{"id":39723,"date":"2023-03-09T13:51:30","date_gmt":"2023-03-09T05:51:30","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=39723"},"modified":"2023-03-28T11:57:10","modified_gmt":"2023-03-28T03:57:10","slug":"ldh%e4%b9%b3%e9%85%b8%e8%84%ab%e6%b0%ab%e9%85%b6%e6%aa%a2%e6%b8%ac-%e8%9e%a2%e5%85%89-%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fabcam-ab197004-k314-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=39723","title":{"rendered":"LDH\u4e73\u9178\u812b\u6c2b\u9176\u6aa2\u6e2c (\u87a2\u5149) \u8a66\u5291\u76d2 | \u8ca8\u865fAbcam ab197004 (K314-500)"},"content":{"rendered":"

\u00a0LDH\u4e73\u9178\u812b\u6c2b\u9176\u6aa2\u6e2c (\u87a2\u5149) \u8a66\u5291\u76d2 | \u8ca8\u865fK314-500<\/a>\u00a0 (Abcam ab197004)<\/strong><\/span><\/p>\n

\u7522\u54c1\u63cf\u8ff0:<\/strong>\u00a0\u4e73\u9178\u812b\u6c2b\u9176\uff08LDH\uff0cEC 1.1.1.27\uff09\u662f\u4e00\u7a2e\u7a69\u5b9a \u7684\u9176\uff0c\u5b58\u5728\u65bc\u6240\u6709\u7d30\u80de\u985e\u578b\u4e2d\u3002\u7d30\u80de\u640d\u50b7\u5c0e\u81f4LDH\u9176\u91cb\u653e\u5230\u57f9\u990a\u57fa\u4e2d\uff0c\u5176\u6d3b\u6027\u88ab\u5ee3\u6cdb\u7528\u4f5c\u7d30\u80de\u6bd2\u6027\u7684\u6a19\u8a8c\u7269\u3002\u5b83\u53ef\u9006\u5730\u5c07\u4e73\u9178\u8f49\u5316\u70ba\u4e19\u916e\u9178\uff0c\u4f34\u96a8\u8457NADH\u548cNAD +\u7684\u76f8\u4e92\u8f49\u5316\u3002 BioVision\u7684PicoProbe\u2122LDH\u7d30\u80de\u6bd2\u6027\u7192\u5149\u6e2c\u5b9a\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u9748\u654f\uff0c\u5feb\u901f\uff0c\u7c21\u4fbf\u7684\u65b9\u6cd5\u4f86\u6aa2\u6e2c\u5f9e\u53d7\u640d\u7d30\u80de\u91cb\u653e\u7684LDH\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0cLDH\u5c07\u4e73\u9178\u8f49\u5316\u70ba\u4e19\u916e\u9178\u548cNADH\uff0c\u5f9e\u800c\u5c07\u5c08\u6709\u63a2\u91dd\u9084\u539f\u70ba\u5f37\u87a2\u5149\u7522\u7269\uff08Ex \/ Em = 535\/587 nm\uff09\u3002\u87a2\u5149\u91cf\u8207\u53d7\u640d\u7d30\u80de\u7684\u6578\u91cf\u6210\u6b63\u6bd4\u3002\u8a72\u6e2c\u5b9a\u6cd5\u9069\u7528\u65bc\u9ad8\u901a\u91cf\u5f62\u5f0f\uff0c\u53ef\u4ee5\u5728\u4e0d\u523020\u5206\u9418\u7684\u6642\u9593\u5167\u5b8c\u6210\u3002\u9748\u654f\u5ea6\uff1a\u301c100\u500b\u7d30\u80de\u3002<\/p>\n

\"\"<\/p>\n

\u672a\u7d93\u8655\u7406\u7684 Jurkat \u7d30\u80de\u6216\u7d93\u7d30\u80de\u88c2\u89e3\u6db2\u8655\u7406\u7684\u7d30\u80de\u3002<\/strong><\/span><\/div>\n
\n

\u672a\u7d93\u8655\u7406\u7684 Jurkat \u7d30\u80de\uff08\u4f4e\u5c0d\u7167\uff09\u6216\u7528\u7d30\u80de\u88c2\u89e3\u6db2\u8655\u7406 30 \u5206\u9418\uff08\u9ad8\u5c0d\u7167\uff09\u3002<\/p>\n

