{"id":39656,"date":"2023-03-08T14:35:56","date_gmt":"2023-03-08T06:35:56","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=39656"},"modified":"2023-03-28T11:59:42","modified_gmt":"2023-03-28T03:59:42","slug":"%e7%ba%96%e9%80%a3%e8%9b%8b%e7%99%bd%ef%bc%88c-term%ef%bc%89%e6%8a%97%e9%ab%94-fibronectin-c-term-antibody%e8%b2%a8%e8%99%9fir48-138-ireal-biotechnology-%e8%89%be%e9%8b%ad%e7%94%9f%e6%8a%80","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=39656","title":{"rendered":"\u7e96\u9023\u86cb\u767d\uff08C-term\uff09\u6297\u9ad4 | Fibronectin (C-term) antibody\u8ca8\u865fIR48-138 (IReal Biotechnology \u827e\u92ed\u751f\u6280)"},"content":{"rendered":"

\"HOT\"<\/a>\u7e96\u9023\u86cb\u767d\uff08C-term\uff09\u6297\u9ad4 | Fibronectin (C-term) antibody\u8ca8\u865fIR48-138 (IReal Biotechnology \u827e\u92ed\u751f\u6280)<\/a><\/span><\/span><\/strong><\/p>\n

Fibronectin (C-term) antibody (IReal Biotechnology \u827e\u92ed\u751f\u6280,IR48-138)<\/span><\/p>\n

\u7e96\u9023\u86cb\u767d(Fibronectin)\uff1aFFPE \u4eba\u809d\u7d30\u80de\u764c (human hepatocellular carcinoma, HCC) \u4e2d\u7684\u7d44\u7e54\u6a21\u5f0f\u3002\u9023\u86cb\u767d\u6297\u9ad4(Fibronectin antibody Fibronectin,\u00a0IR48-138 ) \u7e96\u9023\u86cb\u767d\u662f\u7d30\u80de\u5916\u57fa\u8cea(extracellular matrix)\u7684\u4e00\u7a2e\u9ad8\u5206\u5b50\u91cf\u7cd6\u86cb\u767d\u6210\u5206\uff0c\u5728\u7d30\u80de\u5916\u548c\u7d30\u80de\u5167\u5b9a\u4f4d\u65bc\u809d\u7d30\u80de\u764c (HCC) \u7684\u7d30\u80de\u6838(nuclei)\u548c\u7d30\u80de\u8cea(cytoplasm)\u4e2d\u3002\u6838\u7e96\u9023\u86cb\u767d(Nuclear fibronectin)\u53ef\u80fd\u662f\u7e96\u9023\u86cb\u767d DNA \u7d50\u5408\u80fd\u529b\u7684\u4e00\u7a2e\u8868\u9054\uff0c\u5176\u5728\u819c\u901a\u900f\u6027(membrane permeability)\u589e\u52a0\u7684\u7d30\u80de\u4e2d\u8b8a\u5f97\u53ef\u8b49\u5be6\u3002<\/p>\n

iReal \u827e\u92b3\u751f\u6280\u7368\u5bb6\u6253\u9020\u51faMonoclonal-like Polyclonal Antibody\uff0c\u514d\u75ab\u539f(Immunogen)\u70ba\u61c9\u7528\u77ed\u93c8\u80dc\u80bd(peptide)\u70ba\u6297\u539f\u8a2d\u8a08\u51fa\u767c\u9ede\uff0c\u6311\u9078\u5404\u86cb\u767d\u6700\u4f73\u7684\u4f4d\u9ede(epitope) \u8fa8\u8b58\u4f4d\u4f86\u8fa8\u8a8d\u76ee\u6a19\u86cb\u767d\uff0ciReal\u00a0\u514d\u75ab\u8840\u6e05\u7d93\u904e\u4e00\u9023\u591a\u6b65\u9a5f\u7684\u7d14\u5316\u904e\u7a0b(Affinity column purified )\uff0c\u53bb\u9664\u6389\u5404\u7a2e\u975e\u5c08\u4e00\u6027\u7684\u86cb\u767d\uff0c\u4ee5\u964d\u4f4e\u6297\u9ad4\u7684\u80cc\u666f\u503c\uff1b\u540c\u6642\u85c9\u7531\u5154\u5b50\u7368\u7279\u7684\u514d\u75ab\u7cfb\u7d71\u6240\u7522\u751f\u7684\u6297\u9ad4\uff0c\u80fd\u5920\u7522\u2f63\u8a31\u591a\u2f7c\u2fcf\u514d\u75ab\u7cfb\u7d71\u4e0d\u80fd\u8b58\u5225\u7684\u6297\u539f\u7684\u6297\u9ad4\u3002\u64c1\u6709\u5404\u5b78\u8853\u55ae\u4f4d\u771f\u5be6\u6578\u64da\u7684\u9a57\u8b49\uff0c\u4e26\u5177\u5099\u81e8\u5e8a\u9a57\u8b49\u898f\u683c\u7684\u6297\u9ad4\u54c1\u8cea\uff0c\u5927\u91cf\u4e14\u73fe\u8ca8\u4f9b\u61c9\u3002<\/p>\n

