{"id":39881,"date":"2023-03-12T15:01:11","date_gmt":"2023-03-12T07:01:11","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=39881"},"modified":"2023-03-28T11:48:44","modified_gmt":"2023-03-28T03:48:44","slug":"pvnv%e5%8d%97%e7%be%8e%e7%99%bd%e8%9d%a6%e8%ab%be%e9%81%94%e7%97%85%e6%af%92%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%a5%97%e7%b5%84-pockit-micro%e7%b3%bb%e5%88%97%e6%89%8b%e6%8c%81%e5%bc%8f%e6%a0%b8","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=39881","title":{"rendered":"PvNV\u5357\u7f8e\u767d\u8766\u8afe\u9054\u75c5\u6bd2\u6aa2\u6e2c\u8a66\u5957\u7d44 | POCKIT\u2122 Micro\u7cfb\u5217\u624b\u6301\u5f0f\u6838\u9178\u5206\u6790\u5100\/iiPCR \u6c34\u7522\u75c5\u539f\u76f8\u95dc\u6aa2\u6e2c"},"content":{"rendered":"

PvNV\u5357\u7f8e\u767d\u8766\u8afe\u9054\u75c5\u6bd2\u6aa2\u6e2c\u8a66\u5957\u7d44 | POCKIT\u2122 Micro\u7cfb\u5217\u624b\u6301\u5f0f\u6838\u9178\u5206\u6790\u5100\/iiPCR \u6c34\u7522\u75c5\u539f\u76f8\u95dc\u6aa2\u6e2c<\/a><\/span><\/p>\n

IQ Plus \u5357\u7f8e\u767d\u8766\u8afe\u9054\u75c5\u6bd2\u6aa2\u6e2c\u5957\u7d44-\u5357\u7f8e\u767d\u8766\u8afe\u9054\u75c5\u6bd2(Penaeus vannamei nodavirus, PvNV) <\/span><\/p>\n

POCKIT\u2122 Micro\u7cfb\u5217\u624b\u6301\u5f0f\u6838\u9178\u5206\u6790\u5100\u70ba\u6700\u65b0\u4e00\u4ee3PCR\u6aa2\u6e2c\u7cfb\u7d71\uff0c\u5167\u5efa\u5145\u96fb\u5f0f\u96fb\u6c60\uff0c\u5373\u4f7f\u7121\u5be6\u9a57\u5ba4\u64cd\u4f5c\u7d93\u9a57\uff0c\u4ecd\u53ef\u96a8\u6642\u96a8\u5730\u9032\u884c\u6aa2\u6e2c\u3002<\/p>\n

\"\"<\/p>\n

\u66f4\u591a\u6aa2\u6e2c>><\/span>\u00a0PvNV\u5357\u7f8e\u767d\u8766\u8afe\u9054\u75c5\u6bd2\u6aa2\u6e2c<\/a>\u00a0|\u00a0WSSV\u767d\u9ede\u75c7\u75c5\u6bd2\u6aa2\u6e2c<\/a><\/span>\u00a0|\u00a0IMNV\u50b3\u67d3\u6027\u808c\u8089\u58de\u6b7b\u75c5\u6bd2\u6aa2\u6e2c<\/a><\/span><\/p>\n

\u2022 \u904b\u7528\u9694\u7d55\u5f0f\u6046\u6eab\u805a\u5408\u9176\u9023\u9396\u53cd\u61c9(iiPCR)\u6280\u8853\u3002<\/span><\/h3>\n

\u2022 30-45\u5206\u9418\u5167\u5b8c\u6210PCR\u8207\u7d50\u679c\u5224\u8b80\u3002<\/span><\/h3>\n

\u2022 \u55ae\u9375\u5f0f\u64cd\u4f5c\uff0c\u64cd\u4f5c\u7c21\u55ae\u3002<\/span><\/h3>\n

