{"id":37909,"date":"2023-02-05T10:22:57","date_gmt":"2023-02-05T02:22:57","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=37909"},"modified":"2023-04-18T18:17:57","modified_gmt":"2023-04-18T10:17:57","slug":"%e9%87%8d%e7%b5%84%e6%8a%97-egfr-%e6%8a%97%e9%ab%94-ep38y-recombinant-anti-egfr-antibody-ep38y-abcam%e8%b2%a8%e8%99%9fab52894","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=37909","title":{"rendered":"\u91cd\u7d44\u6297 EGFR \u6297\u9ad4 [EP38Y] | Recombinant Anti-EGFR antibody [EP38Y] Abcam\u8ca8\u865fab52894"},"content":{"rendered":"<p><span style=\"color: #33cccc;\"><a style=\"color: #33cccc;\" href=\"http:\/\/biopioneer.com.tw\/?p=37909\"><strong><span style=\"font-size: 12pt;\">\u91cd\u7d44\u6297 EGFR \u6297\u9ad4 [EP38Y] | Recombinant Anti-EGFR antibody [EP38Y] Abcam\u8ca8\u865fab52894<\/span><\/strong><\/a><\/span><\/p>\n<p><span class=\"pws_link\"><a id=\"ds_header_abreview_link\" class=\"scroll_to\" href=\"https:\/\/www.abcam.com\/egfr-antibody-ep38y-ab52894.html?productWallTab=Abreviews\">Reviews (22)<\/a>\u00a0 \u00a0<\/span><a id=\"ds_header_Questions_link\" class=\"pws_link pws_questions hidden-lt-med scroll_to\" href=\"https:\/\/www.abcam.com\/egfr-antibody-ep38y-ab52894.html?productWallTab=Questions\">Q&amp;A (1)<\/a>\u00a0 \u00a0<a class=\"pws_publications pws_link \" href=\"https:\/\/www.abcam.com\/egfr-antibody-ep38y-ab52894.html#description_references\">References (244)<\/a><\/p>\n<p><img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/01\/ABCAM_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1-300x300.jpg\" alt=\"ABCAM_\u5de5\u4f5c\u5340\u57df 1\" \/><\/p>\n<ul>\n<li>\u5154\u55ae\u514b\u9686\u6297\u9ad4 [EP38Y] to EGFR<\/li>\n<li>\u9069\u7528\u65bc\uff1aWB\u3001IP\u3001IHC-P\u3001ICC\/IF\u3001Flow Cyt\uff08Intra\uff09\u3001ELISA<\/li>\n<li>\u6572\u9664\u9a57\u8b49<\/li>\n<li>\u8207\u53cd\u61c9: Mouse, Rat, Human<\/li>\n<\/ul>\n<p><span style=\"color: #800000; font-size: 12pt;\"><strong>\u514d\u75ab\u7d30\u80de\u5316\u5b78\/\u514d\u75ab\u87a2\u5149 &#8211; \u6297 EGFR \u6297\u9ad4 [EP38Y] (ab52894)<\/strong><\/span><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/02\/%E5%85%8D%E7%96%AB%E7%B4%B0%E8%83%9E%E5%8C%96%E5%AD%B8%E5%85%8D%E7%96%AB%E7%86%92%E5%85%89-%E6%8A%97-EGFR-%E6%8A%97%E9%AB%94-EP38Y-ab52894.jpg\" alt=\"\" width=\"392\" height=\"278\" \/><\/p>\n<p>\u672a\u7d14\u5316\u7684 ab52894 \u5c0d A431\uff08\u4eba\u8868\u76ae\u6a23\u764c\u7d30\u80de\u7cfb\uff09\u7d30\u80de\u9032\u884c\u67d3\u8272\u3002\u5c07\u7d30\u80de\u5728 -20\u00b0C \u7684 100% \u7532\u9187\u4e2d\u56fa\u5b9a 5 \u5206\u9418\uff0c\u7136\u5f8c\u5728 1% BSA \/ 