\"\"<\/p>\n

\u672a\u7d93\u8655\u7406\u6216\u7d93\u7d30\u80de\u88c2\u89e3\u6db2\u8655\u7406\u7684 Jurkat \u7d30\u80de\u7684\u76f8\u5c0d\u87a2\u5149\u55ae\u4f4d\u3002<\/strong><\/span><\/div>\n
\n

\u672a\u7d93\u8655\u7406\u7684 Jurkat \u7d30\u80de\uff08\u4f4e\u5c0d\u7167\uff09\u6216\u7528\u7d30\u80de\u88c2\u89e3\u6db2\u8655\u7406 30 \u5206\u9418\uff08\u9ad8\u5c0d\u7167\uff09\u7684\u76f8\u5c0d\u87a2\u5149\u55ae\u4f4d\u3002<\/p>\n

\"\"<\/p>\n

\u7528\u661f\u5f62\u5b62\u83cc\u7d20\u6216\u74b0\u5df1\u9170\u4e9e\u80fa\u904e\u591c\u8655\u7406 HeLa \u7d30\u80de\u3002<\/strong><\/span><\/div>\n
\n

\u7528 100\u03bcM \u661f\u5f62\u5b62\u83cc\u7d20\u6216 3\u03bcM \u74b0\u5df1\u9170\u4e9e\u80fa\u904e\u591c\u8655\u7406 HeLa \u7d30\u80de\u3002\u91cb\u653e\u5230\u57f9\u990a\u57fa\u4e2d\u7684 LDH \u8207\u7a7a\u767d\u3001\u672a\u8655\u7406\u7684\u7d30\u80de\uff08\u4f4e\u5c0d\u7167\uff09\u3001LDH \u967d\u6027\u5c0d\u7167\u548c\u88c2\u89e3\u7d30\u80de\uff08\u9ad8\u5c0d\u7167\uff09\u4e00\u8d77\u6e2c\u91cf<\/p>\n<\/div>\n<\/div>\n<\/div>\n

\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/a><\/p>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a>\u00a0| \u00a0FRAP\u6aa2\u6e2c<\/a>\u00a0|\u00a0\u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a>\u00a0|\u00a0Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a>\u00a0|\u00a0\u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a>\u00a0|\u00a0Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a>\u00a0|\u00a0\u8102\u8cea\u904e\u6c27\u5316\uff08<\/strong>MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a>\u00a0|\u00a0<\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a>\u00a0BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK314-500(Abcam ab197004)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n500 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFluorescence (Ex\/Em = 535\/587 nm)<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nMeasurement of released LDH in culture medium
\nEvaluation of cytotoxicity effect of test compounds<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Highly-Sensitive: Detects as less as 100 cells.
\n\u2022 Rapid protocol: requires less than 20 min.
\n\u2022 High-throughput adaptable.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 LDH Assay Buffer
\n\u2022 LDH Substrate Mix
\n\u2022 PicoProbe\u2122
\n\u2022 Cell Lysis Solution
\n\u2022 LDH Positive Control<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/strong><\/a>\u00a0|\u00a0\u00a0<\/strong>Finnpipette F3\u00a0<\/strong>\u5fae\u91cf\u5206\u6ce8\u5668<\/strong><\/a>\u00a0| \u00a0<\/strong>\u8a66\u7ba1\u632f\u76ea\u5668 Vortex Mixer\u00a0<\/strong><\/a>\u00a0|\u00a0\u00a0<\/strong>Genereach<\/strong>\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/strong><\/a>\u00a0 |\u00a0\u00a0\u00a0<\/strong>ELISA<\/strong>\u5100\u5668<\/strong><\/a><\/span><\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u00a0LDH\u4e73\u9178\u812b\u6c2b\u9176\u6aa2\u6e2c (\u87a2\u5149) \u8a66\u5291\u76d2 | \u8ca8\u865fK314-500\u00a0 (Abcam ab197004) \u7522\u54c1\u63cf […]<\/p>\n","protected":false},"author":3,"featured_media":37625,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261],"tags":[5485],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/39723"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=39723"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/39723\/revisions"}],"predecessor-version":[{"id":39726,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/39723\/revisions\/39726"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/37625"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=39723"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=39723"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=39723"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}