{"id":37776,"date":"2023-02-02T14:05:09","date_gmt":"2023-02-02T06:05:09","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=37776"},"modified":"2023-10-31T13:34:51","modified_gmt":"2023-10-31T05:34:51","slug":"riboassure-rnase-%e6%8a%91%e8%a3%bd%e5%8a%91-bio-helix-ri001-0125-5000-u-40-u%ce%bcl","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=37776","title":{"rendered":"RNase \u6291\u5236\u5291 | BIO-HELIX – RI001-0125 (5000 U, 40 U\/\u03bcl)"},"content":{"rendered":"

RIBOAssure RNase \u00a0\u6291\u5236\u5291 | BIO-HELIX \u8ca8\u865fRI001-0125<\/span><\/strong><\/a><\/span>\u00a0(5000 U,\u00a0 40 U\/\u03bcl)<\/strong><\/span><\/span><\/p>\n

\"Bs5\"<\/p>\n

RIBOAssure RNase \u6291\u5236\u5291\u901a\u904e\u975e\u5171\u50f9\u7d50\u5408\u6291\u5236\u771f\u6838\u9176\u5982 RNase A\u3001RNase B \u548c RNase C \u6240\u8868\u73fe\u51fa\u7684\u6838\u7cd6\u6838\u9178\u9176 (RNase) \u6d3b\u6027\u3002RIBOAssure RNase \u6291\u88fd\u5291\u5c0d RNase 1\u3001RNase T1\u3001RNase T2\u3001S1 \u6838\u9178\u9176\u548c RNase H \u7f3a\u4e4f\u6d3b\u6027\u3002RIBOAssure RNase \u6291\u5236\u5291\u900f\u904e\u975e\u5171\u50f9\u7d50\u5408\u6291\u5236\u771f\u6838\u9176\uff08\u5982 RNase A\u3001RNase B \u548c RNase C\uff09\u6240\u8868\u73fe\u51fa\u7684\u6838\u7cd6\u6838\u9178\u9176 (RNase) \u6d3b\u6027\u3002\u5b83\u9069\u7528\u65bc\u4e0d\u9700\u8981 RNase \u5b58\u5728\u800c\u7121\u6cd5\u7372\u5f97\u6700\u4f73 RNA \u54c1\u8cea\u548c\u5be6\u9a57\u7d50\u679c\u7684\u61c9\u7528\uff0c\u4f8b\u5982 RNA \u5206\u96e2(RNA isolation)\u3001cDNA \u5408\u6210(cDNA synthesis)\u3001RT-PCR\u3001\u9ad4\u5916\u8f49\u9304(in vitro transcription)\u548c\u8f49\u8b6f( translation)\u6216\u7121 RNase \u55ae\u682a\u6297\u9ad4\u88fd\u5099\u3002 RIBOAssure RNase Inhibitor \u5c0d RNase 1\u3001RNase T1\u3001RNase T2\u3001S1 \u6838\u9178\u9176\u548c RNase H \u7f3a\u4e4f\u6d3b\u6027\u3002\u5f37\u70c8\u5efa\u8b70\u8207\u6211\u5011\u7684\u9ad8\u6027\u80fd RScript \u9006\u8f49\u9304\u9176(RScript Reverse Transcriptase)\u3001qPCR MasterMixes\u3001Taq DNA \u805a\u5408\u9176\u548c\/\u6216 2X PCR \u4e00\u8d77\u4f7f\u7528SuperMix \u7528\u65bc\u5f8c\u7e8c\u5be6\u9a57\u6b65\u9a5f\u8207\u61c9\u7528\u3002<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

Figure 1. Improved RNA stability in cDNA synthesis :<\/strong>
\nThe interference of RNase under the experimental conditions will reduce the cDNA quantity produced through RNA conversion, resulting in a decrease in the subsequent RT-qPCR\u2019s Ct value. After adding 4 units of RNase Inhibitor per reaction, the interference of RNase could be minimized.<\/p>\n

