{"id":39323,"date":"2023-03-02T17:13:25","date_gmt":"2023-03-02T09:13:25","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=39323"},"modified":"2026-05-20T16:24:50","modified_gmt":"2026-05-20T08:24:50","slug":"%e8%9e%a2%e5%85%89%e5%90%9e%e5%99%ac%e4%bd%9c%e7%94%a8%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-%e7%b4%85%e8%89%b2%e8%9e%a2%e5%85%89%ef%bd%9catt-bioquest-%e8%b2%a8%e8%99%9f21225-att-bioquest","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=39323","title":{"rendered":"\u87a2\u5149\u541e\u566c\u4f5c\u7528\u6aa2\u6e2c\u8a66\u5291\u76d2-\u7d05\u8272\u87a2\u5149\uff5cATT Bioquest \u8ca8\u865f21225 (ATT Bioquest)"},"content":{"rendered":"

\u87a2\u5149\u541e\u566c\u4f5c\u7528\u6aa2\u6e2c\u8a66\u5291\u76d2-\u7d05\u8272\u87a2\u5149<\/span>\uff5cATT Bioquest \u8ca8\u865f21225 (ATT Bioquest)<\/span><\/span><\/strong><\/a><\/p>\n

Cell Meter\u2122 Fluorimetric Phagocytosis Assay Kit *Red Fluorescence*<\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"<\/p>\n

\u541e\u566c\u4f5c\u7528(Phagocytosis)\u5b9a\u7fa9\u70ba\u7d30\u80de\u5c0d\u8cea\u819c\u5305\u819c(plasma-membrane envelope)\u5167\u9846\u7c92\u7684\u7d30\u80de\u651d\u53d6(cellular uptake)\u3002\u901a\u904e\u541e\u566c\u548c\u7834\u58de\u5165\u4fb5\u7684\u5fae\u751f\u7269\uff0c\u541e\u566c\u904e\u7a0b(phagocytosis)\u5728\u5148\u5929\u514d\u75ab\u53cd\u61c9(\u00a0innate immune response)\u4e2d\u81f3\u95dc\u91cd\u8981\u3002\u901a\u904e\u6e05\u9664\u51cb\u4ea1\u5c0f\u9ad4\u7dad\u6301\u7d44\u7e54\u7a69\u614b(tissue homeostasis)\u548c\u91cd\u5851(remodeling )\u4e5f\u9700\u8981\u541e\u566c\u4f5c\u7528\u3002\u541e\u566c\u4f5c\u7528(phagocytosis)\u7684\u651d\u53d6\u6a5f\u5236\u53d6\u6c7a\u65bc\u9846\u7c92\u7684\u5927\u5c0f\u3001\u53d7\u9ad4-\u914d\u9ad4\u76f8\u4e92\u4f5c\u7528(receptor-ligand)\u4ee5\u53ca\u7d30\u80de\u9aa8\u67b6(cytoskeleton)\u7684\u53c3\u8207\u3002\u4e00\u65e6\u5167\u5316(internalized)\uff0c\u541e\u566c\u9ad4\u8207\u6eb6\u9176\u9ad4(\u00a0lysosomes)\u878d\u5408\u5f62\u6210\u6b21\u7d1a\u541e\u566c\u6eb6\u9176\u9ad4(secondary phagolysosome f)\u9032\u884c\u6d88\u5316\uff0c\u5c0e\u81f4 pH \u503c\u9010\u6f38\u964d\u4f4e\u3002Cell Meter\u2122 \u87a2\u5149\u541e\u566c\u4f5c\u7528\u6aa2\u6e2c\u8a66\u5291\u76d2(Cell Meter\u2122 Fluorimetric Phagocytosis Assay Kit )\u63a1\u7528\u7368\u7279\u7684 pH \u4f9d\u8cf4\u6027 Protonex\u2122 600 \u7d05\u8272\u4e73\u81a0\u73e0\u7d50\u5408\u7269\u3002\u73e0\u5b50\u8655\u65bc\u96a8\u6642\u53ef\u7528\u7684\u61f8\u6d6e\u6db2\u4e2d\u3002\u8207\u5927\u591a\u6578\u73fe\u6709\u7684\u87a2\u5149\u67d3\u6599\u4e0d\u540c\uff0cProtonex\u2122 600 \u7d05\u8272-\u4e73\u81a0\u5fae\u73e0\u7d50\u5408\u7269\u5728\u7d30\u80de\u5916\u662f\u7121\u87a2\u5149\u7684\u3002\u7136\u800c\uff0c\u7576\u5b83\u5011\u5728\u9178\u6027\u541e\u566c\u9ad4(acidic phagosomes)\/\u541e\u566c\u6eb6\u9176\u9ad4(phagolysosomes)\u5167\u90e8\u6642\uff0c\u5b83\u7684\u87a2\u5149\u6703\u6025\u5287\u589e\u52a0\u3002\u9019\u4e00\u7279\u6027\u4f7f\u5176\u7121\u9700\u53f0\u76fc\u85cd\u6dec\u6ec5\u6b65\u9a5f(trypan blue quenching step )\u5373\u53ef\u8f15\u9b06\u4f7f\u7528\uff0c\u662f\u7814\u7a76\u541e\u566c\u4f5c\u7528\u53ca\u5176\u898f\u5f8b\u7684\u5f37\u5927\u5de5\u5177\u3002 Cell Meter\u2122 Fluorimetric Phagocytosis Assay Kit \u9084\u5305\u62ec\u4e00\u7a2e\u7da0\u8272\u87a2\u5149\u7d30\u80de\u6d3b\u529b\u67d3\u6599\uff0c\u53ef\u4ee5\u901a\u904e\u87a2\u5149\u986f\u5fae\u93e1\u540c\u6642\u6aa2\u6e2c\u6d3b\u7d30\u80de\u548c\u541e\u566c\u904e\u7a0b\u3002\u8a72\u6e2c\u5b9a\u6cd5\u4e5f\u9069\u7528\u65bc\u87a2\u5149\u9176\u6a19\u5100(\u00a0fluorescence micro-plate)\u548c\u6d41\u5f0f\u7d30\u80de\u8853\u6aa2\u6e2c(\u00a0flow cytometry detections)\u3002<\/p>\n