\"IR48-138<\/p>\n

Fibronectin (C-term) antibody\u00a0<\/span>(Endogenous)\uff0c\u91dd\u5c0dFibronectin (C-term)\u5177\u6709\u5c08\u4e00\u8868\u73fe\uff0c\u53ef\u61c9\u7528\u65bcIHC(Paraffin)\u3001IF\u3001Western Blot \u5be6\u9a57\uff0c\u7269\u7a2e\u4f86\u6e90\u70baRabbit-IgG\uff0c\u53cd\u61c9\u7269\u7a2e: Human\u3001Mouse\u3001Rat\u3002<\/p>\n

Weatern Blot\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/p>\n

Western Blot 1:500
\nImmunofluorescence 1:200\u20131:500
\nImmunohistochemistry (Paraffin) 1:100\u20131:200<\/p>\n

\"\"<\/p>\n

\u7e96\u9023\u86cb\u767d\uff08C-term\uff09\u6297\u9ad4 Fibronectin (C-term) antibody\u5916\u6ccc\u9ad4\u6297\u9ad4\u7522\u54c1\u4ecb\u7d39:<\/span><\/strong><\/span><\/p>\n

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]<\/p>\n

\u8a72\u57fa\u56e0\u7de8\u78bc\u7e96\u9023\u86cb\u767d\uff0c\u9019\u662f\u4e00\u7a2e\u7cd6\u86cb\u767d\uff0c\u5728\u8840\u6f3f\u4e2d\u4ee5\u53ef\u6eb6\u6027\u4e8c\u805a\u9ad4\u5f62\u5f0f\u5b58\u5728\uff0c\u5728\u7d30\u80de\u8868\u9762\u548c\u7d30\u80de\u5916\u57fa\u8cea\u4e2d\u4ee5\u4e8c\u805a\u9ad4\u6216\u591a\u805a\u9ad4\u5f62\u5f0f\u5b58\u5728\u3002\u7de8\u78bc\u7684\u524d\u539f\u86cb\u767d\u7d93\u904e\u86cb\u767d\u6c34\u89e3\u8655\u7406\u4ee5\u751f\u6210\u6210\u719f\u86cb\u767d\u3002\u7e96\u9023\u86cb\u767d\u53c3\u8207\u7d30\u80de\u7c98\u9644\u548c\u9077\u79fb\u904e\u7a0b\uff0c\u5305\u62ec\u80da\u80ce\u9aee\u751f\u3001\u50b7\u53e3\u7652\u5408\u3001\u8840\u6db2\u51dd\u56fa\u3001\u5bbf\u4e3b\u9632\u79a6\u548c\u8f49\u79fb\u3002\u8a72\u57fa\u56e0\u5177\u6709\u4e09\u500b\u53ef\u9078\u64c7\u6027\u526a\u63a5\u7684\u5340\u57df\uff0c\u6709\u53ef\u80fd\u7522\u751f 20 \u7a2e\u4e0d\u540c\u7684\u8f49\u9304\u672c\u8b8a\u9ad4\uff0c\u5176\u4e2d\u81f3\u5c11\u6709\u4e00\u500b\u7de8\u78bc\u4e00\u7a2e\u53ef\u9032\u884c\u86cb\u767d\u6c34\u89e3\u52a0\u5de5\u7684\u4e9e\u578b\u3002\u4e00\u4e9b\u8b8a\u9ad4\u7684\u5168\u9577\u6027\u8cea\u5c1a\u672a\u78ba\u5b9a\u3002[\u7531 RefSeq \u63d0\u4f9b\uff0c2016 \u5e74 1 \u6708]<\/p>\n

 <\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

\"IR48-138<\/p>\n

\u6297\u7e96\u9023\u86cb\u767d\u6297\u9ad4 WB \u5716\u50cf(IREAL, #IR48-138)<\/span><\/strong><\/span>
\n1\/1000 \u7a00\u91cb\u7684\u6297\u7e96\u9023\u86cb\u767d\u6297\u9ad4\u3002\u6bcf\u6cf3\u9053 50 \u03bcg \u7684\u88c2\u89e3\u7269\u3002\u8a72\u5370\u8de1\u662f\u4f7f\u7528 5% SDS-PAGE \u751f\u6210\u7684\u3002\u785d\u9178\u7e96\u7dad\u7d20\u819c\u8207 IR48-138 \u5728 4\u00b0C \u4e0b\u5b75\u80b2\u904e\u591c\u3002<\/p>\n