10% \u6b63\u5e38\u5c71\u7f8a\u8840\u6e05 \/ 0.1% PBS-Tween \u4e2d\u7684 0.3M \u7518\u6c28\u9178\u4e2d\u5ba4\u6eab\u5b75\u80b2 1 \u5c0f\u6642\u4ee5\u900f\u5316\u7d30\u80de\u548c\u963b\u65b7\u975e\u7279\u7570\u6027\u86cb\u767d\u8cea-\u86cb\u767d\u8cea\u76f8\u4e92\u4f5c\u7528\u3002\u7136\u5f8c\u5c07\u7d30\u80de\u8207\u6297\u9ad4\uff08ab52894\uff0c500 \u5206\u4e4b\u4e00\uff09\u5728 +4\u00b0C \u4e0b\u5b75\u80b2\u904e\u591c\u3002\u4e8c\u6297\uff08\u507d\u7da0\u8272\uff09\u662f\u5c71\u7f8a\u6297\u5154 IgG H&amp;L (Alexa Fluor\u00a0<sup>\u00ae<\/sup>\u00a0488) \u9810\u5438\u9644 (\u00a0<a href=\"https:\/\/www.abcam.com\/ab150081.html\">ab150081<\/a>\u00a0)\uff0c\u5728\u5ba4\u6eab\u4e0b\u4ee5 1\/1000 \u7a00\u91cb\u4f7f\u7528 1 \u5c0f\u6642\u3002Alexa Fluor\u00a0<sup>\u00ae<\/sup>\u00a0594 WGA \u7528\u65bc\u5728\u5ba4\u6eab\u4e0b\u4ee5 1\/200 \u7a00\u91cb 1 \u5c0f\u6642\u4f86\u6a19\u8a18\u8cea\u819c\uff08\u507d\u7d05\u8272\uff09\u3002DAPI \u7528\u65bc\u5728\u5ba4\u6eab\u4e0b\u4ee5 1.43 \u00b5M \u7684\u6fc3\u5ea6\u5c0d\u7d30\u80de\u6838\uff08\u507d\u85cd\u8272\uff09\u67d3\u8272 1 \u5c0f\u6642\u3002<\/p>\n<div class=\"legend__title\"><strong><span style=\"font-size: 12pt; color: #800000;\">\u514d\u75ab\u7d44\u7e54\u5316\u5b78\uff08\u798f\u723e\u99ac\u6797\/PFA \u56fa\u5b9a\u77f3\u881f\u5305\u57cb\u5207\u7247\uff09- \u6297 EGFR \u6297\u9ad4 [EP38Y] (ab52894)<\/span><\/strong><\/div>\n<div class=\"legend__description\">\n<p>\u4f7f\u7528\u7d14\u5316\u7684 ab52894 \u4ee5 1:100 \u7a00\u91cb\u5ea6 (0.95 \u03bcg\/ml) \u6a19\u8a18 EGFR \u7684\u4eba\u5bae\u9838\u764c\u7d44\u7e54\u5207\u7247\u7684\u514d\u75ab\u7d44\u7e54\u5316\u5b78\uff08\u798f\u723e\u99ac\u6797\/PFA \u56fa\u5b9a\u77f3\u881f\u5305\u57cb\u5207\u7247\uff09\u5206\u6790\u3002\u4f7f\u7528 pH 9.0 \u7684 EDTA \u7de9\u885d\u6db2\u9032\u884c\u71b1\u4ecb\u5c0e\u7684\u6297\u539f\u4fee\u5fa9\u3002\u7d44\u7e54\u7528\u8607\u6728\u7cbe\u8907\u67d3\u3002<a href=\"https:\/\/www.abcam.com\/ab97051.html\">ab97051<\/a>\u5c71\u7f8a\u6297\u5154 IgG H&amp;L (HRP) \u4e8c\u6297\u6309 1:500 \u7a00\u91cb\u4f7f\u7528\u3002PBS \u4ee3\u66ff\u4e00\u6297\u4f5c\u70ba\u9670\u6027\u5c0d\u7167\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/02\/%E5%85%8D%E7%96%AB%E7%B5%84%E7%B9%94%E5%8C%96%E5%AD%B8%EF%BC%88%E7%A6%8F%E7%88%BE%E9%A6%AC%E6%9E%97PFA-%E5%9B%BA%E5%AE%9A%E7%9F%B3%E8%A0%9F%E5%8C%85%E5%9F%8B%E5%88%87%E7%89%87%EF%BC%89-%E6%8A%97-EGFR-%E6%8A%97%E9%AB%94-EP38Y-ab52894.jpg\" alt=\"\" width=\"408\" height=\"408\" \/><\/p>\n<div class=\"legend__title\"><span style=\"font-size: 12pt; color: #800000;\"><strong>\u514d\u75ab\u7d30\u80de\u5316\u5b78\/\u514d\u75ab\u87a2\u5149 &#8211; \u6297 