{"id":38358,"date":"2023-02-14T17:35:51","date_gmt":"2023-02-14T09:35:51","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=38358"},"modified":"2023-02-27T10:45:47","modified_gmt":"2023-02-27T02:45:47","slug":"%e7%b4%b0%e8%83%9e%e5%87%8b%e4%ba%a1%e7%b3%bb%e5%88%97%e5%95%86%e5%93%81-annexin-v-fitc-apoptosis-kit-%e8%b2%a8%e8%99%9fabcam-ab14085","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=38358","title":{"rendered":"\u7d30\u80de\u51cb\u4ea1\u7cfb\u5217\u5546\u54c1- Annexin V-FITC Apoptosis Kit | \u8ca8\u865fabcam ab14085"},"content":{"rendered":"

\u7d30\u80de\u51cb\u4ea1\u7cfb\u5217\u5546\u54c1-\u00a0Annexin V-FITC Apoptosis Kit\u00a0\u00a0| \u8ca8\u865f<\/a>abcam ab14085<\/span><\/strong><\/span><\/p>\n

\u7d30\u80de\u51cb\u4ea1\u662f\u6307\u70ba\u7dad\u6301\u6709\u6a5f\u9ad4\u5167\u74b0\u5883\u7a69\u5b9a\uff0c\u7531\u57fa\u56e0\u63a7\u5236\u7684\u7d30\u80de\u81ea\u4e3b\u7684\u6709\u5e8f\u7684\u6b7b\u4ea1\u3002\u6b63\u5e38\u60c5\u6cc1\u4e0b\uff0c\u4efb\u4f55\u7d30\u80de\u5728\u5f62\u6210\u904e\u7a0b\u4e2d\u767c\u751f\u7684\u7570\u5e38\u90fd\u6703\u901a\u904e\u51cb\u4ea1\u6d88\u9664\u3002\u4f8b\u5982\uff0c\u9ad4\u5167\u7684\u764c\u7d30\u80de\u589e\u9577\u70ba\u816b\u7624\u7684\u904e\u7a0b\u6703\u53d7\u7d30\u80de\u51cb\u4ea1\u7684\u5f15\u5c0e\u800c\u88ab\u6291\u5236\u3002\u7136\u800c\uff0c\u5728\u6291\u764c\u57fa\u56e0p53\u51fa\u73fe\u554f\u984c\u6642\uff0c\u51cb\u4ea1\u5c31\u4e0d\u6703\u8a98\u5c0e\u767c\u751f\uff0c\u5f9e\u800c\u5c0e\u81f4\u764c\u7d30\u80de\u7684\u4e0d\u65b7\u589e\u9577\u3002\u7d30\u80de\u51cb\u4ea1\u53ef\u4ee5\u901a\u904e\u7d30\u80de\u5f62\u614b\u7684\u8b8a\u5316\u6216\u751f\u7269\u5316\u5b78\u7684\u8b8a\u5316\u4f86\u6aa2\u6e2c\u3002\u76ee\u524d\u5e38\u7528\u7684\u6307\u6a19\u6709caspase\u6d3b\u6027\u5206\u6790\u3001DNA\u7247\u6bb5\u5206\u6790\u3001\u53ca\u78f7\u8102\u91af\u7d72\u6c28\u9178phosphatidylserine (PS)\u7684\u5916\u7ffb\u7b49\u5be6\u9a57\u3002<\/p>\n

\"\"<\/p>\n

\u5c0f\u9f20\u76ae\u8cea\u96c6\u5408\u7ba1\u7d30\u80de\u7684\u51cb\u4ea1<\/strong><\/span><\/div>\n
\n

Ab14085 \u7528\u65bc\u78ba\u5b9a\u5c0f\u9f20\u76ae\u8cea\u96c6\u5408\u7ba1\u7d30\u80de (mCCD) \u4e2d\u7684\u6b21\u8981\u6c34\u5e73\u7684\u7d30\u80de\u51cb\u4ea1\uff08\u4f7f\u7528 Annexin V-FITC \u548c PI\uff09\u3002mCCD \u7d30\u80de\u8207\u7121\u8840\u6e05\u57f9\u990a\u57fa\u4e00\u8d77\u5b75\u80b2 48 \u5c0f\u6642\u3002\u8cea\u819c\u4e0a\u7684\u7da0\u8272\u6a19\u7c64 (Annexin V-FITC) \u548c\u6838\u7d05 (PI) \u67d3\u8272\u7684\u7f3a\u5931\u8868\u660e\u7d30\u80de\u51cb\u4ea1\u800c\u975e\u58de\u6b7b\u3002\u7192\u5149\u986f\u5fae\u93e1\u7528\u65bc\u5206\u6790\u7d30\u80de\u3002<\/p>\n<\/div>\n

Annexin V \u7d30\u80de\u51cb\u4ea1\u5075\u6e2c\u539f\u7406<\/strong><\/span><\/p>\n

