{"id":38805,"date":"2023-02-23T10:14:07","date_gmt":"2023-02-23T02:14:07","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=38805"},"modified":"2023-02-27T10:38:28","modified_gmt":"2023-02-27T02:38:28","slug":"%e4%ba%ba-gnly%ef%bc%88%e9%a1%86%e7%b2%92%e6%ba%b6%e7%b4%a0%ef%bc%89elisa%e8%a9%a6%e5%8a%91%e7%9b%92-elk-biotechnology%e8%b2%a8%e8%99%9felk2059","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=38805","title":{"rendered":"\u4eba GNLY\uff08\u9846\u7c92\u6eb6\u7d20\uff09ELISA\u8a66\u5291\u76d2 -ELK Biotechnology\u8ca8\u865fELK2059"},"content":{"rendered":"

\u4eba GNLY\uff08\u9846\u7c92\u6eb6\u7d20\uff09 ELISA\u8a66\u5291\u76d2 -ELK Biotechnology\u8ca8\u865fELK2059<\/span><\/strong><\/a><\/span><\/p>\n

Human GNLY(Granulysin) ELISA Kit<\/p>\n

\u9748\u654f\u5ea60.24-15 ng\/mL\u3001\u6700\u4f4e\u9748\u654f\u5ea60.08 ng\/mL<\/span><\/p>\n

\u9846\u7c92\u6eb6\u7d20(Granulysin)\u662f\u4e00\u7a2e\u5b58\u5728\u65bc\u7d30\u80de\u6bd2\u6027 T \u7d30\u80de(cytotoxic T cells )\u548c\u81ea\u7136\u6bba\u50b7\u7d30\u80de(\u00a0natural killer cells)\u7684\u7d30\u80de\u6bd2\u6027\u9846\u7c92\u4e2d\u7684\u86cb\u767d\u8cea\u3002\u9846\u7c92\u6eb6\u7d20(\u00a0Granulysin)\u662f\u985e\u7682\u82f7\u86cb\u767d (SAPLIP) \u5bb6\u65cf\u7684\u6210\u54e1\uff0c\u5728\u6297\u539f\u523a\u6fc0\u5f8c\u5f9e\u7d30\u80de\u6bd2\u6027 T \u7d30\u80de\u4e2d\u91cb\u653e\u51fa\u4f86\u3002\u9846\u7c92\u6eb6\u7d20(\u00a0Granulysin)\u5c0d\u7d50\u6838\u5206\u679d\u687f\u83cc\u548c\u5176\u4ed6\u751f\u7269\u9ad4\u5177\u6709\u6297\u5fae\u751f\u7269\u6d3b\u6027\u3002\u9846\u7c92\u6eb6\u7d20(\u00a0Granulysin)\u88ab\u4ea4\u66ff\u526a\u63a5\uff0c\u7522\u751f NKG5 \u548c 519 \u8f49\u9304\u7269\u3002\u9846\u7c92\u6eb6\u7d20(\u00a0Granulysin)\u662f\u4e00\u7a2e\u7d30\u80de\u6eb6\u89e3\u548c\u4fc3\u708e\u5206\u5b50\uff0c\u9996\u5148\u901a\u904e\u7be9\u9078\u201c\u665a\u671f\u201d\uff083-5 \u5929\uff09\u8868\u9054\u7684\u57fa\u56e0\u4f86\u9451\u5b9a\u4eba\u5916\u5468\u8840\u55ae\u6838\u7d30\u80de\u7684\u6d3b\u5316\u3002\u9846\u7c92\u6eb6\u7d20(\u00a0Granulysin)\u5b58\u5728\u65bc\u7d30\u80de\u6bd2\u6027 T \u6dcb\u5df4\u7d30\u80de (CTL) \u548c\u81ea\u7136\u6bba\u50b7 (NK) \u7d30\u80de\u7684\u7d30\u80de\u6eb6\u89e3\u9846\u7c92\u4e2d\u3002\u9846\u7c92\u6eb6\u7d20(\u00a0Granulysin)\u88ab\u88fd\u6210 15 kD \u5206\u5b50\uff0c\u5b83\u7684\u4e00\u90e8\u5206\u5728\u6c28\u57fa\u548c\u7fa7\u57fa\u672b\u7aef\u88ab\u5207\u5272\u6210 9 kD \u5f62\u5f0f\u3002<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong><\/span><\/p>\n