Component A: Protonex 600 Red-Latex Beads Conjugate1 vial (15 \u00b5L)<\/p>\n

Component B: CytoTrace\u2122 Green1 vial<\/p>\n

Component C: DMSO1 vial (100 \u00b5L)<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

\"\"<\/p>\n

Figure\u00a01.\u00a0<\/span>Examination of phagocytosis in RAW 264.7 cells using Cell Meter\u2122 Fluorimetric Phagocytosis Assay Kit (Cat# 21225). RAW 264.7 cells were incubated with (B) or without (A) Cytochalasin D for 30 min before Protonex\u2122 600 Red-Latex Beads in growth medium was added and incubated for 4 hours before Cell Tracker was added and incubated for 30 minutes. The images were taken using Keyence Fluorescence microscopy.<\/p>\n

\"\u00e5\u00bc\u0095\u00e7\u0094\u00a8\u00e6\u0096\u0087\u00e7\u008d\u00bb\"<\/p>\n

View all\u00a04 citations:\u00a0Citation Explorer<\/a><\/div>\n
Methylsulfonylmethane protects against lethal dose MRSA-induced sepsis through promoting M2 macrophage polarization<\/a>
\nAuthors:\u00a0Ma, Wei and Ao, Shengxiang and Zhou, Jianping and Li, Jiaxin and Liang, Xin and Yang, Xue and Zhang, Hao and Liu, Boyang and Tang, Wanqi and Liu, Haoru and others,
\nJournal:\u00a0Molecular Immunology\u00a0(2022):\u00a069–77<\/div>\n
The N 6-methyladenosine (m6A)-forming enzyme METTL3 facilitates M1 macrophage polarization through the methylation of STAT1 mRNA
\nAuthors:\u00a0Liu, Yihan and Liu, Zhujiang and Tang, Hao and Shen, Yicong and Gong, Ze and Xie, Nan and Zhang, Xu and Wang, Wengong and Kong, Wei and Zhou, Yuan and others,
\nJournal:\u00a0American Journal of Physiology-Cell Physiology\u00a0(2019):\u00a0C762–C775<\/div>\n
The N6-Methyladenosine (m6A)-Forming Enzyme METTL3 Facilitates M1 Macrophage Polarization through the Methylation of STAT1 mRNA<\/a>
\nAuthors:\u00a0Liu, Yihan and Liu, Zhujiang and Tang, Hao and Shen, Yicong and Gong, Ze and Xie, Nan and Zhang, Xu and Wang, Wengong and Kong, Wei and Zhou, Yuan and others, undefined
\nJournal:\u00a0American Journal of Physiology-Cell Physiology\u00a0(2019)<\/div>\n
PHD2 is a regulator for glycolytic reprogramming in macrophages<\/a>
\nAuthors:\u00a0Guentsch, Annemarie and Beneke, Angelika and Swain, Lija and Farhat, Katja and Nagarajan, Shunmugam and Wielockx, Ben and Raithatha, Kaamini and Dudek, Jan and Rehling, Peter and Zieseniss, Anke and others, undefined
\nJournal:\u00a0Molecular and Cellular Biology\u00a0(2016):\u00a0MCB–00236<\/div>\n
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\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716<\/div>\n
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https:\/\/www.aatbio.com\u00a0<\/a><\/span><\/strong><\/div>\n
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\u00a0AAT Bioquest \u4e00\u76f4\u81f4\u529b\u65bc\u5275\u65b0\u548c\u7a81\u7834\u6aa2\u6e2c\u6280\u8853\u7684\u754c\u9650\u3002\u5341\u591a\u5e74\u5f8c\uff0c\u9019\u4e00\u627f\u8afe\u4fdd\u6301\u4e0d\u8b8a\u3002\u6211\u5011\u7368\u7279\u7684\u3001\u7d9c\u5408\u6027\u7684\u5206\u6790\u958b\u767c\u65b9\u6cd5\u501f\u9452\u4e86\u6240\u6709\u7814\u7a76\u9818\u57df\uff0c\u5f9e\u5316\u5b78\u5230\u751f\u7269\u5b78\u518d\u5230\u4fe1\u606f\u5b78\u7b49\u7b49\uff0c\u5c0e\u81f4\u4e86\u4e00\u6ce2\u5229\u7528\u6bd4\u8272\u3001\u87a2\u5149\u548c\u767c\u5149\u6280\u8853\u7684\u65b0\u4e00\u4ee3\u5149\u5ea6\u63a2\u91dd\u3002\u8207\u6211\u5011\u5145\u6eff\u6fc0\u60c5\u7684\u79d1\u5b78\u5bb6\u548c\u5c08\u5bb6\u5718\u968a\u4e00\u8d77\uff0cAAT