{"id":37883,"date":"2023-02-04T23:18:40","date_gmt":"2023-02-04T15:18:40","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=37883"},"modified":"2023-04-18T18:18:33","modified_gmt":"2023-04-18T10:18:33","slug":"%e9%87%8d%e7%b5%84%e6%8a%97-braf%ef%bc%88%e7%aa%81%e8%ae%8a%e7%9a%84-v600e%ef%bc%89%e6%8a%97%e9%ab%94-recombinant-anti-braf-mutated-v600e-antibody-ve1-abcam%e8%b2%a8%e8%99%9fab228461","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=37883","title":{"rendered":"\u91cd\u7d44\u6297 BRAF\uff08\u7a81\u8b8a\u7684 V600E\uff09\u6297\u9ad4 | Recombinant Anti-BRAF (mutated V600E) antibody [VE1]-Abcam\u8ca8\u865fab228461"},"content":{"rendered":"

\u91cd\u7d44\u6297 BRAF\uff08\u7a81\u8b8a\u7684 V600E\uff09\u6297\u9ad4 | Recombinant Anti-BRAF (mutated V600E) antibody [VE1] Abcam\u8ca8\u865fab228461<\/a><\/span><\/strong><\/span><\/p>\n

Reviews\u00a0<\/strong>(<\/strong>6<\/strong><\/span>)<\/strong><\/a>\u00a0\u00a0Submit a question<\/strong><\/a>\u00a0\u00a0References (100)<\/strong><\/a><\/span><\/p>\n

\"ABCAM_\u5de5\u4f5c\u5340\u57df<\/p>\n

\u5c0f\u9f20\u55ae\u514b\u9686\u6297\u9ad4 [VE1] \u81f3 BRAF\uff08\u7a81\u8b8a\u7684 V600E\uff09\uff0c\u9069\u7528\u65bc\uff1aIHC-P\u3001WB\uff0c\u53cd\u61c9\u7269\u7a2eHuman\u3002<\/span><\/strong><\/p>\n

\"\"<\/p>\n

\u7528 ab228461 \u4ee5 1\/100 \u7a00\u91cb\u5ea6\u6a19\u8a18 BRAF\uff08\u7a81\u8b8a\u7684 V600E\uff09\uff0c\u5c0d\u798f\u723e\u99ac\u6797\u56fa\u5b9a\u77f3\u881f\u5305\u57cb\u7684\u4eba\u9ed1\u8272\u7d20\u7624\u7d44\u7e54\uff08\u651c\u5e36\u7a81\u8b8a\u9ad4 BRAF V600E\uff09\u9032\u884c\u514d\u75ab\u7d44\u7e54\u5316\u5b78\u5206\u6790\u3002\u4f7f\u7528\u6ab8\u6aac\u9178\u9209\u7de9\u885d\u6db2\uff08pH6\uff0c\u8868\u4f4d\u4fee\u5fa9\u6eb6\u6db2 1\uff09\u4f7f\u7528\u71b1\u4ecb\u5c0e\u7684\u6297\u539f\u4fee\u5fa9\u65b9\u6cd5\u5c0d\u5207\u7247\u9032\u884c 20 \u5206\u9418\u7684\u9810\u8655\u7406\u3002\u7136\u5f8c\u5c07\u5207\u7247\u8207 1\/100 \u7a00\u91cb\u7684 ab228461 \u5728\u5ba4\u6eab\u4e0b\u5b75\u80b2\uff0c\u4e26\u4f7f\u7528 Leica BOND\u00a0\u2122<\/strong>\u00a0Polymer \u7cbe\u88fd\u8a66\u5291\u76d2\u9032\u884c\u6aa2\u6e2c\u3002<\/p>\n

\"\"<\/p>\n

\u86cb\u767d\u8cea\u5370\u8de1 – \u6297 BRAF\uff08\u7a81\u8b8a\u7684 V600E\uff09\u6297\u9ad4 [VE1] (ab228461)<\/strong><\/span><\/div>\n
\n

\u6240\u6709\u6cf3\u9053\uff1a<\/b>\u00a01\/1000 \u7a00\u91cb\u5ea6\u7684\u6297 BRAF\uff08\u7a81\u8b8a V600E\uff09\u6297\u9ad4 [VE1] (ab228461)<\/p>\n

