{"id":37632,"date":"2023-01-30T12:35:20","date_gmt":"2023-01-30T04:35:20","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=37632"},"modified":"2023-02-05T19:33:54","modified_gmt":"2023-02-05T11:33:54","slug":"%e8%83%b0%e5%b3%b6%e7%b4%a0-elisa-ins%e8%a9%a6%e5%8a%91%e7%9b%92-%e9%85%b6%e8%81%af%e5%85%8d%e7%96%ab%e5%90%b8%e9%99%84%e8%a9%a6%e9%a9%97%e6%b3%95-%e8%b2%a8%e8%99%9fcea448hu","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=37632","title":{"rendered":"\u80f0\u5cf6\u7d20 ELISA (INS)\u8a66\u5291\u76d2 (\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5) | \u8ca8\u865fCEA448Hu"},"content":{"rendered":"

\u80f0\u5cf6\u7d20 ELISA (INS)\u8a66\u5291\u76d2 (\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5) | \u8ca8\u865fCEA448Hu<\/a><\/span><\/strong><\/span><\/p>\n

ELISA Kit for Insulin (INS)<\/p>\n

\u5546\u54c1\u539f\u7406\u4ecb\u7d39:<\/span><\/strong><\/span> \u80f0\u5cf6\u7d20 ELISA (INS)\u8a66\u5291\u76d2 (\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5) <\/u>| \u8ca8\u865fCEA448Hu (Competitive Inhibition)\uff0c\u5075\u6e2c\u7684\u9748\u654f\u5ea661.7-5,000pg\/mL\u3001\u6700\u4f4e\u9748\u654f\u5ea622.5pg\/mL\u3002ELISA\u539f\u7406\u70ba\u5229\u7528\u6297\u9ad4\u8207\u6297\u539f\u4e4b\u9593\u7684\u9ad8\u5ea6\u5c08\u4e00\u6027\uff0c\u505a\u70ba\u5b9a\u91cf\u6a19\u8a8c\u7684\u65b9\u6cd5\uff1b\u7121\u8ad6\u6e2c\u91cf\u6297\u539f\u6216\u6e2c\u91cf\u6297\u9ad4\u90fd\u6709\u5f88\u9ad8\u7684\u9748\u654f\u5ea6\u8ddf\u5c08\u4e00\u6027\u3002\u8a72\u6aa2\u6e2c\u65b9\u6cd5\u5c0d\u6aa2\u6e2c\u8840\u7ba1\u5167\u76ae\u751f\u9577\u56e0\u5b50 165 (VEGF165) \u5177\u6709\u9ad8\u9748\u654f\u5ea6\u548c\u51fa\u8272\u7684\u7279\u7570\u6027\u3002\u672a\u89c0\u5bdf\u5230\u8840\u7ba1\u5167\u76ae\u751f\u9577\u56e0\u5b50 165 (VEGF165) \u548c\u985e\u4f3c\u7269\u4e4b\u9593\u7684\u986f\u8457\u4ea4\u53c9\u53cd\u61c9\u6216\u5e72\u64fe\u3002Cloud-Clone corp\u63d0\u4f9b\u9ad8\u9748\u654f\u5ea6\u4e4bELISA (\u9175\u7d20\u514d\u75ab\u5206\u6790\u5957\u7d44\/\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5)\uff0c\u5c08\u9580\u751f\u7522\u7814\u7a76\u7528\u6aa2\u6e2c\u8a66\u5291\u7684\u751f\u6280\u516c\u53f8\uff0c\u65bc\u7f8e\u570b\u7814\u767c\u548c\u751f\u7522\uff0c\u7522\u54c1\u7dda\u5305\u62ecELISA Kit\u3001CLIA Kit\u3001Antibody\u53carecombinant protein\uff1bELISA\u7522\u54c1\u7cfb\u5217\u8d85\u904e6000\u7a2e\u4ee5\u4e0a\uff0c\u7269\u7a2e\u9f4a\u5168\u5305\u62ecrat\u3001mouse\u3001rabbit\u3001cavia\u3001human\u3001porcine\u3001bovine\u3001simian\u7b49\u591a\u7269\u7a2e\u5546\u54c1\u3002\u54c1\u8cea\u4fdd\u8b49\u3002ELISA\u5546\u54c1\u7684\u578b\u5f0f\u591a\u5143\uff0c \u63d0\u4f9b\u4e09\u660e\u6cbbELISA\u3001\u7af6\u722d\u578bELISA\u3001\u9ad8\u9748\u654f\u5ea6\u8a2d\u8a08ELISA\u3002Uncoated<\/u><\/strong> ELISA<\/u><\/strong>\u578b\u5f0f<\/u><\/strong>\uff1b\u5305\u62ecCapture Antibody, Detection Antibody\u3001Standard\u3001Streptavidin-HRP\u3001TMB Substrate, 96-well Plate\u3002<\/p>\n

