71b1\u9580\u7814\u7a76\u9818\u57dfEMT marker\u3001Apoptosis\u3001ER stress\u3001Cell Cycle\u3001Autophagy\u53caNeuroscience\u7b49\u5e38\u7528\u6297\u9ad4\uff0c\u4e26\u63d0\u4f9b\u5ba2\u88fd\u5316\u6297\u9ad4\u670d\u52d9\u5e73\u53f0\u3002iReal\u00a0\u827e\u92ed\u751f\u7269\u79d1\u6280\u63d0\u4f9b\u6642\u4e0b\u71b1\u9580\u7814\u7a76\u9818\u57dfEMT marker\u3001Apoptosis\u3001ER stress\u3001Cell Cycle\u3001Autophagy\u53caNeuroscience\u7b49\u5e38\u7528\u6297\u9ad4\uff0c\u4e26\u63d0\u4f9b\u5ba2\u88fd\u5316\u6297\u9ad4\u670d\u52d9\u5e73\u53f0\u3002iReal \u827e\u92b3\u751f\u6280\u7368\u5bb6\u6253\u9020\u51faMonoclonal-like Polyclonal Antibody\uff0c\u514d\u75ab\u539f(Immunogen)\u70ba\u61c9\u7528\u77ed\u93c8\u80dc\u80bd(peptide)\u70ba\u6297\u539f\u8a2d\u8a08\u51fa\u767c\u9ede\uff0c\u6311\u9078\u5404\u86cb\u767d\u6700\u4f73\u7684\u4f4d\u9ede(epitope) \u8fa8\u8b58\u4f4d\u4f86\u8fa8\u8a8d\u76ee\u6a19\u86cb\u767d\uff0ciReal\u00a0\u514d\u75ab\u8840\u6e05\u7d93\u904e\u4e00\u9023\u591a\u6b65\u9a5f\u7684\u7d14\u5316\u904e\u7a0b(Affinity column purified )\uff0c\u53bb\u9664\u6389\u5404\u7a2e\u975e\u5c08\u4e00\u6027\u7684\u86cb\u767d\uff0c\u4ee5\u964d\u4f4e\u6297\u9ad4\u7684\u80cc\u666f\u503c\uff1b\u540c\u6642\u85c9\u7531\u5154\u5b50\u7368\u7279\u7684\u514d\u75ab\u7cfb\u7d71\u6240\u7522\u751f\u7684\u6297\u9ad4\uff0c\u80fd\u5920\u7522\u2f63\u8a31\u591a\u2f7c\u2fcf\u514d\u75ab\u7cfb\u7d71\u4e0d\u80fd\u8b58\u5225\u7684\u6297\u539f\u7684\u6297\u9ad4\u3002\u64c1\u6709\u5404\u5b78\u8853\u55ae\u4f4d\u771f\u5be6\u6578\u64da\u7684\u9a57\u8b49\uff0c\u4e26\u5177\u5099\u81e8\u5e8a\u9a57\u8b49\u898f\u683c\u7684\u6297\u9ad4\u54c1\u8cea\uff0c\u5927\u91cf\u4e14\u73fe\u8ca8\u4f9b\u61c9IHC\/IF\/WB\u6297\u9ad4\u3002<\/p>\n

\"IREAL\u827e\u92b3\u514d\u75ab\u67d3\u8272\u6297\u9ad4-\u592a\u9f0e\u751f\u6280_\u5de5\u4f5c\u5340\u57df<\/p>\n

\"\u578b\u9304\u4e0b\u8f09\"<\/p>\n

\"\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

IHC\/WB\/IF\/IP\u7814\u7a76\u5de5\u5177-Autophagy\u00a0\/ ER stress\u00a0Antibodies&#8211 […]<\/p>\n","protected":false},"author":3,"featured_media":38534,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5264],"tags":[5449],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40013"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=40013"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40013\/revisions"}],"predecessor-version":[{"id":40088,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40013\/revisions\/40088"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/38534"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=40013"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=40013"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=40013"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}