{"id":39310,"date":"2023-03-02T14:29:40","date_gmt":"2023-03-02T06:29:40","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=39310"},"modified":"2023-04-19T11:01:58","modified_gmt":"2023-04-19T03:01:58","slug":"mtt%e7%b4%b0%e8%83%9e%e5%a2%9e%e6%ae%96%e5%88%86%e6%9e%90%e8%a9%a6%e5%8a%91%e7%9b%92-mtt-cell-proliferation-and-cytotoxicity-assay-kit%e8%b2%a8%e8%99%9fabx090676","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=39310","title":{"rendered":"MTT\u7d30\u80de\u589e\u6b96\u5206\u6790\u8a66\u5291\u76d2 | MTT Cell Proliferation and Cytotoxicity Assay Kit\u8ca8\u865fabx090676"},"content":{"rendered":"<p><span style=\"color: #33cccc;\"><a style=\"color: #33cccc;\" href=\"http:\/\/biopioneer.com.tw\/?p=39310\">MTT\u7d30\u80de\u589e\u6b96\u5206\u6790\u8a66\u5291\u76d2\u00a0<strong>|\u00a0MTT Cell Proliferation and Cytotoxicity Assay Kit<\/strong><strong>\u8ca8\u865fabx090676<\/strong>\u00a0\u00a0<\/a><\/span><\/p>\n<p><span style=\"color: #999999;\">MTT Cell Proliferation and Cytotoxicity Assay Kit<\/span><br \/>\nMTT\u662f\u4e00\u7a2e\u6aa2\u6e2c\u7d30\u80de\u589e\u6b96(cell proliferation)\u548c\u7d30\u80de\u6bd2\u6027(cytotoxicity)\u7684\u8a66\u5291\u76d2\u3002 MTT\u00a0[3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide] [3-(4,5-\u4e8c\u7532\u57fa-2-\u567b\u5511\u57fa)-2,5-\u4e8c\u82ef\u57fa-2-H-\u56db\u5511\u6eb4\u5316\u7269]\uff0c\u4e00\u7a2e\u9ec3\u8272\u56db\u5511(yellow tetrazole)\uff0c\u88ab\u7c92\u7dda\u9ad4\u812b\u6c2b\u9176(mitochondrial dehydrogenases)\u9084\u539f\u70ba\u4e0d\u6eb6\u6027\u7d2b\u8272\u7d50\u6676\u7532\u81e2\u3002\u00a0\u5177\u6709\u6d3b\u6027\u4ee3\u8b1d\u7684\u6d3b\u7d30\u80de\u5c07MTT\u8f49\u5316\u70ba\u00a0formazan\uff0c\u7136\u800c\uff0c\u6b7b\u7d30\u80de\u55aa\u5931\u4e86\u9019\u7a2e\u80fd\u529b\u3002 \u56e0\u6b64\uff0c\u984f\u8272\u5f62\u6210\u50c5\u4f5c\u70ba\u6d3b\u7d30\u80de\u7684\u6709\u7528\u4e14\u65b9\u4fbf\u7684\u6a19\u8a18\u3002 \u6e2c\u5f97\u7684\u5438\u5149\u5ea6\uff08570nm\uff09\u8207\u6d3b\u7d30\u80de\u6578\u6210\u6bd4\u4f8b\u3002\u9ad8 O.D.s \u8868\u793a\u7d30\u80de\u589e\u6b96\uff0c\u800c\u4f4e O.D.s \u8868\u793a\u7d30\u80de\u6bd2\u6027\u3002\u4f7f\u7528\u63d0\u4f9b\u7684 Formazan Diluent Buffer \u6642\uff0c\u7121\u9700\u53bb\u9664\u56fa\u6709\u7684\u7d30\u80de\u57f9\u990a\u57fa\uff0c\u56e0\u6b64\u53ef\u4ee5\u907f\u514d\u4e0d\u5fc5\u8981\u7684\u6e2c\u5b9a\u932f\u8aa4\u3002\u8a72\u6aa2\u6e2c\u8a66\u5291\u76d2\u70ba\u63d0\u4f9b\u4e86easy-to-use\uff0cnon-radioactive\u975e\u653e\u5c04\u6027\u548chigh-throughput method\u00a0\u9ad8\u901a\u91cf\u7684\u65b9\u6cd5\u3002\u8a55\u4f30\u7d30\u80de\u4e4b cell proliferation\u7d30\u80de\u589e\u6b96\u3001\u00a0cell viability\u00a0chemotaxis\u8da8\u5316\u6027\u3001cytotoxicity\u7d30\u80de\u6bd2\u6027\u548c\u00a0apoptosis\u7d30\u80de\u51cb\u4ea1\u3002<\/p>\n<ul>\n<li>MTT Staining Solution: 5 ml<\/li>\n<li>Formazan Diluent Buffer: 55 ml<\/li>\n<\/ul>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong><em>Non-radioactive and high-throughput method to measure cell proliferation\/viability\/cytotoxicity<\/em><\/strong><\/span><\/p>\n<ul>\n<li>Assay type: Quantitative<\/li>\n<li>Detection method: Colorimetric<\/li>\n<li>Platform: Microplate reader<\/li>\n<li>Assay time: 3 hr 15 min<\/li>\n<li>Sample type: Adherent cells, Suspension cells<\/li>\n<\/ul>\n<table class=\"table table-bordered table-responsive table-striped\">\n<tbody>\n<tr>\n<td>Target<\/td>\n<td>MTT Cell Proliferation and Cytotoxicity<\/td>\n<\/tr>\n<tr>\n<td>Form<\/td>\n<td>Liquid<\/td>\n<\/tr>\n<tr>\n<td>Storage<\/td>\n<td>Store the MTT Staining Solution at -20 \u00b0C in the dark for up to one year. Store the Formazan Diluent Buffer between 2-8\u00b0C for up to one year.<\/td>\n<\/tr>\n<tr>\n<td>Directions for use<\/td>\n<td><strong>Protocol<\/strong><\/p>\n<ol>\n<li>Bring all reagents to room temperature before use.