\"IR48-138<\/p>\n

\u6297\u7e96\u9023\u86cb\u767d\u6297\u9ad4_\u4eba\u809d\u7d30\u80de\u764c (HCC) IHC \u5716\u50cf(IREAL, #IR48-138)<\/span><\/strong><\/span>
\n\u77f3\u881f\u5305\u57cb\u7684\u4eba\u809d\u7d30\u80de\u764c (HCC) \u7684\u514d\u75ab\u7d44\u7e54\u5316\u5b78\u5206\u6790\uff0c\u7528 1\/100 \u7684 IR48-138 \u6a19\u8a18\u7e96\u9023\u86cb\u767d\u3002<\/p>\n

\"IR48-138<\/p>\n

\u6297\u7e96\u9023\u86cb\u767d\u6297\u9ad4_\u4eba\u809d\u7d30\u80de\u764c (HCC) IHC \u5716\u50cf(iREAL, #IR48-138<\/span>)<\/span><\/strong>
\n\u77f3\u881f\u5305\u57cb\u7684\u4eba\u809d\u7d30\u80de\u764c (HCC) \u7684\u514d\u75ab\u7d44\u7e54\u5316\u5b78\u5206\u6790\uff0c\u7528 1\/100 \u7684 IR48-138 \u6a19\u8a18\u7e96\u9023\u86cb\u767d\u3002<\/p>\n

\"IR48-138<\/p>\n

\u6297\u7e96\u9023\u86cb\u767d\u6297\u9ad4 IHC \u5716\u50cf(IREAL, #IR48-138)<\/span><\/strong><\/span>
\n\u77f3\u881f\u5305\u57cb\u7684\u4eba\u764c\u7d44\u7e54\u7528 1\/100 \u7684 IR48-138 \u6a19\u8a18\u7e96\u9023\u86cb\u767d\u7684\u514d\u75ab\u7d44\u7e54\u5316\u5b78\u5206\u6790\u3002<\/p>\n

\"IR48-138<\/p>\n

\u6297\u7e96\u9023\u86cb\u767d\u6297\u9ad4 WB \u5716\u50cf(IREAL, #IR48-138)<\/span><\/strong><\/span>
\n\u6240\u6709\u6cf3\u9053\uff1a1\/500 \u7a00\u91cb\u7684\u6297\u7e96\u9023\u86cb\u767d\u6297\u9ad4\u3002\u6bcf\u6cf3\u9053 60 \u03bcg \u7684\u88c2\u89e3\u7269\u3002\u8a72\u5370\u8de1\u662f\u4f7f\u7528 5% SDS-PAGE \u751f\u6210\u7684\u3002\u7136\u5f8c\u7528 3%BSA \u5c01\u9589\u785d\u9178\u7e96\u7dad\u7d20\u819c 1 \u5c0f\u6642\uff0c\u7136\u5f8c\u5728 4\u00b0C \u4e0b\u8207 IR48-138 \u5b75\u80b2\u904e\u591c\u3002<\/p>\n

\"IR48-138<\/p>\n

\u6297\u7e96\u9023\u86cb\u767d\u6297\u9ad4 ICC\/IF \u5716\u50cf(IREAL, #IR48-138)<\/span><\/strong><\/span>
\n\u514d\u75ab\u7192\u5149\uff1a\u7d30\u80de\u5728\u5ba4\u6eab\u4e0b\u7528 4% \u591a\u805a\u7532\u919b\u56fa\u5b9a 10 \u5206\u9418\uff0c\u5728\u5ba4\u6eab\u4e0b\u7528 0.1% NP-40 \u900f\u5316 10 \u5206\u9418\uff0c\u7136\u5f8c\u5728\u5ba4\u6eab\u4e0b\u7528 5% BSA \u5c01\u9589 30 \u5206\u9418\u3002\u7d30\u80de\u5728 1:200 \u548c 4\u00b0C \u4e0b\u7528 IR48-138 \u6297\u7e96\u9023\u86cb\u767d\u6297\u9ad4\uff08\u7d05\u8272\uff09\u67d3\u8272\u3002DAPI\uff08\u85cd\u8272\uff09\u7528\u4f5c\u6838\u8907\u67d3\u5291\u3002<\/p>\n

\"IR48-138\u6297\u7e96\u9023\u86cb\u767d\u6297\u9ad4_\u4eba\u4e73\u817a\u764cIHC\u5716\u50cf\"<\/p>\n

\u6297\u7e96\u9023\u86cb\u767d\u6297\u9ad4_\u4eba\u4e73\u817a\u764cIHC\u5716\u50cf(IREAL, #IR48-138)<\/span><\/strong><\/span>
\n\u77f3\u881f\u5305\u57cb\u7684\u4eba\u4e73\u817a\u764c\u7528 1\/100 \u7684 IR48-138 \u6a19\u8a18\u7e96\u9023\u86cb\u767d\u7684\u514d\u75ab\u7d44\u7e54\u5316\u5b78\u5206\u6790\u3002<\/p>\n

\"IR48-138\u6297\u7e96\u9023\u86cb\u767d\u6297\u9ad4_\u4eba\u4e73\u817a\u764cIHC\u5716\u50cf\"<\/p>\n

