{"id":38094,"date":"2023-02-08T12:37:21","date_gmt":"2023-02-08T04:37:21","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=38094"},"modified":"2025-03-03T14:31:07","modified_gmt":"2025-03-03T06:31:07","slug":"fibronectin-fn%e6%b4%bb%e6%80%a7%e9%87%8d%e7%b5%84%e8%9b%8b%e7%99%bd-active-fibronectin-fn%e8%b2%a8%e8%99%9fapa037hu02","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=38094","title":{"rendered":"Fibronectin (FN)\u6d3b\u6027\u91cd\u7d44\u86cb\u767d | Active Fibronectin (FN)\u8ca8\u865fAPA037Hu02"},"content":{"rendered":"<p><span style=\"font-size: 12pt; color: #33cccc;\"><a style=\"color: #33cccc;\" href=\"http:\/\/biopioneer.com.tw\/?p=38094\">Fibronectin (FN)\u6d3b\u6027\u91cd\u7d44\u86cb\u767d | Active Fibronectin (FN)\u8ca8\u865fAPA037Hu02<\/a><\/span><\/p>\n<p>FN1; CIG; FINC; LETS; MSF; GFND2; Anastellin; Migration-Stimulating Factor; Cold-Insoluble Globulin; Large, External, Transformation-Sensitive Protein<\/p>\n<p><img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/02\/Fibronectin-FN%E6%B4%BB%E6%80%A7%E9%87%8D%E7%B5%84%E8%9B%8B%E7%99%BD-Active-Fibronectin-FN%E8%B2%A8%E8%99%9FAPA037Hu02-2.jpg\" alt=\"\" \/><\/p>\n<p><a href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/02\/Fibronectin-FN%E6%B4%BB%E6%80%A7%E9%87%8D%E7%B5%84%E8%9B%8B%E7%99%BD-Active-Fibronectin-FN%E8%B2%A8%E8%99%9FAPA037Hu021.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/01\/%E7%B6%B2%E7%AB%99%E8%A6%8F%E6%A0%BC%E5%B0%8F%E5%9C%96_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-11-300x97.jpg\" alt=\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df 1_\u5de5\u4f5c\u5340\u57df 1\" width=\"149\" height=\"48\" \/><\/a><\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>\u7522\u54c1\u4ecb\u7d39<\/strong><strong>:<\/strong> <\/span>Cloud clone\u63d0\u4f9b\u539f\u6838\u53ca\u771f\u6838\u751f\u7269\u8868\u73fe\u4e4b\u91cd\u7d44\u86cb\u767d\u7cfb\u5217\u5546\u54c1\uff1b\u5176\u4e2dActive Fibronectin (FN)\u6d3b\u6027\u91cd\u7d44\u86cb\u767d\uff0c\u70ba\u6d3b\u6027\u86cb\u767d\uff0cOrganism Species<strong>\u00a0<\/strong>Homo sapiens (Human)\u00a0\u3002\u7d14\u5ea6&gt; 90%\uff0c\u63d0\u4f9b\u51cd\u4e7e\u578b\u5f0f\u5305\u88dd(Freeze-dried powder)\u3002\u53ef\u61c9\u7528\u65bcCell culture; Activity Assays\u7b49\u4e0d\u540c\u7814\u7a76\u3002<\/p>\n<p><img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/02\/Fibronectin-FN%E6%B4%BB%E6%80%A7%E9%87%8D%E7%B5%84%E8%9B%8B%E7%99%BD-Active-Fibronectin-FN%E8%B2%A8%E8%99%9FAPA037Hu02.jpg\" alt=\"\" \/><\/p>\n<p><img decoding=\"async\" src=\"http:\/\/de.static.cloud-clone.com\/product\/pictures\/RPA551Po01-201912101538067538.png\" alt=\"\" \/><\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>\u86cb\u767d\u8cea\u4fdd\u5b58\u578b\u5f0f<\/strong><\/span><\/p>\n<p>\u86cb\u767d\u8cea\u6eb6\u65bc20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.