{"id":39423,"date":"2023-03-03T17:30:30","date_gmt":"2023-03-03T09:30:30","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=39423"},"modified":"2023-08-15T16:22:15","modified_gmt":"2023-08-15T08:22:15","slug":"%e9%90%a1iron%e5%91%88%e8%89%b2%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-iron-colorimetric-assay-kit%e8%b2%a8%e8%99%9f-abcam-ab83366-biovision-k390","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=39423","title":{"rendered":"\u9421Iron\u5448\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | Iron Colorimetric Assay Kit\u8ca8\u865f ABCAM ab83366 (BIOVISION K390)"},"content":{"rendered":"

Inorganic Ions\u7121\u6a5f\u96e2\u5b50\u6216\u96fb\u89e3\u8cea\u662f\u5c0d\u7d30\u80de\u751f\u7269\u7684\u6b63\u5e38\u529f\u80fd\u81f3\u95dc\u91cd\u8981\u7684\u7d44\u5206\uff0c\u4e26\u4e14\u6709\u52a9\u65bc\u652f\u6301\u8a31\u591a\u6d3b\u52d5\uff0c\u5305\u62ec\u9aa8\u9abc\/\u7259\u9f52\u7684\u5f62\u6210\uff0c\u795e\u7d93\u5143\u6d3b\u52d5\uff0c\u808c\u8089\u6536\u7e2e\u7b49\u3002BioVision\u64c1\u6709\u591a\u7a2e\u6e2c\u91cf\u65b9\u6cd5\uff0c\u7528\u65bc\u6e2c\u91cf\u591a\u7a2e\u7121\u6a5f\u96e2\u5b50\uff0c\u5305\u62ec\u9223\uff0c\u9435\uff0c\u9382\uff0c\u92c5\uff0c\u78f7\u9178\u9e7d\u7b49\u3002 \u8a72\u6e2c\u5b9a\u4f7f\u5f97a\u80fd\u5920\u5f9e\u5404\u7a2e\u6a23\u54c1\uff0c\u6e2c\u91cf\u9019\u4e9b\u5206\u5b50\u4e4b\u542b\u91cf\u3002\u6b64\u7522\u54c1\u9700\u7531\u5100\u5668\u9032\u884c\u5b9a\u91cf\u00a0<\/strong>ELISA<\/strong>\u5100\u5668\u8a2d\u5099\u8acb\u6309\u6b64<\/strong><\/a><\/span><\/p>\n

\u9421<\/strong>Iron<\/strong>\u5448\u8272<\/strong>\u6e2c\u5b9a\u8a66\u5291\u76d2 |\u00a0<\/strong>\u8ca8\u865fABCAM ab83366 (BIOVISION K390)<\/strong><\/a><\/span><\/p>\n

Iron Colorimetric Assay Kit<\/em><\/strong><\/p>\n

\u7522\u54c1\u4ecb\u7d39:<\/span>\u00a0<\/strong><\/span>\u9435\u5c0d\u5e7e\u4e4e\u6240\u6709\u5df2\u77e5\u7684\u751f\u7269\u4f86\u8aaa\u81f3\u95dc\u91cd\u8981\u3002 \u5b83\u901a\u5e38\u5b58\u5132\u5728\u91d1\u5c6c\u86cb\u767d\u8cea\u7684\u4e2d\u5fc3\uff0c\u5728\u8840\u7d05\u7d20\u8907\u5408\u7269\u548c\u6c27\u8f09\u9ad4\u86cb\u767d\u8cea\u4e2d\u3002 \u7121\u6a5f\u9435\u9084\u6709\u52a9\u65bc\u6c27\u5316\u9084\u539f\u53cd\u61c9\u5728\u8a31\u591a\u9176\u7684\u9435 – \u786b\u7c07\uff0c\u5982\u6c2e\u9176\u548c\u6c2b\u5316\u9176\u3002 BioVision\u7684\u9435\u6e2c\u5b9a\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u6e2c\u91cf\u6a23\u54c1\u4e2d\u4e9e\u9435\u548c\/\u6216\u9435\u96e2\u5b50\u7684\u7c21\u55ae\u65b9\u4fbf\u624b\u6bb5\u3002 \u5728\u6e2c\u5b9a\u4e2d\uff0c\u9435\u8f09\u9ad4\u86cb\u767d\u5c07\u5728\u9178\u7de9\u885d\u6db2\u5b58\u5728\u4e0b\u5c07\u9435\u96e2\u89e3\u6210\u6eb6\u6db2\u3002 \u9084\u539f\u6210\u9435\u5f62\u5f0f\uff08Fe 2+\uff09\u5f8c\uff0c\u9435\u8207Ferene S\u53cd\u61c9\u7522\u751f\u7a69\u5b9a\u7684\u8457\u8272\u7d61\u5408\u7269\uff0c\u4e26\u5728593nm\u8655\u7522\u751f\u5438\u5149\u5ea6\u3002 \u7de9\u885d\u6db2\u4e2d\u5305\u542b\u4e00\u7a2e\u7279\u6b8a\u7684\u87af\u5408\u5316\u5b78\u7269\u8cea\uff0c\u53ef\u4ee5\u963b\u64cb\u9285\u96e2\u5b50\uff08Cu2\u1da7\uff09\u7684\u5e72\u64fe\u3002 \u8a72\u8a66\u5291\u76d2\u6e2c\u91cf50\u03bcl\u6a23\u54c1\u4e2d\u7dda\u6027\u7bc4\u570d\u70ba0.4\u81f320 nmol\u7684\u9435\uff0c\u6216\u5404\u7a2e\u6a23\u54c1\u4e2d\u76848\u03bcM\u81f3400\u03bcM\u9435\u6fc3\u5ea6\u3002<\/p>\n

