{"id":37765,"date":"2023-02-02T12:06:23","date_gmt":"2023-02-02T04:06:23","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=37765"},"modified":"2023-02-07T14:50:18","modified_gmt":"2023-02-07T06:50:18","slug":"%e8%9b%8b%e7%99%bd%e9%85%b6-k-proteinase-k-cas39450-01-6-appexa-%e8%b2%a8%e8%99%9f-abx082041","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=37765","title":{"rendered":"\u86cb\u767d\u9176 K (Proteinase K) | CAS#39450-01-6 Abbexa \u8ca8\u865f abx082041"},"content":{"rendered":"

\u86cb\u767d\u9176 K (Proteinase K) | CAS#39450-01-6\u00a0Abbexa\u00a0 \u8ca8\u865f\u00a0abx082041<\/a><\/span><\/p>\n

\u5206\u5b50\u751f\u7269\u61c9\u7528\u5efa\u8b70\u91cf: 50-100 \u00b5g\/ml<\/p>\n

\u86cb\u767d\u9176 K (Proteinase K)\u662f\u4e00\u7a2e\u5ee3\u8b5c(broad spectrum) \u7d72\u6c28\u9178\u86cb\u767d\u9176(serine protease)\uff0c\u5728 DNA \u63d0\u53d6\u65b9\u6848\u4e2d\u7528\u65bc\u6d88\u5316\u6c61\u67d3\u86cb\u767d\u8cea(contaminating proteins)\uff0c\u5c24\u5176\u662f DNA \u9176(DNases)\u3002\u8a72\u9176\u88c2\u89e3\u86cb\u767d\u8cea\u4e2d\u8207\u8102\u80aa\u65cf(aliphatic)\u548c\u82b3\u9999\u65cf\u6c28\u57fa\u9178\u6b98\u57fa(aromatic amino acid residues)\u7684\u7fa7\u57fa\u76f8\u9130\u7684\u80bd\u9375(peptide bonds)\u3002\u86cb\u767d\u9176 K(Proteinase K) \u7684\u4e3b\u8981\u61c9\u7528\u662f\u5728 DNA \u548c RNA \u63d0\u53d6(RNA extraction)\u4e2d\u7684\u61c9\u7528\u3002\u5728\u5927\u591a\u6578\u6838\u9178\u63d0\u53d6 (nucleic acid extraction)\u65b9\u6848\u4e2d\uff0c\u86cb\u767d\u9176 K (Proteinase K)\u88ab\u6dfb\u52a0\u5230\u7d44\u7e54(tissue)\u6216\u88c2\u89e3\u7269(the lysate)\u4e2d\uff0c\u4ee5\u78ba\u4fdd\u5728\u6838\u9178\u63d0\u53d6\u904e\u7a0b\u4e2d\u5b8c\u5168\u6d88\u5316\u86cb\u767d\u8cea\uff0c\u5c24\u5176\u662f DNase \u548c RNase\u3002\u6d88\u5316\u86cb\u767d\u8cea(digest proteins)\u7684\u80fd\u529b\u4e5f\u4f7f\u86cb\u767d\u9176 K \u53ef\u7528\u65bc\u88fd\u5099\u7528\u65bc\u8108\u885d\u5834\u51dd\u81a0\u96fb\u6cf3 (pulsed-field gel electrophoresis)\u3001\u86cb\u767d\u8cea\u6307\u7d0b(protein fingerprinting)\u3001\u86cb\u767d\u9176\u8db3\u5370(protease foot printing)\u3001\u7dda\u7c92\u9ad4\u5206\u96e2(mitochondrial isolation)\u3001\u57fa\u56e0\u7d44 DNA (genomic DNA isolation)\u5206\u96e2\u7b49\u7684\u67d3\u8272\u9ad4 DNA(chromosomal DNA)\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Target<\/td>\nProteinase K<\/td>\n<\/tr>\n
Expression<\/td>\nRecombinant<\/td>\n<\/tr>\n
Host<\/td>\nYeast<\/td>\n<\/tr>\n
Recommended dilutions<\/td>\nMolecular biology applications: 50-100 \u00b5g\/ml. Optimal dilutions\/concentrations should be determined by the end user.<\/td>\n<\/tr>\n
Conjugation<\/td>\nUnconjugated<\/td>\n<\/tr>\n
Form<\/td>\nLyophilized<\/td>\n<\/tr>\n
Purity<\/td>\n\u2265 95% (SDS-PAGE)
\nFree from DNase and RNase.<\/td>\n<\/tr>\n
Reconstitution<\/td>\nReconstitute in a 50% glycerin solution. Ensure that the powder has fully dissolved before use. Use immediately after preparation, or store at 2-8 \u00b0C. For long-term storage, reconstitute to a concentration of 10-20 mg\/ml and store at -20 \u00b0C.<\/td>\n<\/tr>\n
Storage<\/td>\nStore between 2-8 \u00b0C for up to 2 years.<\/td>\n<\/tr>\n
Enzyme Commission Number<\/td>\n3.4.21.64<\/a><\/td>\n<\/tr>\n
Molecular Weight<\/td>\n29 kDa<\/td>\n<\/tr>\n
Buffer<\/td>\nPrior to lyophilization: 10 mM Tris-HCl, pH 7.5, containing 5 mM CaCl2<\/sub>.<\/td>\n<\/tr>\n
CAS Number<\/td>\n39450-01-6<\/td>\n<\/tr>\n
Biological Activity<\/td>\n\u2265 30 U\/mg
\n1 Unit (U) is defined as the amount of Proteinase K required to produce 1 \u00b5mol of Folin-positive amino acids using hemoglobin as the substrate, in 1 min at 37 \u00b0C.<\/td>\n<\/tr>\n
Directions for use<\/td>\nEffective pH range: 4-12.5
\nOptimal pH range: 7.5-8.0
\nOptimal reaction temperature: 50-55 \u00b0C<\/td>\n<\/tr>\n
Availability<\/td>\nShipped within 5-10 working days.<\/td>\n<\/tr>\n
Note<\/td>\nThis product is for research use only.Not for human consumption, cosmetic, therapeutic or diagnostic use.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u86cb\u767d\u9176 K (Proteinase K) | CAS#39450-01-6\u00a0Abbexa\u00a0 \u8ca8\u865f\u00a0abx082 […]<\/p>\n","protected":false},"author":3,"featured_media":33277,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[5189],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37765"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=37765"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37765\/revisions"}],"predecessor-version":[{"id":38062,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37765\/revisions\/38062"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/33277"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=37765"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=37765"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=37765"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}