EGFR \u6297\u9ad4 [EP38Y] (ab52894)<\/strong><\/span><\/div>\n<div class=\"legend__description\">\n<p>\u4f7f\u7528 1:250 \u7a00\u91cb\u5ea6 (0.4 \u03bcg\/ml) \u7d14\u5316\u7684 ab52894 \u6a19\u8a18 EGFR \u7684 A431\uff08\u4eba\u8868\u76ae\u6a23\u764c\u4e0a\u76ae\u7d30\u80de\uff09\u7d30\u80de\u7684\u514d\u75ab\u7d30\u80de\u5316\u5b78\/\u514d\u75ab\u7192\u5149\u5206\u6790\u3002<\/p>\n<p>\u7d30\u80de\u56fa\u5b9a\u5728 4% \u591a\u805a\u7532\u919b\u4e2d\u4e26\u7528 0.1% Triton X-100 \u900f\u5316\u3002\u7528<a href=\"https:\/\/www.abcam.com\/ab195889.html\">ab195889<\/a>\u6297\u03b1\u5fae\u7ba1\u86cb\u767d\u6297\u9ad4 [DM1A] &#8211; \u5fae\u7ba1\u6a19\u8a18\u7269 (Alexa Fluor\u00a0<sup>\u00ae<\/sup>\u00a0594) 1:200 (2.5 \u03bcg\/ml) \u5c0d\u7d30\u80de\u9032\u884c\u8907\u67d3\u3002<a href=\"https:\/\/www.abcam.com\/ab150077.html\">ab150077<\/a>\u5c71\u7f8a\u6297\u5154 IgG (Alexa Fluor\u00a0<sup>\u00ae<\/sup>\u00a0488) \u4ee5 1:1000 \u7a00\u91cb\u7528\u4f5c\u4e8c\u6297\u3002DAPI \u6838\u8907\u67d3\u3002<\/p>\n<p>PBS \u4ee3\u66ff\u4e00\u6297\u7528\u4f5c\u50c5\u4e8c\u6297\u7684\u5c0d\u7167\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/02\/%E5%85%8D%E7%96%AB%E7%B4%B0%E8%83%9E%E5%8C%96%E5%AD%B8%E5%85%8D%E7%96%AB%E7%86%92%E5%85%89-%E6%8A%97-EGFR-%E6%8A%97%E9%AB%94-EP38Y-ab528941.jpg\" alt=\"\" width=\"397\" height=\"397\" \/><\/p>\n<\/div>\n<\/div>\n<p><a href=\" http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/02\/\u91cd\u7d44\u6297-EGFR-\u6297\u9ad4-EP38Y-Recombinant-Anti-EGFR-antibody-EP38Y-Abcam\u8ca8\u865fab52894.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/01\/%E7%B6%B2%E7%AB%99%E8%A6%8F%E6%A0%BC%E5%B0%8F%E5%9C%96_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-11-300x97.jpg\" alt=\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df 1_\u5de5\u4f5c\u5340\u57df 1\" width=\"149\" height=\"48\" \/><\/a><\/p>\n<h3><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\">\u7522\u54c1\u540d\u7a31<\/span><\/strong><\/span><\/h3>\n<p>\u6297 EGFR \u6297\u9ad4 [EP38Y]<a class=\"see-all-link\" href=\"https:\/\/www.abcam.com\/products?keywords=EGFR&amp;selected.productType=Primary+antibodies&amp;selected.targetName=EGFR\">\u67e5\u770b\u6240\u6709 EGFR \u4e00\u6297<\/a><\/p>\n<h3 style=\"font-style: inherit;\"><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\">\u63cf\u8ff0<\/span><\/strong><\/span><\/h3>\n<p>\u5154\u55ae\u514b\u9686\u6297\u9ad4 [EP38Y] to EGFR<\/p>\n<h3 style=\"font-style: inherit;\"><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\">\u5bbf\u4e3b\u7269\u7a2e<\/span><\/strong><\/span><\/h3>\n<p>\u5154\u5b50<\/p>\n<h3 style=\"font-style: inherit;\"><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\">\u7279\u7570\u6027<\/span><\/strong><\/span><\/h3>\n<p>\u8a72\u7522\u54c1\u7684\u514d\u75ab\u539f\u662f\u4e00\u7a2e\u5408\u6210\u7684\u78f7\u9178\u80bd\uff0c\u5c0d\u61c9\u65bc\u4eba EGFR Tyr1068 \u5468\u570d\u7684\u6b98\u57fa\u3002\u7be9\u9078\u5f8c\uff0c\u767c\u73fe\u514b\u9686\u201cEP38Y\u201d\u53ef\u8b58\u5225\u7e3d EGFR\uff0c\u4e26\u4e14\u5c0d\u78f7\u9178\u5316 Tyr1068 EGFR \u4e0d\u5177\u6709\u7279\u7570\u6027\u3002\u8a72\u7522\u54c1\u5728\u4f7f\u7528\u81ea\u7136\u904e\u5ea6\u8868\u9054 EGFR \u86cb\u767d\u7684 A431\uff08\u4eba\u985e\u9c57\u72c0\u7d30\u80de\u764c\uff09\u88c2\u89e3\u7269\u7684\u86cb\u767d\u8cea\u5370\u8de1\u4e2d\u7522\u751f\u4e86\u5f37\u70c8\u7684\u4fe1\u865f\u3002\u53ef\u80fd\u9700\u8981\u91dd\u5c0d\u9336\u9054\u8f03\u4f4e\u6c34\u5e73\u5167\u6e90\u6027 EGFR \u7684\u7d30\u80de\u7cfb\u548c\u7d44\u7e54\u512a\u5316\u86cb\u767d\u8cea\u5370\u8de1\u689d\u4ef6\u3002\u5c0f\u9f20\u548c\u5927\u9f20\u7684\u63a8\u85a6\u662f\u57fa\u65bc WB \u7d50\u679c\u3002<strong>\u8a72\u6297\u9ad4\u53ef\u80fd\u4e0d\u9069\u7528\u65bc\u5c0f\u9f20\u6216\u5927\u9f20\u6a23\u672c\u7684 IHC\u3002<\/strong><\/p>\n<h3 style=\"font-style: inherit;\"><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\">\u6e2c\u8a66\u61c9\u7528<\/span><\/strong><\/span><\/h3>\n<p><abbr title=\"\u514d\u75ab\u5370\u8de1\">WB<\/abbr>\u00a0,\u00a0<abbr title=\"\u514d\u75ab\u6c89\u6fb1\">IP<\/abbr>\u00a0,\u00a0<abbr title=\"\u514d\u75ab\u7d44\u5316\uff08\u798f\u723e\u99ac\u6797\/PFA \u56fa\u5b9a\u77f3\u881f\u5305\u57cb\u5207\u7247\uff09\">IHC-P<\/abbr>\u00a0,\u00a0<abbr title=\"\u514d\u75ab\u7d30\u80de\u5316\u5b78\/\u514d\u75ab\u7192\u5149\">ICC\/IF<\/abbr>\u00a0,\u00a0<abbr title=\"\u6d41\u5f0f\u7d30\u80de\u8853\uff08\u7d30\u80de\u5167\uff09\">Flow Cyt (Intra)<\/abbr>\u00a0,\u00a0<abbr title=\"\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\">ELISA<\/abbr><\/p>\n<h3 style=\"font-style: inherit;\"><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\">\u7269\u7a2e\u53cd\u61c9\u6027<\/span><\/strong><\/span><\/h3>\n<p><strong>\u8207\u53cd\u61c9:\u00a0<\/strong>Mouse, Rat, Human<\/p>\n<h3 style=\"font-style: inherit;\"><strong><span style=\"font-size: 12pt; color: #800000;\">\u514d\u75ab\u539f<\/span><\/strong><\/h3>\n<p style=\"font-style: inherit; font-weight: inherit;\">\u5408\u6210\u80bd\u3002\u6b64\u4fe1\u606f\u70ba Abcam \u548c\/\u6216\u5176\u4f9b\u61c9\u5546\u5c08\u6709\u3002<br \/>\n\uff08\u80bd\u53ef\u4f5c\u70ba<a href=\"https:\/\/www.abcam.com\/ab204282.html\">ab204282<\/a>\u00a0)<\/p>\n<h3 style=\"font-style: inherit;\"><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\">\u967d\u6027\u5c0d\u7167<\/span><\/strong><\/span><\/h3>\n<p>ICC\/IF\uff1aA431 \u7d30\u80de\u3002WB\uff1aHeLa\u3001Caco-2 \u548c A431 \u7d30\u80de\u88c2\u89e3\u7269\uff1b\u5927\u9f20\u809d\u81df\u548c\u76ae\u819a\u88c2\u89e3\u7269\uff1b\u5c0f\u9f20\u80ba\u548c\u76ae\u819a\u88c2\u89e3\u7269 IP\uff1aHeLa \u5168\u7d30\u80de\u88c2\u89e3\u7269 (\u00a0<a href=\"https:\/\/www.abcam.com\/ab150035.html\">ab150035<\/a>\u00a0)\u3002Flow Cyt\uff08\u5167\u90e8\uff09\uff1aA431 \u7d30\u80de\u3002IHC-P\uff1a\u4eba\u5bae\u9838\u764c<\/p>\n<p><strong><span style=\"font-size: 12pt;\"><a style=\"color: #ff6600;\" href=\"http:\/\/biopioneer.com.tw\/?p=37883\">\u6297 BRAF\uff08\u7a81\u8b8a\u7684 V600E\uff09\u6297\u9ad4<\/a>\u00a0|\u00a0<a style=\"color: #ff6600;\" href=\"http:\/\/biopioneer.com.tw\/?p=37904\">\u6297 HMGB1 \u6297\u9ad4<\/a>\u00a0|\u00a0<a style=\"color: #ff6600;\" href=\"http:\/\/biopioneer.com.tw\/?p=37909\">\u6297 EGFR \u6297\u9ad4 [EP38Y]\u00a0<\/a>\u00a0|\u00a0<a style=\"color: #ff6600;\" href=\"http:\/\/biopioneer.com.tw\/?p=37909\">\u6297 NF-kB p65 \u6297\u9ad4\u00a0<\/a>\u00a0|\u00a0<a style=\"color: #ff6600;\" href=\"http:\/\/biopioneer.com.tw\/?p=37915\">\u6297 Ki67 \u6297\u9ad4<\/a>\u00a0|\u00a0<a style=\"color: #ff6600;\" href=\"http:\/\/biopioneer.com.tw\/?p=37919\">IV \u578b\u81a0\u539f\u86cb\u767d\u6297\u9ad4<\/a><\/span><\/strong><\/p>\n<p><a href=\"https:\/\/www.abcam.com\/\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/01\/%E7%B6%B2%E7%AB%99%E8%A6%8F%E6%A0%BC%E5%B0%8F%E5%9C%96-%E5%93%81%E7%89%8C%E4%BB%8B%E7%B4%B9_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1-300x120.jpg\" alt=\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716 \u54c1\u724c\u4ecb\u7d39_\u5de5\u4f5c\u5340\u57df 1\" width=\"146\" height=\"60\" \/><\/a><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/02\/abcam.png\" alt=\"\" width=\"166\" height=\"50\" \/><\/p>\n<p>http:\/\/www.abcam.com\/<\/p>\n<p>Abcam\u8b58\u5225\u3001\u958b\u767c\u548c\u63d0\u4f9b\u9ad8\u8cea\u91cf\u7684\u751f\u7269\u8a66\u5291\u548c\u5de5\u5177\uff0c\u5b83\u5011\u5728\u5305\u62ec\u85e5\u7269\u767c\u73fe\u3001\u8a3a\u65b7\u548c\u57fa\u790e\u7814\u7a76\u5728\u5167\u7684\u5ee3\u6cdb\u9818\u57df\u548c\u61c9\u7528\u4e2d\u5fc5\u4e0d\u53ef\u5c11\u3002Abcam \u5c08\u6ce8\u5404\u7a2e\u7814\u7a76\u3001\u8a3a\u65b7\u53ca\u6cbb\u7642\u7528\u4e4b\u55ae\u682a\u6297\u9ad4\u7684\u958b\u767c\uff0c\u64c1\u6709 RabMAb\u00ae \u5154\u5b50\u55ae\u682a\u6297\u9ad4\u751f\u7522\u6280\u8853\u5c08\u5229\u4e4b\u539f\u59cb\u5ee0\u5546\uff0c\u73fe\u4eca\u70ba Abcam 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