 <\/p>\n

\"\"<\/p>\n

 <\/p>\n

Catalog Number Size Concentration RI001-0125 5000 U 40 U\/\u03bcl<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
RK001-0050
\ncDNA\u5408\u6210KIT<\/td>\n		RScript\u2122 cDNA Synthesis Kit
\n(\u65b0\u4e00\u4ee3\u5de5\u7a0b\u91cd\u7d44 M-MLV \u9006\u8f49\u9304\u9176(M-MLV reverse transcriptase))
\nRScript cDNA \u5408\u6210\u8a66\u5291\u76d2(200 U\/\u03bcl)\u00a0<\/span><\/a><\/strong><\/td>\n		50 rxns<\/td>\n<\/tr>\n
RT001-0050
\nRT\u9006\u8f49\u9304\u9176<\/td>\n		RScript\u2122 Reverse Transcriptase 10,000U
\n(\u65b0\u4e00\u4ee3\u5de5\u7a0b\u91cd\u7d44 M-MLV \u9006\u8f49\u9304\u9176(M-MLV reverse transcriptase))
\n\u9006\u8f49\u9304\u9176 RScript Reverse Transcriptase<\/span><\/strong><\/a><\/td>\n		50 rxns
\n10,000U\/50\u03bcl<\/td>\n<\/tr>\n
RT001-0250
\nRT\u9006\u8f49\u9304\u9176<\/td>\n		RScript\u2122 Reverse Transcriptase 50,000U
\n(\u65b0\u4e00\u4ee3\u5de5\u7a0b\u91cd\u7d44 M-MLV \u9006\u8f49\u9304\u9176(M-MLV reverse transcriptase))
\n\u9006\u8f49\u9304\u9176RScript Reverse Transcriptase<\/span><\/strong><\/a><\/td>\n		250 rxns
\n50,000U\/250\u03bcl<\/td>\n<\/tr>\n
RI001-0125
\nRNase Inhibitor<\/td>\n		RIBOAssure\u2122 RNase Inhibitor 5,000U
\n(\u6291\u5236\u771f\u6838\u9176\u5982 RNase A\u3001RNase B \u548c RNase C \u6240\u8868\u73fe\u51fa\u7684\u6838\u7cd6\u6838\u9178\u9176 (RNase) \u6d3b\u6027)
\nRNase \u00a0\u6291\u5236\u5291<\/span><\/a><\/td>\n		125 rxns
\n5,000U\/125\u03bcl<\/td>\n<\/tr>\n
QPR01-0100
\n(Probe)<\/td>\n		PanProbes\u2122 One-Step RT-qPCR Kit (Probe)
\n(\u65b0\u4e00\u4ee3\u5de5\u7a0b\u91cd\u7d44 M-MLV \u9006\u8f49\u9304\u9176(M-MLV reverse transcriptase))
\n\u5f37\u5927\u7684 RNase \u6291\u5236\u5291\u6df7\u5408\u7269(RNase inhibitors mix)
\n\u589e\u5f37\u7684\u6297\u9ad4\u4ecb\u5c0e\u7684\u71b1\u555f\u52d5 DNA \u805a\u5408\u9176
\n(\u91dd\u5c0d\u57fa\u65bc\u63a2\u91dd\u7684real-time PCR \u9032\u884c\u4e86\u512a\u5316)
\n\u4e00\u6b65\u9a5f RT-qPCR <\/span><\/strong>\u8a66\u5291\u76d2<\/strong><\/span><\/a><\/td>\n		100 reactions
\n(20 \u03bcl vol)<\/td>\n<\/tr>\n
QPD01-0100
\n(Probe)<\/td>\n		PanProbes\u2122 Universal qPCR MasterMix
\n(\u91dd\u5c0d\u57fa\u65bcprobe- PCR (probe-based real-time PCR )\u9032\u884c\u4e86\u512a\u5316)
\n\u589e\u5f37\u7684\u6297\u9ad4\u4ecb\u5c0e\u7684\u71b1\u555f\u52d5 DNA \u805a\u5408\u9176
\nPanProbes<\/span><\/strong>\u2122 Universal qPCR<\/strong>\u9810\u6df7\u6db2<\/strong><\/span><\/a><\/td>\n		100 reactions
\n(20 \u03bcl vol)<\/td>\n<\/tr>\n
QSR01-0100
\n(SYBR\u00ae Green)<\/td>\n		PanGreen\u2122 One-Step RT-qPCR Kit (SYBR\u00ae Green)
\n(\u65b0\u4e00\u4ee3\u5de5\u7a0b\u91cd\u7d44 M-MLV \u9006\u8f49\u9304\u9176(M-MLV reverse transcriptase))
\n\u5f37\u5927\u7684 RNase \u6291\u5236\u5291\u6df7\u5408\u7269(RNase inhibitors mix)
\n\u589e\u5f37\u7684\u6297\u9ad4\u4ecb\u5c0e\u7684\u71b1\u555f\u52d5 DNA \u805a\u5408\u9176
\nUniversal SYBR\u00ae Green Master Mix\u00a0 2 \u500d\u6fc3\u7e2e\u7684\u5373\u7528\u578b\u6df7\u5408\u6db2 Master Mix
\n\u4e00\u500b\u6b65\u9a5fRT-qPCR <\/span><\/strong>\u8a66\u5291\u76d2PanGreen<\/strong><\/span><\/a><\/td>\n		100 reactions
\n(20 \u03bcl vol)<\/td>\n<\/tr>\n
QSD01-0100
\nSYBR\u00ae Green)<\/td>\n		PanGreen\u2122 Universal SYBR\u00ae Green Mastermix (SYBR\u00ae Green)
\n(2 \u500d\u6fc3\u7e2e\u7684\u5373\u7528\u578b Master Mix \u6df7\u5408\u6db2)
\n\u589e\u5f37\u7684\u6297\u9ad4\u4ecb\u5c0e\u7684\u71b1\u555f\u52d5 DNA \u805a\u5408\u9176
\n\u5373\u7528\u578b qPCR <\/span><\/strong>\u6df7\u5408\u6db2PanGreen<\/strong><\/span><\/a><\/td>\n		100 reactions
\n(20 \u03bcl vol)<\/td>\n<\/tr>\n
PDR03-0100<\/td>\nTotal RNA Isolation Reagent
\n(Reagent Based) for the tissue, cultured animal and bacterial cells, blood, and serum.
\nTotal RNA <\/span><\/strong>\u5206\u96e2\u8a66\u5291 (Reagent Based<\/strong>\uff09<\/strong><\/span><\/a><\/td>\n		100 rxns<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

RIBOAssure RNase \u00a0\u6291\u5236\u5291 | BIO-HELIX \u8ca8\u865fRI001-0125\u00a0(5000 U, […]<\/p>\n","protected":false},"author":3,"featured_media":37777,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5372,3],"tags":[5191,5190],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37776"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=37776"}],"version-history":[{"count":6,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37776\/revisions"}],"predecessor-version":[{"id":46611,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37776\/revisions\/46611"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/37777"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=37776"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=37776"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=37776"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}