\"\u64cd\u4f5c\u65b9\u6cd5\"<\/a><\/strong><\/p>\n

\u00a0\u80f0\u5cf6\u7d20 ELISA (INS)\u8a66\u5291\u76d2 (\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5)\u5957\u7d44\u5167\u5bb9<\/span><\/strong><\/p>\n\n\n\n\n\n\n\n\n\n
Reagents<\/td>\nQuantity<\/td>\nReagents<\/td>\nQuantity<\/td>\n<\/tr>\n
Pre-coated, ready to use 96-well strip plate<\/td>\n1<\/td>\nPlate sealer for 96 wells<\/td>\n4<\/td>\n<\/tr>\n
Standard<\/td>\n2<\/td>\nStandard Diluent<\/td>\n1\u00d720mL<\/td>\n<\/tr>\n
Detection Reagent A<\/td>\n1\u00d7120\u00b5L<\/td>\nAssay Diluent A<\/td>\n1\u00d712mL<\/td>\n<\/tr>\n
Detection Reagent B<\/td>\n1\u00d7120\u00b5L<\/td>\nAssay Diluent B<\/td>\n1\u00d712mL<\/td>\n<\/tr>\n
TMB Substrate<\/td>\n1\u00d79mL<\/td>\nStop Solution<\/td>\n1\u00d76mL<\/td>\n<\/tr>\n
Wash Buffer (30 \u00d7 concentrate)<\/td>\n1\u00d720mL<\/td>\nInstruction manual<\/td>\n1<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0\u80f0\u5cf6\u7d20 ELISA (INS)\u8a66\u5291\u76d2\u7c21\u55ae\u6d41\u7a0b<\/span><\/strong><\/p>\n

    \n
  1. Prepare all reagents, samples and standards;
    \n2. Add 50\u00b5L standard or sample to each well.
    \nAnd then add 50\u00b5L prepared Detection Reagent A immediately.
    \nShake and mix. Incubate 1 hour at 37\u00b0C;
    \n3. Aspirate and wash 3 times;
    \n4. Add 100\u00b5L prepared Detection Reagent B. Incubate 30 minutes at 37\u00b0C;
    \n5. Aspirate and wash 5 times;
    \n6. Add 90\u00b5L Substrate Solution. Incubate 10-20 minutes at 37\u00b0C;
    \n7. Add 50\u00b5L Stop Solution. Read at 450 nm immediately.<\/li>\n<\/ol>\n