\u6297\u7e96\u9023\u86cb\u767d\u6297\u9ad4_\u4eba\u4e73\u817a\u764cIHC\u5716\u50cf(IREAL, #IR48-138)<\/span><\/strong><\/span>
\n\u77f3\u881f\u5305\u57cb\u7684\u4eba\u4e73\u817a\u764c\u7528 1\/100 \u7684 IR48-138 \u6a19\u8a18\u7e96\u9023\u86cb\u767d\u7684\u514d\u75ab\u7d44\u7e54\u5316\u5b78\u5206\u6790\u3002
\n\"\"<\/p>\n

IHC\/\u75c5\u7406\u5b78\u6297\u9ad4(IHC\/Pathology antibodies)<\/span><\/strong><\/span><\/p>\n

Please note that related Positive Control Slides are also available. \u5206\u5b50\u75c5\u7406\u5b78(molecular pathology)\u4e2d\u7684\u514d\u75ab\u7d44\u7e54\u5316\u5b78(Immunohistochemistry)\u626e\u6f14\u91cd\u8981\u4f5c\u7528\uff1a\u8a3a\u65b7(Diagnosis)\u3001\u9810\u5f8c(Prognosis)\u548c\u9810\u6e2c(Predictive)\u3002 IHC \u6297\u9ad4(IHC antibodies)\u5df2\u88ab\u78ba\u5b9a\u70ba\u7528\u65bc\u798f\u723e\u99ac\u6797\u56fa\u5b9a\u77f3\u881f\u5207\u7247(formalin-fixed paraffin sections)\u514d\u75ab\u7d44\u7e54\u5316\u5b78(immunohistochemistry)\u7684\u6700\u4f73\u8a66\u5291\u3002\u6240\u6709 IHC\/\u75c5\u7406\u5b78\u6297\u9ad4(IHC\/Pathology antibodies)\u50c5\u4f9b\u7814\u7a76\u4f7f\u7528\u3002IHC\/\u75c5\u7406\u5b78\u6297\u9ad4\u5df2\u7d93\u91dd\u5c0d\u4eba\u9ad4\/\u8001\u9f20\/\u5927\u9f20\u7d44\u7e54\u7684\u514d\u75ab\u7d44\u7e54\u5316\u5b78\u9032\u884c\u4e86\u5ee3\u6cdb\u7684\u6e2c\u8a66\u548c\u9a57\u8b49(Pathology validated)\uff0c\u4e26\u7d93\u904e\u7cbe\u5fc3\u8a2d\u8a08\uff0c\u53ef\u63d0\u4f9b\u4e00\u81f4\u3001\u7279\u7570\u6027\u548c\u654f\u611f\u7684\u67d3\u8272\u3002<\/p>\n

PD-1\u75c5\u7406\u5b78\u6297\u9ad4\uff1a<\/span>\u7a0b\u5e8f\u6027\u6b7b\u4ea1 1 (Programmed Death 1, PD-1) \u662f T \u7d30\u80de\u8abf\u7bc0\u5291(T-cell regulators) CD28\/CTLA-4 \u5bb6\u65cf\u7684\u6210\u54e1\uff0c\u5728\u6d3b\u5316\u7684 T \u7d30\u80de\u3001B \u7d30\u80de\u548c\u5de8\u566c\u7d30\u80de\u8868\u9762\u8868\u9054\u70ba\u5171\u53d7\u9ad4(co-receptor)\u3002\u65b0\u7684\u7814\u7a76\u8868\u660e\uff0cPD-1\/PD-L1 \u4fe1\u865f\u901a\u8def\u53ef\u80fd\u8207\u6297\u816b\u7624\u514d\u75ab\u6709\u95dc\uff0c\u56e0\u70ba PD-L1 \u5df2\u88ab\u8b49\u660e\u53ef\u8a98\u5c0e\u6d3b\u5316 T \u7d30\u80de\u51cb\u4ea1(apoptosis)\u6216\u6291\u5236\u7d30\u80de\u6bd2\u6027 T \u7d30\u80de(cytotoxic T cells)\u7684\u6d3b\u6027\u3002\u8207 CD10 \u548c BCL6 \u4e0d\u540c\uff0cPD-1 \u50c5\u7531\u5c11\u6578 B \u7d30\u80de\u8868\u9054\uff0c\u56e0\u6b64\u6297 PD-1 \u53ef\u80fd\u662f\u8840\u7ba1\u514d\u75ab\u6bcd\u7d30\u80de\u6027\u6dcb\u5df4\u7624(angioimmunoblastic lymphoma)\u4e2d\u66f4\u7279\u7570\u548c\u6709\u7528\u7684\u8a3a\u65b7\u6a19\u8a8c\u7269\u3002>>\u00a0PD-L1\/CD274 C\u672b\u7aef\u6297\u9ad4<\/a><\/span><\/p>\n