\u70ba\u51b7\u51cd\u4e7e\u71e5\u5305\u88dd\u3002\u4f7f\u7528\u524dReconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg\/mL. Do not vortex.\u907f\u514dvortex. \u77ed\u671f\u4fdd\u5b58Store at 2-8\u00b0C for one month.\u5206\u88dd-80\u00b0C\u9577\u671f12 \u500b\u6708\u3002\u8acb\u907f\u514d\u53cd\u8986\u51b7\u51cd\u89e3\u51cd\u3002<\/p>\n<p>\u7e96\u9023\u86cb\u767d (Fibronectin , FN) \u662f\u7d30\u80de\u5916\u57fa\u8cea(extracellular matrix)\u7684\u4e00\u7a2e\u9ad8\u5206\u5b50\u91cf (~440kDa) \u7cd6\u86cb\u767d(glycoprotein)\uff0c\u53ef\u8207\u7a31\u70ba\u6574\u5408\u7d20(integrins)\u7684\u8de8\u819c\u53d7\u9ad4\u86cb\u767d(membrane-spanning receptor proteins)\u7d50\u5408\u3002\u7e96\u9023\u86cb\u767d(Fibronectins, FN)\u7d50\u5408\u7d30\u80de\u8868\u9762(cell surfaces)\u548c\u5404\u7a2e\u5316\u5408\u7269(various compounds)\uff0c\u5305\u62ec\u81a0\u539f\u86cb\u767d(collagen)\u3001\u7e96\u7dad\u86cb\u767d(fibrin)\u3001\u809d\u7d20(heparin)\u3001DNA \u548c\u808c\u52d5\u86cb\u767d\u3002\u7e96\u9023\u86cb\u767d(Fibronectins, FN)\u5177\u6709\u591a\u7a2e\u529f\u80fd\u3002\u4f8b\u5982\uff0c\u5b83\u53c3\u8207\u7d30\u80de\u7c98\u9644( cell adhesion)\u3001\u7d30\u80de\u904b\u52d5(cell motility)\u3001\u8abf\u7406\u4f5c\u7528(opsonization)\u3001\u50b7\u53e3\u7652\u5408(wound healing)\u3001\u7d30\u80de\u5f62\u614b\u7dad\u6301(maintenance of cell shape)\u7b49\u3002\u6b64\u5916\uff0c\u7d50\u7de0\u7d44\u7e54\u751f\u9577\u56e0\u5b50 (CTGF) \u5df2\u88ab\u78ba\u5b9a\u70ba \u7e96\u9023\u86cb\u767d(Fibronectins, FN)\u7684\u76f8\u4e92\u4f5c\u7528\u56e0\u5b50\uff0c\u56e0\u6b64\u9032\u884c\u7d50\u5408 ELISA \u6e2c\u5b9a\u4ee5\u6aa2\u6e2c\u91cd\u7d44\u4eba\u7e96\u9023\u86cb\u767d(Fibronectins, FN) \u548c\u91cd\u7d44\u4eba CTGF \u7684\u76f8\u4e92\u4f5c\u7528\u3002\u7c21\u800c\u8a00\u4e4b\uff0c\u5c07 \u7e96\u9023\u86cb\u767d(Fibronectins, FN) \u5728\u542b\u6709 0.01% BSA (pH 7.4) \u7684 PBS \u4e2d\u9023\u7e8c\u7a00\u91cb\u3002\u7136\u5f8c\u5c07 100uL \u7684\u91cd\u8907\u6a23\u54c1\u8f49\u79fb\u5230 CTGF \u5305\u88ab\u7684\u5fae\u91cf\u6ef4\u5b9a\u5b54\u4e2d\uff0c\u4e26\u5728 37\u2103 \u4e0b\u5b75\u80b2 2 \u5c0f\u6642\u3002\u7528PBST\u6d17\u6ecc\u5b54\u4e26\u7528\u6297\u7e96\u9023\u86cb\u767d(Fibronectins, FN)pAb\u5b75\u80b21\u5c0f\u6642\uff0c\u7136\u5f8c\u5438\u51fa\u4e26\u6d17\u6ecc3\u6b21\u3002\u7528HRP\u6a19\u8a18\u7684\u4e8c\u6297\u5b75\u80b2\u5f8c\uff0c\u5c07\u5b54\u5438\u51fa\u4e26\u6d17\u6ecc3\u6b21\u3002\u52a0\u5165\u5e95\u7269\u6eb6\u6db2\uff0c37\u2103\u5b75\u80b215-25\u5206\u9418\u3002\u6700\u5f8c\uff0c\u5411\u5b54\u4e2d\u52a0\u5165 50\u00b5L \u7d42\u6b62\u6db2\uff0c\u7acb\u5373\u5728 450nm \u8655\u8b80\u6578\u3002 FN\u8207CTGF\u7684\u7d50\u5408\u6d3b\u6027\u5982\u57161\u6240\u793a\uff0c\u9019\u7a2e\u4f5c\u7528\u5448\u5291\u91cf\u4f9d\u8cf4\u6027\u3002<\/p>\n<ul>\n<li><b>Product No.