    \u00a0\u80f0\u5cf6\u7d20 ELISA (INS)\u8a66\u5291\u76d2\u76f8\u95dc\u4e4b\u6297\u9ad4 | \u86cb\u767d\u8cea | ELISA | CLIA\u7522\u54c1<\/span><\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
    CCA836Mu<\/td>\n\u4fc3\u814e\u4e0a\u817a\u76ae\u8cea\u6fc0\u7d20(ACTH)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Adrenocorticotropic Hormone (ACTH)<\/td>\n<\/tr>\n
    CCA836Ra<\/td>\n\u4fc3\u814e\u4e0a\u817a\u76ae\u8cea\u6fc0\u7d20(ACTH)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Adrenocorticotropic Hormone (ACTH)<\/td>\n<\/tr>\n
    CCA851Ge<\/td>\n\u591a\u5df4\u80fa(DA)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Dopamine (DA)<\/td>\n<\/tr>\n
    CCA856Hu<\/td>\n\u611b\u5e15\u7433\u80bd12(AP12)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Apelin 12 (AP12)<\/td>\n<\/tr>\n
    CCA866Ra<\/td>\n\u7532\u72c0\u65c1\u817a\u6fc0\u7d20(PTH)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Parathyroid Hormone (PTH)<\/td>\n<\/tr>\n
    CCA876Hu<\/td>\n\u964d\u9223\u7d20\u57fa\u56e0\u76f8\u95dc\u80bd(CGRP)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Calcitonin Gene Related Peptide (CGRP)<\/td>\n<\/tr>\n
    CCA876Mu<\/td>\n\u964d\u9223\u7d20\u57fa\u56e0\u76f8\u95dc\u80bd(CGRP)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Calcitonin Gene Related Peptide (CGRP)<\/td>\n<\/tr>\n
    CCA876Ra<\/td>\n\u964d\u9223\u7d20\u57fa\u56e0\u76f8\u95dc\u80bd(CGRP)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Calcitonin Gene Related Peptide (CGRP)<\/td>\n<\/tr>\n
    CCA921Ge<\/td>\n\u7dad\u751f\u7d20D2(VD2)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Vitamin D2 (VD2)<\/td>\n<\/tr>\n
    CCA922Ge<\/td>\n\u03b1-\u751f\u80b2\u915a(TCP\u03b1)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Alpha-Tocopherol (TCPa)<\/td>\n<\/tr>\n
    CCA968Bo<\/td>\n\u6291\u80bd\u9176(AP)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Aprotinin (AP)<\/td>\n<\/tr>\n
    CCA991Ga<\/td>\n\u9951\u9913\u7d20(GHRL)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Ghrelin (GHRL)<\/td>\n<\/tr>\n
    CCB003Ge<\/td>\n\u96cc\u916e(E1)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Estrone (E1)<\/td>\n<\/tr>\n
    CCB028Hu<\/td>\n\u767d\u86cb\u767d(ALB)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Albumin (ALB)<\/td>\n<\/tr>\n
    CCB028Mu<\/td>\n\u767d\u86cb\u767d(ALB)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Albumin (ALB)<\/td>\n<\/tr>\n
    CCB028Ra<\/td>\n\u767d\u86cb\u767d(ALB)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Albumin (ALB)<\/td>\n<\/tr>\n
    CCB084Hu<\/td>\n\u7518\u4e19\u80bd(GAL)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Galanin (GAL)<\/td>\n<\/tr>\n
    CCB084Ra<\/td>\n\u7518\u4e19\u80bd(GAL)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Galanin (GAL)<\/td>\n<\/tr>\n
    CCB139Hu<\/strong><\/td>\n\u6297\u5229\u5c3f\u6fc0\u7d20(ADH)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/strong><\/td>\nCLIA Kit for Antidiuretic Hormone (ADH)<\/strong><\/td>\n<\/tr>\n
    CCB139Mu<\/td>\n\u6297\u5229\u5c3f\u6fc0\u7d20(ADH)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Antidiuretic Hormone (ADH)<\/td>\n<\/tr>\n
    CCB409Hu<\/td>\n\u8840\u7d05\u86cb\u767d(HB)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Hemoglobin (HB)<\/td>\n<\/tr>\n
    CCB459Ge<\/td>\n\u5375\u767d\u86cb\u767d(OVA)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Ovalbumin (OVA)<\/td>\n<\/tr>\n
    CCB938Ge<\/td>\n\u7cbe\u6c28\u9178(Arg)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Arginine (Arg)<\/td>\n<\/tr>\n
    CCC918Ra<\/td>\n\u6210\u7e96\u7dad\u7d30\u80de\u751f\u9577\u56e0\u657821(FGF21)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Fibroblast Growth Factor 21 (FGF21)<\/td>\n<\/tr>\n
    CCD053Ge<\/td>\n\u7dad\u751f\u7d20B1(VB1)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Vitamin B1 (VB1)<\/td>\n<\/tr>\n
    CCO007Ge<\/td>\n\u7dad\u751f\u7d20d3-23\u7fa7\u9178(CA)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u5316\u5b78\u767c\u5149\u514d\u75ab\u5206\u6790\u6cd5)<\/td>\nCLIA Kit for Calcitroic Acid (CA)<\/td>\n<\/tr>\n
    CEA001Hu<\/td>\n\u555f\u52d5\u7d20A(ACVA)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5)<\/td>\nELISA Kit for Activin A (ACVA)<\/td>\n<\/tr>\n
    CEA001Mu<\/td>\n\u555f\u52d5\u7d20A(ACVA)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5)<\/td>\nELISA Kit for Activin A (ACVA)<\/td>\n<\/tr>\n
    CEA001Ra<\/td>\n\u555f\u52d5\u7d20A(ACVA)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5)<\/td>\nELISA Kit for Activin A (ACVA)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

     <\/p>\n

     <\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    \u80f0\u5cf6\u7d20 ELISA (INS)\u8a66\u5291\u76d2 (\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5) | \u8ca8\u865fCEA448Hu ELISA Kit f […]<\/p>\n","protected":false},"author":3,"featured_media":16078,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[452,3],"tags":[5172],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37632"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=37632"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37632\/revisions"}],"predecessor-version":[{"id":37633,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37632\/revisions\/37633"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/16078"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=37632"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=37632"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=37632"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}