{"id":38608,"date":"2023-02-20T15:47:20","date_gmt":"2023-02-20T07:47:20","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=38608"},"modified":"2023-02-27T10:42:07","modified_gmt":"2023-02-27T02:42:07","slug":"%e5%a4%a7%e9%bc%a0-alp%ef%bc%88%e9%b9%bc%e6%80%a7%e7%a3%b7%e9%85%b8%e9%85%b6%ef%bc%89elisa-%e8%a9%a6%e5%8a%91%e7%9b%92-elk-biotechnology%e8%b2%a8%e8%99%9felk5657","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=38608","title":{"rendered":"\u5927\u9f20 ALP\uff08\u9e7c\u6027\u78f7\u9178\u9176\uff09ELISA \u8a66\u5291\u76d2 -ELK Biotechnology\u8ca8\u865fELK5657"},"content":{"rendered":"

\u5927\u9f20 ALP\uff08\u9e7c\u6027\u78f7\u9178\u9176\uff09ELISA \u8a66\u5291\u76d2 -ELK Biotechnology\u8ca8\u865fELK5657<\/span><\/strong><\/a><\/p>\n

Rat ALP(Alkaline Phosphatase) ELISA Kit (Rat\u7269\u7a2e)<\/p>\n

\u9748\u654f\u5ea61.25-80 ng\/mL\u3001\u6700\u4f4e\u9748\u654f\u5ea60.53 ng\/mL<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong><\/span><\/p>\n

\u5927\u9f20 ALP\uff08\u9e7c\u6027\u78f7\u9178\u9176\uff09ELISA\u8a66\u5291\u76d2 | \u8ca8\u865fELK5657 (Sandwich ELISA)\uff0c\u5075\u6e2c\u7684\u9748\u654f\u5ea61.25-80 ng\/mL\u3001\u6700\u4f4e\u9748\u654f\u5ea60.53 ng\/mL\u3002ELISA\u539f\u7406\u70ba\u5229\u7528\u6297\u9ad4\u8207\u6297\u539f\u4e4b\u9593\u7684\u9ad8\u5ea6\u5c08\u4e00\u6027\uff0c\u505a\u70ba\u5b9a\u91cf\u6a19\u8a8c\u7684\u65b9\u6cd5\uff1b\u7121\u8ad6\u6e2c\u91cf\u6297\u539f\u6216\u6e2c\u91cf\u6297\u9ad4\u90fd\u6709\u5f88\u9ad8\u7684\u9748\u654f\u5ea6\u8ddf\u5c08\u4e00\u6027\u3002\u8a72\u6aa2\u6e2c\u65b9\u6cd5\u5c0d\u5927\u9f20 ALP\uff08\u9e7c\u6027\u78f7\u9178\u9176\uff09 \u5177\u6709\u9ad8\u9748\u654f\u5ea6\u548c\u51fa\u8272\u7684\u7279\u7570\u6027\u3002ELISA\u5546\u54c1\u7684\u578b\u5f0f\u591a\u5143\uff0c \u63d0\u4f9b\u4e09\u660e\u6cbbELISA\u3001\u7af6\u722d\u578bELISA\u3002\u53ef\u61c9\u7528\u65bcserum, plasma, tissue homogenates, cell lysates, cell culture\u3002\u4e26\u5728 450nm \u00b1 10nm \u7684\u6ce2\u9577\u4e0b\u7528\u5206\u5149\u5149\u5ea6\u8a08\u6e2c\u91cf\u984f\u8272\u8b8a\u5316\u3002\u7136\u5f8c\u901a\u904e\u5c07\u6a23\u54c1\u7684 OD \u8207\u6a19\u6e96\u66f2\u7dda\u9032\u884c\u6bd4\u8f03\u4f86\u78ba\u5b9a\u6a23\u54c1\u4e2d\u5927\u9f20 ALP\uff08\u9e7c\u6027\u78f7\u9178\u9176\uff09\u7684\u6fc3\u5ea6\u3002<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product name:<\/td>\nRat ALP(Alkaline Phosphatase) ELISA Kit<\/td>\n<\/tr>\n
Reactivity:<\/td>\nRat<\/td>\n<\/tr>\n
Alternative Names:<\/td>\nAKP; ALKP; Basic Phosphatase<\/td>\n<\/tr>\n
Assay Type:<\/td>\nSandwich<\/td>\n<\/tr>\n
Sensitivity:<\/td>\n0.53 ng\/mL<\/td>\n<\/tr>\n
Standard:<\/td>\n80 ng\/mL<\/td>\n<\/tr>\n
Range:<\/td>\n1.25-80 ng\/mL<\/td>\n<\/tr>\n
Sample Type:<\/td>\nserum, plasma, tissue homogenates and other biological fluids<\/td>\n<\/tr>\n
Assay Length:<\/td>\n3.5h<\/td>\n<\/tr>\n
Research Area:<\/td>\nEnzyme & Kinase;Metabolic pathway;Tumor immunity;Infection immunity;Hepatology;Bone metabolism;<\/td>\n<\/tr>\n
Test principle:<\/td>\nThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alkaline Phosphatase(ALP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alkaline Phosphatase(ALP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alkaline Phosphatase(ALP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm \u00b1 10nm. The concentration of Alkaline Phosphatase(ALP) in the samples is then determined by comparing the OD of the samples to the standard curve.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\"\"<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716<\/p>\n