\u6cf3\u9053 1\uff1a<\/b>\u00a0HCT 116 \u7d30\u80de\u88c2\u89e3\u7269\uff08\u91ce\u751f\u578b BRAF\uff09
\n\u6cf3\u9053 2\uff1a<\/b>\u00a0SW480 \u7d30\u80de\u88c2\u89e3\u7269\uff08\u91ce\u751f\u578b BRAF\uff09
\n\u6cf3\u9053 3\uff1a<\/b>\u00a0Caco-2 \u7d30\u80de\u88c2\u89e3\u7269\uff08\u91ce\u751f\u578b BRAF\uff09
\n\u7b2c 4 \u9053\uff1a<\/b>\u00a0HT-29 \u7d30\u80de\u88c2\u89e3\u7269\uff08\u7a81\u8b8a\u9ad4 BRAF V600E\uff09
\n\u7b2c 5 \u9053\uff1a<\/b>\u00a0A375 \u7d30\u80de\u88c2\u89e3\u7269\uff08\u7a81\u8b8a\u9ad4 BRAF V600E\uff09<\/p>\n

\"\"<\/p>\n<\/div>\n

\n
\u514d\u75ab\u7d44\u7e54\u5316\u5b78\uff08\u798f\u723e\u99ac\u6797\/PFA \u56fa\u5b9a\u77f3\u881f\u5305\u57cb\u5207\u7247\uff09- \u6297 BRAF\uff08\u7a81\u8b8a V600E\uff09\u6297\u9ad4 [VE1] (ab228461)<\/strong><\/span><\/div>\n
\n

\u4f7f\u7528\u6a19\u6e96\u65b9\u6848\u5728 Leica BONDTM \u7cfb\u7d71\u4e0a\u57f7\u884c\u7684\u798f\u723e\u99ac\u6797\u56fa\u5b9a\u77f3\u881f\u5305\u57cb A375 \u7d30\u80de\u7cfb\uff08\u651c\u5e36\u7a81\u8b8a BRAF V600E\uff09\u5207\u7247\u4e2d BRAF\uff08\u7a81\u8b8a V600E\uff09\u67d3\u8272\u7684 IHC \u5716\u50cf\u3002\u4f7f\u7528\u6ab8\u6aac\u9178\u9209\u7de9\u885d\u6db2\uff08pH6\uff0c\u8868\u4f4d\u4fee\u5fa9\u6eb6\u6db2 1\uff09\u4f7f\u7528\u71b1\u4ecb\u5c0e\u7684\u6297\u539f\u4fee\u5fa9\u5c0d\u5207\u7247\u9032\u884c 20 \u5206\u9418\u7684\u9810\u8655\u7406\u3002\u7136\u5f8c\u5c07\u5207\u7247\u8207 5ug\/ml ab228461 \u5728\u5ba4\u6eab\u4e0b\u5b75\u80b2 15 \u5206\u9418\uff0c\u4e26\u4f7f\u7528 HRP \u5171\u8edb\u7dfb\u5bc6\u805a\u5408\u7269\u7cfb\u7d71\u9032\u884c\u6aa2\u6e2c\u3002DAB \u7528\u4f5c\u8272\u539f\u9ad4\u3002\u7136\u5f8c\u5c07\u5207\u7247\u7528\u8607\u6728\u7cbe\u8907\u67d3\u4e26\u7528 DPX \u5c01\u7247\u3002\u63d2\u5716\u50c5\u6b21\u8981\u63a7\u88fd\u5716\u50cf\u53d6\u81ea\u6c92\u6709\u521d\u7d1a\u6297\u9ad4\u7684\u76f8\u540c\u6e2c\u5b9a\u3002<\/p>\n

\u8a72\u5716\u50cf\u662f\u4f7f\u7528\u524d\u4e00\u6279\u5728\u5167\u90e8\u751f\u6210\u7684\uff0c\u4f7f\u7528\u96dc\u4ea4\u7624\u751f\u7522\u65b9\u6cd5\u88fd\u9020\u3002<\/p>\n