\u2022 \u6aa2\u6e2c\u7d50\u679c\u76f4\u63a5\u986f\u793a\u65bc\u87a2\u5e55\uff0c\u5224\u8b80\u7c21\u55ae\u3002<\/span><\/h3>\n

\u2022 \u5167\u5efa\u5145\u96fb\u5f0f\u92f0\u96fb\u6c60\uff0c\u4e0d\u9700\u63d2\u96fb\u5373\u53ef\u4f7f\u7528\u3002<\/span><\/h3>\n

\"\"<\/p>\n

\u66f4\u591a\u6aa2\u6e2c>>\u00a0 <\/span>TSV\u6843\u62c9\u75c5\u6bd2\u6aa2\u6e2c<\/a>\u00a0|\u00a0YHV\u9ec3\u982d\u75c5\u6bd2\u6aa2\u6e2c<\/a>\u00a0<\/span>| \u54c8\u7dad\u6c0f\u5f27\u83cc\u6aa2\u6e2c<\/a>\u00a0|\u00a0EHP\u8766\u8178\u7d30\u80de\u87f2\u6aa2\u6e2c<\/a><\/span><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
<\/th>\n\n
POCKIT\u2122 Micro<\/div>\n
\u6838\u9178\u5206\u6790\u5100<\/div>\n<\/th>\n
\n
POCKIT\u2122 Micro Plus<\/div>\n
\u6838\u9178\u5206\u6790\u5100<\/div>\n<\/th>\n
\n
POCKIT\u2122 Micro Duo<\/div>\n
\u6838\u9178\u5206\u6790\u5100<\/div>\n<\/th>\n<\/tr>\n
PCR \u64f4\u589e\u6280\u8853<\/span><\/td>\n\u9694\u7d55\u5f0f\u6046\u6eab\u805a\u5408\u9176\u9023\u9396\u53cd\u61c9 (iiPCR)<\/span><\/td>\n\u9694\u7d55\u5f0f\u6046\u6eab\u805a\u5408\u9176\u9023\u9396\u53cd\u61c9 (iiPCR)<\/span><\/td>\n\u9694\u7d55\u5f0f\u6046\u6eab\u805a\u5408\u9176\u9023\u9396\u53cd\u61c9 (iiPCR)<\/span><\/td>\n<\/tr>\n
\u87a2\u5149\u6ce2\u9577<\/span><\/td>\n520 nm<\/span><\/td>\n520 nm<\/span><\/td>\n520 nm \/ 550nm<\/span><\/td>\n<\/tr>\n
\u6aa2\u6e2c\u6a19\u7684<\/span><\/td>\nDNA<\/span><\/td>\nDNA \/ RNA<\/span><\/td>\nDNA \/ RNA<\/span><\/td>\n<\/tr>\n
\u9748\u654f\u5ea6<\/span><\/td>\n\u8207\u87a2\u5149\u5b9a\u91cfPCR(Real-time PCR)\u76f8\u540c<\/span><\/td>\n\u8207\u87a2\u5149\u5b9a\u91cfPCR(Real-time PCR)\u76f8\u540c<\/span><\/td>\n\u8207\u87a2\u5149\u5b9a\u91cfPCR(Real-time PCR)\u76f8\u540c<\/span><\/td>\n<\/tr>\n
\u901a\u91cf<\/span><\/td>\n1 – 4 \u6a23\u672c\/\u6b21<\/span><\/td>\n1 – 4\u6a23\u672c\/\u6b21<\/span><\/td>\n1 – 4 \u6a23\u672c\/\u6b21<\/span><\/td>\n<\/tr>\n
PCR \u53cd\u61c9\u6642\u9593<\/span><\/td>\n\u7d04 30\u5206\u9418<\/span><\/td>\n\u7d04 45\u5206\u9418<\/span><\/td>\n\u7d04 45\u5206\u9418<\/span><\/td>\n<\/tr>\n
\u5132\u5b58\u529f\u80fd<\/span><\/td>\n\u7121<\/span><\/td>\n\u7121<\/span><\/td>\nMicro SD card<\/span><\/td>\n<\/tr>\n
\u5c3a\u5bf8<\/span><\/td>\n15.2 (\u9577) \u2613 6.3 (\u5bec) \u2613 5 (\u9ad8) \u516c\u5206<\/span><\/td>\n15.2 (\u9577) \u2613 6.3 (\u5bec) \u2613 5 (\u9ad8) \u516c\u5206<\/span><\/td>\n16 (\u9577) \u00d7 7.8 (\u5bec) \u00d7 5.5 (\u9ad8) \u516c\u5206<\/span><\/td>\n<\/tr>\n
\u91cd\u91cf<\/span><\/td>\n380 \u514b<\/span><\/td>\n380 \u514b<\/span><\/td>\n430 g<\/span><\/td>\n<\/tr>\n
\u5ba4\u6eab\u8981\u6c42<\/span><\/td>\n15 – 35\u00b0C<\/span><\/td>\n15 – 35\u00b0C<\/span><\/td>\n15 – 35\u00b0C<\/span><\/td>\n<\/tr>\n
\u96fb\u6e90<\/span><\/td>\n\u92f0\u96fb\u6c60<\/span><\/td>\n\u92f0\u96fb\u6c60<\/span><\/td>\n\u92f0\u96fb\u6c60<\/span><\/td>\n<\/tr>\n
\u4fdd\u56fa<\/span><\/td>\n1\u5e74<\/span><\/td>\n1\u5e74<\/span><\/td>\n1 \u5e74<\/span><\/td>\n<\/tr>\n
\u8a8d\u8b49<\/span><\/td>\n–<\/span><\/td>\nTFDA\u00a0<\/span><\/td>\nTFDA\u00a0<\/span><\/td>\n<\/tr>\n
\u5f97\u734e\u69ae\u8000<\/td>\n–<\/td>\n\"\"<\/b><\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\"<\/p>\n