Annexin V\u7d30\u80de\u67d3\u8272\uff0c\u7528\u65bc\u6aa2\u6e2c\u7d30\u80de\u51cb\u4ea1\u65e9\u671f\u7684\u7d30\u80de\u819c\u8b8a\u5316\u3002\u5728\u7d30\u80de\u51cb\u4ea1\u65e9\u671f\uff0c\u819c\u78f7\u8102\u91af\u7d72\u6c28\u9178phosphatidylserine (PS)\u7531\u8102\u819c\u5167\u5074\u7ffb\u5411\u5916\u5074\u3002Annexin V\u662f\u4e00\u7a2eCa\u96e2\u5b50\u4f9d\u8cf4\u6027\u78f7\u8102\u7d50\u5408\u86cb\u767d\uff0c\u53ef\u4ee5\u8207\u78f7\u8102\u91af\u7d72\u6c28\u9178phosphatidylserine (PS) \u7279\u7570\u7d50\u5408\u3002\u7528\u7da0\u8272\u87a2\u5149FITC\u6a19\u8a18\u7684Annexin V\u901a\u904e\u6d41\u5f0f\u7d30\u80de\u5100\u6216\u87a2\u5149\u986f\u5fae\u93e1\u53ef\u4ee5\u6aa2\u6e2c\u5230\u7d30\u80de\u51cb\u4ea1\u7684\u767c\u751f\u3002\u7d50\u5408\u7d30\u80de\u6838\u67d3\u5291PI\uff0c\u5229\u7528\u6d41\u5f0f\u7d30\u80de\u5100\u6216\u87a2\u5149\u986f\u5fae\u93e1\u53ef\u5075\u6e2c\u5075\u6e2c\u7d30\u80de\u51cb\u4ea1\u73fe\u8c61\u53ca\u5176\u51cb\u4ea1\u5206\u671f\u3002\u7898\u5316\u4e19\u5576(Propidium Iodide, PI)\u662f\u4e00\u7a2e\u6838\u9178\u67d3\u6599\uff0cPI\u53ea\u80fd\u900f\u904e\u51cb\u4ea1\u665a\u671f\u548c\u6b7b\u7d30\u80de\u7684\u7d30\u80de\u819c\uff0c\u56e0\u6b64Annexin V\u548cPI\u7d50\u5408\u4f7f\u7528\uff0c\u53ef\u4ee5\u5340\u5206\u51cb\u4ea1\u65e9\u665a\u671f\u7684\u7d30\u80de\u53ca\u6b7b\u7d30\u80de\u3002<\/p>\n

\u7522\u54c1\u4ecb\u7d39:<\/strong>\u00a0<\/span><\/p>\n

Annexin V-FITC Apoptosis Kit\u63d0\u4f9b\u4e00\u500b\u6b65\u9a5f\u3001\u5feb\u901f\u5206\u6790\u7d30\u80de\u65e9\u671f\u51cb\u4ea1\u73fe\u8c61\u3002\u5957\u7d44\u5167\u5305\u62ec\u6709Annexin V-FITC\u53caPropidium Iodide \u3002PI\u96d9\u67d3\u5291\uff1b\u7da0\u8272\u87a2\u5149FITC\u6a19\u8a18\u7684Annexin V\u901a\u904e\u6d41\u5f0f\u7d30\u80de\u5100\u6216\u87a2\u5149\u986f\u5fae\u93e1\u53ef\u4ee5\u6aa2\u6e2c\u5230\u7d30\u80de\u51cb\u4ea1\u7684\u767c\u751f\u3002\u7d50\u5408\u7d30\u80de\u6838\u67d3\u5291PI\uff0c\u5229\u7528\u6d41\u5f0f\u7d30\u80de\u5100\u6216\u87a2\u5149\u986f\u5fae\u93e1\u53ef\u5075\u6e2c\u5075\u6e2c\u7d30\u80de\u51cb\u4ea1\u73fe\u8c61\u53ca\u5176\u51cb\u4ea1\u5206\u671f\u3002\u7898\u5316\u4e19\u5576(Propidium Iodide, PI)\u662f\u4e00\u7a2e\u6838\u9178\u67d3\u6599\uff0cPI\u53ea\u80fd\u900f\u904e\u51cb\u4ea1\u665a\u671f\u548c\u6b7b\u7d30\u80de\u7684\u7d30\u80de\u819c\uff0c\u56e0\u6b64Annexin V\u548cPI\u7d50\u5408\u4f7f\u7528\uff0c\u53ef\u4ee5\u5340\u5206\u51cb\u4ea1\u65e9\u665a\u671f\u7684\u7d30\u80de\u53ca\u6b7b\u7d30\u80de\u3002Biovision\u63d0\u4f9bFITC\u3001Cy3\u3001EGFP\u3001Cy5\u3001PE\u3001Biotin\u7b49\u591a\u7a2eAnnexin V\u6a19\u8a8c\u8a66\u5291\/\u5957\u7d44\uff0c\u5728\u5be6\u9a57\u9078\u64c7\u4e0a\u5177\u8f03\u591a\u9078\u64c7\u3002\u53e6\u5177\u670925\u3001100\u3001400\u500b\u5206\u6790\u5305\u88dd\u5957\u7d44\u3002<\/p>\n