\"\"<\/p>\n

\u514d\u75ab\u7d44\u7e54\u5316\u5b78\uff08\u798f\u723e\u99ac\u6797\/PFA \u56fa\u5b9a\u77f3\u881f\u5305\u57cb\u5207\u7247\uff09- \u6297 BRAF\uff08\u7a81\u8b8a V600E\uff09\u6297\u9ad4 [VE1] (ab228461)<\/strong><\/span><\/div>\n
\n

\u9670\u6027\u5c0d\u7167\u7d30\u80de\u7cfb\u5716\u50cf\uff1a\u4f7f\u7528\u6a19\u6e96\u65b9\u6848\u5728 Leica BONDTM \u7cfb\u7d71\u4e0a\u57f7\u884c\u7684\u798f\u723e\u99ac\u6797\u56fa\u5b9a\u77f3\u881f\u5305\u57cb Caco-2 \u7d30\u80de\u7cfb\u5207\u7247\u4e2d BRAF\uff08\u7a81\u8b8a V600E\uff09\u67d3\u8272\u7684 IHC \u5716\u50cf\u3002\u4f7f\u7528\u6ab8\u6aac\u9178\u9209\u7de9\u885d\u6db2\uff08pH6\uff0c\u8868\u4f4d\u4fee\u5fa9\u6eb6\u6db2 1\uff09\u4f7f\u7528\u71b1\u4ecb\u5c0e\u7684\u6297\u539f\u4fee\u5fa9\u5c0d\u5207\u7247\u9032\u884c 20 \u5206\u9418\u7684\u9810\u8655\u7406\u3002\u7136\u5f8c\u5c07\u5207\u7247\u8207 5ug\/ml ab228461 \u5728\u5ba4\u6eab\u4e0b\u5b75\u80b2 15 \u5206\u9418\uff0c\u4e26\u4f7f\u7528 HRP \u5171\u8edb\u7dfb\u5bc6\u805a\u5408\u7269\u7cfb\u7d71\u9032\u884c\u6aa2\u6e2c\u3002DAB \u7528\u4f5c\u8272\u539f\u9ad4\u3002\u7136\u5f8c\u5c07\u5207\u7247\u7528\u8607\u6728\u7cbe\u8907\u67d3\u4e26\u7528 DPX \u5c01\u7247\u3002\u63d2\u5716\u50c5\u6b21\u8981\u63a7\u88fd\u5716\u50cf\u53d6\u81ea\u6c92\u6709\u521d\u7d1a\u6297\u9ad4\u7684\u76f8\u540c\u6e2c\u5b9a\u3002<\/p>\n

\u8a72\u5716\u50cf\u662f\u4f7f\u7528\u524d\u4e00\u6279\u5728\u5167\u90e8\u751f\u6210\u7684\uff0c\u4f7f\u7528\u96dc\u4ea4\u7624\u751f\u7522\u65b9\u6cd5\u88fd\u9020\u3002<\/p>\n<\/div>\n<\/div>\n<\/div>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

\u7522\u54c1\u540d\u7a31<\/span><\/strong><\/span><\/h3>\n

\u6297 BRAF\uff08\u7a81\u8b8a\u7684 V600E\uff09\u6297\u9ad4 [VE1]\u00a0\u67e5\u770b\u6240\u6709<\/strong>\u00a0BRAF\u00a0<\/strong>\u4e00\u6297<\/strong><\/a><\/span><\/p>\n

\u63cf\u8ff0<\/span><\/strong><\/span><\/h3>\n

\u5c0f\u9f20\u55ae\u514b\u9686\u6297\u9ad4 [VE1] \u81f3 BRAF\uff08\u7a81\u8b8a\u7684 V600E\uff09<\/p>\n

