{"id":38071,"date":"2023-02-07T16:55:12","date_gmt":"2023-02-07T08:55:12","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=38071"},"modified":"2025-03-21T14:21:20","modified_gmt":"2025-03-21T06:21:20","slug":"%e4%ba%ba%e6%af%9b%e5%9b%8a%e7%9c%9f%e7%9a%ae%e4%b9%b3%e9%a0%ad%e7%b4%b0%e8%83%9e-primary-cell-human-hair-follicle-dermal-papilla-cells%e8%b2%a8%e8%99%9ft4059","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=38071","title":{"rendered":"\u4eba\u6bdb\u56ca\u771f\u76ae\u4e73\u982d\u7d30\u80de (Primary cell) | Human Hair Follicle Dermal Papilla cells\u8ca8\u865fT4059"},"content":{"rendered":"

\u4eba\u6bdb\u56ca\u771f\u76ae\u4e73\u982d\u7d30\u80de (Primary cell) | Human Hair Follicle Dermal Papilla cells\u8ca8\u865fT4059 \u00a05×105<\/sup>\u00a0cells \/ 1.0 ml<\/a><\/span><\/strong><\/span><\/p>\n

\u521d\u4ee3\u7d30\u80de\u9700\u914d\u5408TM4059\u57f9\u990a\u57fa\u4f7f\u7528<\/p>\n

\u4eba\u6bdb\u56ca\u771f\u76ae\u4e73\u982d\u7d30\u80deMultipolar | \u4f86\u6e90Hair Follicle | \u5efa\u8b70\u57f9\u990a\u5bc6\u5ea66,000 – 10,000 cells per cm^2 \u3002\u7d30\u80de\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (\u8ca8\u865fG299<\/a>)\uff0c\u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (\u8ca8\u865fG422<\/a>)\u3002Grow cells in ECM-coated culture vessels with the following conditions. \u57f9\u990a\u6db2Prigrow X series (TM4059<\/a>)\u30021%. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0\u00b0C<\/p>\n