ELK<\/strong>\u539f\u5ee0\u7db2\u5740<\/strong><\/span>\u00a0 >><\/span>\u00a0http:\/\/www.elkbiotech.com\/<\/a>\u00a0<\/span><\/strong><\/span><\/p>\n

ELK Biotechnology\u516c\u53f8\u662f\u4e00\u5bb6\u5c08\u6ce8\u65bc\u751f\u547d\u79d1\u5b78\u3001\u514d\u75ab\u7814\u7a76\u7684\u9ad8\u79d1\u6280\u751f\u7269\u4f01\u696d\uff0c\u4f4d\u65bcBiolake, Donghu New & High Technology Development Zone, Wuhan city.ELK Biotechnology\u662f\u4e00\u5bb6\u5c08\u6ce8\u65bc\u514d\u75ab\u7522\u54c1\u548c\u5206\u5b50\u751f\u7269\u5b78\u7522\u54c1\u7814\u767c\u548c\u751f\u7522\u7684\u9ad8\u79d1\u6280\u751f\u7269\u516c\u53f8\u3002\u7522\u54c1\u6db5\u84cbELISA\u8a66\u5291\u76d2\u3001\u6297\u9ad4\u3001\u57fa\u56e0\u7d44\u63d0\u53d6\u8a66\u5291\u3001\u5206\u5b50\u751f\u7269\u5b78\u8a66\u5291\u3002\u6d89\u53ca\u514d\u75ab\u5316\u5b78\u3001\u816b\u7624\u7814\u7a76\u3001\u795e\u7d93\u751f\u7269\u5b78\u3001\u7d30\u80de\u9031\u671f\u3001\u4fe1\u865f\u8f49\u5c0e\u7b49\u9818\u57df\u3002\u6211\u5011\u7684\u7522\u54c1\u6709\u56b4\u683c\u7684\u7814\u767c\u548c\u8cea\u91cf\u6aa2\u6e2c\u6d41\u7a0b\u3002\u6bcf\u500b\u7522\u54c1\u90fd\u6709\u5b8c\u6574\u7684\u7814\u767c\u3001\u751f\u7522\u548c\u8cea\u6aa2\u8a18\u9304\u3002\u7d93\u9a57\u8c50\u5bcc\u7684\u7814\u767c\u4eba\u54e1\uff0c\u5b8c\u5584\u7684\u8cea\u6aa2\u652f\u6301\u90e8\u9580\uff0c\u56b4\u683c\u7684\u6aa2\u6e2c\u6d41\u7a0b\uff0c\u70ba\u5ba2\u6236\u63d0\u4f9b\u8cea\u91cf\u53ef\u9760\u7684\u79d1\u7814\u7522\u54c1\u3002<\/p>\n

\u200b<\/span>\"ELK1.jpg\"\u200b<\/span><\/span>\u200b<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

\u5927\u9f20 ALP\uff08\u9e7c\u6027\u78f7\u9178\u9176\uff09ELISA \u8a66\u5291\u76d2 -ELK Biotechnology\u8ca8\u865fELK5657 Rat […]<\/p>\n","protected":false},"author":3,"featured_media":34915,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[452,3],"tags":[5318],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/38608"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=38608"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/38608\/revisions"}],"predecessor-version":[{"id":38633,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/38608\/revisions\/38633"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/34915"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=38608"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=38608"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=38608"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}