CTLA4\u75c5\u7406\u5b78\u6297\u9ad4\uff1a<\/span>CD152 \u6297\u539f\uff0c\u4e5f\u7a31\u70ba CTLA4\uff0c\u662f\u4e00\u7a2e\u5c6c\u65bc\u514d\u75ab\u7403\u86cb\u767d\u8d85\u5bb6\u65cf(immunoglobulin superfamily)\u7684\u5b8c\u6574\u819c\u86cb\u767d\uff0c\u5176\u8868\u9054\u70ba 30 kDa \u55ae\u9ad4(30 kDa monomer)\u6216\u4e8c\u786b\u9375\u9023\u63a5\u7684\u540c\u578b\u4e8c\u805a\u9ad4(disulfide-linked homodimer)\u3002\u5b83\u8207T\u7d30\u80de\u6297\u539fCD28(T cell antigen CD28)\u5177\u6709\u540c\u6e90\u6027\uff0c\u8207CD28\u4e00\u6a23\uff0c\u662fCD80\u548cCD86\u7684\u914d\u9ad4(ligand)\u3002 CTLA-4 \u662f T \u8f14\u52a9\u7d30\u80de\u4e0a\u7684\u4e00\u7a2e\u53d7\u9ad4\uff0c\u53ef\u4f5c\u70ba\u514d\u75ab\u6aa2\u67e5\u9ede(immune checkpoint)\u548c\u514d\u75ab\u53cd\u61c9\u7684\u4e0b\u8abf\u56e0\u5b50\u3002 CTLA-4 \u7684\u7a81\u8b8a\u8207\u8a31\u591a\u81ea\u8eab\u514d\u75ab\u6027\u75be\u75c5(autoimmune diseases)\u6709\u95dc\u3002<\/p>\n

Lp-PLA2\u75c5\u7406\u5b78\u6297\u9ad4\uff1a<\/span>Lp-PLA2\uff0c\u4e5f\u7a31\u70ba\u8102\u86cb\u767d\u76f8\u95dc\u78f7\u8102\u9176 A2 (Lipoprotein-associated Phospholipase A2)\u6216\u8840\u5c0f\u677f\u6d3b\u5316\u56e0\u5b50\u4e59\u9170\u6c34\u89e3\u9176 (platelet-activating factor acetylhydrolase , PAF-AH)\uff0c\u662f\u4e00\u7a2e\u7531\u708e\u75c7\u7d30\u80de(inflammatory cells )\u7522\u751f\u7684\u9176\uff0c\u5176\u529f\u80fd\u662f\u4f7f\u8840\u5c0f\u677f\u6d3b\u5316\u56e0\u5b50(platelet-activating factor)\u5931\u6d3b\u548c\u964d\u89e3(degrade)\uff0c\u8840\u5c0f\u677f\u6d3b\u5316\u56e0\u5b50\u662f\u4e00\u7a2e\u6709\u6548\u7684\u4fc3\u708e\u6027\u78f7\u8102(pro-inflammatory phospholipid)\uff0c\u540c\u6642\u9084\u8ca0\u8cac\u6c34\u89e3\u6c27\u5316\u4fee\u98fe(hydrolyzing oxidatively modified)\u7684\u591a\u4e0d\u98fd\u548c\u8102\u80aa\u9178(polyunsaturated fatty acids)\u3002 Lp-PLA2 \u7684\u9176\u4fc3\u4f5c\u7528\u7522\u7269\u8207\u52d5\u8108\u7ca5\u6a23\u786c\u5316(atherosclerosis)\u7684\u767c\u5c55\u6709\u95dc\uff0c\u4e26\u8207\u51a0\u5fc3\u75c5(coronary heart disease)\u548c\u4e2d\u98a8(stroke)\u7684\u98a8\u96aa\u589e\u52a0\u5448\u6b63\u76f8\u95dc\u3002<\/p>\n

Muc2\u75c5\u7406\u5b78\u6297\u9ad4\uff1a<\/span>\u7c98\u86cb\u767d 2(Mucin 2)\uff0c\u4e5f\u7a31\u70ba MUC2\uff0c\u662f\u4e00\u7a2e\u7de8\u78bc\u7c98\u86cb\u767d\u5bb6\u65cf(mucin protein family)\u6210\u54e1\u7684\u4eba\u985e\u57fa\u56e0\u3002\u7531\u8a72\u57fa\u56e0\u7de8\u78bc\u7684\u86cb\u767d\u8cea\u5f62\u6210\u4fdd\u8b77\u8178\u8154(gut lumen)\u7684\u4e0d\u6eb6\u6027\u7c98\u6db2\u5c4f\u969c\u3002\u5728\u6b63\u5e38\u7d50\u8178(normal colon)\u3001\u4e73\u817a(breast)\u3001\u524d\u5217\u817a(prostate)\u548c\u553e\u6db2\u817a(salivary gland)\u7b49\u4eba\u9ad4\u7d44\u7e54\u4ee5\u53ca\u80c3\u8178\u9053(gastrointestinal)\u3001\u7d50\u8178(colonic)\u3001\u4e73\u817a(breast)\u548c\u524d\u5217\u817a\u816b\u7624(prostate neoplasia)\u4e2d\u6aa2\u6e2c\u5230 MUC2 \u8868\u9054\u3002<\/p>\n