Bioquest \u81f4\u529b\u65bc\u89e3\u6c7a\u751f\u547d\u79d1\u5b78\u7814\u7a76\u3001\u8a3a\u65b7\u7814\u767c\u548c\u85e5\u7269\u767c\u73fe\u4e2d\u9762\u81e8\u7684\u4e00\u4e9b\u6700\u5177\u6311\u6230\u6027\u7684\u554f\u984c\u3002
\nAAT Bioquest\u5728\u5168\u7403\u64c1\u6709\u4e00\u652f\u7531\u7d93\u9a57\u8c50\u5bcc\u7684\u5c08\u696d\u5206\u92b7\u5546\u7d44\u6210\u7684\u5f37\u5927\u7db2\u7d61\uff0c\u70ba\u5f9e\u5c0f\u578b\u7814\u7a76\u6a5f\u69cb\u5230\u8ca1\u5bcc 500 \u5f37\u516c\u53f8\u7684\u5404\u7a2e\u5ba2\u6236\u7d44\u5408\u63d0\u4f9b\u5353\u8d8a\u7684\u7522\u54c1\u548c\u5b9a\u5236\u670d\u52d9\u3002AAT Bioquest \u00a05000 \u591a\u7a2e\u7522\u54c1\u76ee\u9304\u5305\u62ec Fluo-8\u00ae\u3001Cal-520\u00ae\u3001Cell Meter\u2122\u3001Amplite\u2122\u3001CytoTrace\u2122 \u548c CytoTell\u2122 \u7b49\u7522\u54c1\u7dda\uff0c\u9019\u4e9b\u54c1\u724c\u5728\u751f\u547d\u79d1\u5b78\u7814\u7a76\u754c\u7372\u5f97\u4e86\u8a8d\u53ef\u548c\u8b9a\u8b7d\u3002<\/div>\n
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<\/div>\n
\"AAT-Bioquest-e1661441736952\"<\/div>\n

Amine-Reactive Cell Meter\u2122 Fixable Viability Dyes<\/span><\/strong><\/span><\/h3>\n

Cell Meter\u2122 fixable viability dyes are membrane-impermeant, amine-reactive dyes designed to discriminate live and dead cell populations in flow cytometry. These dyes readily penetrate dead cells with compromised membrane integrity and bind irreversibly to cellular amine proteins and other intracellular components producing significant cellular fluorescence. In live cells, these dyes cannot penetrate the membrane leaving only cell-surface amines to bind with and dimly fluoresce. The difference in intensity between viable and dead cells is significant, making it easy to exclude dead cells from analysis. Because staining is robust and stable, cells labeled with Cell Meter\u2122 fixable viability dyes can be fixed, permeabilized, and probed for intracellular immunophenotyping. The Cell Meter\u2122 fixable viability dyes are available in 9 fluorescent stains to provide users greater flexibility in multicolor panel design. Dyes included in this series are excited by primary excitation sources, including the 405, 488, 633, and 647 nm laser lines, and emit in common channels.
\n\"\"
\n<\/span><\/p>\n

\n
Detection of Jurkat cell viability using Cell Meter\u2122 BX590 fixable viability dye. Jurkat cells were treated and stained with Cell Meter\u2122 BX590 (Cat No.\u00a022514<\/a>), fixed in 3.7% formaldehyde, and analyzed by flow cytometry. The dead cell population (blue peak) is easily distinguished from the live cell population (red peak) with the PE-Texas Red channel. Nearly identical results were obtained before and after fixation.<\/div>\n
<\/div>\n<\/div>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

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