iiPCR\u76f8\u95dc\u6aa2\u6e2c\u8a66\u5291\u5957\u7d44<\/span><\/strong><\/p>\n

iiPCR\u76f8\u95dc\u6aa2\u6e2c\u8a66\u5291\u4f7f\u7528\u9694\u7d55\u5f0f\u6046\u6eabPCR\uff08iiPCR\uff09\u6280\u8853\uff0c\u6aa2\u6e2c\u5fae\u751f\u7269\u75c5\u539f\u9ad4\u7684\u7279\u5b9a\u6838\u9178\u5e8f\u5217\uff0c\u56e0\u9ad8\u9748\u654f\u5ea6\u548c\u5c08\u4e00\u6027\u4e4b\u7279\u6027\uff0c\u53ef\u7528\u65bc\u5728\u65e9\u671f\u6216\u4e9e\u81e8\u5e8a\u611f\u67d3\u6aa2\u6e2c\uff0c\u907f\u514d\u611f\u67d3\u8513\u5ef6\uff1b\u70ba\u6709\u6548\u7684\u52d5\u7269\u75ab\u60c5\u76e3\u6e2c\u6a5f\u5236\u3002<\/p>\n

\u7279\u8272<\/span><\/h3>\n

\u2022 \u9748\u654f\u5ea6\u8207\u5c08\u4e00\u6027\u9ad8<\/p>\n

\u2022 \u65b9\u4fbf\u4e14\u7a69\u5b9a\u7684\u51cd\u4e7e\u8a66\u5291\u5305\u88dd<\/p>\n

iiPCR\u6aa2\u6e2c\u8a66\u5291\u5957\u7d44\u4e00\u89bd\u8868<\/a>\u00a0<\/span><\/strong><\/p>\n\n\n\n\n\n\n
<\/td>\nPOCKIT\u2122 \u7cfb\u5217<\/td>\nPOCKIT\u2122 Central \u7cfb\u5217<\/td>\n<\/tr>\n
\u5305\u88dd
\n\u898f\u683c<\/td>\n		48 \u53cd\u61c9\/\u7d44<\/td>\n24 \u53cd\u61c9\/\u7d44<\/td>\n<\/tr>\n
\u9748\u654f\u5ea6<\/td>\n\u7d0410 copies\/\u53cd\u61c9<\/td>\n\u7d0410 copies\/\u53cd\u61c9<\/td>\n<\/tr>\n
\u9069\u7528PCR
\n\u5100\u5668<\/td>\n		\n

