{"id":38374,"date":"2023-02-15T17:48:28","date_gmt":"2023-02-15T09:48:28","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=38374"},"modified":"2024-06-13T18:24:04","modified_gmt":"2024-06-13T10:24:04","slug":"astgot-%e6%b4%bb%e6%80%a7%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-chekine-micro-aspartate-aminotransferase-%e8%b2%a8%e8%99%9fktb1420","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=38374","title":{"rendered":"(AST\/GOT) \u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | CheKine\u2122 Micro Aspartate Aminotransferase \u8ca8\u865fKTB1420"},"content":{"rendered":"

(AST\/GOT) \u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | CheKine\u2122 Micro Aspartate Aminotransferase \u8ca8\u865fKTB1420<\/span><\/a><\/strong><\/span><\/p>\n

\u5929\u51ac\u6c28\u9178\u6c28\u57fa\u8f49\u79fb\u9176(Aspartate aminotransferase, AST\/GOT\uff09\u5ee3\u6cdb\u5b58\u5728\u65bc\u52d5\u7269\u3001\u690d\u7269\u3001\u5fae\u751f\u7269\u548c\u57f9\u990a\u7d30\u80de\u4e2d\u3002\u5b83\u50ac\u5316\u53ef\u9006\u7684\u6c28\u57fa\u53cd\u61c9(\u00a0reversible amino reaction )\uff0c\u662f\u6c28\u57fa\u9178\u4ee3\u8b1d(amino acid metabolism)\u7684\u91cd\u8981\u9176\u3002\u8840\u6e05AST\u6d3b\u6027(Serum AST activity)\u6c34\u5e73\u662f\u809d\u640d\u50b7(liver damage)\u7684\u91cd\u8981\u751f\u5316\u6307\u6a19(\u00a0biochemical indicator )\uff0c\u53ef\u4ee5\u53cd\u6620\u809d\u640d\u50b7(liver damage)\u7684\u56b4\u91cd\u7a0b\u5ea6\u3002\u6b64\u5916\uff0c\u5929\u51ac\u6c28\u9178\u6c28\u57fa\u8f49\u79fb\u9176(Aspartate aminotransferase, AST\/GOT\uff09\u5728\u5fc3\u808c\u7d30\u80de\u4e2d\u542b\u91cf\u6700\u9ad8\uff0c\u81e8\u5e8a\u4e0a\u4e00\u822c\u4f5c\u70ba\u5fc3\u808c\u6897\u585e(cardiomyocytes)\u3001\u5fc3\u808c\u708e(\u00a0myocardial infarction)\u7684\u8f14\u200b\u200b\u52a9\u6aa2\u67e5\u3002\u8a72\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u6aa2\u6e2c\u52d5\u7269\u7d44\u7e54\u3001\u690d\u7269\u7d44\u7e54\u3001\u7d30\u80de\u57f9\u990a\u7269\uff1a\u8cbc\u58c1\u6216\u61f8\u6d6e\u7d30\u80de\u3001\u8840\u6e05\u3001\u7d30\u83cc\u7b49\u591a\u7a2e\u751f\u7269\u6a23\u54c1\u4e2d AST\/GOT \u6d3b\u6027\u7684\u7c21\u55ae\u65b9\u6cd5\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0c\u5929\u51ac\u6c28\u9178\u6c28\u57fa\u8f49\u79fb\u9176 (AST) \u5728 37\u00b0C \u548c pH 7.4 \u4e0b\u50ac\u5316 \u03b1-\u916e\u620a\u4e8c\u9178(\u03b1-ketoglutarate)\u548c L-\u5929\u51ac\u6c28\u9178(L-aspartic acid )\u7684\u8f49\u6c28\u57fa\u53cd\u61c9(transamination reaction)\uff0c\u751f\u6210\u8c37\u6c28\u9178(\u00a0glutamic acid)\u548c\u4e19\u916e\u9178(pyruvate)\uff1b\u4e19\u916e\u9178(pyruvate)\u80fd\u82072,4-\u4e8c\u785d\u57fa\u82ef\u80bc(\u00a02,4- Dinitrophenylhydrazine )\u4f5c\u7528\u751f\u6210\u4e19\u916e\u9178\u82ef\u8159(pyruvate phenylhydrazone)\uff0c\u5728\u9e7c\u6027\u689d\u4ef6(\u00a0alkaline conditions )\u4e0b\u5448\u68d5\u7d05\u8272\uff0c\u5728505nm\u8655\u6709\u7279\u5fb5\u5438\u6536\u5cf0\u3002\u4e19\u916e\u9178\u82ef\u8159(pyruvate phenylhydrazone)\u5728 505 nm \u8655\u7684\u589e\u52a0\u901f\u7387\u53ef\u4ee5\u53cd\u6620 AST\/GOT \u6d3b\u6027\u3002<\/p>\n

<\/p>\n

\u5f15\u7528\u6587\u737b\u00a0<\/a><\/span><\/p>\n

<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nCheKine\u2122 Micro Aspartate Aminotransferase (AST\/GOT) Activity Assay Kit<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nThe kit provides a simple method for detecting AST\/GOT activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial. In the assay, Aspartate aminotransferase (AST) catalyzes the transamination reaction of \u03b1-ketoglutarate and L-aspartic acid at 37\u00b0C and pH 7.4 to produce glutamic acid and pyruvate; Pyruvate can interact with 2,4- Dinitrophenylhydrazine to produce pyruvate phenylhydrazone, which appears brownish red under alkaline conditions and has a characteristic absorption peak at 505 nm. The rate of pyruvate phenylhydrazone increase at 505 nm can reflect AST\/GOT activity.<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nAST; GOT; Aspartate aminotransferase<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 Extraction Buffer
\n\u2022 Reagent I
\n\u2022 Reagent II
\n\u2022 Reagent III
\n\u2022 Standard<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Determination of AST\/GOT activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial.
\n\u2022 Detailed sample preparation and result calculation methods are provided.
\n\u2022 Simple operation steps.
\n\u2022 Standard and standard curve line are provided.<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\n\u2022 Do not mix or substitute reagents or materials from other kit lots or vendors.
\n\u2022 The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw.
\n\u2022 Ensure all reagents and equipment are at the appropriate temperature before starting the assay.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStorage at 4\u00b0C and protected from light upon receipt. Kit has a storage time of 6 months form receipt.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n
Background<\/strong><\/td>\nAspartate aminotransferase (AST\/GOT) is widely present in animals, plants, microorganisms and cultured cells. It catalyzes the reversible amino reaction and is an important enzyme for amino acid metabolism. Serum AST activity level is an important biochemical indicator of liver damage, which can reflect the severity of liver damage. In addition, AST has the highest content in cardiomyocytes and is generally used as an auxiliary examination for myocardial infarction and myocarditis in clinical practice.<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nAST; GOT; Aspartate aminotransferase<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

(AST\/GOT) \u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | CheKine\u2122 Micro Aspartate Aminotrans […]<\/p>\n","protected":false},"author":3,"featured_media":28512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262],"tags":[5336],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/38374"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=38374"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/38374\/revisions"}],"predecessor-version":[{"id":48797,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/38374\/revisions\/48797"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28512"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=38374"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=38374"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=38374"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}