\u5bbf\u4e3b\u7269\u7a2e<\/span><\/strong><\/span><\/h3>\n

\u8001\u9f20<\/p>\n

\u7279\u7570\u6027<\/span><\/strong><\/span><\/h3>\n

VE1 \u55ae\u514b\u9686\u6297\u9ad4\u662f\u4e00\u7a2e\u654f\u611f\u6297\u9ad4\uff0c\u53ef\u6aa2\u6e2c\u7a81\u8b8a\u7684\u7d44\u6210\u578b\u6d3b\u6027 BRAF \u86cb\u767d\uff0c\u5176\u4e2d\u5bc6\u78bc\u5b50 600 \u8655\u5b58\u5728\u8c37\u6c28\u9178\u800c\u4e0d\u662f\u7e88\u6c28\u9178 (V600E)\uff08PubMed ID\uff1a21638088\u300123657789\uff09\u3002\u8acb\u6ce8\u610f\uff0c\u8a72\u6297\u9ad4\u5df2\u5831\u544a\u975e\u7279\u7570\u6027\u6838\u67d3\u8272\uff08PubMed ID\uff1a23763264\u300123589031\u300124838325\uff09\u3002<\/p>\n

\u6e2c\u8a66\u61c9\u7528<\/span><\/strong><\/span><\/h3>\n

\u9069\u7528\u65bc\uff1a<\/strong>\u00a0<\/strong>IHC-P\u00a0,\u00a0WB\u66f4\u591a\u8a73\u60c5<\/strong><\/p>\n

\u7269\u7a2e\u53cd\u61c9\u6027<\/span><\/strong><\/span><\/h3>\n

\u53cd\u61c9\u5c0d\u8c61\uff1a<\/strong>\u00a0<\/strong>\u4eba\u985e
\n\u9810\u8a08\u5408\u4f5c\u5c0d\u8c61\uff1a<\/strong>\u00a0<\/strong>\u5c0f\u9f20\u3001\u96de<\/p>\n

\u514d\u75ab\u539f<\/span><\/strong><\/h3>\n

\u5408\u6210\u80bd\u3002\u6b64\u4fe1\u606f\u70ba Abcam \u548c\/\u6216\u5176\u4f9b\u61c9\u5546\u5c08\u6709\u3002<\/p>\n

\u967d\u6027\u5c0d\u7167<\/span><\/strong><\/span><\/h3>\n

WB\uff1aA375 \u7d30\u80de\u88c2\u89e3\u7269\u3002IHC-P\uff1aA375\u3001Caco-2 \u548c\u5177\u6709 B-RAF V600E \u7a81\u8b8a\u7684\u4eba\u9ed1\u8272\u7d20\u7624\u7d44\u7e54\u3002<\/p>\n

\u6297 BRAF\uff08\u7a81\u8b8a\u7684 V600E\uff09\u6297\u9ad4<\/a>\u00a0|\u00a0\u6297 HMGB1 \u6297\u9ad4<\/a>\u00a0|\u00a0\u6297 EGFR \u6297\u9ad4 [EP38Y]\u00a0<\/a>\u00a0|\u00a0\u6297 NF-kB p65 \u6297\u9ad4\u00a0<\/a>\u00a0|\u00a0\u6297 Ki67 \u6297\u9ad4<\/a>\u00a0|\u00a0IV \u578b\u81a0\u539f\u86cb\u767d\u6297\u9ad4<\/a><\/span><\/strong><\/span><\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716<\/a><\/p>\n

\"\"<\/p>\n

http:\/\/www.abcam.com\/<\/p>\n

Abcam\u8b58\u5225\u3001\u958b\u767c\u548c\u63d0\u4f9b\u9ad8\u8cea\u91cf\u7684\u751f\u7269\u8a66\u5291\u548c\u5de5\u5177\uff0c\u5b83\u5011\u5728\u5305\u62ec\u85e5\u7269\u767c\u73fe\u3001\u8a3a\u65b7\u548c\u57fa\u790e\u7814\u7a76\u5728\u5167\u7684\u5ee3\u6cdb\u9818\u57df\u548c\u61c9\u7528\u4e2d\u5fc5\u4e0d\u53ef\u5c11\u3002Abcam \u5c08\u6ce8\u5404\u7a2e\u7814\u7a76\u3001\u8a3a\u65b7\u53ca\u6cbb\u7642\u7528\u4e4b\u55ae\u682a\u6297\u9ad4\u7684\u958b\u767c\uff0c\u64c1\u6709 RabMAb\u00ae \u5154\u5b50\u55ae\u682a\u6297\u9ad4\u751f\u7522\u6280\u8853\u5c08\u5229\u4e4b\u539f\u59cb\u5ee0\u5546\uff0c\u73fe\u4eca\u70ba Abcam \u7684\u5408\u4f5c\u5925\u4f34\u3002\u8207\u5c0f\u9f20\u55ae\u682a\u6297\u9ad4\u76f8\u6bd4\uff0c\u5154\u5b50\u55ae\u682a\u6297\u9ad4\u5177\u6709\u66f4\u512a\u826f\u7684\u6297\u539f\u8fa8\u8b58\u529b\u3001\u66f4\u5f37\u7684\u89aa\u548c\u529b\u8207\u66f4\u9ad8\u7684\u5c08\u4e00\u5ea6\uff0c\u662f\u60a8\u5be6\u9a57\u7528\u55ae\u682a\u6297\u9ad4\u7684\u6700\u4f73\u9078\u64c7\u3002<\/p>\n

