{"id":38734,"date":"2023-02-22T10:35:24","date_gmt":"2023-02-22T02:35:24","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=38734"},"modified":"2023-02-27T10:38:55","modified_gmt":"2023-02-27T02:38:55","slug":"%e5%92%96%e5%95%a1%e5%9b%a0caffeine%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-creative-diagnostics%e8%b2%a8%e8%99%9fcatdeia6842","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=38734","title":{"rendered":"\u5496\u5561\u56e0(Caffeine)\u6aa2\u6e2c\u8a66\u5291\u76d2 | Creative Diagnostics\u8ca8\u865fCat#DEIA6842"},"content":{"rendered":"

\u5496\u5561\u56e0(Caffeine)\u6aa2\u6e2c\u8a66\u5291\u76d2 | Creative Diagnostics\u00a0\u8ca8\u865fCat. No DEIA6842<\/a><\/span><\/strong><\/p>\n

\"\u5496\u5561\u56e0(Caffeine)\u6aa2\u6e2c\u8a66\u5291\u76d2<\/p>\n

\u5496\u5561\u56e0(Caffeine)\u662f\u4e00\u7a2e\u82e6\u6f80\u7684\u767d\u8272\u7d50\u6676\u9ec3\u560c\u5464\u751f\u7269\u9e7c(\u00a0xanthine alkaloid)\uff0c\u53ef\u4f5c\u70ba\u8208\u596e\u5291\u85e5\u7269(stimulant drug)\u3002 \u5728\u4e00\u4e9b\u690d\u7269\u7684\u7a2e\u5b50(\u00a0seeds)\u3001\u8449\u5b50(leaves)\u548c\u679c\u5be6(fruit )\u4e2d\u767c\u73fe\u4e86\u4e0d\u540c\u6578\u91cf\u7684\u5496\u5561\u56e0\uff0c\u5b83\u4f5c\u70ba\u4e00\u7a2e\u5929\u7136\u6bba\u87f2\u5291\u53ef\u4ee5\u9ebb\u75fa\u548c\u6bba\u6b7b\u4ee5\u690d\u7269\u70ba\u98df\u7684\u67d0\u4e9b\u6606\u87f2\u3002 \u4eba\u5011\u6700\u5e38\u98df\u7528\u5f9e\u5496\u5561\u690d\u7269\u7a2e\u5b50\u548c\u8336\u6a39\u8449\u4e2d\u63d0\u53d6\u7684\u6d78\u6db2\uff0c\u4ee5\u53ca\u542b\u6709\u53ef\u6a02\u679c\u884d\u751f\u7522\u54c1\u7684\u5404\u7a2e\u98df\u54c1\u548c\u98f2\u6599\u3002 \u5176\u4ed6\u4f86\u6e90\u5305\u62ec\u99ac\u9edb\u8336(\u00a0yerba mat\u00e9)\u3001\u74dc\u62c9\u90a3\u6f3f\u679c(\u00a0guarana berries)\u3001\u756a\u77f3\u69b4(guayusa)\u548c yaupon \u51ac\u9752(yaupon holly)\u3002Caffeine is a bitter, white crystalline xanthine alkaloid that acts as a stimulant drug. Caffeine is found in varying quantities in the seeds, leaves, and fruit of some plants, where it acts as a natural pesticide that paralyzes and kills certain insects feeding on the plants. It is most commonly consumed by humans in infusions extracted from the seed of the coffee plant and the leaves of the tea bush, as well as from various foods and drinks containing products derived from the kola nut. Other sources include yerba mat\u00e9, guarana berries, guayusa, and the yaupon holly.<\/p>\n

\u8a72\u6e2c\u8a66\u662f\u76f4\u63a5\u7af6\u722d\u6027 ELISA(direct competitive ELISA)\uff0c\u5176\u57fa\u65bc\u7279\u5b9a\u6297\u9ad4(specific antibodies)\u5c0d\u5496\u5561\u56e0\u7684\u8b58\u5225\u3002 \u7576\u6a23\u54c1\u4e2d\u5b58\u5728\u5496\u5561\u56e0\u6642\uff0c\u5496\u5561\u56e0-HRP \u985e\u4f3c\u7269\u6703\u7af6\u722d\u6eb6\u6db2\u4e2d\u5c0f\u9f20\u6297\u5496\u5561\u56e0\u6297\u9ad4\u7684\u7d50\u5408\u4f4d\u9ede\u3002 \u7136\u5f8c\u5496\u5561\u56e0\u6297\u9ad4\u8207\u56fa\u5b9a\u5728\u5fae\u91cf\u6ef4\u5b9a\u677f\u5b54\u4e2d\u7684\u4e8c\u6297\uff08\u5c71\u7f8a\u6297\u5c0f\u9f20\uff09\u7d50\u5408\u3002 \u5728\u6d17\u6ecc\u6b65\u9a5f\u548c\u6dfb\u52a0\u5e95\u7269\u6eb6\u6db2\u5f8c\uff0c\u7522\u751f\u984f\u8272\u4fe1\u865f\u3002 \u85cd\u8272\u7684\u5f37\u5ea6\u8207\u6a23\u54c1\u4e2d\u5b58\u5728\u7684\u5496\u5561\u56e0\u6fc3\u5ea6\u6210\u53cd\u6bd4\u3002 \u7279\u5b9a\u6642\u9593\u5f8c\u505c\u6b62\u986f\u8272\u53cd\u61c9\uff0c\u4e26\u4f7f\u7528 ELISA \u95b1\u8b80\u5668\u8a55\u4f30\u984f\u8272\u3002 \u6a23\u54c1\u7684\u6fc3\u5ea6\u901a\u904e\u4f7f\u7528\u6bcf\u6b21\u904b\u884c\u69cb\u5efa\u7684\u6a19\u6e96\u66f2\u7dda\u9032\u884c\u63d2\u503c\u4f86\u78ba\u5b9a\u3002<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

