{"id":37870,"date":"2023-02-04T17:34:32","date_gmt":"2023-02-04T09:34:32","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=37870"},"modified":"2023-10-16T17:38:18","modified_gmt":"2023-10-16T09:38:18","slug":"%e7%a2%98%e5%8c%96%e4%b8%99%e5%95%b6propidium-iodide-pi-%e6%a0%b8%e9%85%b8%e6%9f%93%e5%8a%91-a-red-fluorescent-dna-stain-cas-25535-16-4-apexbio%e8%b2%a8%e8%99%9fb7758","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=37870","title":{"rendered":"\u7898\u5316\u4e19\u5576(Propidium iodide, PI) \u6838\u9178\u67d3\u5291 | A red-fluorescent DNA stain CAS# 25535-16-4 APExBIO\u8ca8\u865fB7758"},"content":{"rendered":"

\u7898\u5316\u4e19\u5576(Propidium iodide, PI) \u6838\u9178\u67d3\u5291 | A red-fluorescent DNA stain CAS# 25535-16-4 APExBIO\u8ca8\u865fB7758<\/strong><\/span><\/p>\n

A red-fluorescent DNA stain<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/span><\/strong><\/span><\/p>\n

\u7898\u5316\u4e19\u5576(Propidium iodide)\u662f\u4e00\u7a2e\u7d30\u80de\u819c\u4e0d\u53ef\u6ef2\u900f(cell-membrane impermeable)\u7684\u67d3\u6599\uff0c\u7d93\u5e38\u7528\u65bc DNA \u67d3\u8272\uff0c\u6700\u5927\u6fc0\u767c\u6ce2\u9577(excitation maximum)\u70ba 535 nm\uff0c\u6700\u5927\u767c\u5c04\u6ce2\u9577(emission maximum)\u70ba 617 nm\u3002\u96d6\u7136\u7898\u5316\u4e19\u5576(Propidium iodide)\u901a\u5e38\u88ab\u6392\u9664\u5728\u5177\u6709\u5b8c\u6574\u819c(intact membranes)\u7684\u6d3b\u7d30\u80de\u4e4b\u5916\uff0c\u4f46\u5b83\u53ef\u4ee5\u8f15\u9b06\u7a7f\u900f\u6b7b\u4ea1(dead)\u6216\u53d7\u640d\u7d30\u80de(damaged cells)\uff0c\u9019\u4f7f\u5f97\u5b83\u901a\u5e38\u7528\u65bc\u9451\u5b9a\u7d30\u80de\u7fa4\u4e2d\u7684\u7d30\u80de\u6d3b\u529b(cell viability)\u6216\u7528\u4f5c\u591a\u8272\u87a2\u5149\u6280\u8853(multicolor fluorescent techniques)\u4e2d\u7684\u8907\u67d3\u5291\u3002\u7898\u5316\u4e19\u5576(Propidium iodide)\u4ee5\u6bcf 4 ~ 5 \u500b\u9e7c\u57fa\u5c0d\u4e00\u7a2e\u67d3\u6599\u7684\u5316\u5b78\u8a08\u91cf\u5d4c\u5165\u6838\u9178\uff0c\u5e7e\u4e4e\u6c92\u6709\u5e8f\u5217\u504f\u597d\uff0c\u7136\u5f8c\u901a\u904e\u87a2\u5149\u986f\u5fae\u93e1(microscopy)\u548c\u6d41\u5f0f\u7d30\u80de\u8853(flow cytometry)\u6aa2\u6e2c\u3002\u7898\u5316\u4e19\u5576(Propidium iodide)\u5df2\u88ab\u8b49\u660e\u662f\u8a55\u4f30\u7570\u8cea\u7d44\u7e54(heterogeneous tissues)\uff08\u5305\u62ec\u9aa8\u9ad3\u3001\u6dcb\u5df4\u7d50\u548c\u80f8\u817a\uff09\u4e2d\u67d0\u4e9b\u7d30\u80de\u7fa4\u51cb\u4ea1(apoptosis)\u7684\u6709\u7528\u5de5\u5177\u3002<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a>\u00a0<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Physical Appearance<\/td>\nA crystalline solid<\/td>\n<\/tr>\n
Storage<\/td>\nStore at -20\u00b0C<\/td>\n<\/tr>\n
M.Wt<\/td>\n668.39<\/td>\n<\/tr>\n
Cas No.<\/td>\n25535-16-4<\/td>\n<\/tr>\n
Formula<\/td>\nC27<\/sub>H34<\/sub>I2<\/sub>N4<\/sub><\/td>\n<\/tr>\n
Solubility<\/td>\ninsoluble in H2O; insoluble in EtOH; \u22659.84 mg\/mL in DMSO<\/td>\n<\/tr>\n
Chemical Name<\/td>\n3,8-diamino-5-(3-(diethyl(methyl)ammonio)propyl)-6-phenylphenanthridin-5-ium iodide<\/td>\n<\/tr>\n
SDF<\/td>\nDownload SDF<\/a><\/td>\n<\/tr>\n
Canonical SMILES<\/td>\nNC1=CC2=[N+](C(C3=CC=CC=C3)=C(C=C4N)C(C=C4)=C2C=C1)CCC[N+](CC)(C)CC.[I-].[I-]<\/td>\n<\/tr>\n
Shipping Condition<\/td>\nShip with blue ice, or upon other requests.<\/td>\n<\/tr>\n
General tips<\/td>\nFor obtaining a higher solubility, please warm the tube at 37\u00b0C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0References:<\/span><\/strong><\/p>\n