Abcam\u70ba\u4e16\u754c\u4e0a\u4e09\u5206\u4e4b\u4e8c\u7684\u751f\u547d\u79d1\u5b78\u5bb6\u63d0\u4f9b\u8d85\u904e 110,000 \u7a2e\u7522\u54c1\uff0c\u7528\u65bc\u7814\u7a76\u86cb\u767d\u8cea\u9776\u6a19 – \u6db5\u84cb\u6297\u9ad4\u3001\u86cb\u767d\u8cea\u3001\u88c2\u89e3\u7269\u3001\u751f\u7269\u5316\u5b78\u88fd\u54c1\u3001\u8a66\u5291\u76d2\u548c\u6aa2\u6e2c\u8a66\u5291\u76d2\u3002 ABCAM\u5728\u5168\u7403\u7684\u5c08\u696d\u8a2d\u65bd\u4e2d\u751f\u7522\u81ea\u5df1\u7684\u7522\u54c1\uff0c\u4e26\u5f9e 400 \u591a\u500b\u63a1\u8cfc\u5546\u8655\u63a1\u8cfc\u5925\u4f34\u3002\u8207\u751f\u547d\u79d1\u5b78\u884c\u696d\u5408\u4f5c\uff0c\u4fc3\u9032\u672a\u4f86\u85e5\u7269\u7684\u958b\u767c\u3002<\/span><\/p>\n

\u7279\u8272\u7522\u54c1\uff1a9\u842c\u591a\u7a2e\u9ad8\u8cea\u91cf\u4e00\u6297<\/a>\u30012800\u591a\u7a2e\u4e8c\u6297<\/a>\u300190\u5206\u9418\u5feb\u901f\u578b\u53ca\u50b3\u7d71ELISA \u548c\u5339\u914d\u7684\u6297\u9ad4\u5c0d<\/a>\u3001\u6572\u9664\u7d30\u80de\u7cfb\u548c\u88c2\u89e3\u7269<\/a>\u3001\u6fc0\u52d5\u5291\u3001\u62ee\u6297\u5291\u3001\u6fc0\u6d3b\u5291\u548c\u6291\u88fd\u5291<\/a>\uff0cDNA\u7532\u57fa\u5316\u8207\u7d44\u86cb\u767d\u4fee\u98fe\u8a66\u5291\uff0c\u7dda\u7c92\u9ad4\u7814\u7a76\u8a66\u5291\uff0c\u7d30\u80de\u6d3b\u6027\u53ca\u51cb\u4ea1\u7b49\u529f\u80fd\u6aa2\u6e2c\u8a66\u5291\u76d2\uff0c\u4eba\u6e90\u5316\u7d30\u80de\u56e0\u5b50\u53ca\u91cd\u7d44\u86cb\u767d\u7b49\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u91cd\u7d44\u6297 BRAF\uff08\u7a81\u8b8a\u7684 V600E\uff09\u6297\u9ad4 | Recombinant Anti-BRAF (mutated […]<\/p>\n","protected":false},"author":3,"featured_media":37626,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5168],"tags":[5206],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37883"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=37883"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37883\/revisions"}],"predecessor-version":[{"id":37921,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37883\/revisions\/37921"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/37626"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=37883"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=37883"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=37883"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}