\u4eba GNLY\uff08\u9846\u7c92\u6eb6\u7d20\uff09ELISA\u8a66\u5291\u76d2 | \u8ca8\u865fELK2059 (Sandwich ELISA)\uff0c\u5075\u6e2c\u7684\u9748\u654f\u5ea60.24-15 ng\/mL\u3001\u6700\u4f4e\u9748\u654f\u5ea60.08 ng\/mL\u3002ELISA\u539f\u7406\u70ba\u5229\u7528\u6297\u9ad4\u8207\u6297\u539f\u4e4b\u9593\u7684\u9ad8\u5ea6\u5c08\u4e00\u6027\uff0c\u505a\u70ba\u5b9a\u91cf\u6a19\u8a8c\u7684\u65b9\u6cd5\uff1b\u7121\u8ad6\u6e2c\u91cf\u6297\u539f\u6216\u6e2c\u91cf\u6297\u9ad4\u90fd\u6709\u5f88\u9ad8\u7684\u9748\u654f\u5ea6\u8ddf\u5c08\u4e00\u6027\u3002\u8a72\u6aa2\u6e2c\u65b9\u6cd5\u5c0d\u4eba GNLY\uff08\u9846\u7c92\u6eb6\u7d20\uff09\u5177\u6709\u9ad8\u9748\u654f\u5ea6\u548c\u51fa\u8272\u7684\u7279\u7570\u6027\u3002ELISA\u5546\u54c1\u7684\u578b\u5f0f\u591a\u5143\uff0c \u63d0\u4f9b\u4e09\u660e\u6cbbELISA\u3001\u7af6\u722d\u578bELISA\u3002\u53ef\u61c9\u7528\u65bcserum, plasma, tissue homogenates, cell lysates, cell culture\u3002\u4e26\u5728 450nm \u00b1 10nm \u7684\u6ce2\u9577\u4e0b\u7528\u5206\u5149\u5149\u5ea6\u8a08\u6e2c\u91cf\u984f\u8272\u8b8a\u5316\u3002\u7136\u5f8c\u901a\u904e\u5c07\u6a23\u54c1\u7684 OD \u8207\u6a19\u6e96\u66f2\u7dda\u9032\u884c\u6bd4\u8f03\u4f86\u78ba\u5b9a\u6a23\u54c1\u4e2d\u4eba GNLY\uff08\u9846\u7c92\u6eb6\u7d20\uff09 \u7684\u6fc3\u5ea6\u3002<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product name:<\/td>\nHuman GNLY(Granulysin) ELISA Kit<\/td>\n<\/tr>\n
Reactivity:<\/td>\nHuman<\/td>\n<\/tr>\n
Alternative Names:<\/td>\nNKG5; LAG-2; TLA519; T-Lymphocyte Activation Gene 519<\/td>\n<\/tr>\n
Assay Type:<\/td>\nSandwich<\/td>\n<\/tr>\n
Sensitivity:<\/td>\n0.08 ng\/mL<\/td>\n<\/tr>\n
Standard:<\/td>\n15 ng\/mL<\/td>\n<\/tr>\n
Range:<\/td>\n0.24-15 ng\/mL<\/td>\n<\/tr>\n
Sample Type:<\/td>\nserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids<\/td>\n<\/tr>\n
Assay Length:<\/td>\n3.5h<\/td>\n<\/tr>\n
Research Area:<\/td>\nTumor immunity;Infection immunity;Immune molecule;<\/td>\n<\/tr>\n
Test principle:<\/td>\nThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Granulysin(GNLY). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Granulysin(GNLY). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Granulysin(GNLY), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm \u00b1 10nm. The concentration of Granulysin(GNLY) in the samples is then determined by comparing the OD of the samples to the standard curve.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\"\"<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716<\/p>\n

ELK<\/strong>\u539f\u5ee0\u7db2\u5740<\/strong><\/span>\u00a0 >><\/span>\u00a0http:\/\/www.elkbiotech.com\/<\/a>\u00a0<\/span><\/strong><\/span><\/p>\n

ELK Biotechnology\u516c\u53f8\u662f\u4e00\u5bb6\u5c08\u6ce8\u65bc\u751f\u547d\u79d1\u5b78\u3001\u514d\u75ab\u7814\u7a76\u7684\u9ad8\u79d1\u6280\u751f\u7269\u4f01\u696d\uff0c\u4f4d\u65bcBiolake, Donghu New & High Technology Development Zone, Wuhan city.ELK Biotechnology\u662f\u4e00\u5bb6\u5c08\u6ce8\u65bc\u514d\u75ab\u7522\u54c1\u548c\u5206\u5b50\u751f\u7269\u5b78\u7522\u54c1\u7814\u767c\u548c\u751f\u7522\u7684\u9ad8\u79d1\u6280\u751f\u7269\u516c\u53f8\u3002\u7522\u54c1\u6db5\u84cbELISA\u8a66\u5291\u76d2\u3001\u6297\u9ad4\u3001\u57fa\u56e0\u7d44\u63d0\u53d6\u8a66\u5291\u3001\u5206\u5b50\u751f\u7269\u5b78\u8a66\u5291\u3002\u6d89\u53ca\u514d\u75ab\u5316\u5b78\u3001\u816b\u7624\u7814\u7a76\u3001\u795e\u7d93\u751f\u7269\u5b78\u3001\u7d30\u80de\u9031\u671f\u3001\u4fe1\u865f\u8f49\u5c0e\u7b49\u9818\u57df\u3002\u6211\u5011\u7684\u7522\u54c1\u6709\u56b4\u683c\u7684\u7814\u767c\u548c\u8cea\u91cf\u6aa2\u6e2c\u6d41\u7a0b\u3002\u6bcf\u500b\u7522\u54c1\u90fd\u6709\u5b8c\u6574\u7684\u7814\u767c\u3001\u751f\u7522\u548c\u8cea\u6aa2\u8a18\u9304\u3002\u7d93\u9a57\u8c50\u5bcc\u7684\u7814\u767c\u4eba\u54e1\uff0c\u5b8c\u5584\u7684\u8cea\u6aa2\u652f\u6301\u90e8\u9580\uff0c\u56b4\u683c\u7684\u6aa2\u6e2c\u6d41\u7a0b\uff0c\u70ba\u5ba2\u6236\u63d0\u4f9b\u8cea\u91cf\u53ef\u9760\u7684\u79d1\u7814\u7522\u54c1\u3002<\/p>\n

\u200b<\/span>\"ELK1.jpg\"\u200b<\/span><\/span>\u200b<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

\u4eba GNLY\uff08\u9846\u7c92\u6eb6\u7d20\uff09 ELISA\u8a66\u5291\u76d2 -ELK Biotechnology\u8ca8\u865fELK2059 Human […]<\/p>\n","protected":false},"author":3,"featured_media":34915,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[452,3],"tags":[5303],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/38805"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=38805"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/38805\/revisions"}],"predecessor-version":[{"id":38842,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/38805\/revisions\/38842"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/34915"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=38805"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=38805"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=38805"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}