    \n
  1. Coder DM. Assessment of cell viability. Current Protocols in Cytometry, 2001, Chapter 9: Unit 9.2.<\/li>\n
  2. Hezel M, Ebrahimi F, Koch M, et al. Propidium iodide staining: a new application in fluorescence microscopy for analysis of cytoarchitecture in adult and developing rodent brain. Micron, 2012, 43(10): 1031-1038.<\/li>\n
  3. Nicoletti I, Migliorati G, Pagliacci MC, et al. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. Journal of Immunological Methods, 1991, 139(2): 271-279.<\/li>\n<\/ol>\n

    APExBIO\u53f0\u7063\u4ee3\u7406\u5546<\/a><\/span><\/strong><\/span><\/p>\n

    APExBIO\u63d0\u4f9b\u512a\u8cea\u7684\u80dc\u80bd\u548c\u751f\u7269\u5206\u6790\u8a66\u5291\uff0c\u5ee3\u6cdb\u7522\u54c1\u7dda\u5982Protease\u3001Chromatin\/Epigenetics\u8868\u89c0\u907a\u50b3\u5b78\u3001MAPK Signal\u3001DNA Damege\/DNA repair\u3001\u7d30\u80de\u51cb\u4ea1\u3001\u816b\u7624\u751f\u7269\u5b78\u3001Stem cell\u7b49\u7814\u7a76\u9818\u57df\uff0c \u9084\u63d0\u4f9b\u5b9a\u5236\u7684\u670d\u52d9\uff0c\u5305\u62ec\u591a\u80bd\u5408\u6210\uff0c\u6297\u9ad4\u7684\u751f\u7522\u548c\u6aa2\u6e2c\u7684\u767c\u5c55\u3002\u7522\u54c1\u7ddaDNA\/RNA prep kit\u3001Drug Screening panel\u3001Bioactive peptides\u3001Growth factors\u3001Biotinylation Reagents\u3001tag peptides\u3001amino Acids\u3001Growth factors\u7b49\u3002APExBIO\u7522\u54c1\u8cea\u91cf\u90fd\u4f34\u96a8\u8457\u8b49\u66f8\u7684\u5206\u6790\uff0c\u9ad8\u6548\u6db2\u76f8\u8272\u8b5c\uff0c\u8cea\u8b5c\uff0c\u548cHMNR\u6578\u64da\u3002<\/p>\n

    \u00a0<\/strong><\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    \u7898\u5316\u4e19\u5576(Propidium iodide, PI) \u6838\u9178\u67d3\u5291 | A red-fluorescent DNA […]<\/p>\n","protected":false},"author":3,"featured_media":34299,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[5204,5203],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37870"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=37870"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37870\/revisions"}],"predecessor-version":[{"id":40462,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37870\/revisions\/40462"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/34299"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=37870"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=37870"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=37870"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}