\"\"<\/p>\n

\u529f\u80fd\u7814\u7a76 – ab83366<\/strong><\/span><\/div>\n
\n

Leal SM Jr \u7b49\u4eba\u6aa2\u67e5\u4e86\u9435\u7684\u53ef\u7528\u6027\u662f\u5426\u8abf\u7bc0\u611f\u67d3\u4e2d\u7684\u771f\u83cc\u751f\u9577\uff0c\u56e0\u70ba\u771f\u83cc\u611f\u67d3\u6703\u5f15\u767c\u9435\u87af\u5408\u53cd\u61c9\u3002\u5728 48 \u5c0f\u6642\u5167\uff0c\u7d66\u4e88 Fe-\u8461\u805a\u91a3 (Fe-Dex) \u548c\u53bb\u9435\u80fa (Defox) \u7684\u5c0f\u9f20\u8207\u5a92\u4ecb\u7269\u8655\u7406\u7684\u5c0f\u9f20\u76f8\u6bd4\u986f\u793a\u51fa\u66f4\u9ad8\u7684\u771f\u83cc\u91cf (Fungal dsRed)\u3002\u4f7f\u7528\u9435\u6e2c\u5b9a\u8a66\u5291\u76d2 (ab83366) \u5b9a\u91cf\u5c0f\u9f20\u8840\u6e05\u4e2d\u7684\u9435\u542b\u91cf\u3002<\/p>\n

\"\"<\/p>\n

\u529f\u80fd\u7814\u7a76 – \u9435\u6e2c\u5b9a\u8a66\u5291\u76d2 (ab83366)<\/strong><\/span><\/div>\n
\n

\u4eba\u9ad4\u5c3f\u6db2\u4e2d\u6e2c\u91cf\u7684\u9435\u986f\u793a\u6fc3\u5ea6\uff08\u5fae\u6469\u723e\uff09<\/p>\n

\"\"<\/p>\n

\u529f\u80fd\u7814\u7a76 – \u9435\u6e2c\u5b9a\u8a66\u5291\u76d2 (ab83366)<\/strong><\/span><\/div>\n
\n

\u6e2c\u5b9a\u571f\u58e4\u6a23\u54c1\u4e2d\u7684\u53ef\u6eb6\u6027\u6e38\u96e2\u9435\uff085 \u03bcL 100 \u03bcL \u7de9\u885d\u6db2\uff0c\u5176\u4e2d\u652a\u62cc\u4e86 100 mg \u571f\u58e4\uff09\u30015 \u03bcL FBS \u548c 5 \u03bcL 100 \u03bcM \u9435\u6a19\u6e96\u6a23\u54c1\u3002<\/p>\n

\"\"<\/p>\n

\u529f\u80fd\u7814\u7a76 – \u9435\u6e2c\u5b9a\u8a66\u5291\u76d2 (ab83366)<\/strong><\/span><\/div>\n
\n

\u704c\u6ce8\u5927\u9f20\u809d\u52fb\u6f3f\u4e2d\u9435 (II) \u548c\u7e3d\u9435 (II+III) \u7684\u6e2c\u5b9a\uff08\u6bcf\u5b54\u4f7f\u7528\u76f8\u7576\u65bc 8 mg \u7684\u6fd5\u7d44\u7e54\uff09\u3002\u6578\u64da\u662f\u4f86\u81ea 2 \u500b\u7368\u7acb\u5fa9\u5236\u7684\u5e73\u5747\u503c\u00b1SEM\uff0c\u5728\u91cd\u8907\u5b54\u4e2d\u9032\u884c\u3002<\/p>\n

