{"id":37796,"date":"2023-02-03T10:42:31","date_gmt":"2023-02-03T02:42:31","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=37796"},"modified":"2023-02-14T12:53:10","modified_gmt":"2023-02-14T04:53:10","slug":"antibody-pair-support-kit-sandwich-method-%e8%b2%a8%e8%99%9fabx098958","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=37796","title":{"rendered":"Antibody Pair Support Kit (Sandwich Method) |\u8ca8\u865fabx098958"},"content":{"rendered":"<p><strong><span style=\"color: #33cccc; font-size: 12pt;\"><a style=\"color: #33cccc;\" href=\"http:\/\/biopioneer.com.tw\/?p=37796\">Antibody Pair Support Kit (Sandwich Method) |\u8ca8\u865fabx098958<\/a>\u00a0<\/span><\/strong><\/p>\n<p>Antibody Pair Support Kit (Sandwich Method) contains reagents for use with an antibody pair for a Sandwich ELISA or CLIA.<\/p>\n<p><u>Kit contents:<\/u><\/p>\n<ul>\n<li>Coating Buffer: 1 vial, lyophilized powder<\/li>\n<li>Blocking Buffer: 1 vial, lyophilized powder<\/li>\n<li>Standard Diluent: 1 vial, lyophilized powder<\/li>\n<li>Detection Antibody Diluent: 1 vial, lyophilized powder<\/li>\n<li>Streptavidin-HRP Diluent: 1 vial, lyophilized powder<\/li>\n<li>Wash Buffer: 2 vials, lyophilized powder<\/li>\n<li>Streptavidin-HRP: 1 vial, 1.2 ml<\/li>\n<li>TMB Substrate Solution: 2 vials, 48 ml each<\/li>\n<li>Stop Solution: 2 vials, 30 ml each<\/li>\n<\/ul>\n<table class=\"table table-bordered table-responsive table-striped\">\n<tbody>\n<tr>\n<td>Reactivity<\/td>\n<td>General (All species)<\/td>\n<\/tr>\n<tr>\n<td>Tested Applications<\/td>\n<td>ELISA<\/td>\n<\/tr>\n<tr>\n<td>Recommended dilutions<\/td>\n<td>Streptavidin-HRP should be diluted 1\/100 using Streptavidin-HRP Diluent.<\/td>\n<\/tr>\n<tr>\n<td>Reconstitution<\/td>\n<td>Reconstitute and prepare working solutions before carrying out the assay. All working solutions should be colourless.<\/p>\n<ul>\n<li>Coating Buffer: Reconstitute in 220 ml distilled water.<\/li>\n<li>Blocking Buffer: Reconstitute in 240 ml distilled water.<\/li>\n<li>Standard Diluent: Reconstitute in 160 ml distilled water.<\/li>\n<li>Detection Antibody Diluent: Reconstitute in 120 ml distilled water.<\/li>\n<li>Streptavidin-HRP Diluent: Reconstitute in 120 ml distilled water.<\/li>\n<li>Wash Buffer: Reconstitute 1 vial in 2000 ml distilled water.<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<tr>\n<td>Storage<\/td>\n<td>Store Streptavidin-HRP at -20 \u00b0C. All other components can be stored at 4 \u00b0C for up to 6 months. Reconstituted and working (appropriately diluted) solutions should be used immediately but can be stored at 4 \u00b0C for up to one week. Avoid repeated freeze\/thaw cycles. It is recommended that solutions are made fresh before the experiment.<\/td>\n<\/tr>\n<tr>\n<td>Buffer<\/td>\n<td>The Stop Solution contains 0.1% sulphuric acid.<\/td>\n<\/tr>\n<tr>\n<td>Directions for use<\/td>\n<td>Bring all components to room temperature (18-25\u00b0C) and briefly spin or centrifuge the vials before use.<\/td>\n<\/tr>\n<tr>\n<td>Availability<\/td>\n<td>Shipped within 5-7 working days.<\/td>\n<\/tr>\n<tr>\n<td>Note<\/td>\n<td>This product is for research use only.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Antibody Pair Support Kit (Sandwich Method) |\u8ca8\u865fabx09895 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":33274,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[5195],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37796"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=37796"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37796\/revisions"}],"predecessor-version":[{"id":37798,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37796\/revisions\/37798"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/33274"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=37796"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=37796"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=37796"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}