<\/b><span id=\"post_number\">APA037Hu02<\/span><\/li>\n<li><b>Organism Species<\/b>Homo sapiens (Human)\u00a0<i id=\"name_specie\">Same name, Different species.<\/i><\/li>\n<li><b>Buffer Formulation<\/b>PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.<\/li>\n<li class=\"product_pic_position\"><b>Traits<\/b>Freeze-dried powder<\/li>\n<li><b>Purity<\/b>&gt; 90%<\/li>\n<li><b>Isoelectric Point<\/b>9.1<\/li>\n<li><b>Applications<\/b>Cell\u00a0culture;\u00a0Activity\u00a0Assays.<\/li>\n<li><b>Download<\/b><a href=\"https:\/\/cloud-clone.com\/manual\/Active-Fibronectin-(FN)-APA037Hu02.pdf\">Instruction Manual<\/a><\/li>\n<\/ul>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>Fibronectin (FN)<\/strong><strong>\u6d3b\u6027<\/strong><strong>\u91cd\u7d44\u86cb\u767d<\/strong><strong>\u76f8\u95dc\u4e4b\u6297\u9ad4<\/strong><strong> | <\/strong><strong>\u86cb\u767d\u8cea<\/strong> <strong>\u7522\u54c1<\/strong><\/span><\/p>\n<table>\n<tbody>\n<tr class=\"first_tr\">\n<td class=\"td_1\">Catalog No.<\/td>\n<td class=\"td_2\">Related products for research use of Homo sapiens (Human) Organism species<\/td>\n<td class=\"td_3\">Applications (RESEARCH USE ONLY!)<\/td>\n<\/tr>\n<tr>\n<td>APA037Hu02<\/td>\n<td><a href=\"http:\/\/www.cloud-clone.com\/products\/APA037Hu02.html\">Active Fibronectin (FN)<\/a><\/td>\n<td>Cell\u00a0culture;\u00a0Activity\u00a0Assays.<\/td>\n<\/tr>\n<tr>\n<td>RPA037Hu03<\/td>\n<td><a href=\"http:\/\/www.cloud-clone.com\/products\/RPA037Hu03.html\">Recombinant Fibronectin (FN)<\/a><\/td>\n<td>Positive Control; Immunogen; SDS-PAGE; WB.<\/td>\n<\/tr>\n<tr>\n<td>APA037Hu61<\/td>\n<td><a href=\"http:\/\/www.cloud-clone.com\/products\/APA037Hu61.html\">Active Fibronectin (FN)<\/a><\/td>\n<td>Cell\u00a0culture;\u00a0Activity\u00a0Assays.<\/td>\n<\/tr>\n<tr>\n<td>EPA037Hu61<\/td>\n<td><a href=\"http:\/\/www.cloud-clone.com\/products\/EPA037Hu61.html\">Eukaryotic Fibronectin (FN)<\/a><\/td>\n<td>Positive Control; Immunogen; SDS-PAGE; WB.<\/td>\n<\/tr>\n<tr>\n<td>APA037Hu01<\/td>\n<td><a href=\"http:\/\/www.cloud-clone.com\/products\/APA037Hu01.html\">Active Fibronectin (FN)<\/a><\/td>\n<td>Cell\u00a0culture;\u00a0Activity\u00a0Assays.<\/td>\n<\/tr>\n<tr>\n<td>RPA037Hu01<\/td>\n<td><a href=\"http:\/\/www.cloud-clone.com\/products\/RPA037Hu01.html\">Recombinant Fibronectin (FN)<\/a><\/td>\n<td>Positive Control; Immunogen; SDS-PAGE; WB.<\/td>\n<\/tr>\n<tr>\n<td>RPA037Hu02<\/td>\n<td><a href=\"http:\/\/www.cloud-clone.com\/products\/RPA037Hu02.html\">Recombinant Fibronectin (FN)<\/a><\/td>\n<td>Positive Control; Immunogen; SDS-PAGE; WB.<\/td>\n<\/tr>\n<tr>\n<td>PAA037Hu01<\/td>\n<td><a href=\"http:\/\/www.cloud-clone.com\/products\/PAA037Hu01.html\">Polyclonal Antibody to Fibronectin (FN)<\/a><\/td>\n<td>WB; IHC; ICC; IP.<\/td>\n<\/tr>\n<tr>\n<td>PAA037Hu04<\/td>\n<td><a href=\"http:\/\/www.cloud-clone.com\/products\/PAA037Hu04.html\">Polyclonal Antibody to Fibronectin (FN)<\/a><\/td>\n<td>WB; IHC; ICC; IP.