\u7d30\u80de\u89d2\u86cb\u767d 18 (Cytokeratin 18) \u75c5\u7406\u5b78\u6297\u9ad4\uff1a<\/span>\u7d30\u80de\u89d2\u86cb\u767d 18 (Cytokeratin 18, CK 18) \u662f\u4e00\u7a2e 45kDa \u7684\u86cb\u767d\u8cea\uff0c\u5c6c\u65bc\u9178\u6027\u985e\u578b\u7684\u7d30\u80de\u89d2\u86cb\u767d(cytokeratins)\uff0c\u901a\u5e38\u5728\u7c21\u55ae\u7684\u975e\u5206\u5c64\u4e0a\u76ae\u7d30\u80de(non-stratified epithelia)\u4e2d\u8868\u9054\u3002\u7136\u800c\uff0cCK 18 \u4e5f\u5728\u79fb\u884c\u4e0a\u76ae(transitional epithelium)\u7684\u57fa\u5e95\u7d30\u80de\u548c\u8868\u5c64\u7d30\u80de\u4ee5\u53ca\u8907\u96dc\u4e0a\u76ae(complex epithelia)\u7684\u7ba1\u8154\/\u5206\u6ccc\u7d30\u80de(luminal\/secretory cells)\u4e2d\u8868\u9054\u3002 CK 18 \u6297\u9ad4\u5728\u8d77\u6e90\u65bc\u7c21\u55ae\u548c\u817a\u9ad4\u4e0a\u76ae(glandular epithelium)\u7684\u817a\u764c(Adenocarcinomas)\u4ee5\u53ca\u9c57\u72c0\u7d30\u80de\u764c(Squamous Carcinoma)\u7684\u4f4e\u5206\u5316\u816b\u7624\u7d30\u80de\u4e2d\u5448\u967d\u6027\u67d3\u8272\u3002<\/p>\n

MLH1\u75c5\u7406\u5b78\u6297\u9ad4\u00a0<\/span>\u662f\u4e00\u7a2e\u932f\u914d\u4fee\u5fa9\u86cb\u767d(mismatch repair protein)\uff0c\u8207 MSH2\u3001MSH6 \u548c PMS2 \u4e00\u8d77\u53c3\u8207\u7dad\u6301\u907a\u50b3\u4fe1\u606f\u7684\u5b8c\u6574\u6027\u3002\u9019\u7a2e\u86cb\u767d\u8cea\u901a\u5e38\u8207\u907a\u50b3\u6027(hereditary)\u975e\u606f\u8089\u75c5\u6027\u7d50\u76f4\u8178\u764c(non-polyposis colorectal cancer)\u6709\u95dc\uff0c\u56e0\u70ba MLH1 \u57fa\u56e0\u5728\u60a3\u6709\u9019\u7a2e\u764c\u75c7\u7684\u60a3\u8005\u4e2d\u7d93\u5e38\u767c\u751f\u7a81\u8b8a\u3002\u7814\u7a76\u8868\u660e\uff0cMLH1 \u5728\u5927\u90e8\u5206\u5fae\u885b\u661f\u4e0d\u7a69\u5b9a\u6027(microsatellite instability)\u4ee5\u53ca\u5b50\u5bae\u5167\u819c\u764c(endometrial)\u548c\u5375\u5de2\u764c(ovarian cancers)\u60a3\u8005\u4e2d\u5b58\u5728\u7f3a\u9677\u3002\u7576\u5728 IHC \u9762\u677f\u4e2d\u8207 MSH6\u3001MSH2 \u548c PMS2 \u914d\u5c0d\u6642\uff0c\u6297 MLH1 \u7684\u4f7f\u7528\u5f97\u5230\u512a\u5316\u3002<\/p>\n

HER2\u75c5\u7406\u5b78\u6297\u9ad4:<\/span><\/p>\n

HER2\u5728 10-20% \u7684\u4e73\u817a\u764c\u60a3\u8005(breast cancer patients)\u4e2d\u88ab\u8b58\u5225\u51fa\u4f86\u3002 Her2\/Neu (c-erbB-2) \u539f\u764c\u57fa\u56e0(proto-oncogene)\u662f\u4e00\u7a2e\u8de8\u819c\u53d7\u9ad4\u916a\u6c28\u9178\u6fc0\u9176(transmembrane receptor tyrosine kinase)\uff0c\u5728\u8a31\u591a\u764c\u75c7\u4e2d\u90fd\u6709\u81e8\u5e8a\u8868\u73fe\u3002 c-erbB-2 \u86cb\u767d\u7684\u904e\u5ea6\u8868\u9054(Overexpression)\u8207\u5c0e\u7ba1\u4e73\u817a\u764c(ductal breast cancer)\u4ee5\u53ca\u80ba\u817a\u764c(pulmonary)\u548c\u80c3\u817a\u764c(gastric adenocarcinomas)\u6709\u95dc\u3002Her2 \u548c p53 \u4e4b\u9593\u7684\u76f8\u95dc\u6027\u4e5f\u5df2\u88ab\u8b49\u660e\uff0c\u56e0\u70ba\u9019\u5169\u7a2e\u86cb\u767d\u7684\u904e\u5ea6\u8868\u9054\u8207\u8180\u80f1\u764c\u65e9\u671f\u4fb5\u8972(early invasion)\u548c\u8f49\u79fb(metastasis)\u6709\u95dc\u3002<\/p>\n