\uffed POCKIT\u2122 \u6838\u9178\u5206\u6790\u5100<\/a><\/p>\n

\uffed POCKIT\u2122 Micro \u7cfb\u5217\u624b\u6301\u5f0f\u6838\u9178\u5206\u6790\u5100<\/a><\/td>\n

POCKIT\u2122 Central \u81ea\u52d5\u5316\u6838\u9178\u5206\u6790\u5100<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0iiPCR<\/a>\u00a0POCKIT \u9b5a\u985e\u75be\u75c5\u76f8\u95dc\u6aa2\u6e2c\u8a66\u5291-Genereach<\/span><\/strong><\/span><\/p>\n

\"\"<\/p>\n

\u9bad\u7522\u6c23\u55ae\u80de\u83cc (Aeromonas salmonicida)
\n\u9bc9\u9b5a\u6c34\u816b\u75c5\u6bd2 (CEV)
\n\u55dc\u51b7\u67f1\u72c0\u687f\u83cc (Flavobacterium psychrophilum)
\n\u8679\u5f69\u75c5\u6bd2-Megalocytivirus (Irido-M)
\n\u8679\u5f69\u75c5\u6bd2-Ranaviruses (Irido-RG)
\n\u50b3\u67d3\u6027\u9bad\u985e\u8ca7\u8840 (ISAV)
\n\u9326\u9bc9\u76b0\u75b9\u75c5\u6bd2 (KHV)
\n\u9b5a\u93c8\u7403\u83cc (Streptococcus iniae)
\n\u7121\u4e73\u93c8\u7403\u83cc (Streptococcus agalactiae)
\n\u9bc9\u9b5a\u6625\u5b63\u6bd2\u8840\u75c7\u75c5\u6bd2 (SVCV)
\n\u5433\u90ed\u9b5a\u6e56\u6cca\u75c5\u6bd2 (TiLV)
\n\u75c5\u6bd2\u6027\u51fa\u8840\u6027\u6557\u8840\u75c7\u75c5\u6bd2 (VHSV)
\n\u6eb6\u85fb\u5f27\u83cc (Vibrio alginolyticus)
\n\u75c5\u6bd2\u6027\u795e\u7d93\u58de\u6b7b\u75c7 (VNN)<\/p>\n

>>iiPCR\u6aa2\u6e2c\u8a66\u5291\u5957\u7d44\u4e00\u89bd\u8868<\/a>>><\/span><\/strong><\/span><\/p><\/blockquote>\n

\"\"<\/p>\n

IQ Plus \u5357\u7f8e\u767d\u8766\u8afe\u9054\u75c5\u6bd2\u6aa2\u6e2c\u5957\u7d44-\u5357\u7f8e\u767d\u8766\u8afe\u9054\u75c5\u6bd2(Penaeus vannamei nodavirus, PvNV)\u00a0<\/span><\/strong><\/p>\n