<\/td>\n<\/tr>\n<tr>\n<td>PAA037Hu02<\/td>\n<td><a href=\"http:\/\/www.cloud-clone.com\/products\/PAA037Hu02.html\">Polyclonal Antibody to Fibronectin (FN)<\/a><\/td>\n<td>WB; IHC; ICC; IP.<\/td>\n<\/tr>\n<tr>\n<td>PAA037Hu03<\/td>\n<td><a href=\"http:\/\/www.cloud-clone.com\/products\/PAA037Hu03.html\">Polyclonal Antibody to Fibronectin (FN)<\/a><\/td>\n<td>WB; IHC; ICC; IP.<\/td>\n<\/tr>\n<tr>\n<td>LAA037Hu71<\/td>\n<td><a href=\"http:\/\/www.cloud-clone.com\/products\/LAA037Hu71.html\">Biotin-Linked Polyclonal Antibody to Fibronectin (FN)<\/a><\/td>\n<td>WB; IHC; ICC.<\/td>\n<\/tr>\n<tr>\n<td>MAA037Hu22<\/td>\n<td><a href=\"http:\/\/www.cloud-clone.com\/products\/MAA037Hu22.html\">Monoclonal Antibody to Fibronectin (FN)<\/a><\/td>\n<td>WB; IHC; ICC; IP.<\/td>\n<\/tr>\n<tr>\n<td>SEA037Hu<\/td>\n<td><a href=\"http:\/\/www.cloud-clone.com\/products\/SEA037Hu.html\">ELISA Kit for Fibronectin (FN)<\/a><\/td>\n<td>Enzyme-linked immunosorbent assay for Antigen Detection.<\/td>\n<\/tr>\n<tr>\n<td>HEA037Hu<\/td>\n<td><a href=\"http:\/\/www.cloud-clone.com\/products\/HEA037Hu.html\">High Sensitive ELISA Kit for Fibronectin (FN)<\/a><\/td>\n<td>Enzyme-linked immunosorbent assay for Antigen Detection.<\/td>\n<\/tr>\n<tr>\n<td>SCA037Hu<\/td>\n<td><a href=\"http:\/\/www.cloud-clone.com\/products\/SCA037Hu.html\">CLIA Kit for Fibronectin (FN)<\/a><\/td>\n<td>Chemiluminescent immunoassay for Antigen Detection.<\/td>\n<\/tr>\n<tr>\n<td>LMA037Hu<\/td>\n<td><a href=\"http:\/\/www.cloud-clone.com\/products\/LMA037Hu.html\">Magnetic Luminex Assay Kit for Fibronectin (FN) ,etc.<\/a><\/td>\n<td>Magnetic Luminex Assay for Antigen Detection.<\/td>\n<\/tr>\n<tr>\n<td>KSA037Hu01<\/td>\n<td><a href=\"http:\/\/www.cloud-clone.com\/products\/KSA037Hu01.html\">ELISA Kit DIY Materials for Fibronectin (FN)<\/a><\/td>\n<td>Main materials for &#8220;Do It (ELISA Kit) Yourself&#8221;.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Fibronectin (FN)\u6d3b\u6027\u91cd\u7d44\u86cb\u767d | Active Fibronectin (FN)\u8ca8\u865fAPA03 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":38092,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[5234],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/38094"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=38094"}],"version-history":[{"count":8,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/38094\/revisions"}],"predecessor-version":[{"id":49607,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/38094\/revisions\/49607"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/38092"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=38094"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=38094"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=38094"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}