Ki-67\u00a0<\/span>\u662f\u4e00\u7a2e\u6838\u975e\u7d44\u86cb\u767d(nuclear non-histone protein)\uff0c\u5728\u975c\u6b62\u7d30\u80de(quiescent cells)\u4e2d\u4ee5\u4f4e\u6c34\u5e73\u5b58\u5728\uff0c\u4f46\u5728\u589e\u6b96\u7d30\u80de(proliferating cells)\u4e2d\u589e\u52a0\uff0c\u5c24\u5176\u662f\u5728 G2\u3001M \u548c S \u671f\u5f8c\u534a\u671f\u3002\u56e0\u6b64\uff0cKi-67 \u53cd\u61c9\u6027\uff08\u5b9a\u7fa9\u70ba\u901a\u904e IHC \u67d3\u8272\u6e2c\u91cf\u7684\u67d3\u8272\u967d\u6027\u7684\u816b\u7624\u7d30\u80de\u767e\u5206\u6bd4\uff09\u662f\u7d30\u80de\u589e\u6b96(cell proliferation)\u7684\u7279\u7570\u6027\u6838\u6a19\u8a18(nuclear marker )\u3002\u904e\u5ea6\u8868\u9054\u7d93\u5e38\u51fa\u73fe\u5728\u5404\u7a2e\u60e1\u6027\u7d44\u7e54(malignant tissues )\u4e2d\uff0c\u4e26\u4e14\u8207\u60a3\u6709\u795e\u7d93\u5167\u5206\u6ccc\u816b\u7624(neuroendocrine tumor)\u7684\u500b\u9ad4\u7684\u8f03\u5dee\u5b58\u6d3b\u7387\u76f8\u95dc\u3002>>IHC\u75c5\u7406\u5b78\u6297\u9ad4-Ki-67\u00a0\u6297\u9ad4<\/a>\u00a0<\/span><\/strong><\/span><\/p>\n

\"Better<\/p>\n

Exosome \u7814\u7a76\u5de5\u5177\/\u764c\u75c7\u76f8\u95dcExosomal Protein Marker-IReal Biotechnology<\/span><\/strong><\/span><\/p>\n

\u8b58\u5225\u5916\u6ccc\u9ad4(exosomes)\u7684\u6700\u5e38\u898b\u8de8\u819c\u6a19\u8a18\u4f86\u81ea\u56db\u8de8\u819c\u86cb\u767d\u5bb6\u65cf\uff08CD63 \u3001CD81 \u548c CD9\uff09\u3002\u8a72\u5bb6\u65cf\u4e2d\u7684\u86cb\u767d\u8cea\u5177\u6709\u56db\u500b\u8de8\u819c\u5340\u57df\uff0c\u4e26\u4e14\u5b9a\u4f4d\u65bc\u8cea\u819c\u4e2d\u5bcc\u542b\u56db\u8de8\u819c\u86cb\u767d\u7684\u5fae\u57df (\u00a0tetraspanin enriched microdomains, TEM)\u3002\uff08CD63 \u3001CD81 \u3001 CD9\u53caCD82\uff09\u8207\u7c98\u9644\u5206\u5b50(\u00a0adhesion molecules)\u3001\u8de8\u819c\u53d7\u9ad4(\u00a0transmembrane receptors)\u548c\u7d30\u80de\u5167\u4fe1\u865f\u86cb\u767d(intracellular signaling proteins)\u76f8\u95dc\uff0c\u4ee5\u4fc3\u9032\u548c\u8abf\u7bc0\u4fe1\u865f(\u00a0signaling events)\u4e8b\u4ef6\u3002\u9ad8\u8cea\u91cf\u5916\u6ccc\u9ad4(exosomes)\u55ae\u591a\u682a\u6297\u9ad4 (IREAL Antibody)\uff0c\u63d0\u4f9b\u60a8\u66f4\u591aExosome \u7814\u7a76\u5de5\u5177\uff0c\u53ef\u61c9\u7528\u65bcIHC\/IF\/WB\u6297\u9ad4\u3002IREAL\u63d0\u4f9b\u591a\u7a2e\u9ad8\u8cea\u91cf\u5916\u6ccc\u9ad4(exosomes)\u55ae\u591a\u682a\u6297\u9ad4 (IREAL Antibody)\uff0c\u5305\u62ecCD9(#IR300-981)<\/a>\u3001CD63(#IR301-983)<\/a>\u3001CD81(#IR302-986)<\/a>\u3001Flotillin-1(#IR304-990)<\/a>\u3001Alix(#IR303-988)<\/a>\u7b2c\u4e09\u65b9\u9a57\u8b49IHC\/IF\/WB\u6297\u9ad4\u3002\u5916\u6ccc\u9ad4(exosomes)\u53e6\u6709\u63d0\u4f9b\u5957\u7d44\u578b\u5f0f\u7684\u6297\u9ad4Panel\u5957\u7d44<\/a>\u3002<\/p>\n