\"\"<\/p>\n

\u521d\u4ee3\u7d30\u80de (Primary Cell) \u662f\u6307\u76f4\u63a5\u5f9e\u6d3b\u9ad4\u7d44\u7e54\u4e2d\u5206\u96e2\u51fa\u7684\u7d30\u80de\uff0c\u5177\u6709\u6709\u9650\u7684\u7d30\u80de\u58fd\u547d\u7121\u6cd5\u7121\u9650\u5236\u5730\u7e7c\u4ee3\u57f9\u990a\uff1b\u8207\u4e00\u822c\u5b78\u8853\u4e0a\u5e38\u7528\u7684\u7d30\u80de\u682a (Cell Line)\u4e0d\u540c\uff0c\u5177\u6709\u6709\u9650\u7684\u7d30\u80de\u58fd\u547d\u7121\u6cd5\u7121\u9650\u5236\u5730\u7e7c\u4ee3\u57f9\u990a\uff0c\u4f46\u537b\u5177\u6709\u6700\u63a5\u8fd1\u6d3b\u9ad4\u72c0\u614b\u7684\u7d30\u80de\u578b\u614b\u8207\u529f\u80fd\u7279\u6027\u3002\u4eba\u6bdb\u56ca\u771f\u76ae\u4e73\u982d\u7d30\u80de(Human Hair Follicle Dermal Papilla cells, \u8ca8\u865f T4059)\u662f\u5f9e\u5d4c\u5165\u982d\u76ae\u6bdb\u56ca\u7d30\u80de\u5916\u57fa\u8cea\u7684\u6bdb\u4e73\u982d\u4e2d\u5206\u96e2\u51fa\u4f86\u7684\u9ad8\u6d3b\u6027\u9593\u5145\u8cea\u7d30\u80de(mesenchymal cells)\u3002\u771f\u76ae\u4e73\u982d\u7d30\u80de(Dermal Papilla Cells)\u901a\u904e\u53c3\u8207\u6bdb\u56ca\u7d30\u80de\u7684\u4e0a\u76ae-\u9593\u8cea\u76f8\u4e92\u4f5c\u7528(epithelial-mesenchymal interaction)\uff0c\u5728\u63a7\u5236\u6bdb\u9aee\u751f\u9577\u5468\u671f\u548c\u7522\u751f\u65b9\u9762\u767c\u63ee\u91cd\u8981\u4f5c\u7528\u3002\u5b83\u5011\u7684\u5b58\u6d3b\u53d7\u4fe1\u865f\u8f49\u5c0e\u901a\u8def\u5982 ERK \u548c Akt \u7684\u8abf\u7bc0\u3002\u9019\u4e9b\u7d30\u80de\u53ef\u7528\u65bc\u958b\u767c\u548c\u8a55\u4f30\u6bdb\u9aee\u751f\u9577\u7522\u54c1(hair growth products)\u4ee5\u53ca\u9451\u5b9a\u6bdb\u56ca(hair follicle)\u5167\u7684\u7d30\u80de\u7fa4\u3002\u6b64\u5916\uff0c\u7531\u65bc\u5b83\u5011\u5177\u6709\u96c4\u6fc0\u7d20\u53d7\u9ad4(androgen receptors)\uff0c\u53ef\u7528\u65bc\u96c4\u6fc0\u7d20\u963b\u65b7\u5291 (androgen blocking reagents) \u7684\u9ad4\u5916\u7be9\u9078\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Description<\/strong><\/td>\nHuman Hair Follicle Dermal Papilla cells are highly active mesenchymal cells isolated from the hair papilla embedded in extracellular matrix of scalp hair follicles. Dermal Papilla Cells play a significant role in controlling the hair growth cycle and production by involving in the epithelial-mesenchymal interaction of hair follicle cells. Their survival is regulated by signal transduction pathways such as ERK and Akt. These cells can be used for development and evaluation of hair growth products and identifying cell populations within the hair follicle. In addition, they can be used for\u00a0in vitro\u00a0<\/em>screening of androgen blocking reagents since they have androgen receptors.<\/td>\n<\/tr>\n
SKU<\/strong><\/td>\nT4059<\/td>\n<\/tr>\n
Species<\/strong><\/td>\nHuman (H. sapiens)<\/td>\n<\/tr>\n
Tissue\/Organ\/Organ System<\/strong><\/td>\nHair Follicle<\/td>\n<\/tr>\n
Cell Morphology<\/strong><\/td>\nMultipolar<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nFor Research Use Only<\/td>\n<\/tr>\n
Seeding Density<\/strong><\/td>\n6,000 – 10,000 cells per cm^2<\/td>\n<\/tr>\n
Population Doubling Time<\/strong><\/td>\n10 PD. Doubling time: 22.35 hours<\/td>\n<\/tr>\n
Donor Gender<\/strong><\/td>\nFemale<\/td>\n<\/tr>\n
Donor Gender<\/strong><\/td>\nFemale<\/td>\n<\/tr>\n
Donor Age<\/strong><\/td>\n56<\/td>\n<\/tr>\n
Donor Disease<\/strong><\/td>\nNormal<\/td>\n<\/tr>\n
Donor Ethnicity<\/strong><\/td>\nCaucasian<\/td>\n<\/tr>\n
Cell Type<\/strong><\/td>\nPrimary Cells<\/td>\n<\/tr>\n
Growth Properties<\/strong><\/td>\nAdherent<\/td>\n<\/tr>\n
Propagation Requirements<\/strong><\/td>\n\n

Use of PriCoat<\/strong>TM<\/sup><\/strong>T25 Flasks (G299<\/a>) or Applied Cell Extracellular Matrix (G422<\/a>) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.<\/strong><\/p>\n

The base medium for this cell line is Prigrow X series medium available at\u00a0abm<\/strong>, Cat. No.\u00a0TM4059<\/a>.
\nCarbon dioxide (CO2<\/sub>): 5%, Temperature: 37.0<\/td>\n<\/tr>\n

Subculture Protocol<\/strong><\/td>\n0<\/td>\n<\/tr>\n
Unit quantity<\/strong><\/td>\n5×105<\/sup>\u00a0cells \/ 1.0 ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

HOT!!<\/strong>\u00a0<\/u>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a>PriCoat\u2122 T25 Flasks | type I collagen coating | <\/a>I \u578b\u81a0\u539f(type I collagen) | Applied Cell Extracellular Matrix | <\/a>Prigrow III Medium\u521d\u4ee3\u7d30\u80de\u57f9\u990a\u6db2<\/a><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u4eba\u6bdb\u56ca\u771f\u76ae\u4e73\u982d\u7d30\u80de (Primary cell) | Human Hair Follicle Dermal P […]<\/p>\n","protected":false},"author":3,"featured_media":38072,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[5230],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/38071"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=38071"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/38071\/revisions"}],"predecessor-version":[{"id":50786,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/38071\/revisions\/50786"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/38072"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=38071"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=38071"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=38071"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}