Annexin-V<\/b><\/span><\/span>\u76f8\u95dc\u5546\u54c1<\/b><\/span><\/span>\u00a0<\/b>Annexin-V FITC<\/b><\/span>\u5206\u6790\u5957\u7d44<\/b><\/span><\/span><\/a>\u00a0|\u00a0<\/b><\/span>Annexin-V Cy3<\/b><\/span>\u5206\u6790\u5957\u7d44<\/b><\/span><\/span><\/a>\u00a0|\u00a0<\/b><\/span>Annexin-V PE<\/b><\/span>\u5206\u6790\u5957\u7d44<\/b><\/span><\/span><\/a>\u00a0|\u00a0<\/b><\/span>Annexin-V FITC<\/b><\/span>\u8a66\u5291<\/b><\/span><\/span><\/a>\u00a0|\u00a0<\/b><\/span>Annexin-V Cy5<\/b><\/span>\u8a66\u5291<\/b><\/span><\/span><\/a>\u00a0|\u00a0Annexin-V Binding Buffer<\/a>\u00a0|\u00a0<\/b><\/span>Propidium Iodide<\/b><\/span><\/b><\/a><\/b><\/span><\/span><\/p>\n

\"\"<\/p>\n

\u8655\u7406 48 \u5c0f\u6642\u7684 HeLa \u7d30\u80de\u7684\u6d41\u5f0f\u7d30\u80de\u8853\u5206\u6790<\/strong><\/span><\/div>\n
\n

HeLa \u7d30\u80de\u901a\u904e\u80f0\u86cb\u767d\u9176\u6d88\u5316\u8207\u6f02\u6d6e\u7684\u975e\u6d3b\u7d30\u80de\u4e00\u8d77\u6536\u7a6b\u3002\u7d30\u80de\u7528 PBS \u6d17\u6ecc\u4e26\u5728\u5206\u6790\u524d 20 \u5206\u9418\u61f8\u6d6e\u5728\u6ab8\u6aac\u9178\u9209\u7de9\u885d\u6db2\u4e2d\u3002HeLa \u7d30\u80de\u7528\u7c73\u6258\u84bd\u918c (MX) \u548c MX + \u4f0a\u99ac\u66ff\u5c3c\u8655\u7406 48 \u5c0f\u6642\u3002\u7136\u5f8c\u7528\u819c\u806f\u86cb\u767d V-FITC \u7d30\u80de\u51cb\u4ea1\u67d3\u8272\/\u6aa2\u6e2c\u8a66\u5291\u76d2 (ab14085) \u5c0d\u6a23\u54c1\u9032\u884c\u67d3\u8272\u3002FACSCalibur \u6d41\u5f0f\u7d30\u80de\u5100\u7528\u65bc\u7d30\u80de\u9031\u671f\u5206\u6790\u3002<\/p>\n

\"\"<\/p>\n

CTA095\u8655\u7406\u5f8c\u524d\u5217\u817a\u764c\u7d30\u80de\u51cb\u4ea1\u5206\u6790<\/strong><\/span><\/div>\n
\"\"<\/div>\n
<\/div>\n
\u529f\u80fd\u7814\u7a76 – Annexin V-FITC \u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2 (ab14085)<\/span><\/strong><\/div>\n
\n

PC3 \u7d30\u80de\u4ee5 10\u00a06 \u500b<\/sup>\u7d30\u80de\/ml \u63a5\u7a2e\u4e26\u5b75\u80b2\u904e\u591c\uff0c\u7136\u5f8c\u7528\u4e0d\u540c\u6fc3\u5ea6\u7684 CTA095 \u8655\u7406 24 \u5c0f\u6642\u3002\u7136\u5f8c\u4f7f\u7528 Annexin-V FITC \u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2 (ab14085) \u5206\u6790\u7d30\u80de\u51cb\u4ea1\u3002<\/p>\n<\/div>\n<\/div>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u7d30\u80de\u51cb\u4ea1\u7cfb\u5217\u5546\u54c1-\u00a0Annexin V-FITC Apoptosis Kit\u00a0\u00a0| \u8ca8\u865fabcam ab140 […]<\/p>\n","protected":false},"author":3,"featured_media":37625,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[5337],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/38358"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=38358"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/38358\/revisions"}],"predecessor-version":[{"id":38360,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/38358\/revisions\/38360"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/37625"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=38358"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=38358"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=38358"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}