1. Microtiter plate coated with a second antibody (goat anti-mouse)<\/p>\n

2. Standards : 0, 0.175, 0.5, 1.0, 2.5, 5.0 ng\/mL<\/p>\n

3. Antibody Solution (mouse anti-Caffeine), 6 mL<\/p>\n

4. Caffeine-HRP Conjugate Solution, 6 mL<\/p>\n

5. Diluent\/zero, 25 mL, use to dilute samples<\/p>\n

6. Wash Solution (5X) Concentrate, 100 mL, must be diluted before use<\/p>\n

7. Color Solution (TMB), 12 mL 8. Stop Solution, 12 mL<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716<\/p>\n

\"Creative<\/p>\n

Creative Diagnostics \u63d0\u4f9b\u7bc4\u570d\u5ee3\u6cdb\u7684\u9176\u806f\u514d\u75ab\u5438\u9644\u6e2c\u5b9a (ELISA) \u8a66\u5291\u76d2\uff0c\u7528\u65bc\u6aa2\u6e2c\u6578\u767e\u7a2e\u4e0d\u540c\u7684\u86cb\u767d\u8cea\u548c\u5206\u5b50\uff0c\u5305\u62ec\u7d30\u80de\u56e0\u5b50(cytokine)\u3001\u751f\u9577\u56e0\u5b50(\u00a0growth factors)\u3001\u50b3\u67d3\u75c5\u6a19\u8a8c\u7269(markers for infectious diseases)\u3001\u7cd6\u5c3f\u75c5\u548c\u816b\u7624(diabetes and tumor)\u3001\u85e5\u7269\u548c\u5c0f\u5206\u5b50\u7b49(\u00a0drugs and small molecules)\u3002ELISA \u8a66\u5291\u76d2 \u662f\u4e00\u7a2e\u5feb\u901f\u3001\u65b9\u4fbf\u548c\u6e96\u78ba\u7684\u7814\u7a76\u5de5\u5177\uff0c\u7528\u65bc\u6aa2\u6e2c\u548c\u5b9a\u91cf\u57f9\u990a\u7269\u548c\u6837\u54c1\u4e2d\u611f\u8208\u8da3\u7684\u9776\u6a19\u3002 \u6211\u5011\u63d0\u4f9b\u591a\u7a2e\u6aa2\u6e2c\u5f62\u5f0f\uff0c\u5305\u62ec\u50b3\u7d71\u593e\u5fc3 ELISA \u8a66\u5291\u76d2\u3001\u7af6\u722d\u6027 ELISA \u8a66\u5291\u76d2\uff0c\u4ee5\u53ca\u91dd\u5c0d\u8a31\u591a\u9776\u6a19\u7684\u57fa\u65bc\u7d30\u80de\u7684 ELISA \u8a66\u5291\u76d2\u3002 \u9019\u4e9b ELISA \u8a66\u5291\u76d2\u5305\u62ec\u5e36\u6709\u6355\u7372\/\u6aa2\u6e2c\u6297\u9ad4\u3001\u6a19\u6e96\u54c1\u3001\u7de9\u885d\u6db2\u548c\u8f14\u52a9\u8a66\u5291\u7684\u9810\u5305\u88ab\u677f\u3002 \u6bcf\u500b\u8a66\u5291\u76d2\u90fd\u7d93\u904e\u9069\u7528\u6027\u9a57\u8b49\u548c\u7a69\u5b9a\u6027\u6e2c\u8a66\uff0c\u4ee5\u78ba\u4fdd\u7cbe\u5bc6\u5ea6(precision)\u3001\u6e96\u78ba\u6027(accuracy)\u3001\u9748\u654f\u5ea6(sensitivity)\u3001\u7279\u7570\u6027(specificity)\u548c\u53ef\u91cd\u8907\u6027(reproducibility)\u3002<\/p>\n

\u60a8\u53ef\u4ee5\u6309\u76ee\u6a19\u540d\u7a31\u3001\u7269\u7a2e\u7b49\u641c\u7d22\u6240\u9700\u7684 ELISA \u8a66\u5291\u76d2\u3002\u5982\u679c\u60a8\u9700\u8981\u4efb\u4f55\u5e6b\u52a9\uff0c\u6211\u5011\u7d93\u9a57\u8c50\u5bcc\u7684\u79d1\u5b78\u5bb6\u975e\u5e38\u6a02\u610f\u63d0\u4f9b\u512a\u5316\u7684\u65b9\u6848\u548c\u6709\u7528\u7684\u6280\u8853\u652f\u6301\uff0c\u4ee5\u52a0\u901f\u60a8\u7684\u7814\u7a76\u3002<\/p>\n