\"ELISA<\/a><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK390-100 (ABCAM ab83366)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (593 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThe kit can detect 8 \u00b5M to 400 \u00b5M iron concentration in various samples.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes ~ 3 hours
\n\u2022 Fast and convenient
\n\u2022 The assay is sensitive and stable
\n\u2022 The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Iron Assay Buffer
\n\u2022 Iron Probe
\n\u2022 Iron Reducer
\n\u2022 Iron Standard (100 mM)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5176\u5b83\u5546\u54c1\u00a0<\/strong>\u03b1-Galactosylceramide<\/strong><\/a>\u00a0|\u00a0\u03b1-Tocopheryl succinate<\/strong><\/a>\u00a0|\u00a0Wogonin<\/strong><\/a>\u00a0|\u00a0VK3-OCH3<\/strong><\/a>\u00a0|\u00a0Vinorelbine Tartrate<\/strong><\/a><\/p>\n

\u5f15\u7528\u6587\u737b<\/span><\/strong><\/span><\/p>\n

\n
Bai, T. et al., (2017) Haloperidol, a sigma receptor 1 antagonist, promotes ferroptosis in hepatocellular carcinoma cells,\u00a0Biochemical and Biophysical Research Communications, Sep.2017, 491(4):919-925<\/a><\/div>\n<\/div>\n
\n
Brown et al., Increased Ferroportin-1 Expression and Rapid Splenic Iron Loss Occur with Anemia Caused by\u00a0Salmonella enterica<\/em>\u00a0Serovar Typhimurium Infection in Mice.\u00a0Infect. Immun.,\u00a0Jun 2015; 83: 2290 – 2299.<\/a><\/div>\n<\/div>\n
\n
Aijun Qiao et al., MicroRNA-210 Decreases heme Levels by Targeting Ferrochelatase in Cardiomyocytes.\u00a0JAHA, Apr 2013; 2: e000121.<\/a><\/div>\n<\/div>\n
\n
D. E. Brown et al., Salmonella enterica Causes More Severe Inflammatory Disease in C57\/BL6 Nramp1G169 Mice Than Sv129S6 Mice.\u00a0Veterinary Pathology, Feb 2013; 0.1177\/0300985813478213.<\/a><\/div>\n<\/div>\n
\n
\n

Gurugirijha Rathnasamy et al., Iron and Iron Regulatory Proteins in Amoeboid Microglial Cells Are Linked to Oligodendrocyte Death in Hypoxic Neonatal Rat Periventricular White Matter through Production of Proinflammatory Cytokines and Reactive Oxygen\/Nitrogen Species,\u00a0J. Neurosci., Dec 2011; 31: 17982 – 17995.<\/a><\/p>\n

\n
Bai, T. et al., (2017) Haloperidol, a sigma receptor 1 antagonist, promotes ferroptosis in hepatocellular carcinoma cells,\u00a0Biochemical and Biophysical Research Communications, Sep.2017, 491(4):919-925<\/a><\/div>\n<\/div>\n
\n
Brown et al., Increased Ferroportin-1 Expression and Rapid Splenic Iron Loss Occur with Anemia Caused by\u00a0Salmonella enterica<\/em>\u00a0Serovar Typhimurium Infection in Mice.\u00a0Infect. Immun.,\u00a0Jun 2015; 83: 2290 – 2299.<\/a><\/div>\n<\/div>\n
\n
Aijun Qiao et al., MicroRNA-210 Decreases heme Levels by Targeting Ferrochelatase in Cardiomyocytes.\u00a0JAHA, Apr 2013; 2: e000121.<\/a><\/div>\n<\/div>\n
\n
D. E. Brown et al., Salmonella enterica Causes More Severe Inflammatory Disease in C57\/BL6 Nramp1G169 Mice Than Sv129S6 Mice.\u00a0Veterinary Pathology, Feb 2013; 0.1177\/0300985813478213.<\/a><\/div>\n<\/div>\n
\n
Gurugirijha Rathnasamy et al., Iron and Iron Regulatory Proteins in Amoeboid Microglial Cells Are Linked to Oligodendrocyte Death in Hypoxic Neonatal Rat Periventricular White Matter through Production of Proinflammatory Cytokines and Reactive Oxygen\/Nitrogen Species,\u00a0J. Neurosci., Dec 2011; 31: 17982 – 17995.<\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Inorganic Ions\u7121\u6a5f\u96e2\u5b50\u6216\u96fb\u89e3\u8cea\u662f\u5c0d\u7d30\u80de\u751f\u7269\u7684\u6b63\u5e38\u529f\u80fd\u81f3\u95dc\u91cd\u8981\u7684\u7d44\u5206\uff0c\u4e26\u4e14\u6709\u52a9\u65bc\u652f\u6301\u8a31\u591a\u6d3b\u52d5\uff0c\u5305\u62ec […]<\/p>\n","protected":false},"author":3,"featured_media":37625,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261],"tags":[5358],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/39423"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=39423"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/39423\/revisions"}],"predecessor-version":[{"id":39425,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/39423\/revisions\/39425"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/37625"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=39423"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=39423"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=39423"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}