\u5357\u7f8e\u767d\u8766\u8afe\u9054\u75c5\u6bd2 (Penaeus vannamei nodavirus, PvNV) \u6700\u65e9\u65bc2004\u5e74\u5728Belize\u7684\u5357\u7f8e\u767d\u8766\u990a\u6b96\u5834 (Penaeus vannamei) \u4e2d\u767c\u73fe\uff0c\u7576\u6642\u53d7\u611f\u67d3\u7684\u8766\u5b50\u5c3e\u90e8\u808c\u8089\u7d44\u7e54\u5448\u73fe\u767d\u8272\u4e0d\u900f\u660e\u7684\u808c\u8089\u75c5\u8b8a\u75c7\u72c0\uff0c\u7531 Dr. Kathy F. J. Tang \u7814\u7a76\u8abf\u67e5\u5f8c\u8b49\u5be6\u5357\u7f8e\u767d\u8766\u611f\u67d3\u4e86\u8afe\u9054\u75c5\u6bd2 (nodavirus)\u3002\u5118\u7ba1\u8766\u96bb\u611f\u67d3 PvNV \u5f8c\u7684\u6b7b\u4ea1\u7387\u4e0d\u9ad8\uff0c\u4f46\u4ecd\u7136\u9020\u6210\u4e86\u990a\u6b96\u696d\u8005\u56b4\u91cd\u7684\u7684\u7522\u91cf\u4e0b\u964d (\u611f\u67d3\u5340\u57df\u56b4\u91cd\u8005\u53ef\u6e1b\u5c11\u8fd15\u6210\u7522\u91cf)\u3002\u76ee\u524d\u5c0d\u6b64\u75be\u75c5\u7684\u7814\u7a76\u8207\u4e86\u89e3\u6709\u9650\uff0c\u75c5\u6bd2\u50b3\u64ad\u9014\u5f91\u5c1a\u4e0d\u6e05\u695a\uff0c\u50c5\u5f97\u77e5 P. vannamei \u548c P. monodon \u6700\u5bb9\u6613\u53d7\u5230 PvNV \u7684\u611f\u67d3\u3002\u73fe\u4eca PCR \u6280\u8853\u5df2\u666e\u904d\u7684\u4f7f\u7528\u5728\u6c34\u7522\u990a\u6b96\u7522\u696d\u4e2d\uff0c\u4f5c\u70ba\u75be\u75c5\u8a3a\u65b7\u8207\u9810\u9632\u7684\u65b9\u6cd5\u3002\u4f46\u5efa\u69cb\u4e00\u500b PCR \u5be6\u9a57\u5ba4\u4e0d\u4f46\u6602\u8cb4\u4e14\u9700\u8981\u5c08\u696d\u7684\u6280\u8853\u4eba\u54e1\uff0c\u9019\u6a23\u7684\u9650\u5236\u4f7f PCR \u6aa2\u6e2c\u88ab\u4fb7\u9650\u5728\u8f03\u5927\u7684\u5be6\u9a57\u5ba4\u4e2d\u3002\u70ba\u4e86\u4f7f\u73fe\u5834\u6aa2\u6e2c\u5f97\u4ee5\u5be6\u73fe\uff0cIQ Plus \u7cfb\u7d71\u4f7f\u7528\u4e00\u7a2e\u6700\u65b0\u7684\u6280\u8853 – insulated isothermal PCR (iiPCR) \u4f86\u5075\u6e2c\u75c5\u539f\u9ad4\u3002\u6b64\u65b0\u6280\u8853\u53ef\u85c9\u7531\u4e00\u500b\u5c0f\u5de7\u4e14\u53ef\u651c\u5f0f\u7684\u5100\u5668\u5728\u4e00\u5c0f\u6642\u5167\u5b8c\u6210 PvNV \u7684\u6aa2\u6e2c\u3002\u5176\u8403\u53d6\u6b65\u9a5f\u5df2\u7c21\u5316\u6210\u6bcf\u500b\u4eba\u90fd\u80fd\u8f15\u6613\u64cd\u4f5c\u7684\u65b9\u6cd5\u3002IQ Plus \u7cfb\u7d71\u5c07\u6210\u70ba\u96a8\u6642\u96a8\u5730\u6aa2\u6e2c\u8207\u9810\u9632\u6c34\u7522\u75c5\u539f\u7684\u6709\u6548\u5de5\u5177\u3002<\/p>\n

 <\/p>\n

\u7522\u54c1\u7279\u8272:<\/strong><\/span><\/p>\n