<\/li>\n<li>Collect logarithmic phase cells and adjust the cell suspension concentration. Add 100 \u00b5l into each well of a cell-culture plate and adjust the cell density to 1000-10,000 cells per well (sterile PBS should be added to the edge of the wells of the plate).<\/li>\n<li>Seed cells in a 5% CO<sub>2<\/sub>\u00a0incubator in the dark at 37 \u00b0C until cells are uniform at the bottom of the wells. The cell number for each well should be determined by cells&#8217; size and proliferation rate. Add 0-10 \u00b5l of the compound of interest per well (for a concentration gradient, this can be added after cells adhere, or every 2 hours, or every half-day, etc). It is recommended to set 5 wells to analyse in duplicate.<\/li>\n<li>Incubate the cells for 16-48 hours, then observe under microscope by inverting the cells.<\/li>\n<li>Add 10 \u00b5l of MTT Staining Solution into each well. Culture for 4 hours. If the compound of interest can react with MTT, centrifuge and remove the cultured medium. Rinse with PBS carefully 2-3 times, then add the MTT-containing cultured medium.<\/li>\n<li>Add 100 \u00b5l of Formazan Diluent Buffer into each well, then mix gently at low speed for 10-30 min until the crystal is dissolved completely. If undissolved Formazan is still present, it is recommended to pipette up and down gently, avoiding bubbles, 2-3 times, or incubate overnight. Measure the O.D. value at 570 nm.<\/li>\n<li>Set blank wells (cell culture medium, MTT Staining Solution, Formazan diluent buffer) and control wells (cells, media containing the compound of interest with the same concentration, culture medium, MTT Staining Solution, Formazan diluent buffer).<\/li>\n<\/ol>\n<p><strong>Notes:<\/strong><\/p>\n<ul>\n<li>Ensure that the wells do not dry out due to evaporation, particularly when the cell culture time is long. Avoid using the outermost wells; use PBS, water or culture medium to replace any liquid that has evaporated; or place the 96-well plate near water in the incubator.<\/li>\n<li>The MTT Staining Solution should not be used if it has turned a gray-green colour.<\/li>\n<li>If a precipitate forms in the Formazan Diluent Buffer, heat using a 37 \u00b0C water bath until the precipitate dissolved.<\/li>\n<li>Incubation steps should be carried out in the dark.<\/li>\n<li>Wear Personal Protective Equipment (PPE), such as lab coats, lab glasses and gloves, when using this product.<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong><br \/>\n<img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/abbkine%E5%95%86%E5%93%81%E6%8F%92%E5%9C%96-300x207.jpg\" alt=\"abbkine\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\" \/><img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/abm%E5%95%86%E5%93%81%E6%8F%92%E5%9C%96-300x207.jpg\" alt=\"abm\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\" \/><img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/biovision%E5%95%86%E5%93%81%E6%8F%92%E5%9C%96-300x207.jpg\" alt=\"biovision\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\" \/><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>MTT\u7d30\u80de\u589e\u6b96\u5206\u6790\u8a66\u5291\u76d2\u00a0|\u00a0MTT Cell Proliferation and Cytotoxicity  [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":33277,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[760,5340],"tags":[5365],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/39310"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=39310"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/39310\/revisions"}],"predecessor-version":[{"id":39311,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/39310\/revisions\/39311"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/33277"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=39310"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=39310"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=39310"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}