{"id":38216,"date":"2023-02-09T14:26:12","date_gmt":"2023-02-09T06:26:12","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=38216"},"modified":"2023-02-14T13:09:16","modified_gmt":"2023-02-14T05:09:16","slug":"t4-dna%e9%80%a3%e6%8e%a5%e9%85%b6-dna-ligase%e8%b2%a8%e8%99%9fg467","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=38216","title":{"rendered":"T4 DNA\u9023\u63a5\u9176 | DNA Ligase\u8ca8\u865fG467"},"content":{"rendered":"

\"\"T4 DNA\u9023\u63a5\u9176 | DNA Ligase\u8ca8\u865fG467<\/a>\u00a01 U\/\u03bcl 200 U (200 \u03bcl)<\/span><\/span><\/p>\n

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3\u2019- hydroxyl and 5\u2019- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated interdiate, this endergonic reaction can be utilized to join both cohesive-ended and blunt-ended termini.<\/p>\n

\"\"<\/p>\n

T4 DNA \u9023\u63a5\u9176(T4 DNA Ligase)\u50ac\u5316\u5728 dsDNA\u3001dsRNA \u6216 DNA-RNA \u96dc\u4ea4\u9ad4\u7684\u76f8\u9130\u5207\u53e3\u6838\u82f7\u9178\u7684\u8fd1\u7aef 3′- \u7fa5\u57fa\u548c 5′- \u78f7\u9178\u672b\u7aef\u4e4b\u9593\u5f62\u6210\u78f7\u9178\u4e8c\u916f\u9375(\u00a0phosphodiester bond )\u3002\u5df2\u77e5\u901a\u904e\u817a\u82f7\u9178\u5316\u4e2d\u9593\u9ad4(adenylated intermediate)\u9032\u884c\uff0c\u9019\u7a2eendergonic reaction\u53ef\u7528\u65bc\u9023\u63a5\u5167\u805a\u672b\u7aef(\u00a0cohesive-ended )\u548c\u5e73\u672b\u7aef(\u00a0blunt-ended termini)\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n
Description<\/th>\nT4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3\u2019- hydroxyl and 5\u2019- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated intermediate, this endergonic reaction can be utilized to join both cohesive-ended and blunt-ended termini.<\/td>\n<\/tr>\n
SKU<\/th>\nG467<\/td>\n<\/tr>\n
Unit quantity<\/th>\n200 U (200 \u03bcl)<\/td>\n<\/tr>\n
Applications<\/th>\n\u2022 Cloning of restriction fragments
\n\u2022 Attaching double-stranded oligonucleotide linkers or adaptors to DNA
\n\u2022 Site-directed mutagenesis
\n\u2022 Nick repair of duplex DNA, RNA or DNA-RNA hybrids
\n\u2022 Self-circularization of linear DNA<\/td>\n<\/tr>\n
Concentration<\/th>\n1 U\/\u03bcl<\/td>\n<\/tr>\n
Format<\/th>\nEnzyme supplied with 5X Reaction Buffer<\/td>\n<\/tr>\n
Storage Condition<\/th>\nStore all components at -20\u00b0C.<\/td>\n<\/tr>\n
Note<\/th>\nThis product is distributed for laboratory research only. Caution: Not for diagnostic use.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

T4 DNA\u9023\u63a5\u9176 | DNA Ligase\u8ca8\u865fG467\u00a01 U\/\u03bcl 200 U (200 \u03bcl) T4 D […]<\/p>\n","protected":false},"author":3,"featured_media":28511,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[5246],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/38216"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=38216"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/38216\/revisions"}],"predecessor-version":[{"id":38242,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/38216\/revisions\/38242"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28511"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=38216"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=38216"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=38216"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}