 <\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716<\/p>\n

BRAND\/AGENCY\/<\/strong>\u53f0\u7063\u4ee3\u7406\u5546<\/strong><\/span>\/<\/span>\u00a0<\/a><\/span><\/strong>IREAL<\/strong>\u827e\u92b3\u53f0\u7063\u4ee3\u7406\u5546<\/strong><\/a><\/span><\/span><\/p>\n

IREAL\u827e\u92ed\u5b98\u7db2\u00a0https:\/\/www.irealbio.com\/<\/a><\/span><\/p>\n

IREAL\u827e\u92ed\u751f\u7269\u79d1\u6280\u63d0\u4f9b\u6642\u4e0b\u71b1\u9580\u7814\u7a76\u9818\u57dfEMT marker\u3001Apoptosis\u3001ER stress\u3001Cell Cycle\u3001Autophagy\u53caNeuroscience\u7b49\u5e38\u7528\u6297\u9ad4\uff0c\u4e26\u63d0\u4f9b\u5ba2\u88fd\u5316\u6297\u9ad4\u670d\u52d9\u5e73\u53f0\u3002iReal\u00a0\u827e\u92ed\u751f\u7269\u79d1\u6280\u63d0\u4f9b\u6642\u4e0b\u71b1\u9580\u7814\u7a76\u9818\u57dfEMT marker\u3001Apoptosis\u3001ER stress\u3001Cell Cycle\u3001Autophagy\u53caNeuroscience\u7b49\u5e38\u7528\u6297\u9ad4\uff0c\u4e26\u63d0\u4f9b\u5ba2\u88fd\u5316\u6297\u9ad4\u670d\u52d9\u5e73\u53f0\u3002iReal \u827e\u92b3\u751f\u6280\u7368\u5bb6\u6253\u9020\u51faMonoclonal-like Polyclonal Antibody\uff0c\u514d\u75ab\u539f(Immunogen)\u70ba\u61c9\u7528\u77ed\u93c8\u80dc\u80bd(peptide)\u70ba\u6297\u539f\u8a2d\u8a08\u51fa\u767c\u9ede\uff0c\u6311\u9078\u5404\u86cb\u767d\u6700\u4f73\u7684\u4f4d\u9ede(epitope) \u8fa8\u8b58\u4f4d\u4f86\u8fa8\u8a8d\u76ee\u6a19\u86cb\u767d\uff0ciReal\u00a0\u514d\u75ab\u8840\u6e05\u7d93\u904e\u4e00\u9023\u591a\u6b65\u9a5f\u7684\u7d14\u5316\u904e\u7a0b(Affinity column purified )\uff0c\u53bb\u9664\u6389\u5404\u7a2e\u975e\u5c08\u4e00\u6027\u7684\u86cb\u767d\uff0c\u4ee5\u964d\u4f4e\u6297\u9ad4\u7684\u80cc\u666f\u503c\uff1b\u540c\u6642\u85c9\u7531\u5154\u5b50\u7368\u7279\u7684\u514d\u75ab\u7cfb\u7d71\u6240\u7522\u751f\u7684\u6297\u9ad4\uff0c\u80fd\u5920\u7522\u2f63\u8a31\u591a\u2f7c\u2fcf\u514d\u75ab\u7cfb\u7d71\u4e0d\u80fd\u8b58\u5225\u7684\u6297\u539f\u7684\u6297\u9ad4\u3002\u64c1\u6709\u5404\u5b78\u8853\u55ae\u4f4d\u771f\u5be6\u6578\u64da\u7684\u9a57\u8b49\uff0c\u4e26\u5177\u5099\u81e8\u5e8a\u9a57\u8b49\u898f\u683c\u7684\u6297\u9ad4\u54c1\u8cea\uff0c\u5927\u91cf\u4e14\u73fe\u8ca8\u4f9b\u61c9IHC\/IF\/WB\u6297\u9ad4\u3002<\/p>\n

\"IREAL\u827e\u92b3\u514d\u75ab\u67d3\u8272\u6297\u9ad4-\u592a\u9f0e\u751f\u6280_\u5de5\u4f5c\u5340\u57df<\/p>\n

\"\u578b\u9304\u4e0b\u8f09\"<\/p>\n

\"\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

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